scholarly journals A Comparative Analysis of Lipid Digestion in Human Milk and Infant Formulas Based on Simulated In Vitro Infant Gastrointestinal Digestion

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 200
Author(s):  
Lu Liu ◽  
Shuang Lin ◽  
Shuaiyi Ma ◽  
Yue Sun ◽  
Xiaodong Li ◽  
...  

To investigate the lipid digestive behaviors of human and infant formulas and analyze the differences between them, we investigated the fat globule particle size distribution, lipolysis rate, and fatty acid release of infant formulas with different fat sources and human milk using an in vitro infant digestion model. The results suggested that the particle size in infant formula increased rapidly during gastric digestion and decreased significantly after intestinal digestion, whereas the particle size in human milk increased slowly during gastric digestion but increased rapidly during intestinal digestion (p < 0.05). Despite having a larger droplet size, human milk demonstrated a very high lipolysis rate due to the presence of MFGM. In terms of the distribution of fatty acids in digestion products, the proportion of saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) in vegetable oil-based infant formulas was close to that of human milk. The amount of SFAs in milk fat-based infant formulas was significantly higher than that in human milk, and the content of MUFAs in all infant formulas was significantly lower than that in human milk (p < 0.05). After digestion, the most abundant fatty acid released by human milk was C18:2n6c, while the fatty acids released by infant formulas were SFAs, such as C14:0, C16:0, and C18:0.

2019 ◽  
Vol 10 (4) ◽  
pp. 2102-2113 ◽  
Author(s):  
Jeske H. J. Hageman ◽  
Jaap Keijer ◽  
Trine Kastrup Dalsgaard ◽  
Lara W. Zeper ◽  
Frédéric Carrière ◽  
...  

The profile of fatty acids released during in vitro digestion of vegetable and bovine milk fat-based infant formula differ.


2019 ◽  
Vol 10 (5) ◽  
pp. 3018-3020
Author(s):  
Jeske H. J. Hageman ◽  
Jaap Keijer ◽  
Trine Kastrup Dalsgaard ◽  
Lara W. Zeper ◽  
Frédéric Carrière ◽  
...  

Correction for ‘Free fatty acid release from vegetable and bovine milk fat-based infant formulas and human milk during two-phase in vitro digestion’ by Jeske H. J. Hageman et al., Food Funct., 2019, 10, 2102–2113.


Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1972 ◽  
Author(s):  
Wesolowska ◽  
Brys ◽  
Barbarska ◽  
Strom ◽  
Szymanska-Majchrzak ◽  
...  

Human milk fat plays an essential role as the source of energy and cell function regulator; therefore, the preservation of unique human milk donors’ lipid composition is of fundamental importance. To compare the effects of high pressure processing (HPP) and holder pasteurization on lipidome, human milk was processed at 62.5 °C for 30 min and at five variants of HPP from 450 MPa to 600 MPa, respectively. Lipase activity was estimated with QuantiChrom™ assay. Fatty acid composition was determined with the gas chromatographic technique, and free fatty acids content by titration with 0.1 M KOH. The positional distribution of fatty acid in triacylglycerols was performed. The oxidative induction time was obtained from the pressure differential scanning calorimetry. Carotenoids in human milk were measured by liquid chromatography. Bile salt stimulated lipase was completely eliminated by holder pasteurization, decreased at 600 MPa, and remained intact at 200 + 400 MPa; 450 MPa. The fatty acid composition and structure of human milk fat triacylglycerols were unchanged. The lipids of human milk after holder pasteurization had the lowest content of free fatty acids and the shortest induction time compared with samples after HPP. HPP slightly changed the β-carotene and lycopene levels, whereas the lutein level was decreased by 40.0% up to 60.2%, compared with 15.8% after the holder pasteurization.


2019 ◽  
Vol 10 (8) ◽  
pp. 5174-5187 ◽  
Author(s):  
Clay Swackhamer ◽  
Zhichao Zhang ◽  
Ameer Y. Taha ◽  
Gail M. Bornhorst

In vitro gastric digestion of almond particles using a model with simulated peristaltic contractions resulted in particle size reduction and higher fatty acid bioaccessibility than in vitro digestion using a model that lacked peristaltic contractions.


1963 ◽  
Vol 9 (5) ◽  
pp. 530-543 ◽  
Author(s):  
Bernard J Katchman ◽  
Robert E Zipf ◽  
James P F Murphy

Abstract The kinetic effect of palmitate, stearate, oleate, linoleate, and linolenate upon in vitro endogenous respiration of rat chloromyeloid leukemic cells has been investigated. Inhibition of respiration has been correlated with the ability of fatty acids to cause decreased cell viability and cell count; in the bioassay of fatty acid-treated tumor inocula, the increase in animal life span is correlated to the degree of dilution of the inocula due to cell lysis. The degree of lysis is dependent upon the chemical structure of the fatty acid, concentration, and duration of contact; unsaturated fatty acids are more effective than saturated fatty acids. Tumor cells, when incubated at low concentrations of fatty acids, show stimulation of O2 uptake; however, in the bioassay these fatty acid-treated inocula showed no loss in tumor activity. The nature of the physiochemical interaction between fatty acids and tumor cells is discussed.


2018 ◽  
Vol 3 (3) ◽  
pp. 12-26
Author(s):  
Marina I. Baburina ◽  
Natal’ya L. Vostrikova ◽  
Andrew N. Ivankin ◽  
Aleksandr N. Zenkin

The aspects of biochemical transformation of natural lipids of vegetable, fish, and animal origin are considered on the model of biotechnical aspects of triglyceride transformation into products of various purpose. The indicators of biological efficacy of fats are presented regarding the systematization by groups of polyunsaturated, monounsaturated and saturated fatty acids. Some features of simulation of natural processes of hydrolytic fat degradation into energy-intensive products are discussed. At the same time, aspects of fat intake and their biochemical transformation in food systems by human enzymes, and biochemical transformation of lipids in vitro in the presence of commercial enzyme preparations were studied. The aspects of free fatty acid transformation into esters are considered for justifying their use.


2000 ◽  
Vol 84 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Franca Marangoni ◽  
Carlo Agostoni ◽  
Anna M. Lammard ◽  
Marcello Giovannini ◽  
Claudio Galli ◽  
...  

While a wealth of data on the fatty acid composition of mature human milk has been published, limited information is available on the quantities of individual fatty acids supplied to the suckling infant with maternal milk, through the whole first year of life. Our aim was to qualitatively and quantitatively evaluate the fatty acid composition of human milk from Italian mothers, throughout extended lactation with particular emphasis on the long-chain polyunsaturated fatty acids. We have thus measured the total fat content and the concentrations of major fatty acids by quantitative GLC in pooled breast hindmilk collected from all feedings over 24 h at colostrum, 1, 3, 6, 9 and 12 months in ten mothers recruited after delivery of full-term infants. Total saturated fatty acids progressively increase and total monounsaturated progressively decrease as percentage levels, while among long-chain polyunsaturated fatty acids, percentages of arachidonic acid and docosahexaenoic acid decrease from colostrum up to the third month. Hindmilk total lipids (mg/dl) rise more than twofold up to 3 months, and then remain stable. The amounts (mg/dl) of linoleic acid and α-linolenic acid progressively increase, following the trend of total fat, while arachidonic and docosahexaenoic concentrations (mg/dl) remain stable throughout the whole nursing period. Assessment of the intakes per kg body weight shows different trends for the individual major long-chain polyunsaturated fatty acids supplied to the infant from hindmilk during exclusive breast-feeding (3 months). This information may be useful for the evaluation of infant intakes during extended lactation.


2003 ◽  
Vol 90 (5) ◽  
pp. 979-986 ◽  
Author(s):  
Leon R. Mitoulas ◽  
Lyle C. Gurrin ◽  
Dorota A. Doherty ◽  
Jillian L. Sherriff ◽  
Peter E. Hartmann

Despite the importance of human milk fatty acids for infant growth and development, there are few reports describing infant intakes of individual fatty acids. We have measured volume, fat content and fatty acid composition of milk from each breast at each feed over a 24h period to determine the mean daily amounts of each fatty acid delivered to the infant from breast milk at 1, 2, 4, 6, 9 and 12 months of lactation in five women. Daily (24h) milk production was 336·60 (sem 26·21) and 414·49 (sem 28·39) ml and milk fat content was 36·06 (sem 1·37) and 34·97 (sem 1·50) g/l for left and right breasts respectively over the course of the first year of lactation. Fatty acid composition varied over the course of the day (mean CV 14·3 (sd 7·7) %), but did not follow a circadian rhythm. The proportions (g/100g total fatty acids) of fatty acids differed significantly between mothers (P<0·05) and over the first year of lactation (P<0·05). However, amounts (g) of most fatty acids delivered to the infant over 24h did not differ during the first year of lactation and only the amounts of 18:3n-3, 22:5n-3 and 22:6n-3 delivered differed between mothers (P<0·05). Mean amounts of 18:2n-6, 18:3n-3, 20:4n-6 and 22:6n-3 delivered to the infant per 24h over the first year of lactation were 2·380 (sd 0·980), 0·194 (sd 0·074), 0·093 (sd 0·031) and 0·049 (sd 0·021) g respectively. These results suggest that variation in proportions of fatty acids may not translate to variation in the amount delivered and that milk production and fat content need to be considered.


2018 ◽  
Vol 85 (2) ◽  
pp. 257-262 ◽  
Author(s):  
Antonella Santillo ◽  
Lucia Figliola ◽  
Maria G Ciliberti ◽  
Mariangela Caroprese ◽  
Rosaria Marino ◽  
...  

We report the fatty acid profile of raw milk and of the corresponding digested milk from different sources (human milk, formula milk and donkey, bovine, ovine and caprine milk) to gain information on the nutritional quality of different milk sources in infant nutrition.Short chain fatty acids (SC-FA) were higher in bovine and caprine milk, intermediate in ovine and donkey and lower in human and formula milk. Medium chain fatty acids (MC-FA) showed the highest values for bovine and caprine milk and the lowest for donkey and formula milk, whereas long chain fatty acids (LC-FA) were the highest in donkey and formula milk and intermediate in human milk.The percentage distribution of fatty acids liberated after in vitro digestion did not reflect the patterns found in the corresponding milk sources. In particular, MC free fatty acids (MC-FFA) showed the highest and the lowest values in donkey and in formula milk, LC-FFA showed the highest value in human milk. The total FFA was highest in human milk, lowest in formula milk and intermediate in donkey, bovine, ovine, and caprine milk.


1968 ◽  
Vol 46 (3) ◽  
pp. 205-210 ◽  
Author(s):  
G. M. Moore ◽  
J. B. M. Rattray ◽  
D. M. Irvine

Lipid was extracted from lyophilized samples of cow, goat, sheep, horse, and human milk. The cephalins were isolated by thin-layer chromatography and ascertained to be the major, but variable component of all milk phospholipids examined except in the case of the horse. The predominant cephalin type was found to be phosphatidyl ethanolamine (PE), while phosphatidyl serine (PS) was determined to be either absent or to account for approximately 20% of the total cephalins. Lysocephalins were generally absent. The fatty acid composition of PE and PS was characterized by high levels of oleic and palmitic acids and by the virtual lack of long-chain polyunsaturated fatty acids. Individual differences in fatty acid content were slight among members of the same species, but human milk PE showed considerable variations in composition. PE was determined to be more highly unsaturated than PS with the unsaturated fatty acids occurring primarily at the β-position of the PE molecule. The results obtained on the chemical structure of milk cephalins have been discussed in relationship to the suggested activity of these compounds on the blood coagulation process in vitro.


Sign in / Sign up

Export Citation Format

Share Document