scholarly journals Drought Resistance Loci in Recombinant Lines of Iranian Oryza sativa L. in Germination Stage

BioTech ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 26
Author(s):  
Morteza Noryan ◽  
Islam Majidi Hervan ◽  
Hossein Sabouri ◽  
Faroukh Darvish Kojouri ◽  
Andrea Mastinu

In order to locate control genes related to Oryza sativa L. traits at the germination stage under normal conditions and at drought stress levels (−4.5 and −9.0 bar), we evaluated 120 F8 generation offspring from the cross between two cultivars Neda × Ahlemitarum in a factorial experiment in a completely randomized block design with three replications in 2013 in the botanical laboratory of Gonbad Kavous University. A linkage map was prepared using 90 Simple Sequence Repeats (SSR) markers and 28 Inter Simple Sequence Repeats (ISSR), and 6 iPBS and 9 IRAP markers (265 polymorphic alleles). The results of the analysis of variance showed that all of the evaluated traits had a significant difference at the probability level of 1%. Hence, it can be noted that the desired genetic diversity can be found between genotypes. The results of the stepwise regression analysis for the germination percentage as a dependent variable and other traits as independent variables in the studied treatments showed that under normal conditions, there was variable coleoptile length, but under drought stress of −4.5 and −9.0 bar, the variable plumule dry weight entered the model. In this study, the markers included in RM1-RM490 and ISSR2-3-RM133 of chromosomes 1 and 6 of Oryza sativa were identified as the main regulators of traits associated with Oryza sativa drought resistance. In particular, they present the quantitative trait loci (QTL) that control the first stages of germination of Oryza sativa in water stress conditions.

EUGENIA ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Vikson J. Porong

ABSTRACT   This study used a randomized block design with five standard treatments as follows: A1 (15 days), A2 (20 days), A3 (25 days), A4 (30 days), A5 (35 days). The treatment was repeated three times. The purpose of this study was to obtain the best age of the seeds in order to obtain high growth and production. The results of statistical analysis showed that there was no siginifcant difference in the number of unproductive tillers 70 days after transplanting, but this was not the case for the leaf area index; 70 days after germination there was a significant difference. A1 treatment (15 days) had the highest LAI is 4, 56. The same result was shown in the weight of 1000 grains of rice where there was a significant difference among treatments; A1 treatment (15 days) weighted 31.12. It was concluded that the age of seedlings after transplanting had a significant effect on LAI and 1000 grain weight of rice grain after harvest. Keywords: Rice, seedling, transplanting ABSTRACT   Penelitian ini menggunakan rancangan acak kelompok dengan lima taraf perlakuan sebagai berikut : A1 (15 hari), A2 (20 hari), A3 (25 hari), A4 (30 hari), A5 (35 hari). Perlakuan diulang sebanyak tiga kali. Tujuan penelitian ini untuk mendapatkan umur bibit yang tepat sehingga diperoleh pertumbuhan yang tinggi. Hasil analisis statistika menunjukkan bahwa tidak terdapat perbedaan yang nyata pada jumlah anakan yang tidak produktif 70 hari setelah pindah tanam namun pada indeks luas daun 70 hari setelah berkecambah terdapat suatu perbedaan yang nyata, terlihat pada perlakuan A1 (15 hari) memiliki ILD tertinggi yaitu 4,56. Hal yang sama terlihat pada bobot gabah 1000 butir terdapat suatu perbedaan yang nyata antara perlakuan yaitu pada perlakuan A1 (15 hari) memiliki bobot tinggi yaitu 31,12. Disimpulkan bahwa umur bibit setelah pindah tanam sangat berpengaruh pada ILD dan bobot gabah 1000 butir padi setelah panen. Kata kunci: Padi, bibit, pindah tanam


2013 ◽  
Vol 46 (3) ◽  
pp. 21-28 ◽  
Author(s):  
A. Ahmad ◽  
M. Afzal ◽  
A.U.H. Ahmad ◽  
M. Tahir

Abstract A field experiment was conducted at University of Agriculture, Faisalabad, Pakistan, to investigate the effect of foliar application of silicon on yield and quality of fine rice (Oryza sativa L.). The research was designed as randomized complete block design (RCBD) having three replications and 6m x 4.5m net plot size was maintained. Foliar applications of silicon’s aqueous solution were used as treatments comprised of control, 0.25%, 0.50%, 1.00% silicon solutions. Nursery of 30 days old seedling nursery was transplanted to the plots under aerobic condition and 22.5cm hill to hill distance was maintained. Sodium silicate (20.35% Si) as the source of silicon (soluble in warm water) was used. Fertilizer inputs as nitrogen, phosphorus and potassium were uniformly applied at the rate of 100, 67, 67 kg ha-1 while all other agronomic practices were kept constant for all the treatments. The data from the field (yield components) as well as lab analysis (quality parameters) was recorded according to the standard procedures. Fisher’s analysis of the variance technique was used for statistical analysis and treatment’s mean differences were compared using least significant difference (LSD) test at 5% probability level. Silicon showed no significant effect on plant height, harvest index, number of kernels and opaque kernels percentage. Silicon (0.50% silicon solution) produced maximum grain diameter and grain protein while silicon @ 1.00% silicon solution resulted maximum in number of productive tillers, straw yield, spike per panicle, 1000 grain weight, paddy yield and grain starch. All others parameters have overlapping results of different silicon levels.


2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Asma Asma ◽  
Iqbal Hussain ◽  
Muhammad Yasin Ashraf ◽  
Muhammad Arslan Ashraf ◽  
Rizwan Rasheed ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 643
Author(s):  
Mst. Motmainna ◽  
Abdul Shukor Juraimi ◽  
Md. Kamal Uddin ◽  
Norhayu Binti Asib ◽  
A. K. M. Mominul Islam ◽  
...  

Natural product-based herbicides could be the effective alternatives to synthetic chemical herbicides for eco-friendly weed management. This research, therefore, was conducted to identify the phytotoxic properties of Parthenium hysterophorus L., Cleome rutidosperma DC. and Borreria alata (Aubl.) DC. with a view to introducing them as a tool for natural herbicide development. The methanol extracts of these plants were examined on the germination and growth of Zea mays L., Oryza sativa L., Abelmoschus esculentus (L.) Moench and Amaranthus gangeticus L., Oryza sativa f. Spontanea Roshev. (Weedy rice), Echinochloa colona (L.) Link., Euphorbia hirta L., and Ageratum conyzoides L. under laboratory and glasshouse conditions. A complete randomized design (CRD) with five replications and randomized complete block design (RCBD) with four replications were laid out for laboratory and glasshouse experiments, respectively. In the laboratory experiment, three plant extracts of 0, 6.25, 12.5, 50, and 100 g L−1 were tested on survival rate, hypocotyl, and radicle length of eight test plant species. No seed germination of A. conzyoides, E. hirta, and A. gangeticus were recorded when P. hysterophorus extract was applied at 50 g L−1. C. rutidosperma had the same effect on those plants at 100 g L−1. In the glasshouse, similar extracts and concentrations used in the laboratory experiments were sprayed on at the 2–3 leaf stage for grasses and 4–6 for the broadleaf species. Tested plants were less sensitive to C. rutidosperma and B. alata compared to P. hysterophorus extract. Among the weeds and crops, A. conyzoides, E. hirta, A. esculentus and A. gangeticus were mostly inhibited by P. hysterophorus extract at 100 g L−1. Based on these results, P. hysterophorus was the most phytotoxic among the tested plant extracts and could be used for developing a new natural herbicide for green agriculture.


2012 ◽  
Vol 39 (5) ◽  
pp. 402 ◽  
Author(s):  
Veeresh R. P. Gowda ◽  
Amelia Henry ◽  
Vincent Vadez ◽  
H. E. Shashidhar ◽  
Rachid Serraj

In addition to characterising root architecture, evaluating root water uptake ability is important for understanding drought response. A series of three lysimeter studies were conducted using the OryzaSNP panel, which consists of 20 diverse rice (Oryza sativa L.) genotypes. Large genotypic differences in drought response were observed in this genotype panel in terms of plant growth and water uptake. Total water uptake and daily water uptake rates in the drought-stress treatment were correlated with root length density, especially at depths below 30 cm. Patterns of water uptake among genotypes remained consistent throughout the stress treatments: genotypes that initially extracted more water were the same genotypes that extracted more water at the end of the study. These results suggest that response to drought by deep root growth, rather than a conservative soil water pattern, seems to be important for lowland rice. Genotypes in the O. sativa type aus group showed some of the greatest water uptake and root growth values. Since the OryzaSNP panel has been genotyped in detail with SNP markers, we expect that these results will be useful for understanding the genetics of rice root growth and function for water uptake in response to drought.


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