In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells

Plant-associated microorganisms represent a potential source of new antitumor compounds. The aim of the present study was to isolate endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae and produce extracts with antitumor activity. Methanol and ethyl acetate extracts were obtained from 28 d bacterial fermentation, after which murine L5178Y-R lymphoma cells growth inhibition was evaluated at concentrations ranging from 15.62 µg/mL to 500 µg/mL by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide reduction colorimetric assay. IC50 and the selectivity index (SI) were calculated and compared with healthy control human peripheral blood mononuclear cells (PBMC). Identification of the isolated strains was performed using the 16S ribosomal gene and by MALDI-TOF MS mass spectrometry. The endophytic and rhizosphere bacterial extracts from strains ISE-B22, ISE-B26, ISE-B27, ISS-A01, ISS-A06, and ISS-A16 showed significant (p < 0.05) L5178Y-R cell growth inhibition, compared with an untreated control. The rhizosphere Micromonospora echinospora isolate ISS-A16 showed the highest (90.48%) percentage of lymphoma cells growth inhibition and SI (19.1) for PBMC, whereas the Bacillus subtilis ISE-B26 isolate caused significant (p < 0.01) growth inhibition (84.32%) and a SI of 5.2. Taken together, results of the present study evidenced antitumor effects by I. sonorae endophytic and rhizosphere bacteria culture extracts. Further research will involve the elucidation of the compounds that exert the antitumor activity and their evaluation in pre-clinical studies.

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The L-Selectin Antibody FMC46 Mediates Rapid, Transient Increase in Intracellular Calcium in Human Peripheral Blood Mononuclear Cells and Daudi Lymphoma Cells

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1995.2888 ◽

1995 ◽

Vol 217 (3) ◽

pp. 1145-1150 ◽

Cited By ~ 9

Author(s):

J.L. Po ◽

B. Mazer ◽

G.S. Jensen

Keyword(s):

Intracellular Calcium ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Transient Increase ◽

Lymphoma Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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The antitumor effects of locally injecting human peripheral blood mononuclear cells treated with OK-432 into the tumor site: The possible role of a tumor growth inhibitory factor (TGIF)

The Japanese Journal of Surgery ◽

10.1007/bf02470717 ◽

1990 ◽

Vol 20 (1) ◽

pp. 76-82 ◽

Cited By ~ 4

Author(s):

Mitsuo Katano ◽

Tetsuro Mizoguchi ◽

Hiroshi Yamamoto ◽

Mitsunari Nakamura ◽

Tatsuya Matsuo ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Tumor Site ◽

Antitumor Effects ◽

Peripheral Blood Mononuclear ◽

Growth Inhibitory Factor ◽

Growth Inhibitory ◽

Blood Mononuclear Cells

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Studies on OK-432-induced antitumor activity in human peripheral blood mononuclear cells.

Japanese Journal of Oral & Maxillofacial Surgery ◽

10.5794/jjoms.38.1815 ◽

1992 ◽

Vol 38 (12) ◽

pp. 1815-1822 ◽

Cited By ~ 1

Author(s):

Eiro KUBOTA ◽

Hideo IMAMURA ◽

Mitsuo KATANO ◽

Hiroyuki KUROKAWA ◽

Kinuko TANAKA ◽

...

Keyword(s):

Antitumor Activity ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Optimisation, harmonisation and standardisation of the direct mycobacterial growth inhibition assay using cryopreserved human peripheral blood mononuclear cells

Journal of Immunological Methods ◽

10.1016/j.jim.2019.01.006 ◽

2019 ◽

Vol 469 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Rachel Tanner ◽

Steven G. Smith ◽

Krista E. van Meijgaarden ◽

Federico Giannoni ◽

Morven Wilkie ◽

...

Keyword(s):

Growth Inhibition ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Human Peripheral Blood ◽

Inhibition Assay ◽

Peripheral Blood Mononuclear ◽

Growth Inhibition Assay ◽

Blood Mononuclear Cells ◽

Mycobacterial Growth

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Differential Cytokine Response in Host Defence Mechanisms Triggered by Gram-Negative and Gram-Positive Bacteria, and the Roles of Gabexate Mesilate, a Synthetic Protease Inhibitor

Journal of International Medical Research ◽

10.1177/147323000203000201 ◽

2002 ◽

Vol 30 (2) ◽

pp. 99-108 ◽

Cited By ~ 13

Author(s):

H Iwadou ◽

Y Morimoto ◽

H Iwagaki ◽

S Sinoura ◽

Y Chouda ◽

...

Keyword(s):

Protease Inhibitor ◽

Bacterial Infections ◽

Mononuclear Cells ◽

Cytokine Production ◽

Gabexate Mesilate ◽

Gram Positive ◽

Peripheral Blood Mononuclear ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Tnf Α

Bacterial infection results in the production of inflammatory mediators and may be involved in the pathogenesis of sepsis and/or systemic inflammatory response syndrome. The effect of lipopolysaccharide (LPS), a major component of the outer surface of Gram-negative bacteria, and Staphylococcal enterotoxin B (SEB), a superantigen of Gram-positive bacteria, on cytokine production in peripheral blood mononuclear cells (PBMCs) was examined. LPS significantly increased the production of proinflammatory and anti-inflammatory cytokines, and SEB enhanced the production of helper T lymphocyte type cytokines. These results illustrated the different responses to Gram-negative and Gram-positive bacterial infections. The effect of gabexate mesilate, a synthetic protease inhibitor, on cytokine production and expression of the toll-like receptor (TLR) was also examined. The results suggest that gabexate mesilate-induced inhibition of tumour necrosis factor-α (TNF-α) and interleukin-18 (IL-18) production in LPS-stimulated PBMCs is due to the inhibition of the nuclear factor-κB activation pathway and/or inhibition of the processing pathway of pro-TNF-α and pro-IL-18, not to down-regulation of TLR-2 or TLR-4.

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