scholarly journals An Open-Source Low-Cost Imaging System Plug-In for Pheromone Traps Aiding Remote Insect Pest Population Monitoring in Fruit Crops

Machines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 52
Author(s):  
Mark Jacob Schrader ◽  
Peter Smytheman ◽  
Elizabeth H. Beers ◽  
Lav R. Khot
Keyword(s):  
Imaging System ◽  
Low Cost ◽  
Insect Pest ◽  
Cydia Pomonella ◽  
Population Monitoring ◽  
Codling Moth ◽  
Sleep Mode ◽  
Pest Population ◽  
Image Capture ◽  
Commercial Applications

This note describes the development of a plug-in imaging system for pheromone delta traps used in pest population monitoring. The plug-in comprises an RGB imaging sensor integrated with a microcontroller unit and associated hardware for optimized power usage and data capture. The plug-in can be attached to the top of a modified delta trap to realize periodic image capture of the trap liner (17.8 cm × 17.8 cm). As configured, the captured images are stored on a microSD card with ~0.01 cm2 pixel−1 spatial resolution. The plug-in hardware is configured to conserve power, as it enters in sleep mode during idle operation. Twenty traps with plug-in units were constructed and evaluated in the 2020 field season for codling moth (Cydia pomonella) population monitoring in a research study. The units reliably captured images at daily interval over the course of two weeks with a 350 mAh DC power source. The captured images provided the temporal population dynamics of codling moths, which would otherwise be achieved through daily manual trap monitoring. The system’s build cost is about $33 per unit, and it has potential for scaling to commercial applications through Internet of Things-enabled technologies integration.

scholarly journals Monthly Weather Forecasts in a Pest Forecasting Context: Downscaling, Recalibration, and Skill Improvement

2012 ◽  
Vol 51 (9) ◽  
pp. 1633-1638 ◽  
Author(s):  
Martin Hirschi ◽  
Christoph Spirig ◽  
Andreas P. Weigel ◽  
Pierluigi Calanca ◽  
Jörg Samietz ◽  
...  
Keyword(s):  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Lead Times ◽  
Practical Applications ◽  
Weather Forecasts ◽  
Weather Information ◽  
Agricultural Applications ◽  
Resampling Procedure ◽  
Skill Improvement

AbstractMonthly weather forecasts (MOFCs) were shown to have skill in extratropical continental regions for lead times up to 3 weeks, in particular for temperature and if weekly averaged. This skill could be exploited in practical applications for implementations exhibiting some degree of memory or inertia toward meteorological drivers, potentially even for longer lead times. Many agricultural applications fall into these categories because of the temperature-dependent development of biological organisms, allowing simulations that are based on temperature sums. Most such agricultural models require local weather information at daily or even hourly temporal resolution, however, preventing direct use of the spatially and temporally aggregated information of MOFCs, which may furthermore be subject to significant biases. By the example of forecasting the timing of life-phase occurrences of the codling moth (Cydia pomonella), which is a major insect pest in apple orchards worldwide, the authors investigate the application of downscaled weekly temperature anomalies of MOFCs for use in an impact model requiring hourly input. The downscaling and postprocessing included the use of a daily weather generator and a resampling procedure for creating hourly weather series and the application of a recalibration technique to correct for the original underconfidence of the forecast occurrences of codling moth life phases. Results show a clear skill improvement of up to 3 days in root-mean-square error over the full forecast range when incorporating MOFCs as compared with deterministic benchmark forecasts using climatological information for predicting the timing of codling moth life phases.

scholarly journals Democratising “Microscopi”: a 3D printed automated XYZT fluorescence imaging system for teaching, outreach and fieldwork

2020 ◽  
Author(s):  
Matthew Wincott ◽  
Andrew Jefferson ◽  
Ian M. Dobbie ◽  
Martin J. Booth ◽  
Ilan Davis ◽  
...  
Keyword(s):  
Open Source ◽  
Fluorescence Imaging ◽  
Imaging System ◽  
Low Cost ◽  
Dark Field ◽  
List Type ◽  
Image Capture ◽  
Fluorescence Imaging System ◽  
3D Printed ◽  
Open Source Hardware

ABSTRACTCommercial fluorescence microscope stands and fully automated XYZt fluorescence imaging systems are generally beyond the limited budgets available for teaching and outreach. We have addressed this problem by developing “Microscopi”, an accessible, affordable, DIY automated imaging system that is built from 3D printed and commodity off-the-shelf hardware, including electro-mechanical, computer and optical components. Our design features automated sample navigation and image capture with a simple web-based graphical user interface, accessible with a tablet or other mobile device. The light path can easily be switched between different imaging modalities. The open source Python-based control software allows the hardware to be driven as an integrated imaging system. Furthermore, the microscope is fully customisable, which also enhances its value as a learning tool. Here, we describe the basic design and demonstrate imaging performance for a range of easily sourced specimens.HighlightsPortable, low cost, self-build from 3D printed and commodity componentsMultimodal imaging: bright field, dark field, pseudo-phase and fluorescenceAutomated XYZt imaging from a tablet or smartphone via a simple GUIWide ranging applications in teaching, outreach and fieldworkOpen source hardware and software design, allowing user modification

scholarly journals Economic Impact of a Potential Expansion of Pest Infestation: Apple Maggot in Washington State

2018 ◽  
Vol 28 (5) ◽  
pp. 651-659
Author(s):  
Suzette P. Galinato ◽  
R. Karina Gallardo ◽  
David M. Granatstein ◽  
Mike Willett
Keyword(s):  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Commercial Production ◽  
Washington State ◽  
Rhagoletis Pomonella ◽  
Pest Infestation ◽  
Additional Costs ◽  
Apple Production ◽  
Production Areas

Apple maggot (Rhagoletis pomonella) is an insect pest of apple (Malus domestica) that is currently limited in extent in the commercial production areas of Washington State thanks to a quarantine program. We estimate the costs to the Washington economy if this pest were to spread more widely. Apple maggot control costs are related to the pressure of codling moth (Cydia pomonella), the most prevalent insect pest in commercial apple production in Washington State. It was found that the losses for the Washington apple industry’s range from $510 million to $557 million, depending on the codling moth pressure. Our findings underscore the importance of an efficient quarantine program that minimized the risk of spreading the pest along with additional costs associated with quarantined areas.

Comparing the genetic structure of codling moth Cydia pomonella (L.) from Greece and France: long distance gene-flow in a sedentary pest species

2011 ◽  
Vol 102 (2) ◽  
pp. 185-198 ◽  
Author(s):  
C.Ch. Voudouris ◽  
P. Franck ◽  
J. Olivares ◽  
B. Sauphanor ◽  
Z. Mamuris ◽  
...  
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Differentiation ◽  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Pest Species ◽  
Long Distance ◽  
Control Programs ◽  
Genetic Clusters

AbstractCodling moth Cydia pomonella L. (Lepidoptera: Tortricidae) is the most important insect pest of apple production in Europe. Despite the economic importance of this pest, there is not information about the genetic structure of its population in Greece and the patterns of gene-flow which might affect the success of control programs. In this study, we analysed nine samples from apple, pear and walnut from various regions of mainland Greece using 11 microsatellite loci. Six samples from the aforementioned hosts from southern France were also examined for comparison. Bayesian clustering and genetic distance analyses separated the codling moth samples in two genetic clusters. The first cluster consisted mainly of the individuals from Greece, and the second of those from France, although admixture and miss-classified individuals were also observed. The low genetic differentiation among samples within each country was also revealed by FST statistics (0.009 among Greek samples and 0.0150 among French samples compared to 0.050 global value among all samples and 0.032 the mean of the pair-wise values between the two countries). These FST values suggest little structuring at large geographical scales in agreement with previous published studies. The host species and local factors (climatic conditions, topography, pest control programs) did not affect the genetic structure of codling moth populations within each country. The results are discussed in relation to human-made activities that promote gene-flow even at large geographic distances. Possible factors for the genetic differentiation between the two genetic clusters are also discussed.

scholarly journals Pest Management Challenges and Control Practices in Codling Moth: A Review

Insects ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Martina Kadoić Balaško ◽  
Renata Bažok ◽  
Katarina M. Mikac ◽  
Darija Lemic ◽  
Ivana Pajač Živković
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Cydia Pomonella ◽  
Genetic Research ◽  
Codling Moth ◽  
Fruit Production ◽  
Control Measures ◽  
Effective Control ◽  
Pome Fruit ◽  
Proactive Management

The codling moth, Cydia pomonella L., is a serious insect pest in pome fruit production worldwide with a preference for apple. The pest is known for having developed resistance to several chemical groups of insecticides, making its control difficult. The control and management of the codling moth is often hindered by a lack of understanding about its biology and ecology, including aspects of its population genetics. This review summarizes the information about the origin and biology of the codling moth, describes the mechanisms of resistance in this pest, and provides an overview of current research of resistant pest populations and genetic research both in Europe and globally. The main focus of this review is on non-pesticide control measures and anti-resistance strategies which help to reduce the number of chemical pesticides used and their residues on food and the local environment. Regular monitoring for insecticide resistance is essential for proactive management to mitigate potential insecticide resistance. Here we describe techniques for the detection of resistant variants and possibilities for monitoring resistance populations. Also, we present our present work on developing new methods to maintain effective control using appropriate integrated resistance management (IRM) strategies for this economically important perennial pest.

scholarly journals Bagging Apple Fruit for Codling Moth Control in Western Montana

2021 ◽  
pp. 1-4
Author(s):  
Rachel Leisso ◽  
Bridgid Jarrett ◽  
Katrina Mendrey ◽  
Zachariah Miller
Keyword(s):  
Insect Pest ◽  
Cydia Pomonella ◽  
Titratable Acidity ◽  
Codling Moth ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Small Scale ◽  
Forficula Auricularia ◽  
Plastic Bags ◽  
Second Year

Codling moth (Cydia pomonella) is a major insect pest of apple (Malus domestica). If unmanaged, then codling moth can infest nearly all apples in an orchard, where the flesh-tunneling larva leave frass-laden tracks in the fruit. Insecticide-based management requires accurate application timing (typically based on adult moth and/or degree-day monitoring) and multiple spray applications. Both the season-long commitment to codling moth monitoring and management and limited familiarity with insecticides, application tools, and proper application procedures can prevent a small-scale or backyard grower from effectively limiting fruit damage. In addition, an increasing segment of growers is interested in nonchemical alternatives. Bagging fruitlets early in the season could be a simple and effective method of codling moth management for this subset of growers. At our research orchard in Corvallis, MT, we tested a method combining fruit thinning and bagging using plastic bags the first season and nylon bags the second season. Plastic bags reduced the incidence of codling moth damage to fruit from 34% to 10%, but european earwig (Forficula auricularia) frass, which was found in more than 50% of plastic-bagged apples, made harvesting the fruit unappealing. We tested nylon fruit bags during the second year of the study. These bags did not significantly reduce the incidence of codling moth. Both the soluble solids content and titratable acidity were higher in unbagged fruit during the second year of the study, whereas color measurements indicated bagged fruit were greener on the shaded side of the fruit. Failure of the nylon bags may have been attributable to eggs laid before bagging, eggs laid or larva burrowing through bagging, or improper bag application methods. Further research could assess whole-tree bags, the addition of rubber bands or twist ties when applying nylon bags, pretreatment of fruit with horticulture oil, and/or dipping nylon bags in kaolin clay before application; however, these steps add time and increase costs, which may discourage the small-scale fruit grower. Overall, results indicate that fruit bagging holds promise for codling moth management; however, further work is needed to optimize the methodology.

scholarly journals Genome-Wide Selective Signature Analysis Revealed Insecticide Resistance Mechanisms in Cydia pomonella

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 2
Author(s):  
Wen-Ting Dai ◽  
Jin Li ◽  
Li-Ping Ban
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Glycine Receptor ◽  
Cydia Pomonella ◽  
Resistance Management ◽  
Codling Moth ◽  
Resistance Mechanisms ◽  
Expression Level ◽  
Invasive Pest ◽  
Cross Resistance

The codling moth, Cydia pomonella L. (Lepidoptera, Tortricidae), is a serious invasive pest of pome fruits. Currently, C. pomonella management mainly relies on the application of insecticides, which have driven the development of resistance in the insect. Understanding the genetic mechanisms of insecticide resistance is of great significance for developing new pest resistance management techniques and formulating effective resistance management strategies. Using existing genome resequencing data, we performed selective sweep analysis by comparing two resistant strains and one susceptible strain of the insect pest and identified seven genes, among which, two (glycine receptor and glutamate receptor) were under strong insecticide selection, suggesting their functional importance in insecticide resistance. We also found that eight genes including CYP6B2, CYP307a1, 5-hydroxytryptamine receptor, cuticle protein, and acetylcholinesterase, are potentially involved in cross-resistance to azinphos-methyl and deltamethrin. Moreover, among several P450s identified as positively selected genes, CYP6B2, CYP4C1, and CYP4d2 showed the highest expression level in larva compared to other stages tested, and CYP6B2 also showed the highest expression level in midgut, supporting the roles they may play in insecticide metabolism. Our results provide several potential genes that can be studied further to advance understanding of complexity of insecticide resistance mechanisms in C. pomonella.

scholarly journals 178 RESISTANCE TO CODLING MOTH: EXPRESSION OF SYNTHETIC CRYIA(C) GENES IN TRANSGENIC WALNUT EMBRYOS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 454d-454
Author(s):  
Abhava M. Dandekar ◽  
Gale H McGranahan ◽  
Sandra L. Uratsu ◽  
Charles Leslie ◽  
J. Steven Tebbets ◽  
...  
Keyword(s):  
Larval Development ◽  
Insect Pest ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
In Planta ◽  
Protein Fragments ◽  
Transgenic Embryo ◽  
Class B ◽  
Usage Patterns ◽  
Low Levels

Insecticidal crystal protein fragments (ICPFs) of Bacillus thuringiensis (Bt) encoded by cryIA(c) gene were shown in diet incorporation studies to be lethal to codling moth (CM; Cydia pomonella) the key insect pest for walnut. However transformed walnut tissues expressing cryIA(c) with Bt codon usage patterns and native DNA sequence revealed very low levels of expression in planta. To correct this problem synthetic versions of one of these genes, cryIA(c) was used to transform walnut tissue. A total of 61 individual transgenic embryo lines were obtained. 34% of these lines (21/61) were high expressors (“class A”) demonstrating 80 to 100% mortality of first in star CM larvae and displaying no further larval development. Twelve clones (20%) were designated “class B” and these showed a marked retardation of larval development and a mortality between 40 to 79%. Embryos from the remaining 28 lines designated “class C” (46%). although transformed, were indistinguishable from the control (untransformed embryos) and showed a mortality of 0 to 39%.

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