scholarly journals Key Regulators of Sucrose Metabolism Identified Through Comprehensive Comparative Transcriptome Analysis in Peanuts

2021 ◽  
Vol 22 (14) ◽  
pp. 7266
Author(s):  
Weitao Li ◽  
Li Huang ◽  
Nian Liu ◽  
Manish K. Pandey ◽  
Yuning Chen ◽  
...  
Keyword(s):  
Seed Development ◽  
Transcriptome Analysis ◽  
Molecular Basis ◽  
Recombinant Inbred Lines ◽  
Sucrose Metabolism ◽  
Sucrose Content ◽  
Comparative Transcriptome ◽  
Peanut Seed ◽  
Fructose 1,6 Bisphosphate ◽  
High Sucrose

Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts.

scholarly journals Development and application of a near infrared spectroscopy model for predicting high sucrose content of peanut seed

2020 ◽  
Vol 47 (2) ◽  
pp. 332-341
Author(s):  
Yong LEI ◽  
Zhi-Hui WANG ◽  
Dong-Xin HUAI ◽  
Hua-Yuan GAO ◽  
Li-Ying YAN ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Sucrose Content ◽  
Peanut Seed ◽  
High Sucrose

scholarly journals Transcriptome analysis identifies differentially expressed genes involved in the metabolic regulatory network of progenies from the cross of low phytic acid GmMIPS1 and GmIPK1 soybean mutants

2020 ◽  
Author(s):  
Hangxia Jin ◽  
Xiaomin Yu ◽  
Qinghua Yang ◽  
Fengjie Yuan ◽  
Xujun Fu
Keyword(s):  
Phytic Acid ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Defense Mechanisms ◽  
Developmental Stages ◽  
Sucrose Metabolism ◽  
Differentially Expressed ◽  
Low Phytic Acid ◽  
Advanced Generation ◽  
High Sucrose

Abstract Lowering the phytic acid (PA) content of crop seeds will be beneficial for improving their nutritional traits. Low phytic acid (lpa) crop lines carrying more than one independent mutated gene have been shown to exhibit more pronounced reductions of PA content than mutants with a single lpa mutated gene. But little is known about the link between PA pathway intermediates and downstream regulation following mutation of these genes in soybean. Here, we performed a comparative transcriptome analysis using an advanced-generation recombinant inbred line [2mlpa (mips1/ipk1)] with low PA and a sibling line with homozygous non-mutant alleles and normal PA [2MWT (MIPS1/IPK1)]. RNA sequencing revealed differential expression levels of numerous genes between seeds of 2mlpa and 2MWT at five developmental stages. A total of 7,945 differentially expressed genes were identified. 3316 DEGs were in 128 metabolic and signal transduction pathways and 4980 DEGs were classified into 345 function terms associated with biological processes. Genes associated with PA metabolism, photosynthesis, starch and sucrose metabolism, and defense mechanisms were related to low PA in 2mlpa soybean line. Among these, 36 genes were up/down-regulated in PA metabolic processes, with 22 possibly contributing to the low PA phenotype of 2mlpa. Most of the genes (81 of 117) associated with photosynthesis were down-regulated in 2mlpa at the late seed stage. Three genes involved in sucrose metabolism were up-regulated at the late seed stage, which might explain the high sucrose content of 2mlpa soybeans. Additionally, 604 genes related to defense mechanisms were differentially expressed between 2mlpa and 2MWT. In this research, the soybean mutant 2mlpa was found to not only exhibit low PA but also have changes in multiple metabolites and secondary metabolites. The results delineate the regulation of these metabolic events by 2mlpa. Many genes associated with PA metabolism would contribute to the drastic reduction of PA and moderate accumulation of InsP3-InsP5 in 2mlpa mutant. And other regulated genes found in photosynthesis, starch and sucrose metabolism, and defense mechanisms would give us more insight into the nutritional and agronomic performance of 2mlpa.

Transcriptome Analysis of Low- and High-Sucrose Pear Cultivars Identifies Key Regulators of Sucrose Biosynthesis in Fruits

2020 ◽  
Vol 61 (8) ◽  
pp. 1493-1506
Author(s):  
Jiahong Lü ◽  
Xin Tao ◽  
Gaifang Yao ◽  
Shaoling Zhang ◽  
Huping Zhang
Keyword(s):  
Transcriptome Analysis ◽  
Fruit Development ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Linear Regression Analysis ◽  
Sucrose Phosphate Synthase ◽  
Sucrose Accumulation ◽  
Sucrose Content ◽  
Sucrose Biosynthesis ◽  
High Sucrose

Abstract Sucrose accumulation is one of the important factors that determine fruit enlargement and quality. Evaluation of the sugar profile of 105 pear cultivars revealed low-sucrose and high-sucrose (HS) types of pear fruits. To better understand the molecular mechanisms governing the sucrose content of pear fruits, this study performed transcriptome analysis during fruit development using low-sucrose ‘Korla’ fragrant pear and HS ‘Hosui’ pear, and a coexpression module uniquely associated with the control of high-sucrose accumulation was identified by weighted gene coexpression network analysis. These results suggested that there are seven candidate genes encoding key enzymes (fructokinase, glucose-6-phosphate isomerase, sucrose phosphate synthase and sucrose synthase) involved in sucrose biosynthesis and several transcription factors (TFs) whose expression patterns correlate with those of genes associated with sucrose biosynthesis. This correlation was confirmed by linear regression analysis between predicted gene expression and sucrose content in different pear cultivars during fruit development. This study provides insight into the molecular mechanism underlying differences in sucrose content across pear cultivars and presents candidate structural genes and TFs that could play important roles in regulating carbohydrate partitioning and sucrose accumulation.

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