Mycobacterium chelonae Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report

We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with Mycobacterium chelonae, which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples.

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A cloud-compatible bioinformatics pipeline for ultrarapid pathogen identification from next-generation sequencing of clinical samples

Genome Research ◽

10.1101/gr.171934.113 ◽

2014 ◽

Vol 24 (7) ◽

pp. 1180-1192 ◽

Cited By ~ 285

Author(s):

S. N. Naccache ◽

S. Federman ◽

N. Veeraraghavan ◽

M. Zaharia ◽

D. Lee ◽

...

Keyword(s):

Next Generation Sequencing ◽

Clinical Samples ◽

Next Generation ◽

Pathogen Identification ◽

Bioinformatics Pipeline ◽

Generation Sequencing

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Anomalous origin of right pulmonary artery from ascending aorta - A case report

MedPulse International Journal of Radiology ◽

10.26611/10131031 ◽

2019 ◽

Vol 10 (3) ◽

pp. 13-14

Author(s):

Manohar B Kachare ◽

◽

Vijay C Nalpe ◽

Ravi Raval ◽

◽

...

Keyword(s):

Case Report ◽

Pulmonary Artery ◽

Ascending Aorta ◽

Anomalous Origin ◽

Right Pulmonary Artery

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Case Report: Next generation sequencing identifies a NAB2-STAT6 fusion in Glioblastoma

Diagnostic Pathology ◽

10.1186/s13000-016-0455-9 ◽

2016 ◽

Vol 11 (1) ◽

Cited By ~ 6

Author(s):

Phedias Diamandis ◽

Ruben Ferrer-Luna ◽

Raymond Y. Huang ◽

Rebecca D. Folkerth ◽

Azra H. Ligon ◽

...

Keyword(s):

Case Report ◽

Next Generation Sequencing ◽

Next Generation ◽

Generation Sequencing

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Primary splenic histiocytic sarcoma associated with hemophagocytic lymphohistiocytosis: A case report and review of literature of next‐generation sequencing involving FLT3 , NOTCH2 , and KMT2A mutations

Cancer Reports ◽

10.1002/cnr2.1496 ◽

2021 ◽

Author(s):

Nelson Montalvo ◽

Jorge Lara‐Endara ◽

Ligia Redrobán ◽

María Leiva ◽

Christian Armijos ◽

...

Keyword(s):

Case Report ◽

Next Generation Sequencing ◽

Hemophagocytic Lymphohistiocytosis ◽

Histiocytic Sarcoma ◽

Review Of Literature ◽

Next Generation ◽

Generation Sequencing

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Catching a floating thrombus; a case report on the treatment of a large thrombus in the ascending aorta

Journal of Cardiothoracic Surgery ◽

10.1186/s13019-017-0600-x ◽

2017 ◽

Vol 12 (1) ◽

Cited By ~ 8

Author(s):

Gijs Eduard de Maat ◽

Giorgio Vigano ◽

Massimo Alessandro Mariani ◽

Ehsan Natour

Keyword(s):

Case Report ◽

Ascending Aorta ◽

Floating Thrombus

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A case report demonstrating the utility of next generation sequencing in analyzing serial samples from the lung following an infection with influenza A (H7N9) virus

Journal of Clinical Virology ◽

10.1016/j.jcv.2015.12.013 ◽

2016 ◽

Vol 76 ◽

pp. 45-50 ◽

Cited By ~ 3

Author(s):

Yongfeng Hu ◽

Yan Zhang ◽

Xianwen Ren ◽

Yingmei Liu ◽

Yan Xiao ◽

...

Keyword(s):

Case Report ◽

Next Generation Sequencing ◽

Influenza A ◽

H7n9 Virus ◽

Next Generation ◽

Serial Samples ◽

Generation Sequencing

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An Unusual Femoral Sheath Severing with Successful Recapture: A Case Report

Reports ◽

10.3390/reports4040034 ◽

2021 ◽

Vol 4 (4) ◽

pp. 34

Author(s):

Frane Runjić ◽

Andrija Matetic ◽

Matjaž Bunc ◽

Nikola Crnčević ◽

Ivica Kristić

Keyword(s):

Case Report ◽

Aortic Valve ◽

Aortic Valve Replacement ◽

Transcatheter Aortic Valve Replacement ◽

Balloon Catheter ◽

Proximal Part ◽

Ascending Aorta ◽

Introducer Sheath ◽

Transcatheter Aortic Valve ◽

Coronary Cusp

This study presents a case of a successful severed femoral sheath recapture during transfemoral transcatheter aortic valve replacement (TAVR). During skin tunneling with a scalpel, the discontinuity of the femoral sheath occurred. Grasping of the distal sheath with the surgical hemostat was attempted unsuccessfully. A proximal part of the severed sheath was removed and Medtronic Sentrant introducer sheath (14 French) was then placed over the existing Confida wire which permanently remained in position, followed by the introduction of the Amplatz Left 2 (AL2) catheter which pushed the severed sheath in the ascending aorta over the Confida wire. The crucial maneuver was the entanglement of the severed sheath in the aortic non-coronary cusp which allowed for its entrapment by the AL2 catheter. This allowed for the coronary guidewire BMW Universal (0.014”) placement and a slow balloon retrieval (SeQuent NEO 2.5 x 25 mm) of the severed sheath into the introducer sheath. The guidewire/balloon catheter was then exchanged for the support wire (0.035”) followed by the removal of the introducer sheath, AL2 catheter and the severed sheath. In conclusion, sheath severing is a complex accidental event during TAVR, which can be solved by intra-aortic recapture and retraction.

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