scholarly journals Phenolic Compound Content and the Antioxidant and Antimicrobial Activity of Wild Blueberries (Vaccinium stenophyllum Steud.) Fruits Extracts during Ripening

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 15
Author(s):  
José O. Bernal-Gallardo ◽  
Jorge Molina-Torres ◽  
María V. Angoa-Pérez ◽  
Jeanette G. Cárdenas-Valdovinos ◽  
Ignacio García-Ruíz ◽  
...  

The genus Vaccinium contains about 400 species distributed worldwide, but only a few species and varieties have had their phenolic composition and biological activity documented. In this study, phenols, flavonoids, anthocyanins, antioxidant and antimicrobial activity of methanolic extracts of Vaccinium stenophyllum Steud. fruits: 1-totally immature, 2-immature, 3-immature/close to maturity and 4-mature, were determined using UV-Visible spectrometry and HPTLC. The totally immature fruit extract showed the highest content of total phenols (19.153 ± 0.175 mg GAE/g DW), chlorogenic acid (20.867 ± 0.240 mg CAE/g DW), and the highest antioxidant activity by ABTS●+ (196.761 ± 0.641 µM TE/g DW) and DPPH● (146.580 ± 6.466 µM TE/g DW). Immature, immature/close to maturity and mature fruits extracts, exhibited the lowest MIC (9.37 mg/mL) and MBC (18.75 mg/mL) against Escherichia coli, Salmonella choleraesuis, and Shigella flexneri. The mature fruits extract exhibited the highest content of total anthocyanins (0.141 ± 0.004 mg CE/g DW) and cyanidin-3-glucoside (19.230 ± 0.309 mg CGE/g DW). The content of phenols, flavonoids and anthocyanins was higher than that reported for other Vaccinium species. These results showed the relevance of Vaccinium stenophyllum Steud. for breeding purposes to enhance the bioactivity of cultivars, or as a source of natural additives for the food industry, among others.

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Muhammad Abbas Ranjah ◽  
Amir Ismail ◽  
Muhammad Waseem ◽  
Saira Tanweer ◽  
Baila Ahmad ◽  
...  

Purpose This study aims to compare the antioxidant and antimicrobial activity of different parts (tip, mid and base portion) of lemongrass leaves for application as a natural ingredient in the functional drink. Design/methodology/approach Lemongrass leaf powder was prepared from different parts of leaves and evaluated for nutritional composition. Additionally, the extracts of different portions of lemongrass leaves were analyzed for total phenolics, free radical scavenging activities, ferric reducing antioxidant potential (FRAP) and antimicrobial activities for their application in food products. Findings Tip portion of lemongrass leaf anticipated significantly (p < 0.05) higher contents of ash, protein, calcium, potassium and iron i.e. 6.2 mg/100 g, 18 mg/100 g, 340 ppm, 819 ppm and 32 ppm, respectively. Maximum (p < 0.05) phenolics (14.7 mg GAE/100 g), 2,2-diphenyl-1-picryl-hydroxyl (86.3%) and FRAP (200 mmol/100 g) were observed in lemongrass leaf tip methanolic extracts. Moreover, lemongrass leaf tip hydro-methanolic extracts portrayed maximum zone of inhibition against E. coli and Staphylococcus aureus i.e. 16.7 and 18.2 mm, respectively. Practical implications This study demonstrated higher antimicrobial and antioxidant activities of the tip of lemongrass leaves as compared with mid and base portions, hence suggesting its role in the improvement of physicochemical, antimicrobial and antioxidant properties of food products. Consequently, the application of lemongrass methanolic extract up to 10% remarkably enhanced the nutritional value and sensorial acceptance of the beverages. Originality/value The present research draws evidence from laboratory analysis of fresh lemongrass grown in Pakistan. The findings suggest that lemongrass methanolic extracts could be used as a nutritionally rich source of antioxidant activity in functional drinks.


Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6678
Author(s):  
Artur Macari ◽  
Rodica Sturza ◽  
Ildiko Lung ◽  
Maria-Loredana Soran ◽  
Ocsana Opriş ◽  
...  

The problem of functional foods with bioactive components of natural origin is current for the food industry. Plant extracts rich in polyphenols with antioxidant and antimicrobial activity are a promising source for use in improving the quality and characteristics of fresh meat and meat products. In this context, the purpose of the present study was to evaluate the physico-chemical, microbiological, sensory properties of sausages prepared with the addition of lyophilized extract of basil, thyme or tarragon. For the beginning, the total amount of polyphenols, the antioxidant and antimicrobial activity of the extracts obtained from three spices were evaluated. In the sausages previously infected with Staphylococcus aureus and Escherichia coli it was observed that there is a much larger number of colonies of microorganisms in the control sample compared to the other samples within 24 and 48 h. Moreover, following the addition of sausage extracts, no changes were found regarding their sensory acceptability.


Although the genus Cotoneaster Medik. includes mainly ornamental species, there are some data regarding its biological activity. The purpose of this study was to analyze the content of phenolic compounds, acetylcholinesterase inhibition, antioxidant and antimicrobial activity of methanolic extracts of leaf and bark of C. integerrimus Medik., C. tomentosus (Aiton) Lindl. and C. horizontalis Decne. The C. tomentosus leaf extract exhibited the highest content of total phenols (135.86 mg GAE/g) and flavonoids (18.17 mgQE/g), and also the most potent antioxidant activity against nonbiogenic free radicals, while the highest inhibition of acetylcholinesterase had the leaf extract of C. horizontalis (IC50 0.34 mg/mL). All extracts showed a significant level of antibacterial and antifungal activity against tested microbial strains. The largest inhibition zones were observed against Candida albicans treated with C. integerrimus leaf extract (30.50±0.50 mm). Furthermore, C. integerrimus extract was the most effective in the majority of bacterial strains tested. The results indicated that methanolic extracts of the investigated Cotoneaster species have promising bioactive and therapeutic potentials


2017 ◽  
Vol 6 (2) ◽  
pp. 5245
Author(s):  
Bardees Mohammad Mickky* ◽  
Muhammad Ali Abbas ◽  
Nada Mohamed Sameh

The current investigation was conducted to evaluate the potency of salinity to enhance the nutraceutical properties of fenugreek (Trigonella foenum-graecum L.) seedlings. Gradual doses of NaCl solution (0, 50, 100, 150 and 200 mM) were applied and the nutritional value of seedlings was determined 2 and 5 days post- germination. Moreover, the antioxidant and antimicrobial activity along with the phenolic content of the considered seedlings were assessed. The obtained results manifested that salt application to different levels could increase the amount of protein, N, Zn and Cu in the 2- and 5- day old seedlings. Salinity could also raise the amount of vitamin C, B1, B2 and B9 but only in one stage. Conversely, the amount of reducing, non-reducing and total sugars as well as that of Fe declined under stress. Compared with their unsalted synonyms, water and methanolic extracts of salt- treated fenugreek seedlings had higher H2O2- scavenging activity in the two stages, while their reducing power and free radicles- scavenging activities were promoted in the second stage only. Regarding the antimicrobial activity, water and methanolic extracts of the stressed seedlings generally exhibited better antimicrobial activity against some pathogenic microbes especially Erwinia carotovora, Bacillus subtilis and Candida albicans. Only in the second stage, most of the employed salt concentrations increased the titer of tannins, saponins, alkaloids, flavonoids and total phenols in the aqueous and alcoholic extracts. Therefore, it is recommended to salinize young fenugreek seedlings to improve its health benefits in a simple, low- cost and low- risk approach.


2011 ◽  
Vol 6 (7) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
María Elena Mendiondo ◽  
Berta E. Juárez ◽  
Catiana Zampini ◽  
María Inés Isla ◽  
Roxana Ordoñez

Methanolic extracts of Chuquiraga straminea Sandwith, subfamily Barnadesioideae (Asteraceae) showed the presence of quercetin-3- O-glucoside, quercetin-3- O-rutinoside, kaempferol, kaempferol-3- O-glucoside and kaempferol-3- O-rutinoside. Antioxidant and antimicrobial activity was determined. The total extracts showed antioxidant activity by DPPH and ABTS method (SC50 14.5 to 34.9 μg/mL). A significantly positive correlation was observed between the antioxidant activity and the total phenolics (R2>0.93). The extracts were active against ten methicillin resistant and sensitive Staphylococcus aureus strains isolated from nosocomial infection (MIC values between 200 to 800 μg/mL). These preliminary studies are highly interesting as they open new ways for further applications in the treatment of infections by methicillin resistant S. aureus.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak

Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.


2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).


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