Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

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Self-Healing Bilayer Lipid Membranes Formed Over Synthetic Substrates

Smart Materials, Adaptive Structures and Intelligent Systems, Volume 2 ◽

10.1115/smasis2008-460 ◽

2008 ◽

Author(s):

M. Austin Creasy ◽

Donald J. Leo

Keyword(s):

Self Assembly ◽

Lipid Membranes ◽

Lipid Membrane ◽

Bilayer Lipid Membrane ◽

Unique Property ◽

Bilayer Lipid Membranes ◽

Self Healing ◽

Electrical Field ◽

Bilayer Lipid ◽

Recent Experimental Study

Biological systems demonstrate autonomous healing of damage and are an inspiration for developing self-healing materials. Our recent experimental study has demonstrated that a bilayer lipid membrane (BLM), also called a black lipid membrane, has the ability to self-heal after mechanical failure. These molecules have a unique property that they spontaneously self assembly into organized structures in an aqueous medium. The BLM forms an impervious barrier to ions and fluid between two volumes and strength of the barrier is dependent on the pressure and electrical field applied to the membrane. A BLM formed over an aperture on a silicon substrate is shown to self-heal for 5 pressurization failure cycles.

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Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

RSC Advances ◽

10.1039/c5ra09683a ◽

2015 ◽

Vol 5 (81) ◽

pp. 65684-65689 ◽

Cited By ~ 3

Author(s):

Fan Xu ◽

Meimei Bao ◽

Longfei Rui ◽

Jiaojiao Liu ◽

Jingliang Li ◽

...

Keyword(s):

Bombyx Mori ◽

Silk Fibroin ◽

Self Assembly ◽

Lipid Membranes ◽

Surface Coating ◽

Lipid Membrane ◽

Coating Film ◽

Silk Fibroin Film ◽

Bombyx Mori Silk ◽

Self Assembled

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.

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Assembling responsive microgels at responsive lipid membranes

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1807790116 ◽

2019 ◽

Vol 116 (12) ◽

pp. 5442-5450 ◽

Cited By ~ 11

Author(s):

Meina Wang ◽

Adriana M. Mihut ◽

Ellen Rieloff ◽

Aleksandra P. Dabkowska ◽

Linda K. Månsson ◽

...

Keyword(s):

Self Assembly ◽

Lipid Membranes ◽

Lipid Membrane ◽

Soft Materials ◽

Fluid Interfaces ◽

Biomedical Sciences ◽

Interfacial Assembly ◽

Colloidal Assembly ◽

Long Range Ordering ◽

Adsorption Desorption

Directed colloidal self-assembly at fluid interfaces can have a large impact in the fields of nanotechnology, materials, and biomedical sciences. The ability to control interfacial self-assembly relies on the fine interplay between bulk and surface interactions. Here, we investigate the interfacial assembly of thermoresponsive microgels and lipogels at the surface of giant unilamellar vesicles (GUVs) consisting of phospholipids bilayers with different compositions. By altering the properties of the lipid membrane and the microgel particles, it is possible to control the adsorption/desorption processes as well as the organization and dynamics of the colloids at the vesicle surface. No translocation of the microgels and lipogels through the membrane was observed for any of the membrane compositions and temperatures investigated. The lipid membranes with fluid chains provide highly dynamic interfaces that can host and mediate long-range ordering into 2D hexagonal crystals. This is in clear contrast to the conditions when the membranes are composed of lipids with solid chains, where there is no crystalline arrangement, and most of the particles desorb from the membrane. Likewise, we show that in segregated membranes, the soft microgel colloids form closely packed 2D crystals on the fluid bilayer domains, while hardly any particles adhere to the more solid bilayer domains. These findings thus present an approach for selective and controlled colloidal assembly at lipid membranes, opening routes toward the development of tunable soft materials.

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Kinetics of self-assembly of inclusions due to lipid membrane thickness interactions

Soft Matter ◽

10.1039/d0sm01752c ◽

2021 ◽

Author(s):

Xinyu Liao ◽

Prashant K. Purohit

Keyword(s):

Self Assembly ◽

Lipid Membranes ◽

Cell Biology ◽

Lipid Membrane ◽

Membrane Thickness ◽

Kinetics Of

Self-assembly of proteins on lipid membranes underlies many important processes in cell biology, such as, exo- and endo-cytosis, assembly of viruses, etc.

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One-Step Synthesis of a Fluorescent Phospholipid−Hydrogel Conjugate for Driving Self-Assembly of Supported Lipid Membranes

Macromolecules ◽

10.1021/ma0100899 ◽

2001 ◽

Vol 34 (17) ◽

pp. 5759-5765 ◽

Cited By ~ 19

Author(s):

Charlene C. Ng ◽

Yu-Ling Cheng ◽

Peter S. Pennefather

Keyword(s):

Self Assembly ◽

Lipid Membranes ◽

One Step ◽

Supported Lipid Membranes

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Characterization of Pore Formation by Streptolysin O on Supported Lipid Membranes by Impedance Spectroscopy and Surface Plasmon Resonance Spectroscopy

Langmuir ◽

10.1021/la0625502 ◽

2007 ◽

Vol 23 (3) ◽

pp. 1403-1409 ◽

Cited By ~ 22

Author(s):

Thomas Wilkop ◽

Danke Xu ◽

Quan Cheng

Keyword(s):

Surface Plasmon Resonance ◽

Impedance Spectroscopy ◽

Surface Plasmon ◽

Lipid Membranes ◽

Pore Formation ◽

Resonance Spectroscopy ◽

Surface Plasmon Resonance Spectroscopy ◽

Streptolysin O ◽

Supported Lipid Membranes

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Kinetics of self-assembly of inclusions due to lipid membrane thickness interactions

10.1101/2020.09.23.309575 ◽

2020 ◽

Author(s):

Xinyu Liao ◽

Prashant K. Purohit

Keyword(s):

Self Assembly ◽

Time Scale ◽

Lipid Membranes ◽

Lipid Membrane ◽

First Passage Time ◽

Langevin Dynamics ◽

Passage Time ◽

Membrane Thickness ◽

Kinetics Of ◽

Two Inclusions

AbstractSelf-assembly of proteins on lipid membranes underlies many important processes in cell biology, such as, exo- and endo-cytosis, assembly of viruses, etc. An attractive force that can cause self-assembly is mediated by membrane thickness interactions between proteins. The free energy profile associated with this attractive force is a result of the overlap of thickness deformation fields around the proteins. The thickness deformation field around proteins of various shapes can be calculated from the solution of a boundary value problem and is relatively well understood. Yet, the time scales over which self-assembly occurs has not been explored. In this paper we compute this time scale as a function of the initial distance between two inclusions by viewing their coalescence as a first passage time problem. The first passage time is computed using both Langevin dynamics and a partial differential equation, and both methods are found to be in excellent agreement. Inclusions of three different shapes are studied and it is found that for two inclusions separated by about hundred nanometers the time to coalescence is hundreds of milliseconds irrespective of shape. Our Langevin dynamics simulation of self-assembly required an efficient computation of the interaction energy of inclusions which was accomplished using a finite difference technique. The interaction energy profiles obtained using this numerical technique were in excellent agreement with those from a previously proposed semi-analytical method based on Fourier-Bessel series. The computational strategies described in this paper could potentially lead to efficient methods to explore the kinetics of self-assembly of proteins on lipid membranes.Author summarySelf-assembly of proteins on lipid membranes occurs during exo- and endo-cytosis and also when viruses exit an infected cell. The forces mediating self-assembly of inclusions on membranes have therefore been of long standing interest. However, the kinetics of self-assembly has received much less attention. As a first step in discerning the kinetics, we examine the time to coalescence of two inclusions on a membrane as a function of the distance separating them. We use both Langevin dynamics simulations and a partial differential equation to compute this time scale. We predict that the time to coalescence is on the scale of hundreds of milliseconds for two inclusions separated by about hundred nanometers. The deformation moduli of the lipid membrane and the membrane tension can affect this time scale.

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