scholarly journals Use of Pyriproxyfen to Induce Oogenesis in Diapausing Megacopta cribraria (Heteroptera: Plataspidae), and Evaluation of Pyriproxyfen-Induced Eggs for Rearing the Parasitoid Paratelenomus saccharalis (Hymenoptera: Scelionidae)

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 89
Author(s):  
Cory Penca ◽  
Nicholas C. Goltz ◽  
Amanda C. Hodges ◽  
Norman C. Leppla ◽  
Joseph E. Eger ◽  
...  
Keyword(s):  
Egg Production ◽  
Mass Rearing ◽  
Egg Parasitoid ◽  
General Applicability ◽  
Treatment Regimes ◽  
Long Day ◽  
Hormone Analog ◽  
Host Mortality ◽  
Abundant Supply ◽  
Parasitoid Rearing

The mass rearing of hymenopteran egg parasitoids requires an abundant supply of host eggs. The onset of reproductive diapause and subsequent decline in egg production poses a challenge for parasitoid rearing when using host colonies augmented by field-collected insects. We investigated the application of pyriproxyfen, a juvenile hormone analog, to induce oviposition in diapausing adult kudzu bugs, Megacopta cribraria (Fabricius) (Heteroptera: Plataspidae), and the use of eggs produced by pyriproxyfen-treated kudzu bugs to rear the egg parasitoid, Paratelenomus saccharalis (Dodd) (Hymenoptera: Scelionidae). The effects of pyriproxyfen and photoperiod treatments on host mortality, egg production, and rates of parasitoid eclosion from the eggs were used to calculate the parasitoid yield for the different treatment regimes. A combination of pyriproxyfen and a long-day photoperiod increased the parasitoid yield by 87% compared to acetone and a long-day photoperiod. The general applicability of JH-analog mediated egg production for parasitoid rearing is discussed.

scholarly journals Integrative taxonomy and phylogeography of Telenomus remus (Scelionidae), with the first record of natural parasitism of Spodoptera spp. in Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana P. G. S. Wengrat ◽  
Aloisio Coelho Junior ◽  
Jose R. P. Parra ◽  
Tamara A. Takahashi ◽  
Luis A. Foerster ◽  
...  
Keyword(s):  
Biological Control ◽  
Mitochondrial Gene ◽  
First Record ◽  
Mass Rearing ◽  
Classical Biological Control ◽  
Egg Parasitoid ◽  
Integrative Approach ◽  
Agricultural Pests ◽  
Telenomus Remus ◽  
Natural Parasitism

AbstractThe egg parasitoid Telenomus remus (Hymenoptera: Scelionidae) has been investigated for classical and applied biological control of noctuid pests, especially Spodoptera (Lepidoptera: Noctuidae) species. Although T. remus was introduced into Brazil over three decades ago for classical biological control of S. frugiperda, this wasp has not been recorded as established in corn or soybean crops. We used an integrative approach to identify T. remus, combining a taxonomic key based on the male genitalia with DNA barcoding, using a cytochrome c oxidase subunit I mitochondrial gene fragment. This is the first report of natural parasitism of T. remus on S. frugiperda and S. cosmioides eggs at two locations in Brazil. We also confirmed that the T. remus lineage in Brazil derives from a strain in Venezuela (originally from Papua New Guinea and introduced into the Americas, Africa, and Asia). The occurrence of T. remus parasitizing S. frugiperda and S. cosmioides eggs in field conditions, not associated with inundative releases, suggests that the species has managed to establish itself in the field in Brazil. This opens possibilities for future biological control programs, since T. remus shows good potential for mass rearing and egg parasitism of important agricultural pests such as Spodoptera species.

scholarly journals Cold storage and gamma irradiation of Sitotroga cerealella Olivier eggs (Lepidoptera: Gelechiidae) in relation to the success of parasitism by Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae)

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Naglaa F. Abdel-Hameid ◽  
I. R. M. Elzoghby ◽  
A. L. Mehany ◽  
W. A. A. Sayed
Keyword(s):  
Gamma Irradiation ◽  
Cold Storage ◽  
Mass Rearing ◽  
Egg Parasitoid ◽  
Sitotroga Cerealella ◽  
Trichogramma Evanescens ◽  
Control Programs ◽  
Angoumois Grain Moth ◽  
Rearing Techniques ◽  
The Times

AbstractThe performance of parasitism by the egg parasitoid, Trichogramma evanescens Westwood (Hymenoptera: Trichogrammatidae) on eggs of Angoumois grain moth, Sitotroga cerealella Olivier (Lepidoptera: Gelechiidae) was investigated under cold storage and gamma irradiation treatments of the host eggs. Cold storage treatment could improve the parasitoid mass rearing techniques and reduced the costs of biological control programs, while gamma irradiation might be used as a supplementary support at the times of high demand. The suitability of the S. cerealella eggs, stored at – 20 °C for 0.5, 1, or 2 h. as a host for T. evanescens was evaluated. The sensitivity of S. cerealella eggs to gamma irradiation treatments and the acceptability of irradiated eggs for parasitism by T. evanescens females for the parental P and F1 generations were examined. The results revealed that parasitism was drastically reduced more than adult’s emergence and sex-ratio (% of females) after cold storage periods of S. cerealella eggs. Moreover, the parasitism percentages were relatively reduced to (97.1, 96.1, 93.03, and 92.7 %) after irradiating the S. cerealella eggs at 40, 60, 80, and 100 Gy, respectively than the control (97.3% emergence). The percentages of emergence and females’ percent were slightly decreased by gamma irradiation doses, while, equal preferred by the F1 generation of parasitoid that produced from irradiated S. cerealella eggs.

The population dynamics of metacercariae of Apatemon gracilis (Trematoda: Digenea) in three species of lake-dwelling leeches

Parasitology ◽  
1986 ◽  
Vol 93 (3) ◽  
pp. 517-530 ◽  
Author(s):  
S. M. Spelling ◽  
J. O. Young
Keyword(s):  
Population Dynamics ◽  
Relative Density ◽  
Sexual Maturity ◽  
Egg Production ◽  
Seasonal Pattern ◽  
Distinct Pattern ◽  
The Other ◽  
Mean Intensity ◽  
Density Values ◽  
Host Mortality

SUMMARYMonthly samples of the leeches Erpobdella octoculata, Glossiphonia complanata and Helobdella stagnalis were taken over a two-year period from an eutrophic, English lake to detect metacercariae of the trematode, Apatemon gracilis. In each cohort of each of the three leeches, prevalence was low in young individuals, rose to a peak in autumn/winter, and then declined until the cohort had almost died out; in E. octoculata and H. stagnalis a final brief increase occurred. Mean intensity and relative density values followed a similar seasonal pattern of change to that of prevalence in these last two species, but in G. complanata values fluctuated irregularly with no distinct pattern. The frequency distribution of the parasite in G. complanata was highly over-dispersed, but less so in the other two species. Infected E. octoculata reached sexual maturity. The parasite reduced egg production in G. complanata and H. stagnalis, but only by maximum values of 2·5 and 9% respectively. This reduction in fecundity is low compared to the subsequent high mortality, at 95% or more, of newly recruited young from as yet unidentified causes. Parasite-related host mortality was difficult to assess in young leeches, but there was some evidence for its occurrence in older leeches of E. octoculata and H. stagnalis. However, this is unlikely to play a prominent role in the control and regulation of lacustrine leech populations.

scholarly journals Implementing Mass Rearing of Trissolcus japonicus (Hymenoptera: Scelionidae) on Cold-Stored Host Eggs

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 840
Author(s):  
Barbara Bittau ◽  
Maria Luisa Dindo ◽  
Giovanni Burgio ◽  
Giuseppino Sabbatini-Peverieri ◽  
Kim Alan Hoelmer ◽  
...  
Keyword(s):  
Mass Rearing ◽  
Classical Biological Control ◽  
Egg Parasitoid ◽  
Egg Mass ◽  
Severe Damage ◽  
Progeny Production ◽  
Asian Origin ◽  
Egg Masses ◽  
Trissolcus Japonicus ◽  
Promising Solution

Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), a pest of Asian origin, has been causing severe damage to Italian agriculture. The application of classical biological control by the release of Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae), an exotic egg parasitoid, appears to be one promising solution. In Italy, releases of T. japonicus in the field were authorized in 2020. In this study, some parameters that could influence the rearing of T. japonicus in insectaries were investigated. A significantly higher production of progeny was observed on host eggs stored at 6 °C (86.5%) compared to −24 °C (48.8%) for up to two months prior to exposure to parasitism. There were no significant differences in progeny production from single females in a vial provided with only one egg mass (83.2%) or 10 females inside a cage with 6 egg masses (83.9%). The exposure of parasitoids to refrigerated (6 °C) egg masses of H. halys for 72 h led to a significantly higher production of progeny (62.1%) compared to shorter exposures for 48 (44.0%) or 24 h (37.1%). A decline in production of progeny by the same female was detected between the first (62.1%) and the second parasitization (41.3%). Adult parasitoids stored at 16 °C for up to 90 days had an 87.1% survival rate, but a significant decrease in progeny production was detected. These parameters could be adjusted when rearing T. japonicus for specific aims such as the production of individuals for field release or colony maintenance.

3.5 SUPPRESSION OF SPRUCE BUDWORM POPULATIONS BY TRICHOGRAMMA MINUTUM RILEY, 1982–1986

1990 ◽  
Vol 122 (S153) ◽  
pp. 56-81 ◽  
Author(s):  
S.M. Smith ◽  
D.R. Wallace ◽  
G. Howse ◽  
J. Meating
Keyword(s):  
Choristoneura Fumiferana ◽  
Host Density ◽  
Spruce Budworm ◽  
Mass Rearing ◽  
Egg Parasitoid ◽  
Curvilinear Relationship ◽  
Egg Masses ◽  
Trichogramma Minutum ◽  
Egg Parasitism ◽  
Release Timing

AbstractThe ability of the egg parasitoid, Trichogramma minutum Riley, to suppress outbreak populations of the spruce budworm, Choristoneura fumiferana (Clemens), was studied annually near Hearst, Ont., from 1982 through 1986. Timing of broadcast parasitoid-releases was linked to spruce budworm moth emergence and oviposition. These phenological relationships were predicted from a regression based on larval development at least 2 weeks before expected emergence; this allowed sufficient time to regulate (program) parasitoid emergence during mass-rearing. Emergence of caged spruce budworm adults was used to monitor moth eclosion in the field. Pheromone traps provided daily information on the activity of male moths and helped to synchronize the parasitoid releases with spruce budworm oviposition. Information on parasitoid activity was obtained from sentinel (laboratory-reared) and naturally occurring spruce budworm egg masses. A curvilinear relationship between the rate of parasitoid release and parasitism of sentinel egg masses was developed. Two parasitoid releases, 1 week apart, early in the oviposition period of spruce budworm, significantly increased parasitism of host eggs by 14–83% and reduced larval populations correspondingly from 42 to 82%. Single releases were less effective and increased parasitism by 0.3–52% (single ground release, 1986). Two parasitoid releases, combined with a spring application of Bacillus thuringiensis Berliner to larval populations, was the most effective strategy and resulted in 83% egg parasitism and 93% larval reduction. Release rates greater than 12–16 × 106 ♀ ♀ T. minutum per hectare were not warranted based on impact and costs. The effects of release timing, weather, host density, and parasitoid quality on the future successful use of T. minutum are discussed.

When Less Is More: Sex Ratios for the Mass-Rearing of Anastrepha ludens (Diptera: Tephritidae)

2019 ◽  
Author(s):  
G I González-López ◽  
E Solís-Echeverría ◽  
F Díaz-Fleischer ◽  
D Pérez-Staples
Keyword(s):  
Sex Ratio ◽  
Egg Production ◽  
Sex Ratios ◽  
Pupal Stage ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Adult Density ◽  
Genetic Sexing ◽  
Male Harassment ◽  
Less Is More

Abstract Anastrepha ludens (Loew) is one of the most important pests of citrus and mango crops in Mexico. A method used to control this pest is the sterile insect technique, which consists in the mass production, irradiation, and release of insects in affected areas. The production of insects begins with the establishment of colonies to produce eggs, which must be highly fertile to ensure an adequate production of larvae. However, female fecundity and fertility can be affected by adult density and sex ratio, thus an optimal sex ratio in mass-rearing cages must be used. The genetic sexing strain of A. ludens (Tapachula-7) allows the identification of the sex at the pupal stage, making it possible to establish rearing cages with different sex ratios. We determined if different sex ratios have an effect on egg production. Two sex ratios (4♀: 1♂ and 1♀: 1♂) were compared. Fecundity, fertility and survival at different ages were also determined. Higher fertility and fecundity per female were observed at a ratio of 4:1. However, females with higher fecundity had reduced survival probabilities. In conclusion, maintaining colonies with a lower proportion of males in cages ensures a greater fecundity and fertility. Further research is necessary to understand whether results can be attributed to lower male harassment in cages.

Study on different diets on the biological parameters of rice moth Corcyra cephalonica (Stainton)

2019 ◽  
Vol 4 (01) ◽  
pp. 49-54
Author(s):  
Ravi Kumar ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Joginder Singh ◽  
Ankit Kumar ◽  
...  
Keyword(s):  
Egg Production ◽  
Egg Parasitoid ◽  
Corcyra Cephalonica ◽  
Biological Parameters ◽  
College Of Agriculture ◽  
Trichogramma Chilonis ◽  
Effective Performance ◽  
Agricultural Entomology ◽  
Yeast Powder ◽  
Factitious Host

The present investigations were undertaken in laboratory on “Study on factitious host Corcyra cephalonica (Stainton) against storage of egg parasitoid Trichogramma chilonis (Ishii) cards under laboratory conditions” during the year 2018 in the bio-control laboratory, Department of Agricultural Entomology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U. P.) India. The effects of different rearing media on some biological parameters of factitious host, C. cephalonica are based on results are most effective performance with T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), treatments i.e. larval, pupal, male and female moths emergence, and egg production in cc and followed by 17530, 16667, 15131,14875, 13545 and 11830 i.e. T3 (Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast), T5 (Sorghum + Soyabeen + Yeast powder, 50:50 + 5g Yeast), T6 (Sorghum + Cowpea + Yeast powder, 50:50 + 5g Yeast), T4 (Sorghum + Gram powder + Yeast powder, 50:50 + 5g Yeast), T2 (Sorghum + Green Gram + Yeast powder, 50:50 +5g Yeast), T1 (Sorghum + Black gram + Yeast powder, 50:50 + 5g Yeast) and T7 (Sorghum alone (check) eggs per cc of Sorghum + Groundnut + Yeast powder, 50:50 + 5g Yeast and wheal), respectively.

Yeast derivatives and wheat germ in the adult diet modulates fecundity in a tephritid pest

2018 ◽  
Vol 109 (2) ◽  
pp. 178-190 ◽  
Author(s):  
L. Goane ◽  
P.M. Pereyra ◽  
F. Castro ◽  
M.J. Ruiz ◽  
M.L. Juárez ◽  
...  
Keyword(s):  
Egg Production ◽  
Wheat Germ ◽  
Low Cost ◽  
Nutrient Content ◽  
Mass Rearing ◽  
Protein Source ◽  
Brewer’S Yeast ◽  
Brewer's Yeast ◽  
Adult Diet ◽  
Ovary Maturation

AbstractAnastrepha fraterculus (Wiedemann), a pest of great economic importance in South America, needs urgently to be controlled by environmentally friendly methods such as the sterile insect technique for which mass rearing of insects is required. Because oogenesis takes place during the adult stage, mass-rearing facilities should provide the females a diet that maximizes egg production at the lowest cost. Accordingly, we investigated the effect of artificial protein sources in the adult diet (yeast derivatives of different cost but with similar amino acids profiles, and the addition of wheat germ) on fecundity. Additionally, we evaluated different ratios of yeast derivatives or wheat germ on ovary maturation, fecundity, and fertility as well as their association with the nutrient content of females. Females fed hydrolyzed yeast and yeast extract attained the highest fecundity level, and those fed brewer's yeast the lowest. Reducing the amount of hydrolyzed yeast, an expensive protein source, in the diet negatively affected fecundity and ovary maturation. Increasing the amount of brewer's yeast, a low-cost protein source, did not favor fecundity. The addition of wheat germ in the adult diet improved fecundity regardless of the yeast derivate considered. Percentage of egg hatch was not affected by the diet. Nutrient content of A. fraterculus females varied according to the adult diet provided and mating status. Our findings provide novel baseline information to understand the role of nutrition on reproductive performance of A. fraterculus females and are discussed in the context of resource allocation. They also provide valuable advances in the search for cost-effective adult diets at fruit fly mass rearing facilities.

scholarly journals KESESUAIAN TELUR KEPIK KEDELAI UNTUK PEMBIAKAN MASSAL Anastatus dasyni FERR. (HYMENOPTERA: EUPELMIDAE), PARASITOID TELUR KEPIK LADA

2020 ◽  
Vol 16 (3) ◽  
pp. 119
Author(s):  
IWA .M. TRISAWA ◽  
A. RAUF ◽  
U. KARTOSUWONDO ◽  
N. MARYANA ◽  
A. NURMANSYAH
Keyword(s):  
Intrinsic Rate ◽  
Mass Rearing ◽  
Egg Parasitoid ◽  
Reproductive Rate ◽  
Nezara Viridula ◽  
Intrinsic Rate Of Increase ◽  
Pupal Development ◽  
Net Reproductive Rate ◽  
Immature Development ◽  
Rate Of Increase

<p>ABSTRAK</p><p>Anastatus dasyni Ferr. adalah parasitoid telur kepik lada, Dasynuspiperis China. Penelitian bertujuan mengkaji kesesuaian telur kepikkedelai Riptortus linearis dan Nezara viridula sebagai inang untukpembiakan massal parasitoid A. dasyni. Imago parasitoid A. dasyni yangberasal dari lapangan dipelihara secara terpisah pada telur dari kedua jeniskepik kedelai. Pengamatan dilakukan terhadap biologi A. dasyni yangmeliputi masa perkembangan pradewasa dan berbagai parameter kehi-dupan imago betina. Selain itu, dilakukan analisis neraca hayati denganmenggabungkan data perkembangan dan sintasan pradewasa, masa hidupimago dan reproduksi, serta nisbah kelamin. Hasil penelitian menunjukkanbahwa masa perkembangan larva dan pupa A dasyni pada telur N. viridulalebih singkat serta laju peneluran lebih tinggi dibandingkan pada telur R.linearis. Imago betina A. dasyni yang keluar dari telur N. viridula hanya1,81%, sedangkan dari telur R. linearis sebanyak 70,20%. Oleh karena itu,parameter neraca hayati hanya dapat dihitung dari parasitoid yangdipelihara pada telur R. linearis. Laju pertambahan intrinsik parasitoidadalah (r) 0,1870, masa generasi (T) 27,51 hari, reproduksi bersih (Ro)84,29, laju pertambahan terbatas (λ) 1,21, dan nilai reproduksi (RVx)402,51. Proporsi persebaran usia stabil (px) adalah 17,06% telur, 50,41%larva, 26,53% pupa, dan 6,02% imago. Telur kepik kedelai R. linearisdapat digunakan untuk pembiakan massal A. dasyni.</p><p>Kata kunci : Anastatus dasyni, Dasynus piperis, Riptortus linearis,Nezara viridula, parasitoid, pembiakan massal</p><p>ABSTRACT</p><p>Suitability of soybean bug eggs for mass rearing ofAnastatus dasyni Ferr. (Hymenoptera: Eupelmidae), anegg parasitoid of pepper bug</p><p>Anastatus dasyni Ferr. is an important egg parasitoid of pepper bug,Dasynus piperis China. Research was conducted with the objectives tostudy the suitability of eggs of soybean bugs Riptortus linearis and Nezaraviridula for mass rearing of A. dasyni. Adults of A. dasyni collected fromthe field were inoculated separately on eggs of two species of soybeanbugs. Biological parameters such as immature development and adultlongevity were observed daily. Lifetable parameters were calculated on thebasis of development and survival of immature, reproduction, and sexratio. Our studies revealed that parasitoid developing on Nezara viridulaeggs had shorter larval and pupal development and higher in ovipositionrate than those on Riptortus linearis eggs. However, parasitoids emergedfrom N. viridula eggs only 1.81% were females, while from R. lineariseggs were 70.20%. Therefore, lifetable parameters can only be generatedfrom parasitoid reared on R. linearis eggs. The intrinsic rate of increase (r)mean generation time (T) 0.1870, 27.51 days, 84.29, 1.21, and 402.51respectively net reproductive rate (Ro), finite rate of increase (λ), andreproductive value (RVx). The stable stage distribution (px) were 17,06%eggs, 50,41% larvae, 26,53% pupae, and 6,02% adults. Eggs of soybeanbug R. linearis can be used for mass rearing of parasitoid A. dasyni.</p><p>Key words : Anastatus dasyni, Dasynus piperis, Riptortus linearis,Nezara viridula, parasitoid, mass rearing</p>

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