scholarly journals Hypoxia Preconditioned Serum (HPS)-Hydrogel Can Accelerate Dermal Wound Healing in Mice—An In Vivo Pilot Study

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 176
Author(s):  
Jun Jiang ◽  
Ursula Kraneburg ◽  
Ulf Dornseifer ◽  
Arndt F. Schilling ◽  
Ektoras Hadjipanayi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Lymphatic Vessels ◽  
Scar Tissue ◽  
Tissue Samples ◽  
Medicine Research ◽  
Dermal Wound Healing ◽  
Wound Model ◽  
Dermal Wound

The ability to use the body’s resources to promote wound repair is increasingly becoming an interesting area of regenerative medicine research. Here, we tested the effect of topical application of blood-derived hypoxia preconditioned serum (HPS) on wound healing in a murine wound model. Alginate hydrogels loaded with two different HPS concentrations (10 and 40%) were applied topically on full-thickness wounds created on the back of immunocompromised mice. We achieved a significant dose-dependent wound area reduction after 5 days in HPS-treated groups compared with no treatment (NT). On average, both HPS-10% and HPS-40% -treated wounds healed 1.4 days faster than NT. Healed tissue samples were investigated on post-operative day 15 (POD 15) by immunohistology and showed an increase in lymphatic vessels (LYVE-1) up to 45% with HPS-40% application, while at this stage, vascularization (CD31) was comparable in the HPS-treated and NT groups. Furthermore, the expression of proliferation marker Ki67 was greater on POD 15 in the NT-group compared to HPS-treated groups, in accordance with the earlier completion of wound healing observed in the latter. Collagen deposition was similar in all groups, indicating lack of scar tissue hypertrophy as a result of HPS-hydrogel treatment. These findings show that topical HPS application is safe and can accelerate dermal wound healing in mice.

Systemic Isotretinoin: Effects on Dermal Wound Healing in a Rabbit Ear Model In Vivo

1990 ◽  
Vol 16 (12) ◽  
pp. 1142-1146 ◽  
Author(s):  
RONALD L. MOY ◽  
LARRY S. MOY ◽  
RICHARD G. BENNETT ◽  
JOHN A. ZITELLI ◽  
JOUNI UITTO
Keyword(s):  
Wound Healing ◽  
Dermal Wound Healing ◽  
Rabbit Ear ◽  
Dermal Wound

scholarly journals Fibroblast migration and collagen deposition during dermal wound healing: mathematical modelling and clinical implications

2006 ◽  
Vol 364 (1843) ◽  
pp. 1385-1405 ◽  
Author(s):  
Steven McDougall ◽  
John Dallon ◽  
Jonathan Sherratt ◽  
Philip Maini
Keyword(s):  
Wound Healing ◽  
Diffusion Coefficient ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Collagen Deposition ◽  
Dermal Wound Healing ◽  
Fibroblast Migration ◽  
Collagen Alignment ◽  
Dermal Wound ◽  
Scar Tissue Formation

The extent to which collagen alignment occurs during dermal wound healing determines the severity of scar tissue formation. We have modelled this using a multiscale approach, in which extracellular materials, for example collagen and fibrin, are modelled as continua, while fibroblasts are considered as discrete units. Within this model framework, we have explored the effects that different parameters have on the alignment process, and we have used the model to investigate how manipulation of transforming growth factor-β levels can reduce scar tissue formation. We briefly review this body of work, then extend the modelling framework to investigate the role played by leucocyte signalling in wound repair. To this end, fibroblast migration and collagen deposition within both the wound region and healthy peripheral tissue are considered. Trajectories of individual fibroblasts are determined as they migrate towards the wound region under the combined influence of collagen/fibrin alignment and gradients in a paracrine chemoattractant produced by leucocytes. The effects of a number of different physiological and cellular parameters upon the collagen alignment and repair integrity are assessed. These parameters include fibroblast concentration, cellular speed, fibroblast sensitivity to chemoattractant concentration and chemoattractant diffusion coefficient. Our results show that chemoattractant gradients lead to increased collagen alignment at the interface between the wound and the healthy tissue. Results show that there is a trade-off between wound integrity and the degree of scarring. The former is found to be optimized under conditions of a large chemoattractant diffusion coefficient, while the latter can be minimized when repair takes place in the presence of a competitive inhibitor to chemoattractants.

Antioxidant, antibacterial and in vivo dermal wound healing effects of Opuntia flower extracts

2015 ◽  
Vol 81 ◽  
pp. 483-490 ◽  
Author(s):  
Imene Ammar ◽  
Sana Bardaa ◽  
Massara Mzid ◽  
Zouheir Sahnoun ◽  
Tarak Rebaii ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Wound Healing ◽  
Dermal Wound

scholarly journals Development of an in-situ forming, self-healing scaffold for dermal wound healing: in-vivo and in-vivo studies

2021 ◽  
pp. 112263
Author(s):  
Swati Sharma ◽  
Harishkumar Madhyastha ◽  
K. Laxmi Swetha ◽  
Kavya Sree Maravajjala ◽  
Archana Singh ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Studies ◽  
Self Healing ◽  
Dermal Wound Healing ◽  
In Situ Forming ◽  
Dermal Wound

scholarly journals In vivo evaluation of an electrospun and 3D printed cellular delivery device for dermal wound healing

2020 ◽  
Vol 108 (6) ◽  
pp. 2560-2570 ◽  
Author(s):  
Ryan M. Clohessy ◽  
David J. Cohen ◽  
Karolina Stumbraite ◽  
Barbara D. Boyan ◽  
Zvi Schwartz
Keyword(s):  
Wound Healing ◽  
Cellular Delivery ◽  
Delivery Device ◽  
In Vivo Evaluation ◽  
Dermal Wound Healing ◽  
3D Printed ◽  
Dermal Wound

scholarly journals B2 agonist (Salbutamol) modulate skin wound healing processes

2018 ◽  
Vol 9 (1) ◽  
pp. 37
Author(s):  
Fahim M. Mahmood ◽  
Hayder B. Sahib ◽  
Khalid W. Qassim
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Cell Types ◽  
Skin Wound ◽  
Control Group ◽  
Related Factors ◽  
Skin Wound Healing ◽  
Wound Model ◽  
Numerous Cell

Wound healing is a complex physiological and dynamic process required the coordination of numerous cell types and biological processes to regenerate damaged tissue and initiate repair which is dependent on a number of inter-related factors. This study was aimed to demonstrate whether the ?2 receptor has role in wound healing and angiogenesis. A murine wild-type (in vivo), excisional skin wound model was done to demonstrate that activation of ?2AR delay wound repair, twenty-four male albino mice were used to investigate the effect of the drug on experimental wound healing grossly, histo-pathologically and immune-histochemically compared with vehicle-only controls. The results showed that the rate of wound healing was significantly slower in salbutamol group than in control group (P

scholarly journals Discovery and Characterization of a High-Affinity Small Peptide Ligand, H1, Targeting FGFR2IIIc for Skin Wound Healing

2018 ◽  
Vol 49 (3) ◽  
pp. 1074-1089 ◽  
Author(s):  
Ying Zhao ◽  
Qiang Wang ◽  
Yuan Jin ◽  
Yadan Li ◽  
Changjun Nie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Chorioallantoic Membrane ◽  
Skin Wound ◽  
Small Peptide ◽  
Skin Wound Healing ◽  
Wound Model

Background/Aims: How to aid recovery from severe skin injuries, such as burns, chronic or radiation ulcers, and trauma, is a critical clinical problem. Current treatment methods remain limited, and the discovery of ideal wound-healing therapeutics has been a focus of research. Functional recombinant proteins such as basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) have been developed for skin repair, however, some disadvantages in their use remain. This study reports the discovery of a novel small peptide targeting fibroblast growth factor receptor 2 IIIc (FGFR2IIIc) as a potential candidate for skin wound healing. Methods: A phage-displayed peptide library was used for biopanning FGFR2IIIc-targeting small peptides. The selected small peptides binding to FGFR2IIIc were qualitatively evaluated by an enzyme-linked immunosorbent assay. Their biological function was detected by a cell proliferation assay. Among them, an optimized small peptide named H1 was selected for further study. The affinity of the H1 peptide and FGFR2IIIc was determined by an isothermal titration calorimetry device. The ability of theH1 peptide to promote skin wound repair was investigated using an endothelial cell tube formation assay and wound healing scratch assay in vitro. Subsequently, the H1 peptide was assessed using a rat skin full-thickness wound model and chorioallantoic membrane (CAM) assays in vivo. To explore its molecular mechanisms, RNA-Seq, quantitative real-time PCR, and western blot assays were performed. Computer molecular simulations were also conducted to analyze the binding model. Results: We identified a novel FGFR2IIIc-targeting small peptide, called H1, with 7 amino acid residues using phage display. H1 had high binding affinity with FGFR2IIIc. The H1 peptide promoted the proliferation and motility of fibroblasts and vascular endothelial cells in vitro. In addition, the H1 peptide enhanced angiogenesis in the chick chorioallantoic membrane and accelerated wound healing in a rat full-thickness wound model in vivo. The H1 peptide activated both the PI3K-AKT and MAPK-ERK1/2 pathways and simultaneously increased the secretion of vascular endothelial growth factor. Computer analysis demonstrated that the model of H1 peptide binding to FGFR2IIIc was similar to that of FGF2 and FGFR2IIIc. Conclusion: The H1 peptide has a high affinity for FGFR2IIIc and shows potential as a wound healing agent. As a substitute for bFGF, it could be developed into a novel therapeutic candidate for skin wound repair in the future.

scholarly journals Immunologic Roles of Hyaluronan in Dermal Wound Healing

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1234
Author(s):  
Aditya Kaul ◽  
Walker D. Short ◽  
Sundeep G. Keswani ◽  
Xinyi Wang
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Tissue Repair ◽  
Wound Repair ◽  
Low Molecular Weight ◽  
Immune Systems ◽  
Dermal Wound Healing ◽  
Adaptive Immune ◽  
Adaptive Immune Systems ◽  
Dermal Wound

Hyaluronic acid (HA), a glycosaminoglycan ubiquitous in the skin, has come into the limelight in recent years for its role in facilitating dermal wound healing. Specifically, HA’s length of linearly repeating disaccharides—in other words, its molecular weight (MW)—determines its effects. High molecular weight (HMW)-HA serves an immunosuppressive and anti-inflammatory role, whereas low molecular weight (LMW)-HA contributes to immunostimulation and thus inflammation. During the inflammatory stage of tissue repair, direct and indirect interactions between HA and the innate and adaptive immune systems are of particular interest for their long-lasting impact on wound repair. This review seeks to synthesize the literature on wound healing with a focus on HA’s involvement in the immune subsystems.

scholarly journals Effect of Pulsed 810 nm Laser Photobiomodulation on Dermal Wound Healing and Oxidative Stress in Immunosuppressed Rats

2021 ◽  
Vol 6 (2) ◽  
pp. 122-127
Author(s):  
Gaurav K. Keshri ◽  
Saurabh Verma ◽  
Asheesh Gupta
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Illumination Time ◽  
Dermal Wound Healing ◽  
Pulsed Mode ◽  
Penetration Ability ◽  
And Oxidative Stress ◽  
Dermal Wound

Under immunosuppression, the sequential overlapping wound repair phases get hampered due to dysregulated or persistent inflammation leading to non-healing chronic wounds formation. The present study investigates the effect of low-power 810 nm diode laser (70 mW mean output power; 40 mW/cm2 average irradiance; 24 J/cm2 total fluence; 10 Hz pulse frequency; duty cycle 50 per cent; 10 min. illumination time once daily for seven days) photobiomodulation (PBM) on dermal penetration ability, wound healing and oxidative stress in hydrocortisone-induced immunosuppressed rats. The results of the penetration ability of 810 nm laser irradiation to the depth of the sub-dermal region revealed that the transmitted power of laser at 10 Hz pulsed-mode was better and easier than continuous-mode. The present findings clearly delineated that PBM with 810 nm laser at 10 Hz significantly augmented healing and reduced oxidative stress as evidenced by decreased free radicals, nitric oxide (NO) levels, enhanced superoxide dismutase (SOD) enzyme activity and wound area contraction facilitating the cellular redox homeostasis and promoting the tissue repair process. In conclusion, PBM with NIR 810 nm laser at pulsed-mode 10 Hz frequency showed better penetration and accelerated dermal wound healing in immunosuppressed rats.

A gene-activating skin substitute comprising PLLA/POSS nanofibers and plasmid DNA encoding ANG and bFGF promotes in vivo revascularization and epidermalization

2018 ◽  
Vol 6 (43) ◽  
pp. 6977-6992 ◽  
Author(s):  
Wenqiang Li ◽  
Dongwei Wu ◽  
Jianwang Tan ◽  
Zhibin Liu ◽  
Lu Lu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sustained Release ◽  
Plasmid Dna ◽  
Nanofibrous Scaffold ◽  
Skin Substitute ◽  
Dna Encoding ◽  
Dermal Wound Healing ◽  
Plasmid Dnas ◽  
Dermal Wound

A gene-activated porous nanofibrous scaffold for effectively promoting vascularization, epidermalization and dermal wound healing by sustained release of dual plasmid DNAs.

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