scholarly journals Quantitative Trait Locus Mapping of Seed Vigor in Soybean under −20 °C Storage and Accelerated Aging Conditions via RAD Sequencing

2021 ◽  
Vol 43 (3) ◽  
pp. 1977-1996
Author(s):  
Rongfan Wang ◽  
Fengqi Wu ◽  
Xianrong Xie ◽  
Cunyi Yang

Due to its fast deterioration, soybean (Glycine max L.) has an inherently poor seed vigor. Vigor loss occurring during storage is one of the main obstacles to soybean production in the tropics. To analyze the genetic background of seed vigor, soybean seeds of a recombinant inbred line (RIL) population derived from the cross between Zhonghuang24 (ZH24, low vigor cultivar) and Huaxia3hao (HX3, vigorous cultivar) were utilized to identify the quantitative trait loci (QTLs) underlying the seed vigor under −20 °C conservation and accelerated aging conditions. According to the linkage analysis, multiple seed vigor-related QTLs were identified under both −20 °C and accelerated aging storage. Two major QTLs and eight QTL hotspots localized on chromosomes 3, 6, 9, 11, 15, 16, 17, and 19 were detected that were associated with seed vigor across two storage conditions. The indicators of seed vigor did not correlate well between the two aging treatments, and no common QTLs were detected in RIL populations stored in two conditions. These results indicated that deterioration under accelerated aging conditions was not reflective of natural aging at −20 °C. Additionally, we suggest 15 promising candidate genes that could possibly determine the seed vigor in soybeans, which would help explore the mechanisms responsible for maintaining high seed vigor.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 479b-479
Author(s):  
Anwar A. Khan

1-aminocyclopropane-1-carboxylic acid (ACC) was used to measure seed vigor of lettuce, cabbage, tomato, snap bean and sweet corn seeds. Accelerated aging at 40C and 93% RH over saturated solution of KH2PO4 and natural aging under ambient storage conditions (5-7C, 28 to 60% RH) were used to obtain seeds of differing vigor levels. Depending on the type of seeds, the amount of ACC needed for maximal ethylene production (saturating dose) ranged from 0.25 to 2mM. Seeds produced much larger amounts of ethylene in the presence than in absence of ACC, the ACC-derived ethylene was detected prior to germination, and ACC had no adverse effect on germination. ACC-derived ethylene production paralleled vigor loss as determined by percentage germination, mean germination time (for lettuce only) and seedling growth (for snap bean only). Second degree polynomial and logarithmic equations generated for the relationship of ACC-derived ethylene production to germination or growth parameters following seed aging, provided good to excellent fit. As a vigor test, the ACC-ethylene procedure has several advantages over the non-ACC ethylene procedure: it greatly improves the sensitivity of the test by enhancing ethylene production, it permits detection of small differences in vigor levels and it allows detection of ethylene prior to germination within a few hours of soaking.


2018 ◽  
Vol 294 (1) ◽  
pp. 243-252 ◽  
Author(s):  
Tatsuhiko Goto ◽  
Akira Ishikawa ◽  
Masahide Nishibori ◽  
Masaoki Tsudzuki

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