Effects of Allelochemicals, Soil Enzyme Activities, and Environmental Factors on Rhizosphere Soil Microbial Community of Stellera chamaejasme L. along a Growth-Coverage Gradient

Allelochemicals released from the root of Stellera chamaejasme L. into rhizosphere soil are an important factor for its invasion of natural grasslands. The aim of this study is to explore the interactions among allelochemicals, soil physicochemical properties, soil enzyme activities, and the rhizosphere soil microbial communities of S. chamaejasme along a growth-coverage gradient. High-throughput sequencing was used to determine the microbial composition of the rhizosphere soil sample, and high-performance liquid chromatography was used to detect allelopathic substances. The main fungal phyla in the rhizosphere soil with a growth coverage of 0% was Basidiomycetes, and the other sample plots were Ascomycetes. Proteobacteria and Acidobacteria were the dominant bacterial phyla in all sites. RDA analysis showed that neochamaejasmin B, chamaechromone, and dihydrodaphnetin B were positively correlated with Ascomycota and Glomeromycota and negatively correlated with Basidiomycota. Neochamaejasmin B and chamaechromone were positively correlated with Proteobacteria and Actinobacteria and negatively correlated with Acidobacteria and Planctomycetes. Allelochemicals, soil physicochemical properties, and enzyme activity affected the composition and diversity of the rhizosphere soil microbial community to some extent. When the growth coverage of S. chamaejasme reached the primary stage, it had the greatest impact on soil physicochemical properties and enzyme activities.

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Change of soil microbial community under long-term fertilization in a reclaimed sandy agricultural ecosystem

PeerJ ◽

10.7717/peerj.6497 ◽

2019 ◽

Vol 7 ◽

pp. e6497 ◽

Cited By ~ 9

Author(s):

Zengru Wang ◽

Yubing Liu ◽

Lina Zhao ◽

Wenli Zhang ◽

Lichao Liu

Keyword(s):

Microbial Community ◽

Crop Yield ◽

Soil Microbial Community ◽

Enzyme Activities ◽

Bacterial Abundance ◽

Soil Enzyme ◽

Soil Enzyme Activities ◽

Soil Microbial Community Structure ◽

Soil Microbial

The importance of soil microbial flora in agro-ecosystems is well known, but there is limited understanding of the effects of long-term fertilization on soil microbial community succession in different farming management practices. Here, we report the responses of soil microbial community structure, abundance and activity to chemical (CF) and organic fertilization (OF) treatments in a sandy agricultural system of wheat-maize rotation over a 17-year period. Illumina MiSeq sequencing showed that the microbial community diversity and richness showed no significant changes in bacteria but decreased in fungi under both CF and OF treatments. The dominant species showing significant differences between fertilization regimes were Actinobacteria, Acidobacteria and Ascomycota at the phylum level, as well as some unclassified genera of other phyla at the genus level. As expected, soil organic matter content, nutrient element concentrations and bacterial abundance were enhanced by both types of fertilization, especially in OF, but fungal abundance was inhibited by OF. Redundancy analysis revealed that soil enzyme activities were closely related to both bacterial and fungal communities, and the soil nutrient, texture and pH value together determined the community structures. Bacterial abundance might be the primary driver of crop yield, and soil enzyme activities may reflect crop yield. Our results suggest a relatively permanent response of soil microbial communities to the long-term fertilization regimes in a reclaimed sandy agro-ecosystem from a mobile dune, and indicate that the appropriate dosage of chemical fertilizers is beneficial to sandy soil sustainability.

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Manure substitution of chemical fertilizer affect soil microbial community diversity, structure and function in greenhouse vegetable production systems

10.1101/570986 ◽

2019 ◽

Cited By ~ 1

Author(s):

Haoan Luan ◽

Wei Gao ◽

Shaowen Huang ◽

Jiwei Tang ◽

Mingyue Li ◽

...

Keyword(s):

Microbial Community ◽

Physicochemical Properties ◽

Soil Microbial Community ◽

Enzyme Activities ◽

Substitution Rate ◽

Soil Microbial Communities ◽

Chemical Fertilizer ◽

Soil Physicochemical Properties ◽

Soil Microbial ◽

Microbial Characteristics

ABSTRACTSoil microbial community and enzyme activities together affect various ecosystem functions of soils. Fertilization, as important agricultural management practices, are known to modify soil microbial characteristics; however, inconsistent results have been reported. The aim of this research therefore was to make a comparative study of the effects of different fertilization patterns (No N inputs (No N), 100% chemical fertilizer-N (CN) inputs (4/4CN) and different substitution rates of CN by organic manure-N (MN) (3/4CN+1/4MN, 2/4CN+2/4MN and 1/4CN+3/4MN)) on soil physicochemical properties, enzyme activities and microbial attributes in a GVP of Tianjin, China. Manure substitution of chemical fertilizer, especially at higher substitution rate (2/4CN+2/4MN and 1/4CN+3/4MN), improved soil physicochemical properties (higher soil organic C (SOC) and nutrient contents; lower bulk densities), promoted microbial growth (higher total phospholipid fatty acids and microbial biomass C contents) and activity (higher soil hydrolase activities). Manure addition caused a remarkable increase of the fungi/bacteria ratio and a distinct shift in the fungal (bacterial) community to greater abundance of arbuscular mycorrhizal fungi (G+ bacteria) compared with saprotrophic fungi (G− bacteria). These changes drove shifts toward fungal-dominated soil microbial communities and then optimized microbial community structure. Also, manure application increased soil biodiversity (microbial community and enzyme function), indicated by increased Shannon–Wiener diversity. Redundancy analysis indicated that the most possible mechanism of the impacts of different fertilization patterns on soil microbial characteristics may be the mediation of SOC and nutrient (N) availability (especially SOC) in this GVP of China. In conclusion, manure substitution of chemical fertilizer, especially at higher substitution rate, was more efficient for improving soil quality and biological functions.

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Effects of Winter Cover Crops Residue Returning on Soil Enzyme Activities and Soil Microbial Community in Double-Cropping Rice Fields

PLoS ONE ◽

10.1371/journal.pone.0100443 ◽

2014 ◽

Vol 9 (6) ◽

pp. e100443 ◽

Cited By ~ 15

Author(s):

Tang Hai-Ming ◽

Xiao Xiao-Ping ◽

Tang Wen-Guang ◽

Lin Ye-Chun ◽

Wang Ke ◽

...

Keyword(s):

Microbial Community ◽

Cover Crops ◽

Soil Microbial Community ◽

Enzyme Activities ◽

Soil Enzyme ◽

Soil Enzyme Activities ◽

Soil Microbial ◽

Double Cropping ◽

Winter Cover ◽

Winter Cover Crops

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Soil Microbial Community Analysis using Soil Enzyme Activities in Red Pepper Field Treated Microbial Agents

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2011.058 ◽

2012 ◽

Vol 55 (1) ◽

pp. 47-53 ◽

Cited By ~ 7

Author(s):

Yo-Hwan Kim ◽

Jong-Hui Lim ◽

Chang-Hwan An ◽

Byung-Kwon Jung ◽

Sang-Dal Kim

Keyword(s):

Microbial Community ◽

Soil Microbial Community ◽

Enzyme Activities ◽

Community Analysis ◽

Soil Enzyme ◽

Microbial Community Analysis ◽

Red Pepper ◽

Soil Enzyme Activities ◽

Soil Microbial ◽

Microbial Agents

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Responses of Soil Microbial Community and Enzyme Activities to Shrub Species Artemisia gmelinii in Relation to Varying Rainfall in a Semiarid Land, SW China

Frontiers in Environmental Science ◽

10.3389/fenvs.2021.725960 ◽

2021 ◽

Vol 9 ◽

Author(s):

Laiye Qu ◽

Bingbing Wang ◽

Xinyu Zhang ◽

Minggang Wang

Keyword(s):

Microbial Community ◽

Soil Microbial Community ◽

Enzyme Activities ◽

Climatic Variation ◽

Soil Physicochemical Properties ◽

Shrub Species ◽

Soil Microbial ◽

Canopy Soil ◽

Shrub Canopy ◽

Semiarid Land

Widely distributed shrubs in drylands can locally alter soil physicochemical properties, which distinguish soil under plant canopy from soil outside the canopy. In the present study, we used a dominant shrub species Artemisia gmelinii in a semiarid land, SW China, to investigate the consequences of “shrub resource islands” for soil microbial communities and enzymatic activities. Such investigation was made at four sites that differed in rates of rainfall to examine how the consequences were altered by variation in the local climate. The results showed that A. gmelinii enhanced fungal abundance but did not influence bacterial abundance, resulting in higher total microbial abundance and fungal-to-bacterial ratio in under-canopy soil compared to outside-canopy soil. Microbial community composition also differed between the two soils, but this difference only occurred at sites of low rainfall. Redundancy analysis revealed that such composition was attributed to variation in soil water content, bulk density, and total phosphorus as a result of shrub canopy and varying rates of rainfall. Activities of hydrolytic enzymes (β-1,4-glucosidase, β-1,4-N-acetylglucosaminidase, alkaline phosphatase, and leucine aminopeptidase) were higher in under-canopy soil than in outside-canopy soil, among which C-acquisition enzyme, β-1,4-glucosidase, and P-acquisition enzyme, alkaline phosphatase, were also higher in the soil of high rainfall. The overall pattern of enzyme activities did not show differences between under- and outside-canopy soils, but it separated the sites of high rate from that of low rates of rainfall. This pattern was primarily driven by variation in soil physicochemical properties rather than variation in soil microbial community, suggesting that the distribution pattern of enzyme activities may be more sensitive to variation in rainfall than to shrub canopy. In conclusion, our study shows that shrub species A. gmelinii can shift the soil microbial community to be fungal-dominant and increase hydrolytic enzyme activities, and such effect may depend on local climatic variation, for example, rainfall changes in the semiarid land. The findings of this study highlight the important roles of shrub vegetation in soil biological functions and the sensitivity of such roles to climatic variation in semiarid ecosystems.

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Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China

Sustainability ◽

10.3390/su132111684 ◽

2021 ◽

Vol 13 (21) ◽

pp. 11684

Author(s):

Li Fan ◽

Weiping Zhao ◽

Wendan Feng ◽

Ping Mo ◽

Yunlin Zhao ◽

...

Keyword(s):

Microbial Community ◽

Physicochemical Properties ◽

Microbial Diversity ◽

Ecological Restoration ◽

Soil Microbial Community ◽

Mining Area ◽

Soil Restoration ◽

Soil Physicochemical Properties ◽

Soil Microbial Diversity ◽

Soil Microbial

Soil microorganisms play an important role in regulating a variety of ecological functions. In recent years, the research on ecological restoration after mining has made people more aware of the importance of microbial diversity to ecosystem restoration. The present study investigated the effect of ecological restoration on microbial community structure and its relationship with soil physicochemical properties in the Dabaoshan mining area, China. High throughput sequencing technology was used to analyze and compare the microbial community composition of three types of soil (undamaged area, unrestoration area, and ecological restoration area). The contents of organic carbon, total nitrogen, and total phosphorus were 2.38–12.97 g/kg, 0.39–1.62 g/kg, and 0.99–1.51 g/kg, respectively. In different soil states, undamaged area and ecological restoration area were significantly higher than those in unrestoration area. The results showed that the structure of soil microbial community was significantly correlated with soil physicochemical properties, and formations in the repaired and unrepaired soils were different. Operational Taxonomic Unit (OTU) cluster analysis and diversity index analysis showed that soil microbial community changed at phylum and genus levels. The results showed that at the phylum level, all soil samples contained Firmicutes, Proteobacteria, and actinobacteria. Firmicutes and Proteobacteria of the ecological restoration area (ER1, ER2) were the highest in relative abundance compared with other samples, accounting for more than 45%. Proteobacteria and Acidobacteria were the dominant phylum in the undamaged area (UD), accounting for 32.7% and 22.3%, respectively. It can be seen that soil restoration produced a new dominant population, and Proteobacteria showed an absolute competitive advantage in the mining soil.

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