Development of Hydrogels with the Incorporation of Raphanus sativus L. Seed Extract in Sodium Alginate for Wound-Healing Application

Hydrogels prepared from polymers have been proposed for tissue regeneration and the treatment of bruise wounds. In this research work, we synthesized a Raphanus sativus L.-based wound-healing hydrogel with recognized antimicrobial activity for the healing of cutaneous lesions, drawing on its healing potential. A structural analysis was performed by Fourier transform infrared spectroscopy, confirming the interaction between sodium alginate and Raphanus sativus L. The surface morphology was studied by scanning electron microscopy. A swelling test showed that the T-1 hydrogel capability of absorption of the solution was superior compared to other synthesized samples. It was evident that the swelling tendency decreased as the Raphanus sativus L. seed extract concentration was reduced. In a thermogravimetric analysis, T-1 shows high thermal stability over other prepared hydrogel samples, enjoying a high content of seed extract compared with all samples. The prepared hydrogels were placed on the chick chorioallantoic membrane of fertilized chick eggs, and their healing capability was examined. All seed extracts containing hydrogels showed clear curative performance as compared to the control hydrogel, whereas their healing magnitude lessened as the extract ratio decreased. It was concluded from the results of the current study that the Raphanus sativus L. plant has wound-healing characteristics.

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Sustainable Development of Chitosan/Calotropis procera-Based Hydrogels to Stimulate Formation of Granulation Tissue and Angiogenesis in Wound Healing Applications

Molecules ◽

10.3390/molecules26113284 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3284

Author(s):

Muhammad Zahid ◽

Maria Lodhi ◽

Zulfiqar Ahmad Rehan ◽

Hamna Tayyab ◽

Talha Javed ◽

...

Keyword(s):

Wound Healing ◽

Blood Vessels ◽

Granulation Tissue ◽

Chorioallantoic Membrane ◽

Calotropis Procera ◽

Connective Tissues ◽

Blood Capillaries ◽

Chick Chorioallantoic Membrane ◽

Thaw Cycle ◽

Freeze Thaw Cycle

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

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Inhibition of Growth of Escherichia coli O157:H7 in Fresh Radish (Raphanus sativus L.) Sprout Production by Calcinated Calcium

Journal of Food Protection ◽

10.4315/0362-028x-62.2.128 ◽

1999 ◽

Vol 62 (2) ◽

pp. 128-132 ◽

Cited By ~ 41

Author(s):

M. L. BARI ◽

H. KUSUNOKI ◽

H. FURUKAWA ◽

H. IKEDA ◽

K. ISSHIKI ◽

...

Keyword(s):

Escherichia Coli ◽

Raphanus Sativus ◽

Inhibitory Effect ◽

Seed Extract ◽

Escherichia Coli O157 ◽

Experimental Conditions ◽

E Coli ◽

Inhibition Of Growth ◽

Raphanus Sativus L ◽

Radish Sprouts

The inhibitory effect of calcinated calcium on the growth of Escherichia coli O157:H7 during fresh radish (Raphanus sativus L.) sprout production was studied. It was revealed that the addition of 0.4% (wt/vol) calcinated calcium into radish sprouting medium which was artificially contaminated with E. coli O157:H7 (3.0 to 3.2 log CFU/ml) completely inhibited the growth or inactivated the microorganism. When radish seed extract was used instead of radish sprout production, the same extent of growth inhibition or inactivation was observed with much lower amounts (0.07%) of calcinated calcium under similar experimental conditions. The findings suggested that calcinated calcium may be useful to control E. coli O157:H7 contamination during the production of radish sprouts.

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Silver Nanoparticles-Composing Alginate/Gelatine Hydrogel Improves Wound Healing In Vivo

Nanomaterials ◽

10.3390/nano10020390 ◽

2020 ◽

Vol 10 (2) ◽

pp. 390 ◽

Cited By ~ 17

Author(s):

Flavia Resende Diniz ◽

Romerito Cesar A. P. Maia ◽

Lucas Rannier Andrade ◽

Luciana Nalone Andrade ◽

Marco Vinicius Chaud ◽

...

Keyword(s):

Wound Healing ◽

Silver Nanoparticles ◽

Sodium Alginate ◽

Time Course ◽

In Vivo Studies ◽

Natural Polymers ◽

Cutaneous Lesions ◽

Cutaneous Wounds ◽

Healing Tissue

Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430–450 nm in the ultraviolet-visible (UV–Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT–IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm−1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.

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THYME OIL LOADED CASSAVA STARCH TRANSDERMAL FILM FOR WOUND HEALING

INDIAN DRUGS ◽

10.53879/id.58.02.12004 ◽

2021 ◽

Vol 58 (02) ◽

pp. 76-81

Author(s):

Vinita Patole ◽

Rajnigandha Gaikwad ◽

Kishanchandra Khandelwal ◽

Keyword(s):

Wound Healing ◽

Chorioallantoic Membrane ◽

Antimicrobial Properties ◽

Volatile Oil ◽

Cassava Starch ◽

Vapour Permeability ◽

Angiogenic Potential ◽

Thyme Oil ◽

Chick Chorioallantoic Membrane ◽

Transdermal Film

Biodegradable cassava starch-based transdermal film loaded with thyme volatile oil was prepared by solvent casting method for wound healing activity. The stretchability, water vapour permeability and antimicrobial properties of the film were measured. The films were evaluated for drug-excipient compatibility studies by Fourier transformed infrared spectroscopy (FTIR). The formulated film loaded with thyme volatile oil exhibited good anti-microbial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The film was tested to determine its potential to increase the number of capillaries on the treated chick chorioallantoic membrane (CAM) surfaces using nine days old fertilized chick eggs. These thyme oil films loaded with cassava starch displayed angiogenic potential, which is required in the treatment of wound healing.

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In VitroEvaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds

BioMed Research International ◽

10.1155/2015/108571 ◽

2015 ◽

Vol 2015 ◽

pp. 1-14 ◽

Cited By ~ 25

Author(s):

Elizabeth A. Wahl ◽

Fernando A. Fierro ◽

Thomas R. Peavy ◽

Ursula Hopfner ◽

Julian F. Dye ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Three Dimensional ◽

Chorioallantoic Membrane ◽

Angiogenic Potential ◽

Chick Chorioallantoic Membrane ◽

Bovine Collagen

Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis.In vitroresults demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCsin vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.

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