scholarly journals Study on the Technology of Monodisperse Droplets by a High-Throughput and Instant-Mixing Droplet Microfluidic System

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1263
Author(s):  
Rui Xu ◽  
Shijiao Zhao ◽  
Lei Nie ◽  
Changsheng Deng ◽  
Shaochang Hao ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Multiple Solutions ◽  
Silicone Oil ◽  
Microfluidic System ◽  
Mixed Solution ◽  
Internal Gelation ◽  
Factors Affecting ◽  
Mixing Performance ◽  
Monodisperse Droplets

In this study, we report a novel high-throughput and instant-mixing droplet microfluidic system that can prepare uniformly mixed monodisperse droplets at a flow rate of mL/min designed for rapid mixing between multiple solutions and the preparation of micro-/nanoparticles. The system is composed of a magneton micromixer and a T-junction microfluidic device. The magneton micromixer rapidly mixes multiple solutions uniformly through the rotation of the magneton, and the mixed solution is sheared into monodisperse droplets by the silicone oil in the T-junction microfluidic device. The optimal conditions of the preparation of monodisperse droplets for the system have been found and factors affecting droplet size are analyzed for correlation; for example, the structure of the T-junction microfluidic device, the rotation speed of the magneton, etc. At the same time, through the uniformity of the color of the mixed solution, the mixing performance of the system is quantitatively evaluated. Compared with mainstream micromixers on the market, the system has the best mixing performance. Finally, we used the system to simulate the internal gelation broth preparation of zirconium broth and uranium broth. The results show that the system is expected to realize the preparation of ceramic microspheres at room temperature without cooling by the internal gelation process.

Step-emulsification in a microfluidic device

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1023-1031 ◽  
Author(s):  
Z. Li ◽  
A. M. Leshansky ◽  
L. M. Pismen ◽  
P. Tabeling
Keyword(s):  
Experimental Study ◽  
Microfluidic Device ◽  
High Throughput ◽  
Emulsification Process ◽  
Monodisperse Droplets

We present a comprehensive theoretical and experimental study of the microfluidic step-emulsification process for high-throughput production of monodisperse droplets.

scholarly journals Microfluidic Device for High-Throughput Production of Monodisperse Droplets

2020 ◽  
Vol 59 (28) ◽  
pp. 12784-12791
Author(s):  
Pierre Gelin ◽  
Ilyesse Bihi ◽  
Iwona Ziemecka ◽  
Benoit Thienpont ◽  
Jo Christiaens ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Monodisperse Droplets

A simple capillary-based open microfluidic device for size on-demand high-throughput droplet/bubble/microcapsule generation

Lab on a Chip ◽  
2018 ◽  
Vol 18 (18) ◽  
pp. 2806-2815 ◽  
Author(s):  
Liping Mei ◽  
Mingliang Jin ◽  
Shuting Xie ◽  
Zhibin Yan ◽  
Xin Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
On Demand ◽  
Monodisperse Droplets

A capillary-based open microfluidic device was established for flexible and controllable creation of monodisperse droplets of various fluidic materials.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Integrated impedance sensing of liquid sample plug flow enables automated high throughput NMR spectroscopy

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Omar Nassar ◽  
Mazin Jouda ◽  
Michael Rapp ◽  
Dario Mager ◽  
Jan G. Korvink ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Throughput ◽  
Plug Flow ◽  
Microfluidic System ◽  
High Resolution Spectroscopy ◽  
Dead Volume ◽  
Absolute Difference ◽  
Immiscible Fluid ◽  
Flow Sensors ◽  
Novel Approach

AbstractA novel approach for automated high throughput NMR spectroscopy with improved mass-sensitivity is accomplished by integrating microfluidic technologies and micro-NMR resonators. A flow system is utilized to transport a sample of interest from outside the NMR magnet through the NMR detector, circumventing the relatively vast dead volume in the supplying tube by loading a series of individual sample plugs separated by an immiscible fluid. This dual-phase flow demands a real-time robust sensing system to track the sample position and velocities and synchronize the NMR acquisition. In this contribution, we describe an NMR probe head that possesses a microfluidic system featuring: (i) a micro saddle coil for NMR spectroscopy and (ii) a pair of interdigitated capacitive sensors flanking the NMR detector for continuous position and velocity monitoring of the plugs with respect to the NMR detector. The system was successfully tested for automating flow-based measurement in a 500 MHz NMR system, enabling high resolution spectroscopy and NMR sensitivity of 2.18 nmol s1/2 with the flow sensors in operation. The flow sensors featured sensitivity to an absolute difference of 0.2 in relative permittivity, enabling distinction between most common solvents. It was demonstrated that a fully automated NMR measurement of nine individual 120 μL samples could be done within 3.6 min or effectively 15.3 s per sample.

scholarly journals Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

10.3791/52467 ◽  
2014 ◽  
Author(s):  
Derek S. Samarian ◽  
Nicholas S. Jakubovics ◽  
Ting L. Luo ◽  
Alexander H. Rickard
Keyword(s):  
High Throughput ◽  
Microfluidic System

Mass fabrication of uniform sized 3D tumor spheroid using high-throughput microfluidic system

2018 ◽  
Vol 275 ◽  
pp. 201-207 ◽  
Author(s):  
Bongseop Kwak ◽  
Yoohwan Lee ◽  
Jaehun Lee ◽  
Sungwon Lee ◽  
Jiseok Lim
Keyword(s):  
High Throughput ◽  
Microfluidic System ◽  
Tumor Spheroid

scholarly journals Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

2016 ◽  
Vol 113 (52) ◽  
pp. 14915-14920 ◽  
Author(s):  
Yih Yang Chen ◽  
Pamuditha N. Silva ◽  
Abdullah Muhammad Syed ◽  
Shrey Sindhwani ◽  
Jonathan V. Rocheleau ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Three Dimensional ◽  
Microfluidic System ◽  
High Throughput Drug Screening ◽  
Image Analysis Algorithm ◽  
Screening Assays ◽  
On Chip ◽  
Tissue Models

On-chip imaging of intact three-dimensional tissues within microfluidic devices is fundamentally hindered by intratissue optical scattering, which impedes their use as tissue models for high-throughput screening assays. Here, we engineered a microfluidic system that preserves and converts tissues into optically transparent structures in less than 1 d, which is 20× faster than current passive clearing approaches. Accelerated clearing was achieved because the microfluidic system enhanced the exchange of interstitial fluids by 567-fold, which increased the rate of removal of optically scattering lipid molecules from the cross-linked tissue. Our enhanced clearing process allowed us to fluorescently image and map the segregation and compartmentalization of different cells during the formation of tumor spheroids, and to track the degradation of vasculature over time within extracted murine pancreatic islets in static culture, which may have implications on the efficacy of beta-cell transplantation treatments for type 1 diabetes. We further developed an image analysis algorithm that automates the analysis of the vasculature connectivity, volume, and cellular spatial distribution of the intact tissue. Our technique allows whole tissue analysis in microfluidic systems, and has implications in the development of organ-on-a-chip systems, high-throughput drug screening devices, and in regenerative medicine.

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