scholarly journals Optimization of the Culture Medium Composition to Improve the Production of Hyoscyamine in Elicited Datura stramonium L. Hairy Roots Using the Response Surface Methodology (RSM)

2010 ◽  
Vol 11 (11) ◽  
pp. 4726-4740 ◽  
Author(s):  
Ryad Amdoun ◽  
Lakhdar Khelifi ◽  
Majda Khelifi-Slaoui ◽  
Samia Amroune ◽  
Mark Asch ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Hairy Roots ◽  
Culture Medium ◽  
Datura Stramonium ◽  
Medium Composition

Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by Kocuria rosea

2006 ◽  
Vol 52 (5) ◽  
pp. 445-450 ◽  
Author(s):  
C Bernal ◽  
I Diaz ◽  
N Coello
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Fermentation ◽  
Culture Medium ◽  
Medium Optimization ◽  
Fold Increase ◽  
Medium Composition ◽  
Central Composite Experimental Design ◽  
Kocuria Rosea ◽  
Central Composite

A 43-fold increase in keratinase production by Kocuria rosea was achieved in batch fermentation using response surface methodology. Factorial designs were used to select the components of a culture medium that showed a significant effect on keratinase production. An orthogonal–central composite experimental design was performed, with only two (feathers and magnesium) from nine initial compounds being further analyzed by response surface methodology. An optimum keratinase production of 14 886.9 U/mg was obtained with the following medium composition (per litre): NH4Cl, 0.3 g; NaCl, 0.3 g; K2HPO4, 3.2 g; KH2PO4, 4.0 g; MgSO4·6H2O, 0.5 g; yeast extract, 0.1 g; and finely milled feathers, 30 g. The medium was shaken at 400 r/min with an incubation period of 14 h at 40 °C.Key words: feathers, keratinases, Kocuria rosea, medium optimization, response surface methodology.

scholarly journals Optimization of medium composition for probiotic powder inoculum using the response surface methodology

2021 ◽  
Vol 788 (1) ◽  
pp. 012038
Author(s):  
Rohmatussolihat ◽  
R Ridwan ◽  
Y Widyastuti ◽  
N F Sari ◽  
R Fidryanto ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Medium Composition
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