scholarly journals Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 420
Author(s):  
Gyu Seong Yeom ◽  
In-ho Song ◽  
Shrikant Dashrath Warkad ◽  
Pramod B. Shinde ◽  
Taewoon Kim ◽  
...  

The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (ABIA) along with the design and development of a portable fluorescence spectrometer device (CysDDev) for detecting cysteine in simulated human urine. ABIA showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed CysDDev. Further, ABIA also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6352-6356 ◽  
Author(s):  
Narendra Reddy Chereddy ◽  
M. V. Niladri Raju ◽  
Peethani Nagaraju ◽  
Venkat Raghavan Krishnaswamy ◽  
Purna Sai Korrapati ◽  
...  

A naphthalimide based Fe3+ selective fluorescence ‘turn-on’ probe that operates based on a PET mechanism has been synthesized, and its application in the detection of Fe3+ ions in aqueous samples and in live cells is explored.


2012 ◽  
Vol 41 (24) ◽  
pp. 7212 ◽  
Author(s):  
Lin Xu ◽  
Yufang Xu ◽  
Weiping Zhu ◽  
Chunmei Yang ◽  
Le Han ◽  
...  

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3102 ◽  
Author(s):  
Bin Li ◽  
Datong Zhang ◽  
Ruibing An ◽  
Yaling Zhu

In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe (PBD) for the selective sensing of biothiols is reported. Upon treatment with biothiols, PBD shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to interference from other amino acids and competing species. PBD features good linearity ranges with a low detection limit of 14.5 nM for glutathione (GSH), 17.5 nM for cysteine (Cys), and 80.0 nM for homocysteine (Hcy), respectively. Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.


2015 ◽  
Vol 1119 ◽  
pp. 833-837
Author(s):  
Jia Jun Ma ◽  
Lei Cheng Yin ◽  
Gang Zou ◽  
Qi Jin Zhang

A simple fluorescent iodide ion sensor was fabricated. Ag+ was found to specifically induce the aggregation of perylene–3, 4, 9, 10–tetracarboxylate tetrapotassium salt (PTK) in water, simultaneously, fluorescence of PTK was quenched. The resultant PTK–Ag+ aggregates showed a "turn–on" fluorescence response for halide ion, especially I-, and excellent selectivity toward possible interfering anions, contain sulfide.


2018 ◽  
Vol 6 (4) ◽  
pp. 779-784 ◽  
Author(s):  
Nan Li ◽  
Aung Than ◽  
Jie Chen ◽  
Fengna Xi ◽  
Jiyang Liu ◽  
...  

Graphene quantum dots-based fluorescent turn-on nanoprobe is developed for real-time monitoring the triggered dynamic change of intracellular H2S.


2018 ◽  
Vol 42 (11) ◽  
pp. 8325-8329 ◽  
Author(s):  
An Xu ◽  
Yonghe Tang ◽  
Weiying Lin

The first mitochondrial-targeted two-photon fluorescence probe (MT-FA) for formaldehyde (FA) was engineered for monitoring FA in the mitochondria of living cells and liver tissues in one- and two-photon modes.


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