scholarly journals Genome-Wide Selective Signature Analysis Revealed Insecticide Resistance Mechanisms in Cydia pomonella

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 2
Author(s):  
Wen-Ting Dai ◽  
Jin Li ◽  
Li-Ping Ban
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Glycine Receptor ◽  
Cydia Pomonella ◽  
Resistance Management ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
Expression Level ◽  
Invasive Pest ◽  
Cross Resistance

The codling moth, Cydia pomonella L. (Lepidoptera, Tortricidae), is a serious invasive pest of pome fruits. Currently, C. pomonella management mainly relies on the application of insecticides, which have driven the development of resistance in the insect. Understanding the genetic mechanisms of insecticide resistance is of great significance for developing new pest resistance management techniques and formulating effective resistance management strategies. Using existing genome resequencing data, we performed selective sweep analysis by comparing two resistant strains and one susceptible strain of the insect pest and identified seven genes, among which, two (glycine receptor and glutamate receptor) were under strong insecticide selection, suggesting their functional importance in insecticide resistance. We also found that eight genes including CYP6B2, CYP307a1, 5-hydroxytryptamine receptor, cuticle protein, and acetylcholinesterase, are potentially involved in cross-resistance to azinphos-methyl and deltamethrin. Moreover, among several P450s identified as positively selected genes, CYP6B2, CYP4C1, and CYP4d2 showed the highest expression level in larva compared to other stages tested, and CYP6B2 also showed the highest expression level in midgut, supporting the roles they may play in insecticide metabolism. Our results provide several potential genes that can be studied further to advance understanding of complexity of insecticide resistance mechanisms in C. pomonella.

Section 4. Pyrethroid and endosulfan resistance: selection and cross resistance studies

1993 ◽  
Vol 1 ◽  
pp. 28-35 ◽  
Author(s):  
Neil W. Forrester ◽  
Matthew Cahill ◽  
Lisa J. Bird ◽  
Jacquelyn K. Layland
Keyword(s):  
Insecticide Resistance ◽  
Lower Order ◽  
Management Strategy ◽  
Pyrethroid Resistance ◽  
Resistance Management ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Male And Female ◽  
Insecticide Resistance Management ◽  
Resistance Selection

SummaryResistance to endosulfan and pyrethroids in Helicoverpa armigera in Australia was shown to be due to multiple rather than cross resistance. The independence of the endosulfan and pyrethroid resistance mechanisms vindicates the sequential use of these two groups in Stages I and II of the insecticide resistance management strategy, respectively. Within the cyclodienes, greatest resistance occurred to dieldrin with lower order cross resistance to endosulfan and endrin. Male and female moths expressed cyclodiene resistance equally.

scholarly journals Cross-Resistance of the Codling Moth against Different Isolates of Cydia pomonella Granulovirus Is Caused by Two Different but Genetically Linked Resistance Mechanisms

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1952
Author(s):  
Annette J. Sauer ◽  
Eva Fritsch ◽  
Karin Undorf-Spahn ◽  
Kento Iwata ◽  
Regina G. Kleespies ◽  
...  
Keyword(s):  
Biological Control Agent ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
Cross Resistance ◽  
Viral Gene ◽  
Type I ◽  
Type Ii ◽  
Cydia Pomonella Granulovirus ◽  
Type Ii Resistance

Cydia pomonella granulovirus (CpGV) is a widely used biological control agent of the codling moth. Recently, however, the codling moth has developed different types of field resistance against CpGV isolates. Whereas type I resistance is Z chromosomal inherited and targeted at the viral gene pe38 of isolate CpGV-M, type II resistance is autosomal inherited and targeted against isolates CpGV-M and CpGV-S. Here, we report that mixtures of CpGV-M and CpGV-S fail to break type II resistance and is expressed at all larval stages. Budded virus (BV) injection experiments circumventing initial midgut infection provided evidence that resistance against CpGV-S is midgut-related, though fluorescence dequenching assay using rhodamine-18 labeled occlusion derived viruses (ODV) could not fully elucidate whether the receptor binding or an intracellular midgut factor is involved. From our peroral and intra-hemocoel infection experiments, we conclude that two different (but genetically linked) resistance mechanisms are responsible for type II resistance in the codling moth: resistance against CpGV-M is systemic whereas a second and/or additional resistance mechanism against CpGV-S is located in the midgut of CpR5M larvae.

Investigation of insecticide-resistance status of Cydia pomonella in Chinese populations

2015 ◽  
Vol 105 (3) ◽  
pp. 316-325 ◽  
Author(s):  
X.-Q. Yang ◽  
Y.-L. Zhang
Keyword(s):  
Insecticide Resistance ◽  
Cydia Pomonella ◽  
Resistance Mutation ◽  
Codling Moth ◽  
Northwestern China ◽  
Invasive Pest ◽  
Native Page ◽  
Chinese Populations ◽  
Care Activity ◽  
Chlorpyrifos Ethyl

AbstractThe codling moth Cydia pomonella (L.) is an economically important fruit pest and it has been directly targeted by insecticides worldwide. Serious resistance to insecticides has been reported in many countries. As one of the most serious invasive pest, the codling moth has populated several areas in China. However, resistance to insecticides has not been reported in China. We investigated the insecticide-resistance status of four field populations from Northwestern China by applying bioassays, enzyme activities, and mutation detections. Diagnostic concentrations of lambda-cyhalothrin, chlorpyrifos-ethyl, carbaryl, and imidacloprid were determined and used in bioassays. Field populations were less susceptible to chlorpyrifos-ethyl and carbaryl than laboratory strain. Insensitive populations displayed an elevated glutathione S-transferases (GSTs) activity. Reduced carboxylesterase (CarE) activity was observed in some insecticide insensitive populations and reduced acetylcholinesterase activity was observed only in the Wuw population. The cytochrome P450 polysubstrate monooxygenases activities in four field populations were not found to be different from susceptible strains. Neither the known-resistance mutation F399V in the acetylcholinesterase (AChE) gene, ace1, nor mutations in CarE gene CpCE-1 were found in adult individuals from our field populations. Native-PAGE revealed that various CarE isozymes and AChE insensitivity were occurring among Chinese populations. Our results indicate that codling moth populations from Northwestern China were insensitivity to chlorpyrifos-ethyl and carbaryl. Increased GST activity was responsible for insecticides insensitivity. Decreased CarE activity, as well as the presence of CarE and AChE polymorphisms might also be involved in insecticides insensitivity. New management strategies for managing this pest are discussed.

Diversity of insecticide resistance mechanisms and spectrum in European populations of the codling moth,Cydia pomonella

2007 ◽  
Vol 63 (9) ◽  
pp. 890-902 ◽  
Author(s):  
Maritza Reyes ◽  
Pierre Franck ◽  
Pierre-Joseph Charmillot ◽  
Claudio Ioriatti ◽  
Jérôme Olivares ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
European Populations

scholarly journals A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Fanghao Wan ◽  
Chuanlin Yin ◽  
Rui Tang ◽  
Maohua Chen ◽  
Qiang Wu ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Genome Assembly ◽  
Association Studies ◽  
Receptor Gene ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Invasive Pest ◽  
Z Chromosome ◽  
Genome Wide Association Studies ◽  
Chromosome Level

Abstract The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.

scholarly journals Pest Management Challenges and Control Practices in Codling Moth: A Review

Insects ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Martina Kadoić Balaško ◽  
Renata Bažok ◽  
Katarina M. Mikac ◽  
Darija Lemic ◽  
Ivana Pajač Živković
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Cydia Pomonella ◽  
Genetic Research ◽  
Codling Moth ◽  
Fruit Production ◽  
Control Measures ◽  
Effective Control ◽  
Pome Fruit ◽  
Proactive Management

The codling moth, Cydia pomonella L., is a serious insect pest in pome fruit production worldwide with a preference for apple. The pest is known for having developed resistance to several chemical groups of insecticides, making its control difficult. The control and management of the codling moth is often hindered by a lack of understanding about its biology and ecology, including aspects of its population genetics. This review summarizes the information about the origin and biology of the codling moth, describes the mechanisms of resistance in this pest, and provides an overview of current research of resistant pest populations and genetic research both in Europe and globally. The main focus of this review is on non-pesticide control measures and anti-resistance strategies which help to reduce the number of chemical pesticides used and their residues on food and the local environment. Regular monitoring for insecticide resistance is essential for proactive management to mitigate potential insecticide resistance. Here we describe techniques for the detection of resistant variants and possibilities for monitoring resistance populations. Also, we present our present work on developing new methods to maintain effective control using appropriate integrated resistance management (IRM) strategies for this economically important perennial pest.

scholarly journals Monthly Weather Forecasts in a Pest Forecasting Context: Downscaling, Recalibration, and Skill Improvement

2012 ◽  
Vol 51 (9) ◽  
pp. 1633-1638 ◽  
Author(s):  
Martin Hirschi ◽  
Christoph Spirig ◽  
Andreas P. Weigel ◽  
Pierluigi Calanca ◽  
Jörg Samietz ◽  
...  
Keyword(s):  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Lead Times ◽  
Practical Applications ◽  
Weather Forecasts ◽  
Weather Information ◽  
Agricultural Applications ◽  
Resampling Procedure ◽  
Skill Improvement

AbstractMonthly weather forecasts (MOFCs) were shown to have skill in extratropical continental regions for lead times up to 3 weeks, in particular for temperature and if weekly averaged. This skill could be exploited in practical applications for implementations exhibiting some degree of memory or inertia toward meteorological drivers, potentially even for longer lead times. Many agricultural applications fall into these categories because of the temperature-dependent development of biological organisms, allowing simulations that are based on temperature sums. Most such agricultural models require local weather information at daily or even hourly temporal resolution, however, preventing direct use of the spatially and temporally aggregated information of MOFCs, which may furthermore be subject to significant biases. By the example of forecasting the timing of life-phase occurrences of the codling moth (Cydia pomonella), which is a major insect pest in apple orchards worldwide, the authors investigate the application of downscaled weekly temperature anomalies of MOFCs for use in an impact model requiring hourly input. The downscaling and postprocessing included the use of a daily weather generator and a resampling procedure for creating hourly weather series and the application of a recalibration technique to correct for the original underconfidence of the forecast occurrences of codling moth life phases. Results show a clear skill improvement of up to 3 days in root-mean-square error over the full forecast range when incorporating MOFCs as compared with deterministic benchmark forecasts using climatological information for predicting the timing of codling moth life phases.

Identification of a point mutation in the ace1 gene of Therioaphis trifolli maculata and detection of insecticide resistance by a diagnostic PCR–RFLP assay

2015 ◽  
Vol 105 (6) ◽  
pp. 712-716
Author(s):  
E. AlSuhaibani ◽  
C.C. Voudouris ◽  
R. Al-Atiyat ◽  
A. Kotzamumin ◽  
J. Vontas ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Insecticide Resistance ◽  
Management Strategies ◽  
Resistance Management ◽  
Resistance Mutation ◽  
Resistance Mechanisms ◽  
Effective Control ◽  
Agricultural Pests ◽  
Resistance Mutations ◽  
Diagnostic Pcr

AbstractAphids are important agricultural pests worldwide. Their control is largely based on chemical insecticides. One species that shows important invasive abilities and host-plant-related differences is Therioaphis trifolii (Monell) (Hemiptera: Aphididae). T. trifolii maculata, also known as spotted alfalfa aphid (SAA), can be very injurious to alfalfa crops in certain regions, such as in Saudi Arabia for effective control it is essential to diagnose and monitor the resistance mechanisms in the SAA populations. In the present study, we analysed acetylcholinesterase (ace) target site insensitivity mechanisms. A 650 bp length DNA containing the putative acetylcholinesterase (ace1) precursor was obtained and compared with other Hemipteran species. The sequences of many individual aphids collected from alfalfa crops in Saudi Arabia were analysed for the presence of resistance mutations: no resistance mutations were found at the resistance mutation loci 302; however, the presence of a serine–phenylalanine substitution (S431F) was identified in one individual. The S431F substitution, has been shown to confer significant levels of both organophosphate and carbamate resistance in other aphid species, and is now found for the first time in T. trifolii. We subsequently developed a simple polymerase chain reaction–restriction fragment length polymorphism assays for the S431F mutation, using a TaqI restriction site destroyed by the S431F mutation. The novel diagnostic assay may support the implementation of Insecticide Resistance Management strategies, for the control of SAA in alfalfa crops in the Kingdom of Saudi Arabia, and other countries worldwide.

Evidence for resistance to pyrethroids and organophosphates inPlutella xylostella(Lepidoptera: Plutellidae) from Pakistan

2007 ◽  
Vol 97 (2) ◽  
pp. 191-200 ◽  
Author(s):  
A. Khaliq ◽  
M.N.R. Attique ◽  
A.H. Sayyed
Keyword(s):  
Insecticide Resistance ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Plutella Xylostella ◽  
Management Strategies ◽  
Resistance Management ◽  
Resistance Mechanisms ◽  
Laboratory Population ◽  
Multiple Resistance ◽  
Low Toxicity

AbstractThe susceptibility of representative pyrethroid (cypermethrin, deltamethrin, lambdacyhalothrin, bifenthrin), organophosphate (chlorpyriphos, triazophos, profenophos) and new chemistry insecticides (spinosad, indoxacarb and emamectin) was investigated for 18 field populations ofPlutella xylostella(Linnaeus) from three different zones in Pakistan. The LC50(mg ml−1; 48 h) values of pyrethroids for various populations ranged from 0.19–1.88 for cypermethrin, 0.31–2.64 for deltamethrin, 0.08–1.16 for lambdacyhalothrin and 0.07–0.88 for bifenthrin. The LC50(mg ml−1; 48 h) of organophosphates ranged from 0.52–5.67 for chlorpyriphos, 0.37–4.14 for triazophos and 0.03–2.65 for profenophos. The most probable reason for low toxicity of organophosphates and pyrethroids is the evolution of multiple resistance mechanisms; however, further studies are required to establish these mechanisms. When these same products were tested against a susceptible laboratory population (Lab-Pak), the new chemistry compounds were significantly more toxic than pyrethroids and organophosphates. The results are discussed in relation to integrated pest management and insecticide resistance management strategies forP. xylostella.

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