scholarly journals Detection of Salmonella Typhimurium in Romaine Lettuce Using a Surface Plasmon Resonance Biosensor

Biosensors ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 94 ◽  
Author(s):  
Devendra Bhandari ◽  
Fur-Chi Chen ◽  
Roger C. Bridgman
Keyword(s):  
Surface Plasmon Resonance ◽  
Salmonella Typhimurium ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Specificity ◽  
Leafy Vegetables ◽  
Detection Sensitivity ◽  
Sandwich Assay ◽  
Romaine Lettuce ◽  
Accurate Identification

Leafy vegetables have been associated with high-profile outbreaks causing severe illnesses. Timely and accurate identification of potential contamination is essential to ensure food safety. A surface plasmon resonance (SPR) assay has been developed for the detection of Salmonella Typhimurium in leafy vegetables. The assay utilizes a pair of well characterized monoclonal antibodies specific to the flagellin of S. Typhimurium. Samples of romaine lettuce contaminated with S. Typhimurium at different levels (between 0.9 and 5.9 log cfu/g) were pre-enriched in buffered peptone water. Three SPR assay formats, direct assay, sequential two-step sandwich assay, and pre-incubation one-step sandwich assay were evaluated. All three assay formats detect well even at a low level of contamination (0.9 log cfu/g). The SPR assay showed a high specificity for the detection of S. Typhimurium in the presence of other commensal bacteria in the romaine lettuce samples. The results also suggested that further purification of flagellin from the sample preparation using immunomagnetic separation did not improve the detection sensitivity of the SPR assay. The functional protocol developed in this study can be readily used for the detection of S. Typhimurium in leafy vegetables with high sensitivity and specificity.

scholarly journals Magnetic Nanoparticles Enhanced Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Romaine Lettuce

Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 475
Author(s):  
Devendra Bhandari ◽  
Fur-Chi Chen ◽  
Roger C. Bridgman
Keyword(s):  
Surface Plasmon Resonance ◽  
Magnetic Nanoparticles ◽  
Salmonella Typhimurium ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Rapid Detection ◽  
Sensor Surface ◽  
Sandwich Assay ◽  
Romaine Lettuce ◽  
One Step

Salmonella is one of the major foodborne pathogens responsible for many cases of illnesses, hospitalizations and deaths worldwide. Although different methods are available to timely detect Salmonella in foods, surface plasmon resonance (SPR) has the benefit of real-time detection with a high sensitivity and specificity. The purpose of this study was to develop an SPR method in conjunction with magnetic nanoparticles (MNPs) for the rapid detection of Salmonella Typhimurium. The assay utilizes a pair of well-characterized, flagellin-specific monoclonal antibodies; one is immobilized on the sensor surface and the other is coupled to the MNPs. Samples of romaine lettuce contaminated with Salmonella Typhimurium were washed with deionized water, and bacterial cells were captured on a filter membrane by vacuum filtration. SPR assays were compared in three different formats—direct assay, sequential two-step sandwich assay, and preincubation one-step sandwich assay. The interaction of flagellin and MNPs with the antibody-immobilized sensor surface were analyzed. SPR signals from a sequential two-step sandwich assay and preincubation one-step sandwich assay were 7.5 times and 14.0 times higher than the direct assay. The detection limits of the assay were 4.7 log cfu/mL in the buffer and 5.2 log cfu/g in romaine lettuce samples.

scholarly journals Kajian Pengaruh Material Graphene pada kinerja Biosensor Berbasis Surface Plasmon Resonance (SPR) pada Deteksi Makanan Halal sebagai Pendukung Halal Research Center UIN Sunan Kalijaga Yogyakarta

2017 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Wida Yanti ◽  
Asih Melati
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Analytical Calculation ◽  
Detection Sensitivity ◽  
Research Center ◽  
Attenuated Total Reflectance ◽  
Muslim Community ◽  
Spr Biosensor

<p><br />Halal foods and medicines are an absolute daily needs for the Muslim community in Indonesia. Therefore the authority institutions in indonesian goverment should ensure the availability of this. It is of course inseparable from the role of higher education through the development of its technology to develop halal detection of foods and drugs. This study is an effort to contribute to the Halal Research Center of UIN Sunan Kalijaga Yogyakarta through the biosensor development in halal detection foods and medicines based on biosensor SPR. This device using graphene materials to improve the detection sensitivity of pork gelatin material that is likely contained in foodstuffs and medicine. From analytical calculation and computation, enhancement of the SPR biosensor performance by involvement graphene it was shown through the ATR (Attenuated Total Reflectance) reflective curve. The result of this results was found the enhancement of the sensitivity 2,86 %.</p><p>Keyword: Surface Plasmon Resonance (SPR), Porcine Gelatin, Graphene, ATR</p>

scholarly journals Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

Nanophotonics ◽  
2017 ◽  
Vol 6 (5) ◽  
pp. 1017-1030 ◽  
Author(s):  
Youjun Zeng ◽  
Rui Hu ◽  
Lei Wang ◽  
Dayong Gu ◽  
Jianan He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Imaging Techniques ◽  
Detection Sensitivity ◽  
Label Free ◽  
Spr Biosensor ◽  
Recent Advances ◽  
Wavelength Filter ◽  
Detection Speed

AbstractSurface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

Hepatocellular Carcinoma Biomarker Detection by Surface Plasmon Resonance Sensor

2015 ◽  
Vol 1131 ◽  
pp. 84-87 ◽  
Author(s):  
Thidarat Wangkam ◽  
Kitti Boonperm ◽  
Phatra Khomkrachang ◽  
Toemsak Srikhirin ◽  
Verayuth Praphanphoj ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Binding Capacity ◽  
Abdominal Ultrasound ◽  
Optical Biosensor ◽  
Sandwich Assay ◽  
Highly Sensitive ◽  
Resonance Sensor

Hepatocellular carcinoma is a one of the most common cancer in the world and the third cause of cancer-related death. The recommended screening test for patients are the detection of serum α-fetoprotein (AFP) levels and an abdominal ultrasound every 6 months to detect HCC. To inform stage of the diasease, we investigate the alpha fetoprotein (AFP) which is a major HCC biomarker by using surface Plasmon resonance (SPR) technique. SPR is an optical biosensor, nanoscale detectable and highly sensitive technique which is suitable for an early detection application. The interaction of anti-AFP antibody and AFP antigen was created via sandwich assay on carboxy dextran sensor surface. To get the enhancing signal, effect of electrostatics and binding capacity of immobilization were studied. Additionally, we found that the higher enhancing signal is done by superimposing of anti-IgG on the system. The result was shown that AFP concentration was measured in a range of the HCC biomarker with the detection limit of 50 ng/ml and 20 ng/ml by sandwich assay and an anti-IgG enhancement, respectively. Moreover, the serum testing was studied for deveopment in clinical diagnostic further.

Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium

2004 ◽  
Vol 19 (11) ◽  
pp. 1497-1504 ◽  
Author(s):  
Byung-Keun Oh ◽  
Young-Kee Kim ◽  
Kwang Won Park ◽  
Won Hong Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Surface Plasmon Resonance ◽  
Salmonella Typhimurium ◽  
Surface Plasmon ◽  
Plasmon Resonance
Sign in / Sign up

Export Citation Format

Share Document