scholarly journals Tissue Engineering and Regeneration of the Human Hair Follicle in Androgenetic Alopecia: Literature Review

Life ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 117
Author(s):  
José María Llamas-Molina ◽  
Alejandro Carrero-Castaño ◽  
Ricardo Ruiz-Villaverde ◽  
Antonio Campos
Keyword(s):  
Tissue Engineering ◽  
Hair Follicle ◽  
Hair Follicles ◽  
Androgenetic Alopecia ◽  
Future Prospects ◽  
Oral Drugs ◽  
Human Hair Follicle ◽  
Advantages And Disadvantages ◽  
Discontinuation Of Treatment ◽  
Bioengineering Techniques

Androgenetic alopecia (AGA) is an androgen-dependent process and represents the most frequent non-scarring alopecia. Treatments for AGA do not always achieve a satisfactory result for the patient, and sometimes cause side effects that lead to discontinuation of treatment. AGA therapeutics currently includes topical and oral drugs, as well as follicular unit micro-transplantation techniques. Tissue engineering (TE) is postulated as one of the possible future solutions to the problem and aims to develop fully functional hair follicles that maintain their cyclic rhythm in a physiological manner. However, despite its great potential, reconstitution of fully functional hair follicles is still a challenge to overcome and the knowledge gained of the key processes in hair follicle morphogenesis and biology has not yet been translated into effective replacement therapies in clinical practice. To achieve this, it is necessary to research and develop new approaches, techniques and biomaterials. In this review, present and emerging hair follicle bioengineering strategies are evaluated. The current problems of these bioengineering techniques are discussed, as well as the advantages and disadvantages, and the future prospects for the field of TE and successful hair follicle regeneration.

A method for culturing human hair follicle cells. (II). Plucked human hair follicles.

1988 ◽  
Vol 50 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Ryuichiro KUWANA ◽  
Seiji ARASE ◽  
Yasushi SADAMOTO ◽  
Hideki NAKANISHI ◽  
Katsuyuki TAKEDA
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Hair Follicles ◽  
Follicle Cells ◽  
Human Hair Follicle

scholarly journals Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle

2021 ◽  
Author(s):  
Megan A. Palmer ◽  
Eleanor Smart ◽  
Iain S. Haslam
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Vital Role ◽  
Transport Proteins ◽  
Hair Follicles ◽  
Regulatory Control ◽  
Cholesterol Transport ◽  
Hair Cycle ◽  
Human Hair Follicle

AbstractCholesterol has long been suspected of influencing hair biology, with dysregulated homeostasis implicated in several disorders of hair growth and cycling. Cholesterol transport proteins play a vital role in the control of cellular cholesterol levels and compartmentalisation. This research aimed to determine the cellular localisation, transport capability and regulatory control of cholesterol transport proteins across the hair cycle. Immunofluorescence microscopy in human hair follicle sections revealed differential expression of ATP-binding cassette (ABC) transporters across the hair cycle. Cholesterol transporter expression (ABCA1, ABCG1, ABCA5 and SCARB1) reduced as hair follicles transitioned from growth to regression. Staining for free cholesterol (filipin) revealed prominent cholesterol striations within the basement membrane of the hair bulb. Liver X receptor agonism demonstrated active regulation of ABCA1 and ABCG1, but not ABCA5 or SCARB1 in human hair follicles and primary keratinocytes. These results demonstrate the capacity of human hair follicles for cholesterol transport and trafficking. Future studies examining the role of cholesterol transport across the hair cycle may shed light on the role of lipid homeostasis in human hair disorders.

scholarly journals Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632

2021 ◽  
Vol 9 ◽  
Author(s):  
Lihong Wen ◽  
Yong Miao ◽  
Zhexiang Fan ◽  
Jiarui Zhang ◽  
Yixuan Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Culture System ◽  
Hair Follicles ◽  
Human Hair Follicle ◽  
Rock Inhibitor ◽  
Hair Follicle Stem Cells ◽  
Human Fibronectin ◽  
Follicle Stem Cells

BackgroundHair follicle tissue engineering is a promising strategy for treating hair loss. Human hair follicle stem cells (hHFSCs), which play a key role in the hair cycle, have potential applications in regenerative medicine. However, previous studies did not achieve efficient hHFSC expansion in vitro using feeder cells. Therefore, there is a need to develop an efficient primary culture system for the expansion and maintenance of hHFSCs.MethodsThe hHFSCs were obtained by two-step proteolytic digestion combined with microscopy. The cell culture dishes were coated with human fibronectin and inoculated with hHFSCs. The hHFSCs were harvested using a differential enrichment procedure. The effect of Rho-associated protein kinase (ROCK) inhibitor Y-27632, supplemented in keratinocyte serum-free medium (K-SFM), on adhesion, proliferation, and stemness of hHFSCs and the underlying molecular mechanisms were evaluated.ResultsThe hHFSCs cultured in K-SFM, supplemented with Y-27632, exhibited enhanced adhesion and proliferation. Additionally, Y-27632 treatment maintained the stemness of hHFSCs and promoted the ability of hHFSCs to regenerate hair follicles in vivo. However, Y-27632-induced proliferation and stemness in hHFSCs were conditional and reversible. Furthermore, Y-27632 maintained propagation and stemness of hHFSCs through the ERK/MAPK pathway.ConclusionAn efficient short-term culture system for primary hHFSCs was successfully established using human fibronectin and the ROCK inhibitor Y-27632, which promoted the proliferation, maintained the stemness of hHFSCs and promoted the ability to regenerate hair follicles in vivo. The xenofree culturing method used in this study provided a large number of high-quality seed cells, which have applications in hair follicle tissue engineering and stem cell therapy.

scholarly journals Plasticity of Rodent and Human Hair Follicle Dermal Cells:Implications for Cell Therapy and Tissue Engineering

2005 ◽  
Vol 10 (3) ◽  
pp. 180-183 ◽  
Author(s):  
Gavin D. Richardson ◽  
Elisabeth C. Arnott ◽  
Claire J. Whitehouse ◽  
Clifford M. Lawrence ◽  
Amanda J. Reynolds ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Therapy ◽  
Hair Follicle ◽  
Human Hair ◽  
Human Hair Follicle

scholarly journals DEMONSTRATION OF THE HUMAN HAIR FOLLICLE MAGNETORECEPTION OF BIOMAGNETISM RADIATED BY THE CONCAVE PART OF THE HUMAN HAND

2020 ◽  
Vol 8 (5) ◽  
pp. 348-354
Author(s):  
Abraham A. Embi
Keyword(s):  
Optical Microscopy ◽  
Hair Follicle ◽  
Human Hair ◽  
Quantum Interference ◽  
Hair Follicles ◽  
The Body ◽  
Human Hand ◽  
Human Hair Follicle ◽  
Concave Part ◽  
Microscopy Method

Biological material has been documented to produce an external magnetic field that radiates out. There have been several papers documenting the magnetic fields produced by steady currents in the body. The most notable was published in 1980 by Cohen et al. where the human hair follicle was used as sentinel and biophysically evaluated via sophisticated equipment such as a double planar Superconducting Quantum Interference Devices (SQUID). Most recently, in 2019 Cohen’s work was duplicated by Khan,S by also using double-planar gladiometers. Of interest to this manuscript is that since the introduction of anovel optical microscopy method in 2016 by Scherlag BJ et al is that numerous papers have been introduced in the literature now identifying intrinsic biomagnetic properties of the follicle such as penetration through glass barriers. In this manuscript, a concept of biomagnetic fields by the concave part of the human hand transferring energy to hair follicles is introduced, this was accomplished by using a novel optical microscopy method, in other words, the hair follicle is not limited to radiate out biomagnetism; but also, to receive externally radiated biomagnetic fields from a body part. This magneto receptive property is herein introduced.

Regular expression of hair follicle keratins as crucial factors in tissue engineering of hair follicles

2011 ◽  
Vol 21 (6) ◽  
pp. 189-189
Author(s):  
Walter Krugluger ◽  
Karl Stiefsohn ◽  
Claudia Moser ◽  
Karl Moser
Keyword(s):  
Tissue Engineering ◽  
Hair Follicle ◽  
Regular Expression ◽  
Hair Follicles

scholarly journals Sustained Human Hair Follicle Growth Ex Vivo in a Glycosaminoglycan Hydrogel Matrix

2019 ◽  
Vol 20 (7) ◽  
pp. 1741 ◽  
Author(s):  
Sandra Fernández-Martos ◽  
María Calvo-Sánchez ◽  
Karla García-Alonso ◽  
Begoña Castro ◽  
Bita Hashtroody ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Human Hair ◽  
Wound Repair ◽  
Ex Vivo ◽  
Hair Follicles ◽  
Stem Cell Markers ◽  
Follicle Growth ◽  
Human Hair Follicle ◽  
Hydrogel Matrix ◽  
Hair Follicle Growth

Glycosaminoglycans (GAGs) and associated proteoglycans have important functions in homeostatic maintenance and regenerative processes (e.g., wound repair) of the skin. However, little is known about the role of these molecules in the regulation of the hair follicle cycle. Here we report that growing human hair follicles ex vivo in a defined GAG hydrogel mimicking the dermal matrix strongly promotes sustained cell survival and maintenance of a highly proliferative phenotype in the hair bulb and suprabulbar regions. This significant effect is associated with the activation of WNT/β-catenin signaling targets (CCDN1, AXIN2) and with the expression of stem cell markers (CK15, CD34) and growth factors implicated in the telogen/anagen transition (TGFβ2, FGF10). As a whole, these results point to the dermal GAG matrix as an important component in the regulation of the human hair follicle growth cycle, and to GAG-based hydrogels as potentially relevant modulators of this process both in vitro and in vivo.

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