scholarly journals Adult Stem Cells Spheroids to Optimize Cell Colonization in Scaffolds for Cartilage and Bone Tissue Engineering

2018 ◽  
Vol 19 (5) ◽  
pp. 1285 ◽  
Author(s):  
Leandra Baptista ◽  
Gabriela Kronemberger ◽  
Isis Côrtes ◽  
Letícia Charelli ◽  
Renata Matsui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adult Stem Cells ◽  
Cell Colonization

Wrapped omentum with periosteum concurrent with adipose derived adult stem cells for bone tissue engineering in dog model

2013 ◽  
Vol 15 (1) ◽  
pp. 127-137 ◽  
Author(s):  
Amin Bigham Sadegh ◽  
Ehsan Basiri ◽  
Ahmad Oryan ◽  
Pezhman Mirshokraei
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adult Stem Cells ◽  
Dog Model

Synthesis of calcium phosphate-zirconia scaffold and human endometrial adult stem cells for bone tissue engineering

2014 ◽  
Vol 44 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Aliakbar Alizadeh ◽  
Fathollah Moztarzadeh ◽  
Seyed Naser Ostad ◽  
Mahmoud Azami ◽  
Bita Geramizadeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adult Stem Cells

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

2018 ◽  
Vol 233 (10) ◽  
pp. 6509-6522 ◽  
Author(s):  
Sadegh L. Moradi ◽  
Ali Golchin ◽  
Zahra Hajishafieeha ◽  
Mohammad-Mehdi Khani ◽  
Abdolreza Ardeshirylajimi
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Adult Stem Cells ◽  
Nanofibrous Scaffolds

Osteogenic potential of dental and oral derived stem cells in bone tissue engineering among animal models: An update

2021 ◽  
Vol 71 ◽  
pp. 101515
Author(s):  
Antoine Berbéri ◽  
Mohammad Fayyad-kazan ◽  
Sara Ayoub ◽  
Rita Bou Assaf ◽  
Joseph Sabbagh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Animal Models ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Osteogenic Potential

scholarly journals A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Thakoon Thitiset ◽  
Siriporn Damrongsakkul ◽  
Supansa Yodmuang ◽  
Wilairat Leeanansaksiri ◽  
Jirun Apinun ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Alp Activity

Abstract Background A novel biodegradable scaffold including gelatin (G), chitooligosaccharide (COS), and demineralized bone matrix (DBM) could play a significant part in bone tissue engineering. The present study aimed to investigate the biological characteristics of composite scaffolds in combination of G, COS, and DBM for in vitro cell culture and in vivo animal bioassays. Methods Three-dimensional scaffolds from the mixture of G, COS, and DBM were fabricated into 3 groups, namely, G, GC, and GCD using a lyophilization technique. The scaffolds were cultured with mesenchymal stem cells (MSCs) for 4 weeks to determine biological responses such as cell attachment and cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, cell morphology, and cell surface elemental composition. For the in vivo bioassay, G, GC, and GCD, acellular scaffolds were implanted subcutaneously in 8-week-old male Wistar rats for 4 weeks and 8 weeks. The explants were assessed for new bone formation using hematoxylin and eosin (H&E) staining and von Kossa staining. Results The MSCs could attach and proliferate on all three groups of scaffolds. Interestingly, the ALP activity of MSCs reached the greatest value on day 7 after cultured on the scaffolds, whereas the calcium assay displayed the highest level of calcium in MSCs on day 28. Furthermore, weight percentages of calcium and phosphorus on the surface of MSCs after cultivation on the GCD scaffolds increased when compared to those on other scaffolds. The scanning electron microscopy images showed that MSCs attached and proliferated on the scaffold surface thoroughly over the cultivation time. Mineral crystal aggregation was evident in GC and greatly in GCD scaffolds. H&E staining illustrated that G, GC, and GCD scaffolds displayed osteoid after 4 weeks of implantation and von Kossa staining confirmed the mineralization at 8 weeks in G, GC, and GCD scaffolds. Conclusion The MSCs cultured in GCD scaffolds revealed greater osteogenic differentiation than those cultured in G and GC scaffolds. Additionally, the G, GC, and GCD scaffolds could promote in vivo ectopic bone formation in rat model. The GCD scaffolds exhibited maximum osteoinductive capability compared with others and may be potentially used for bone regeneration.

Dextran-coated fluorapatite nanorods doped with lanthanides in labelling and directing osteogenic differentiation of bone marrow mesenchymal stem cells

2014 ◽  
Vol 2 (23) ◽  
pp. 3609-3617 ◽  
Author(s):  
Haifeng Zeng ◽  
Xiyu Li ◽  
Fang Xie ◽  
Li Teng ◽  
Haifeng Chen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Novel Approach ◽  
Marrow Mesenchymal Stem Cells

A novel approach for labelling and tracking BMSCs in bone tissue engineering by using dextran-coated fluorapatite nanorods doped with lanthanides.

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