scholarly journals A Study on the Skin Whitening Activity of Digesta from Edible Bird’s Nest: A Mucin Glycoprotein

Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 24
Author(s):  
Qunyan Fan ◽  
Jianmei Lian ◽  
Xuncai Liu ◽  
Fengyang Zou ◽  
Xin Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sialic Acid ◽  
Tyrosinase Activity ◽  
Contribution Rate ◽  
Skin Whitening ◽  
Cell Experiment ◽  
Mucin Glycoprotein ◽  
Edible Bird’S Nest ◽  
Whitening Activity ◽  
Free Sialic Acid

Edible bird’s nest (EBN) is an unusual mucin glycoprotein. In China, it is popular among consumers due to its skin whitening activity. However, the relationship between protein, sialic acid, and the whitening activity of EBN after digestion is still unclear. In the present work, the whitening activity (antioxidant activity and tyrosinase inhibitory activity) of digested EBN were studied by HepG2 and B16 cell models. The dissolution rate of protein and sialic acid was 49.59% and 46.45% after the simulated digestion, respectively. The contents of free sialic acid and glycan sialic acid in EBN digesta were 17.82% and 12.24%, respectively. HepG2 cell experiment showed that the digested EBN had significant antioxidant activity, with EC50 of 1.84 mg/mL, and had a protective effect on H2O2-induced oxidative damage cells. The results of H2O2-induced oxidative damage showed that the cell survival rate increased from 40% to 57.37% when the concentration of digested EBN was 1 mg/mL. The results of the B16 cell experiment showed that the digested EBN had a significant inhibitory effect on tyrosinase activity, and the EC50 value of tyrosinase activity was 7.22 mg/mL. Cell experiments showed that free sialic acid had stronger antioxidant activity and tyrosinase inhibitory activity than glycan sialic acid. The contribution rate analysis showed that protein component was the main antioxidant component in digestive products, and the contribution rate was 85.87%; free sialic acid was the main component that inhibited tyrosinase activity, accounting for 63.43%. The products of the complete digestion of EBN are suitable for the development of a new generation of whitening health products.

Free Sialic Acid Storage Disease Mimicking Cerebral Palsy and Revealed by Blood Smear Examination

2011 ◽  
Vol 158 (1) ◽  
pp. 165-165.e1 ◽  
Author(s):  
François-Guillaume Debray ◽  
Caroline Lefebvre ◽  
Stéphanie Colinet ◽  
Karin Segers ◽  
René Stevens
Keyword(s):  
Cerebral Palsy ◽  
Sialic Acid ◽  
Blood Smear ◽  
Storage Disease ◽  
Smear Examination ◽  
Blood Smear Examination ◽  
Sialic Acid Storage Disease ◽  
Free Sialic Acid

scholarly journals Mini-Review Free Sialic Acid Storage Disorder: Progress and Promise

2021 ◽  
pp. 135896
Author(s):  
Marjan Huizing ◽  
Mary E. Hackbarth ◽  
David R. Adams ◽  
Melissa Wasserstein ◽  
Marc C. Patterson ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Storage Disorder ◽  
Free Sialic Acid

scholarly journals Inhibitory effect of free sialic acid on complement activation and its significance in hypocomplementemic glomerulonephritis

1999 ◽  
Vol 13 (4) ◽  
pp. 173-179 ◽  
Author(s):  
Takayuki Fujita ◽  
Atsushi Satomura ◽  
Mutsuko Hidaka ◽  
Isao Ohsawa ◽  
Morito Endo ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Complement Activation ◽  
Inhibitory Effect ◽  
Free Sialic Acid

scholarly journals EVALUATION OF THE ANTITYROSINASE AND ANTIOXIDANT POTENTIAL OF ZINC OXIDE NANOPARTICLES SYNTHESIZED FROM THE BROWN SEAWEED-TURBINARIA CONOIDES

2017 ◽  
Vol 9 (5) ◽  
pp. 116 ◽  
Author(s):  
Khoushika Raajshree R. ◽  
Brindha Durairaj
Keyword(s):  
Antioxidant Activity ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Brown Seaweed ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Skin Whitening ◽  
Algal Extract ◽  
Turbinaria Conoides ◽  
Hydroethanolic Extract

Objective: The present study was designed to evaluate the antioxidant and antityrosinase properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from brown seaweed Turbinaria conoides.Methods: Zinc Oxide Nanoparticles were synthesized from the hydroethanolic extract of Turbinaria conoides. Ultraviolet-Visible Spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology and elemental composition of ZnO-NPs were analysed using SEM-EDAX. The antioxidant activity of the synthesized zinc oxide nanoparticles was investigated by total antioxidant capacity (phosphomolybdenum method), reducing power assay and ferric reducing antioxidant power assay (FRAP). Anti tyrosinase activity was assessed to validate the skin whitening ability of the ZnO-NPs. Results: The antioxidant activity of ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides was maximum when compared with that of the hydroethanolic algal extract. The antityrosinase activity of ZnO-NPs was found to be maximum with 75% tyrosinase inhibition when compared to hydroethanolic algal extract which had 56% inhibition at 250μg/ml concentration.Conclusion: Overall our study provides a firm evidence to support that antityrosinase and antioxidant activities are exhibited by ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides and it might be used as an antioxidant and as a source of skin whitening agent in cosmetics.

Hypogammaglobulinemia and imaging features in a patient with infantile free sialic acid storage disease (ISSD) and a novel mutation in the SLC17A5 gene

2018 ◽  
Vol 31 (10) ◽  
pp. 1155-1159
Author(s):  
Tamara Žigman ◽  
Danijela Petković Ramadža ◽  
Mario Lušić ◽  
Marija Zekušić ◽  
Dorotea Ninković ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Storage Disease ◽  
Novel Mutation ◽  
Lysosomal Storage Diseases ◽  
Imaging Features ◽  
Lysosomal Storage ◽  
Biochemical Tests ◽  
Radiographic Findings ◽  
Sialic Acid Storage Disease ◽  
Free Sialic Acid

Abstract Background Infantile free sialic acid storage disease (ISSD) is a severe multisystemic disorder characterized by the accumulation of free sialic acid in lysosomes. Case presentation The patient presented prenatally with fetal ascites and large scrotal hernias, without pleural or pericardial effusion. During the infantile period, he was diagnosed with permanent isolated immunoglobulin G (IgG) hypogammaglobulinemia, which thus far has rarely been associated with ISSD. The analysis of the SLC17A5 gene revealed a novel homozygous 94 bp gene deletion. We further provide a detailed description of pre- and postnatal clinical and radiographic findings. Conclusions Fetal ascites could be the first sign of several lysosomal storage diseases (LSDs), including ISSD. The analysis of LSD gene panels is an effective approach to diagnosis in the case of non-specific symptoms and when specific biochemical tests are not easily available.

ROS-scavenging and Anti-tyrosinase Properties of Crocetin on B16F10 Murine Melanoma Cells

2018 ◽  
Vol 18 (7) ◽  
pp. 1064-1069 ◽  
Author(s):  
Seyed H. Hashemi-Shahri ◽  
Alireza Golshan ◽  
Seyed A. Mohajeri ◽  
Javad Baharara ◽  
Elaheh Amini ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Melanoma Cells ◽  
Biological Properties ◽  
B16 Melanoma ◽  
Tyrosinase Activity ◽  
Crocus Sativus ◽  
Mushroom Tyrosinase ◽  
Ros Scavenging ◽  
Protein Levels ◽  
B16 Melanoma Cells

Background: Crocus sativus (Iridaceae) has been traditionally used in the Iranian folk medicine and as a culinary additive. Major components of the plant that are responsible for biological properties are saffranal, crocin, picrocrocin and crocetin. Although the level of crocetin is not high, some of the important activities of saffron such as antioxidant activity have been attributed to crocetin. Objective: In the present study, we investigated the effects of crocetin on melanogenesis in B16 melanoma cells. Methods: The effect of crocetin on intracellular and mushroom tyrosinase activity and the content of melanin was evaluated spectrophotometrically. Tyrosinase and Microphthalmia-Associated Transcription Factor (MITF) protein levels were compared between Crocetin-treated and control cells after western blot analysis. The antioxidative activity of crocetin was also investigated. Results: Crocetin could inhibit mushroom tyrosinase activity and lower the amount of melanin in B16 melanoma cells. Protein levels of tyrosinase and MITF were also decreased by crocetin. Crocetin also showed antioxidant activity and depleted cellular Reactive Oxygen Species (ROS) content but had no cytotoxicity in alamarBlue® assay. Conclusion: Taken together, decreased tyrosinase activity, melanin content, tyrosinase and MITF proteins levels, and ROS production showed the inhibition of melanogenesis in B16F10 cells by crocetin. Hence, crocetin could be suggested as a potential dermatological whitening agent in skin care products.

Correction of the Delayed Fibrin Aggregation of Fetal Fibrinogen by Partial Removal of Sialic Acid

1979 ◽  
Author(s):  
D.K. Galanakis ◽  
M.W. Mosesson
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Acid Value ◽  
Full Term ◽  
Thrombin Time ◽  
Sialic Acid Content ◽  
Vibrio Cholera ◽  
The Mean ◽  
Fraction I ◽  
Free Sialic Acid

Human umbilical oord fibrinogen characteristically displays delayed fibrin aggregation under conditions of relatively high ionic strength. This delay is greater in fibrinogen obtained from premature (p) (24–35 weeks gestation) infants, as compared with that from full term (FT) infants. We compared the sialic acid content of (fraction I-2) fetal (P and FT) with adult (A) fibrinogen, obtained from pooled plasma. The mean sialic acid μg/100mg protein) values were: P, 818 (±135 SD, range 621–1030); FT, 720 (±212, range 505–1280); A, 626 (±110, range 487-806). One P fibrinogen preparation (thrombin time 22.6 seconds) was partially desialated (resulting sialic acid value 490) by incubation with Vibrio cholera neuraminidase, dialyzed , and precipitated with ethanol to remove free sialic acid. The thrombin time of the resulting preparation was 15.4 (range 15.2–15.8), compared to 16.1 (range 15.5–16.4) for untreated A fibrinogen. The results suggest that the delayed fibrin aggregation of fetal fibrinogen is attributable to its relatively high sialic acid content. Moreover, the intermediate sialic acid (and thrombin time) values of FT (as compared to those of p) fibrinogen intimate the presence of a mixture of adult and fetal fibrinogen in full term cord blood.

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