scholarly journals Serum Metabolomics Analysis for Biomarkers of Lactobacillus plantarum FRT4 in High-Fat Diet-Induced Obese Mice

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 184
Author(s):  
Hongying Cai ◽  
Zhiguo Wen ◽  
Xin Xu ◽  
Jiaxin Wang ◽  
Xuan Li ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
High Fat Diet ◽  
Ultra Performance Liquid Chromatography ◽  
Leukotriene B4 ◽  
Arachidonic Acid Metabolism ◽  
High Fat ◽  
Flight Mass Spectrometry ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis ◽  
Serum Metabolomics

Lactobacillus plantarum is considered a potential probiotic supplementation for treating obesity. However, the underlying molecular mechanism is poorly understood. Our previous study displayed that L. plantarum FRT4 alleviated obesity in mice fed a high-fat diet (HFD) through ameliorating the HFD-induced gut microbiota dysbiosis. To explore the roles of FRT4 in obesity prevention, in this study, we investigated changes in serum metabolomic phenotype by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and analyzed the pathway of HFD-fed Kunming female mice orally administered with FRT4 for eight weeks. Using orthogonal partial least squares discriminant analysis (OPLS-DA), metabolite patterns with significant changes were observed. 55 metabolites including phosphatidylcholine, lysophophatidylcholine, sphingomyelin, serotonin, indole-3-methyl aceta, indole-3-carbinol, indole-5,6-quino, 11,12-DHET, prostaglandin B2, leukotriene B4, and 3-hydroxybenzoic acid were identified as potential biomarkers associated with obesity, which were mainly involving in glycerophospholipid metabolism, tryptophan metabolism, and arachidonic acid metabolism. Perturbations of 14 biomarkers could be regulated by FRT4 intervention. These metabolites may serve as valuable biomarkers to understand the mechanisms by which intake of diets containing FRT4 contributes to the treatment or prevention of obesity. Thus, FRT4 can be a promising dietary supplement for the prevention of HFD-induced obesity.

scholarly journals Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 733
Author(s):  
Francisco Javier Leyva-Jiménez ◽  
Antonio Jesús Ruiz-Malagón ◽  
José Alberto Molina-Tijeras ◽  
Patricia Diez-Echave ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Fat Diet ◽  
High Fat ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Anti Inflammatory ◽  
Tof Ms

Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of different genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH• radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf-α, Il-1β, Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory effects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory effect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders.

Lactobacillus plantarum KFY04 prevents obesity in mice through the PPAR pathway and alleviates oxidative damage and inflammation

2020 ◽  
Vol 11 (6) ◽  
pp. 5460-5472
Author(s):  
Xingyao Long ◽  
Xiaofei Zeng ◽  
Fang Tan ◽  
Ruokun Yi ◽  
Yanni Pan ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Oxidative Damage ◽  
Lactobacillus Plantarum ◽  
High Fat Diet ◽  
Adipocyte Size ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Ppar Pathway

Lactobacillus plantarum KFY04 can significantly inhibited the increase of adipocytes and decrease the adipocyte size in adipose tissue. LP-KFY04 can be used to mitigate high-fat-diet-induced obesity in mice.

Regulating effect of Lactobacillus plantarum CQPC03 on lipid metabolism in high‐fat diet‐induced obesity in mice

2020 ◽  
Vol 44 (11) ◽  
Author(s):  
Yi Gan ◽  
Hong Chen ◽  
Xian‐Rong Zhou ◽  
Ling‐Ling Chu ◽  
Wan‐Ting Ran ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Lactobacillus Plantarum ◽  
High Fat Diet ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice

scholarly journals Lactobacillus fermentum CECT5716 ameliorates high fat diet-induced obesity in mice through modulation of gut microbiota dysbiosis

2021 ◽  
pp. 105471
Author(s):  
Jose Alberto Molina-Tijeras ◽  
Patricia Diez-Echave ◽  
Teresa Vezza ◽  
Laura Hidalgo-García ◽  
Antonio Jesús Ruiz-Malagón ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis

scholarly journals Lactobacillus fermentum CECT5716 ameliorates high fat diet-induced obesity in mice through modulation of gut microbiota dysbiosis.

2020 ◽  
Author(s):  
Jose Alberto Molina Tijeras ◽  
Patricia Diez Echave ◽  
Teresa Vezza ◽  
Laura Hidalgo Garcia ◽  
Antonio Jes s Ruiz Malag n ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Lactobacillus Fermentum ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis

scholarly journals Eurotium cristatum, a new fungi probiotic from Fuzhuan brick tea, alleviated obesity in mice by modulating gut microbiota

2019 ◽  
Author(s):  
Dingding Kang ◽  
Meng Su ◽  
Yanwen Duan ◽  
Yong Huang
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Microbiota Composition ◽  
High Fat ◽  
Daily Consumption ◽  
Fecal Transplantation ◽  
Experimental Animals ◽  
Obesity In Mice ◽  
And Storage ◽  
Gut Microbiota Dysbiosis

AbstractObesity is one of the major public health problems worldwide, mainly resulting from unhealthy lifestyles and diet. Gut microbiota dysbiosis may also lead to obese humans and animals. Modulating gut bacteria through fecal transplantation, the use of probiotics or certain dietary supplements, could normalize gut microbiota and subsequently alleviate obesity. Daily consumption of Fuzhuan brick tea (FBT) or its extracts have been observed to alleviate obesity in humans and experimental animals. In this study, high-fat diet-induced dysbiosis of gut microbiota in C57BL/6J mice was partially reversed by consumption of Eumtium cristatum, the dominant fungi during the manufacturing and storage of FBT. E. cristatum was able to modulate both gut fungi and bacteria composition, based on the analysis of microbiota composition of mice fecal samples. E. cristatum increased acetate and butyrate-producing bacteria in mice gut, and produced five times more butyrate than both obese and normal mice. Our results suggested that E. cristatum may be used as a fungi probiotic to beneficially modulate gut microbiota and to alleviate obesity in humans.

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