scholarly journals Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods

2021 ◽  
Vol 18 (5) ◽  
pp. 2475
Author(s):  
Francisco Ramiro Boy ◽  
Rocío Casquete ◽  
Ana Martínez ◽  
María de Guía Córdoba ◽  
Santiago Ruíz-Moyano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Native Plants ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Ultra Sound ◽  
Antihypertensive Activity ◽  
Ultrasound Extraction ◽  
Rubus Ulmifolius ◽  
Cistus Salviifolius

This study aims to evaluate the efficacy of two methods (agitation and ultra-sound) for extracting phenolic compounds from 15 native plants. Plant species collected in the Dehesa of Extremadura were used. The antioxidant, antihypertensive and antimicrobial activity of the phenolic extracts was investigated. Significantly different results were obtained when comparing the two extraction methods, with the highest concentrations of phenolic compounds found for ultrasound extraction. In addition, the extracts obtained for Cistus albidus, Cistus salviifolius, Rubus ulmifolius and Quercus ilex showed the highest concentrations of phenolic compounds. The antioxidant activity was higher in the extracts of Cistus and Q. ilex obtained by ultrasound, as was the antihypertensive activity. Antimicrobial activity was also higher in the extracts obtained by ultrasound from C. salviifolius and Q. ilex plants against bacteria and from Cistus ladanifer against yeasts. Therefore, it can be concluded that, with the ultrasound extraction of phenolic compounds from C. ladanifer, C. albidus and Q. ilex plants, it is possible to obtain extracts with important functional properties, so they could be studied for their use in food with the aim of obtaining healthy and safe products, favouring the sustainability of the environment of the Dehesa Extremeña.

scholarly journals Антимікробні властивості Tanacetum balsamita L. (Asteraceae) за інтродукції в Поліссі України

2017 ◽  
Vol 7 (1) ◽  
pp. 52-57
Author(s):  
I. V. Ivashchenko
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Minimal Bactericidal Concentration ◽  
Chromatography Method ◽  
Test Cultures

<p>The paper studies antimicrobial activity of ethanol extract of <em>Tanacetum balsamita</em> L. from the family Asteraceae, tribus Anthemideae, the plant being medicinally and pharmaceutically promising. Antimicrobial properties of <em>Tanacetum balsamita</em> were studied on test-cultures, collected from the Ukrainian Collection of Microorganisms (UCM, Institute of microbiology and virology SAS Ukraine), the test-cultures being of <em>Escherichia coli </em>(coliform bacillius) UCM B-906 (ATCC 25922); <em>Staphilococcus aureus</em> (golden staphylococcus) UCM B-904 (ATCC 25923); <em>Pseudomonas aeruginosa</em> (blue pus bacillus) UCM B-900 (ATCC 9027); <em>Candida albicans</em> UCM Y-1918 (ATCC 885-653). The above microorganisms are test strains used to determine antimicrobial properties of medicines. The antimicrobial effect of the investigated substances was studied by the method of successive dilutions which determines minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The extract has demonstrated selective antimicrobial activity against gram-positive bacteria strains of <em>Staphylococcus aureus</em>. In comparison with the solvent, MIC and MBC values showed an 8-times increase. <em>T.</em><em> </em><em>balsamita</em> extract revealed no bacteriostatic, bacteriocidal/fugicidal effect against gram-negative bacteria stains of <em>Escherichia coli, P. aeruginosa</em> and fungus <em>C. albicans</em>. The antimicrobial activity of the extract against <em>S. aureus</em> may be attributed to the presence of essential oil and phenolic compounds. The major compounds in the essential oil are epoxylynaloolacetate (60.13 %) and b-thujone (29.7 %). The following phenolic compounds have been determined with the high-performance liquid chromatography method: flavonoids – luteolin, apigenin, diosmetin, acacetin, apigenin-7-bioside, acacetin-7-bioside, apigenin-7-glycoside, luteolin-7-glycoside, diosmetin-7-bioside, diosmetin-7-glycoside, diosmetin-7-rhamnoside, and hydroxycinnamic acids – n-coumaric, caffeic, chlorogenic, isochlorogenic. The dominant compound is isochlorogenic acid (35 % of the total phenolic compounds detected). The experimental data obtained gives evidence that further research and application of <em>T. balsamita</em> is promising for creation of new anti-staphylococcus drugs.</p>

scholarly journals Emergent Technologies for the Extraction of Antioxidants from Prickly Pear Peel and Their Antimicrobial Activity

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 570
Author(s):  
Elisabete M. C. Alexandre ◽  
Marta C. Coelho ◽  
Kardelen Ozcan ◽  
Carlos A. Pinto ◽  
José A. Teixeira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolics ◽  
Ohmic Heating ◽  
Antimicrobial Properties ◽  
Growth Curves ◽  
Extraction Methods ◽  
Prickly Pear ◽  
Emergent Technologies ◽  
Viable Cells ◽  
Thermal Extraction

Phenolic compounds are important bioactive compounds identified in prickly pear peel that have important antioxidant and antimicrobial properties. However, conventional thermal extraction methods may reduce their bioactivity, and technologies such as high pressure (HP) and ohmic heating (OH) may help preserve them. In this study, both technologies were analyzed, individually and combined (250/500 MPa; 40/70 °C; ethanol concentration 30/70%), and compared with Soxhlet with regard to total phenolics, flavonoids, and carotenoids as well as antioxidant (ABTS, DPPH, ORAC), DNA pro-oxidant, and antimicrobial (inhibition halos, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curves, and viable cells) activities of prickly pear peel extracts. Total phenolics extracted by each technology increased 103% (OH) and 98% (HP) with regard to Soxhlet, but the contents of total flavonoids and carotenoids were similar. Antioxidant activity increased with HP and OH (between 35% and 63%), and OH (70 °C) did not induce DNA degradation. The phenolic compound present in higher amounts was piscidic acid, followed by eucomic acid and citrate. In general, their extraction was significantly favored by HP and OH. Antimicrobial activity against 7 types of bacteria showed effective results only against S. aureus, S. enteritidis, and B. cereus. No synergetic or additive effect was observed for HP/OH.

scholarly journals Hemp Growth Factors and Extraction Methods Effect on Antimicrobial Activity of Hemp Seed Oil: A Systematic Review

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 183
Author(s):  
Klaudia Ostapczuk ◽  
Samuel Obeng Apori ◽  
Giovani Estrada ◽  
Furong Tian
Keyword(s):  
Antimicrobial Activity ◽  
Growth Factors ◽  
Seed Oil ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Hemp Seed ◽  
Research Attention ◽  
Research Fields ◽  
Seed Flour ◽  
Dpph Scavenging

The bioactive Hemp Seed Oil (HSO) is becoming very popular in the medical and research fields due to its antimicrobial properties against several diseases caused by bacteria and fungi. However, the effect of hemp-growing factors and extraction methods on the bioactivity of HSO does not receive adequate research attention. Therefore, this review aims to investigate the effect of growth factors and extraction methods on the antimicrobial activity of HSO. Articles were retrieved from Google Scholar and the Scopus database and screened against inclusion and exclusion criteria. The study revealed that HSO prefers warm climates and favorable humidity ranging from 20 to 39 °C and 79–100% per year, respectively, and rainfall of 324 mm daily. The multivariate linear regression shown excellent prediction (R2 = 0.94) with climates upon Zone of Growth Inhibition (ZGI) of Gram-positive bacteria. Temperature is the strongest predictor (p < 0.01) followed by humidity and rainfall (p < 0.05). Furthermore, well-drained loam soil rich in organic matter seems to stimulate the antimicrobial activity of HSO. The major constituents that influence HSO’s antimicrobial ability to Staphylococcus aureus were cannabidiol (CBD), β-caryophyllene, and limonene. The extraction methods showed less influence on the HSO bioactivity. HSO did not show significant antioxidant activity, but Hemp Seed Hull (HSH), Hemp Seed Flour (HSF), and Hydrolyzed Hemp Seed Protein (HPH), expressed promising DPPH scavenging ability.

scholarly journals Optimization of Ultrasound Extraction of Cactus Pear(Opuntia ficus indica)Seed Oil Based on Antioxidant Activity and Evaluation of Its Antimicrobial Activity

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
María de los Angeles Ortega-Ortega ◽  
Nelly del Socorro Cruz-Cansino ◽  
Ernesto Alanís-García ◽  
Luis Delgado-Olivares ◽  
José Alberto Ariza-Ortega ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Extraction Method ◽  
Seed Oil ◽  
Extraction Methods ◽  
Seed Oils ◽  
Cactus Pear ◽  
Ultrasound Extraction ◽  
Conventional Extraction ◽  
Amplitude Level

The purpose of the present study was to determine the optimal ultrasound conditions (amplitude level and time) for the extraction of cactus pear seed oil with the highest antioxidant activity using a closed system. Seed oil was analyzed for yield, antioxidant activity by ABTS and DPPH, and antimicrobial activity. Conventional extraction methods were assessed for comparison. Amplitude level significantly affected antioxidant activity in linear terms (p< 0.0001 DPPH andp< 0.001 ABTS, resp.) so, at lower amplitudes, the higher antioxidant activity was achieved. The optimum ultrasound extraction conditions were of 78% amplitude for 10 min and yielded antioxidant activity values of 66.25 mg AAE/100 g and 289 µmol TE/100 g for ABTS and DPPH, respectively. Compared with conventional extraction methods, ultrasound exhibited lower oil yield and antioxidant activity but had the potential to achieve comparable results if multiple ultrasound extractions are performed in the time needed by conventional methods. Seed oils showed similar antimicrobial activity despite the extraction method and were more effective againstEscherichia coli. The results demonstrated that ultrasound can be an alternative extraction method of seed oils from fruits such as cactus pear.

scholarly journals Antimicrobial Activity of Phenolic Compounds Extracted from Platanus hybrida: Exploring Alternative Therapies for a Post-Antibiotic Era

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 18
Author(s):  
Jessica Ribeiro ◽  
Vanessa Silva ◽  
Alfredo Aires ◽  
Rosa Carvalho ◽  
Gilberto Igrejas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Salmonella Enteritidis ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Tree Trunk ◽  
Bacterial Strains ◽  
Antibiotic Resistant

Multidrug-resistant bacteria are a significant threat to public health and new classes of antibiotics and approaches to treatment are needed. Several studies have shown that natural plant-derived compounds could be a promising mean to fight microbial resistance but only a few were conducted with antibiotic resistant bacteria. Therefore, the aim of this study was to extract phenolic compounds from the leaves, fruits, and tree trunk of Platanus hybrida and evaluate their antimicrobial activity against antibiotic resistant bacterial strains. The polyphenolic compounds were extracted using a water/ethanol (20:80) mixture. Two grams of powder of each sample was extracted with 100 mL of solvent by stirring for 2h. The extracts were redissolved in dimethyl sulfoxide (DMSO) to a final concentration of 100 mg/mL. An antimicrobial susceptibility assay was performed using the Kirby–Bauer disc diffusion method and was tested against ten different bacteria: Listeria monocytes, Bacillus cereus, Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. The fruits had the highest antibacterial activity showing a minimum inhibitory concentration (MIC) of 10mg/mL, contrary to the tree trunk that showed the lowest antibacterial activity. None of the extracts showed antimicrobial properties against S. enteritidis, E. faecium and E. faecalis. These results show that P. hybrida’s phenolic compounds act as antibacterial agents, which may become useful therapeutic tools and represent a source for the development of novel antimicrobials. However, they were not effective against all bacteria, which shows that polyphenols alone might not substitute antibiotics.

scholarly journals Antimicrobial activity of berries extracts of four Ribes species, their phenolic content and anthocyanin composition

2018 ◽  
Vol 30 (2) ◽  
pp. 249-257 ◽  
Author(s):  
Vidmantas Bendokas ◽  
Antanas Šarkinas ◽  
Daiva Jasinauskienë ◽  
Nijolë Anisimovienë ◽  
Šarûnë Morkûnaitë-Haimi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Phenolic Content ◽  
Antimicrobial Properties ◽  
Anthocyanin Content ◽  
Gram Negative Bacteria ◽  
Processing Plants ◽  
Berry Extracts ◽  
Anthocyanin Composition

Abstract Phenolic compounds are widespread in berries and determine their antimicrobial activity. The aim of our study was to establish the amounts of phenolic compounds and the anthocyanin composition in berries of four Ribes species, and to evaluate the effect of berry extracts on the growth of common Gram-positive and Gram-negative bacteria, and also yeasts isolated from food processing plants. The phenolic content and anthocyanin composition were estimated spectrometrically and by HPLC, respectively. The highest amount of phenolic compounds, and also anthocyanins, was found in extracts of R. aureum ‘Corona’. The anthocyanin content was the lowest in berries of R. aureum Au Gs-5, with equal amounts of delphinidins and cyanidins. Delphinidins were predominant (68.6%) in berries of R. nigrum ‘Ben Tirran’, while cyanidins dominated in R. uva-crispa. The berry extracts of R. aureum Au Gs-5 and R. uva-crispa ‘Lûðiai’ had the largest growth-suppressing effect on yeasts and most of the bacteria tested. All of the berry extracts suppressed the growth of pathogenic and conditionally pathogenic bacteria. The industrially important Lactococcus lactis was the most resistant to the Ribes berry extracts. There was no correlation between the amount of anthocyanins in the extracts and their antimicrobial properties. Extracts with a lower anthocyanin–to-phenolics ratio more effectively inhibited the growth of bacteria.

scholarly journals The influence of extraction method on the composition and analgesic activity of Calligonum comosum phenolic extracts

2020 ◽  
Vol 31 (1) ◽  
pp. 33-37
Author(s):  
Atef Chouikh ◽  
Abdelkrim Rebiai
Keyword(s):  
Phenolic Compounds ◽  
Analgesic Activity ◽  
Hplc Analysis ◽  
Extraction Methods ◽  
Ultra Sound ◽  
Total Phenolic ◽  
Control Group ◽  
High Concentration ◽  
Ethanol Extracts ◽  
Phenolic Extracts

AbstractThe aim of this study was to evaluate the analgesic activity and the effect of extraction methods (ultra-sound: UM and maceration: MM) and solvents (ethanol: EtOH and methanol: MeOH) on the composition of phenolic extracts from Calligonum comosum. The results obtained by HPLC analysis demonstrated that the ethanol extracts have shown the highest content of total phenolic and flavonoid compounds. Also, the presence of most known phenolic compounds has been identified in all extracts, especially in the MeOH UM extract. The HPLC analysis showed the presence of ascorbic acid in methanol extracts and caffeic acid in ethanol extracts, and the maceration method shows a high concentration of phenolic compounds, the vanillin was detected in MeOH UM and the appearance of chlorogenic acid in UM extracts, finally the emergence of gallic acid, quercetin and rutin in some extracts. According to the results of the analgesic power, the methanolic extract of the maceration method induces a significant decrease in abdominal cramps compared to the control group and the values obtained are very close from those obtained with the standard anti-inflammatory drug (indomethacin). This result confirmed the beneficial effect of this Saharan plant.

scholarly journals Preliminary evaluation of the antimicrobial activity of different Hermetia illucens larvae extracts for application as a cosmetic ingredient

2020 ◽  
Vol 17 (2) ◽  
pp. 1-10
Author(s):  
Cíntia Almeida ◽  
Daniel Murta ◽  
Rui Nunes ◽  
André Rolim Baby ◽  
Patrícia Rijo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lipid Fraction ◽  
Current Trend ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Preliminary Evaluation ◽  
Hermetia Illucens ◽  
Crude Extracts ◽  
High Lipid ◽  
High Concentrations

The cosmetic industry is continually searching for innovative ingredients, and there is a current trend in sourcing materials of natural origin. The Hermetia illucens (Linnaeus) larvae biomass is a potential source of compounds with applicability in cosmetics due to its high lipid, protein, and polysaccharide content. High concentrations of lauric acid, also known for also having antimicrobial properties, may be obtained from the lipid fraction. Additionally, these larvae are known to have a well-developed immune system, surviving in decomposition habitats, and can be induced to produce antimicrobial peptides (AMP's). These substances could be probed as alternative preservatives in cosmetics, as consumers are often concerned with the safety of conventional preservatives, such as parabens. The present study conducted a preliminary assessment of the antimicrobial activity of the crude extracts obtained from different extraction methods and extraction solvents against four microorganisms (Gram-positive and negative bacteria and yeasts). The use of diluted extracts did not show relevant inhibition of the growth of the tested microorganisms compared to the positive controls. Further assays are foreseen to test the antimicrobial activity of compounds or fractions isolated from the crude extracts.

scholarly journals Value-Added Compound Recovery from Invasive Forest for Biofunctional Applications: Eucalyptus Species as a Case Study

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4227
Author(s):  
Patricia Gullón ◽  
Beatriz Gullón ◽  
Gonzalo Astray ◽  
Paulo E. S. Munekata ◽  
Mirian Pateiro ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Biological Activities ◽  
Folk Medicine ◽  
Antimicrobial Properties ◽  
Value Added ◽  
Extraction Methods ◽  
Eucalyptus Species ◽  
Upper Respiratory Tract ◽  
Avant Garde ◽  
Increasing Demand

From ancient times, the medicinal properties of the different Eucalyptus species are well known. In fact, plants from this family have been used in folk medicine as antiseptics, and to treat different ailments of the upper respiratory tract such as sinus congestion, common cold, or influenza. Moreover, other biological activities were described for Eucalyptus species such as antioxidant and antimicrobial properties. In the last few decades, numerous investigations revealed that the compounds responsible for these properties are secondary metabolites that belonging to the group of phenolic compounds and are present in different parts of the plants such as leaves, bark, wood, fruits, and stumps. The increasing demand for natural compounds that can substitute synthetic antioxidants and the increase in resistance to traditional antibiotics have boosted the intense search for renewable natural sources containing substances with such bioactivities, as well as greener extraction technologies and avant-garde analytical methods for the identification of the target molecules. The literature data used in this paper were collected via Scopus (2001–2020) using the following search terms: Eucalyptus, extraction methods, phenolic compounds, and biological activities. This review collects the main studies related to the recovery of value-added compounds from different Eucalyptus species, as well as their biofunctional applications.

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