scholarly journals Comparative Metabolomic Study of Drosophila Species with Different Lifespans

2021 ◽  
Vol 22 (23) ◽  
pp. 12873
Author(s):  
Dmitry L. Maslov ◽  
Nadezhda V. Zemskaya ◽  
Oxana P. Trifonova ◽  
Steven Lichtenberg ◽  
Elena E. Balashova ◽  
...  

The increase in life expectancy, leading to a rise in the proportion of older people, is accompanied by a prevalence of age-related disorders among the world population, the fight against which today is one of the leading biomedical challenges. Exploring the biological insights concerning the lifespan is one of the ways to provide a background for designing an effective treatment for the increase in healthy years of life. Untargeted direct injection mass spectrometry-based metabolite profiling of 12 species of Drosophila with significant variations in natural lifespans was conducted in this research. A cross-comparison study of metabolomic profiles revealed lifespan signatures of flies. These signatures indicate that lifespan extension is associated with the upregulation of amino acids, phospholipids, and carbohydrate metabolism. Such information provides a metabolome-level view on longevity and may provide a molecular measure of organism age in age-related studies.

2021 ◽  
pp. 971-978
Author(s):  
B. Farneti ◽  
I. Khomenko ◽  
M. Pietrella ◽  
P. Martinatti ◽  
F. Emanuelli ◽  
...  

2018 ◽  
Vol 25 (2) ◽  
pp. 251-258 ◽  
Author(s):  
Estelle Rathahao-Paris ◽  
Sandra Alves ◽  
Nawel Boussaid ◽  
Nicole Picard-Hagen ◽  
Véronique Gayrard ◽  
...  

Direct injection–mass spectrometry can be used to perform high-throughput metabolomic fingerprinting. This work aims to evaluate a global analytical workflow in terms of sample preparation (urine sample dilution), high-resolution detection (quality of generated data based on criteria such as mass measurement accuracy and detection sensitivity) and data analysis using dedicated bioinformatics tools. Investigation was performed on a large number of biological samples collected from sheep infected or not with scrapie. Direct injection–mass spectrometry approach is usually affected by matrix effects, eventually hampering detection of some relevant biomarkers. Reference compounds were spiked in biological samples to help evaluate the quality of direct injection–mass spectrometry data produced by Fourier Transform mass spectrometry. Despite the potential of high-resolution detection, some drawbacks still remain. The most critical is the presence of matrix effects, which could be minimized by optimizing the sample dilution factor. The data quality in terms of mass measurement accuracy and reproducible intensity was evaluated. Good repeatability was obtained for the chosen dilution factor (i.e., 2000). More than 150 analyses were performed in less than 16 hours using the optimized direct injection–mass spectrometry approach. Discrimination of different status of sheeps in relation to scrapie infection (i.e., scrapie-affected, preclinical scrapie or healthy) was obtained from the application of Shrinkage Discriminant Analysis to the direct injection–mass spectrometry data. The most relevant variables related to this discrimination were selected and annotated. This study demonstrated that the choice of appropriated dilution faction is indispensable for producing quality and informative direct injection–mass spectrometry data. Successful application of direct injection–mass spectrometry approach for high throughput analysis of a large number of biological samples constitutes the proof of the concept.


2021 ◽  
pp. 338880
Author(s):  
Yan Sun ◽  
Stephanie Stransky ◽  
Jennifer Aguilan ◽  
Sanjay Koul ◽  
Scott J. Garforth ◽  
...  

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 815-815
Author(s):  
Wenjun Li ◽  
Vivian Lou

Abstract Asians are the largest and the fastest growing segment of the world population, and the second largest immigrant population in the U.S. A high proportion of Asians live in developing countries or are immigrants to developed countries. Studies on age-related social and health issues of Asians have lagged. To advance research into aging among Asians living in the U.S. and elsewhere in the world, sound methodologies can be adopted from those well-developed in other settings while novel methodologies are to be developed to meet the unique needs of Asian studies. This symposium brings together five abstracts that address a variety of innovative methodological issues in social and health studies among Asian older adults. The topics range from use of wearable devices to measure travel patterns, life space and physical activity in late-life Asian immigrants in the U.S.; use of Ecological Momentary Assessment method to examine influences of social contact satisfaction on neighborhood affect in Chinese in mainland China; use of multilingual age-friendly touch-screen device for cognitive intervention in Singapore; use of GIS to evaluate potential influences of accessibility to neighborhood living resources on attitudes towards aging among the oldest old in Shanghai; and use of a multi-phase complex mixed methods to evaluate a Chinese cancer screening program in Taiwan. Together, these studies showed that innovative methods can be adapted for context relevance and linguistic and cultural appropriateness, and successfully executed in studies on aging among Asians. This symposium is a collaborative effort of the Aging Among Asians Interest Group.


Talanta ◽  
2007 ◽  
Vol 72 (1) ◽  
pp. 256-262 ◽  
Author(s):  
J PAVON ◽  
M SANCHEZ ◽  
M LAESPADA ◽  
C PINTO ◽  
B CORDERO

2016 ◽  
Vol 147 (8) ◽  
pp. 1385-1391 ◽  
Author(s):  
Václav Matěj Bierhanzl ◽  
Radomír Čabala ◽  
Martin Ston ◽  
Peter Kotora ◽  
Viktória Ferenczy ◽  
...  

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