scholarly journals Analysis of Bacterial Communities on North Sea Macroalgae and Characterization of the Isolated Planctomycetes Adhaeretor mobilis gen. nov., sp. nov., Roseimaritima multifibrata sp. nov., Rosistilla ulvae sp. nov. and Rubripirellula lacrimiformis sp. nov.

2021 ◽  
Vol 9 (7) ◽  
pp. 1494
Author(s):  
Sandra Wiegand ◽  
Patrick Rast ◽  
Nicolai Kallscheuer ◽  
Mareike Jogler ◽  
Anja Heuer ◽  
...  

Planctomycetes are bacteria that were long thought to be unculturable, of low abundance, and therefore neglectable in the environment. This view changed in recent years, after it was shown that members of the phylum Planctomycetes can be abundant in many aquatic environments, e.g., in the epiphytic communities on macroalgae surfaces. Here, we analyzed three different macroalgae from the North Sea and show that Planctomycetes is the most abundant bacterial phylum on the alga Fucus sp., while it represents a minor fraction of the surface-associated bacterial community of Ulva sp. and Laminaria sp. Especially dominant within the phylum Planctomycetes were Blastopirellula sp., followed by Rhodopirellula sp., Rubripirellula sp., as well as other Pirellulaceae and Lacipirellulaceae, but also members of the OM190 lineage. Motivated by the observed abundance, we isolated four novel planctomycetal strains to expand the collection of species available as axenic cultures since access to different strains is a prerequisite to investigate the success of planctomycetes in marine environments. The isolated strains constitute four novel species belonging to one novel and three previously described genera in the order Pirellulales, class Planctomycetia, phylum Planctomycetes.

1992 ◽  
Vol 68 (1) ◽  
pp. 11-19 ◽  
Author(s):  
A. Oshaug ◽  
L. I. Østgård ◽  
K. U. Trygg

Dietary studies based on 24 h recalls were carried out on four oil installations in the Norwegian sector of the North Sea. Two hundred and three persons were interviewed about what they had eaten the previous 24 h. Food purchased for the installations in the previous 5 months was recorded. Results based on 24 h recalls showed that average daily intake of energy was 12.2 MJ of which 17% came from protein, 44% from fat and 39% from carbohydrate, including 8% from sugar. Meat, vegetables, fresh fruits, seafood (shellfish), french fries, eggs, cream and ice-cream were important components of the diet, while bread, fish and cereals played a minor role. Average daily intake (mg) of nutrients were: calcium 1244, iron 15, vitamin A 1049 μg, vitamin D 4.1 μg, thiamin 1.6, riboflavin 2.2, nicotinic acid 22, ascorbic acid 143. Dietary fibre intake, estimated as unavailable carbohydrate, was on average 19 g, and the average daily intake of cholesterol was 755 mg. Intakes were compared with the Norwegian recommended dietary allowance. Most of the employees chose a diet which when eaten over a longer period of time may contribute to the development of coronary heart diseases (CHD) and thereby increase the morbidity and mortality from CHD in the oil industry.


2011 ◽  
Vol 8 (9) ◽  
pp. 2547-2560 ◽  
Author(s):  
J. Logemann ◽  
J. Graue ◽  
J. Köster ◽  
B. Engelen ◽  
J. Rullkötter ◽  
...  

Abstract. Intact polar lipids (IPLs) are considered biomarkers for living biomass. Their degradation in marine sediments, however, is poorly understood and complicates interpretation of their occurrence in geological samples. To investigate the turnover of IPLs, a degradation experiment with anoxic sandy sediments from the North Sea was conducted. Intact cells of two organisms that do not naturally occur in North Sea sediments were chosen as IPL sources: (i) Saccharomyces cerevisiae, representative for ester-bound acyl lipids that also occur in Bacteria, and (ii) the archaeon Haloferax volcanii, representative for ether-bound isoprenoid lipids. Surprisingly, IPLs with phosphoester-bound head groups showed approximately the same degradation rate as IPLs with glycosidic head groups. Furthermore, the results indicate a relatively fast degradation of S. cerevisiae IPLs with ester-bound moieties (analogs of bacterial membrane lipids) and no significant degradation of archaeal IPLs with ether-bound moieties. Pore water and 16S rRNA-based DGGE analysis showed only a minor influence of the IPL source on microbial metabolism and community profiles. Due to our results, the IPL-based quantification of Archaea and Bacteria should be interpreted with caution.


2012 ◽  
Vol 12 (1) ◽  
pp. 203 ◽  
Author(s):  
Othilde Håvelsrud ◽  
Thomas HA Haverkamp ◽  
Tom Kristensen ◽  
Kjetill S Jakobsen ◽  
Anne Rike

2011 ◽  
Vol 8 (2) ◽  
pp. 3289-3321 ◽  
Author(s):  
J. Logemann ◽  
J. Graue ◽  
J. Köster ◽  
B. Engelen ◽  
J. Rullkötter ◽  
...  

Abstract. Intact polar lipids (IPLs) are considered biomarkers for living biomass. Their degradation in marine sediments, however, is poorly understood and complicates interpretation of their occurrence in geological samples. To investigate the turnover of IPLs, a degradation experiment with anoxic sandy sediments from the North Sea was conducted. Intact cells of two organisms that do not naturally occur in North Sea sediments were chosen as IPL sources: (i) Saccharomyces cerevisiae, representative for ester-bound acyl lipids that also occur in Bacteria, and (ii) the archaeon Haloferax volcanii, representative for ether-bound isoprenoid lipids. Surprisingly, IPLs with phosphoester-bound head groups showed approximately the same degradation rate as IPLs with glycosidic head groups. Furthermore, the results indicate a relatively fast degradation of S. cerevisiae IPLs with ester-bound moieties (analogs of bacterial membrane lipids) and no significant degradation of archaeal IPLs with ether-bound moieties. Pore water and 16S rRNA-based DGGE analysis showed only a minor influence of the IPL source on microbial metabolism and community profiles. Due to our results, the IPL-based quantification of Archaea and Bacteria should be interpreted with caution.


2014 ◽  
Vol 8 (3) ◽  
pp. 434-447 ◽  
Author(s):  
Panagiota-Myrsini Chronopoulou ◽  
Gbemisola O. Sanni ◽  
Daniel I. Silas-Olu ◽  
Jan Roelof van der Meer ◽  
Kenneth N. Timmis ◽  
...  

Geophysics ◽  
2002 ◽  
Vol 67 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Richard T. Houck

Lithologic interpretations of amplitude variation with offset (AVO) information are ambiguous both because different lithologies occupy overlapping ranges of elastic properties, and because angle‐dependent reflection coefficients estimated from seismic data are uncertain. This paper presents a method for quantifying and combining these two components of uncertainty to get a full characterization of the uncertainty associated with an AVO‐based lithologic interpretation. The result of this approach is a compilation of all the lithologies that are consistent with the observed AVO behavior, along with a probability of occurrence for each lithology. A 2‐D line from the North Sea illustrates how the method might be applied in practice. For any data set, the interaction between the geologic and measurement components of uncertainty may significantly affect the overall uncertainty in a lithologic interpretation.


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