scholarly journals Characterization of RIN Isoforms and Their Expression in Tomato Fruit Ripening

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1739
Author(s):  
Maria A. Slugina ◽  
Gleb I. Efremov ◽  
Anna V. Shchennikova ◽  
Elena Z. Kochieva

Ripening of tomato fleshy fruit is coordinated by transcription factor RIN, which triggers ethylene and carotenoid biosynthesis, sugar accumulation, and cell wall modifications. In this study, we identified and characterized complete sequences of the RIN chromosomal locus in two tomato Solanum lycopersicum cultivars, its rin/RIN genotype, and three wild green-fruited species differing in fruit color and composition. The results reveal that S. lycopersicum cultivars and some wild species (S. pennellii, S. habrochaites, and S. huaylasense) had a 3′-splicing site enabling the transcription of RIN1i and RIN2i isoforms. The other wild species (S. arcanum, S. chmielewskii, S. neorickii, and S. peruvianum) had a 3′-splicing site only for RIN2i, which was consistent with RIN1i and RIN2i expression patterns. The genotype rin/RIN, which had an extended 3′-terminal deletion in the rin allele, mainly expressed the chimeric RIN–MC transcript, which was also found in cultivars (RIN/RIN). The RIN1, but not RIN2, protein is able to induce the transcription of the reporter gene in the Y2H system, which positively correlated with the transcription profile of RIN1i and RIN target genes. We suggest that during fruit ripening, RIN1 activates ripening-related genes, whereas RIN2 and RIN–MC act as modulators by competing for RIN-binding sites in gene promoters, which should be confirmed by further studies on the association between RIN-splicing mechanisms and tomato fruit ripening.

2016 ◽  
Vol 97 (3) ◽  
pp. 991-1000 ◽  
Author(s):  
Ling Li ◽  
Xiaoguang Wang ◽  
Xinhua Zhang ◽  
Mei Guo ◽  
Tieling Liu

1987 ◽  
Vol 84 (3) ◽  
pp. 911-917 ◽  
Author(s):  
Birgit Piechulla ◽  
Richard E. Glick ◽  
Hubert Bahl ◽  
Anastasios Melis ◽  
Wilhelm Gruissem

2009 ◽  
Vol 103 (1) ◽  
pp. 116-119 ◽  
Author(s):  
G.G. Romero ◽  
C.C. Martinez ◽  
E.E. Alanís ◽  
G.A. Salazar ◽  
V.G. Broglia ◽  
...  

2019 ◽  
Vol 10 ◽  
Author(s):  
Stefan Petrasch ◽  
Christian J. Silva ◽  
Saskia D. Mesquida-Pesci ◽  
Karina Gallegos ◽  
Casper van den Abeele ◽  
...  

2018 ◽  
Vol 143 (6) ◽  
pp. 454-461 ◽  
Author(s):  
Xiaohong Wang ◽  
Bishun Ye ◽  
Xiangpeng Kang ◽  
Ting Zhou ◽  
Tongfei Lai

Tomato (Solanum lycopersicum) fruit ripening is a complex genetic trait correlating with notable fruit phenotypic, physiologic, and biochemical changes. Transcription factors (TFs) play crucial roles during this process. LeHB-1, an HD-zip homeobox protein, is a ripening-related TF and acts as an important regulator of fruit ripening. However, the detailed biochemical and molecular basis of LeHB-1 on tomato fruit ripening is unclear. In the current study, the biologic functions of LeHB-1 were determined by a potato virus X (PVX)-mediated gene-silencing approach. The results indicate that PVX-induced LeHB-1 silencing in tomato could decrease pigment accumulation and delay fruit ripening. Compared with controls, nonripening flesh retains a greater pH value and a lesser anthocyanin content. By evaluating expression levels of genes related to tomato fruit ripening, we inferred that LeHB-1 located at the downstream of LeMADS-RIN-mediated regulatory network. In addition, LeHB-1 silencing mainly disturbed phytoene desaturation and isomerization, and led to a decrease in trans-lycopene accumulation, but did not influence flavonoid biosynthesis directly in tomato fruit. The findings provide a theoretical foundation for illustrating the biologic functions of LeHB-1 in tomato fruit ripening and quality.


2018 ◽  
Vol 136 ◽  
pp. 90-98 ◽  
Author(s):  
Junliang Yin ◽  
Mengyu Liu ◽  
Dongfang Ma ◽  
Jiawen Wu ◽  
Shenglan Li ◽  
...  

2020 ◽  
Vol 71 (12) ◽  
pp. 3759-3759
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Zhongqi Fan ◽  
Xiaodan Zhao ◽  
Yiping Zhang ◽  
...  

2019 ◽  
Vol 70 (10) ◽  
pp. 2727-2740 ◽  
Author(s):  
Xuemin Ma ◽  
Salma Balazadeh ◽  
Bernd Mueller-Roeber

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