scholarly journals Serum Metabolic Profiling Reveals Potential Anti-Inflammatory Effects of the Intake of Black Ginseng Extracts in Beagle Dogs

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3759 ◽  
Author(s):  
Ye Jin Kim ◽  
Dae Young Lee ◽  
Ho-Eun Park ◽  
Dahye Yoon ◽  
Bumkyu Lee ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Serum Biomarkers ◽  
Beagle Dogs ◽  
High Concentration ◽  
Experimental Diet ◽  
Inflammatory Processes ◽  
Black Ginseng ◽  
White Ginseng ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Black ginseng (BG) has better health benefits than white ginseng. The intake of BG changes the levels of metabolites, such as amino acids, fatty acids, and other metabolites. However, there is no research on the effect of BG extract intake on the metabolic profile of dog serum. In this study, serum metabolic profiling was conducted to investigate metabolic differences following the intake of BG extracts in beagle dogs. The beagle dogs were separated into three groups and fed either a regular diet (RD, control), RD with a medium concentration of BG extract (BG-M), or RD with a high concentration of BG extract (BG-H). Differences were observed among the three groups after the dogs ingested the experimental diet for eight weeks. The concentrations of alanine, leucine, isoleucine, and valine changed with the intake of BG extracts. Furthermore, levels of glycine and β-alanine increased in the BG-H group compared to the control and BG-M groups, indicating that BG extracts are associated with anti-inflammatory processes. Our study is the first to demonstrate the potential anti-inflammatory effect of BG extract in beagle dogs. Glycine and β-alanine are proposed as candidate serum biomarkers in dogs that can discriminate between the effects of ingesting BG-H.

Metabolic Profiling to Identify Potential Serum Biomarkers for Gastric Ulceration Induced by Nonsteroid Anti-Inflammatory Drugs

2013 ◽  
Vol 12 (3) ◽  
pp. 1399-1407 ◽  
Author(s):  
Kenichiro Takeuchi ◽  
Maki Ohishi ◽  
Sana Ota ◽  
Kenichi Suzumura ◽  
Hitoshi Naraoka ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Serum Biomarkers ◽  
Gastric Ulceration ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Berberine Inhibits the gene Expression and Production of Proinflammatory Cytokines by Mononuclear cells in Rheumatoid Arthritis and Healthy Individuals

2020 ◽  
Vol 16 ◽  
Author(s):  
Niloofar Ghorbani ◽  
Maryam Sahebari ◽  
Mahmoud Mahmoudi ◽  
Maryam Rastin ◽  
Shahrzad Zamani ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Mononuclear Cells ◽  
Inhibitory Effect ◽  
Healthy Individuals ◽  
High Concentration ◽  
Antiinflammatory Property ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Objective: Rheumatoid arthritis (RA) is the most prevalent autoimmune arthritis. Berberine is an alkaloid isolated from Berberis vulgaris and its anti-inflammatory effect has been identified. Method: Twenty newly diagnosed RA patients and 20 healthy controls participated. Peripheral mononuclear cells were prepared and stimulated with bacterial lipopolysachharide (LPS,1 µg/ml), exposed to different concentrations of berberine (10 and 50µM) and dexamethasone (10-7 M) as a reference. Toxicity of compounds was evaluated by WST-1 assay. Expression of TNF-α and IL-1β were determined by quantitative real-time PCR. Protein level of secreted TNF-α and IL1β were measured by using ELISA. Result: Berberine did not have any toxic effect on cells, whereas Lipopolysachharide (LPS) stimulation caused a noticeable rise in TNF-α and IL-1β production. Berberine markedly downregulated the expression of both TNF-α and IL1β and inhibits TNF-α and IL-1β secretion from LPS-stimulated PBMCs. Discussion: This study provided molecular basis for anti-inflammatory effect of berberine on human mononuclear cells through the suppression of TNF-a and IL-1secretion. Our findings highlighted the significant inhibitory effect of berberine on proinflammatory responses of mononuclear cells from rheumatoid arthritis individuals, which may be responsible for antiinflammatory property of Barberry. We observed that berberine at high concentration exhibited anti-inflammatory effect in PBMCs of both healthy and patient groups by suppression of TNF-a and IL-1cytokines at both mRNA and protein levels. Conclusions: Berberine may inhibit the gene expression and production of pro-inflammatory cytokines by mononuclear cells in rheumatoid arthritis and healthy individuals without affecting cells viability. Future studies with larger sample size is needed to prove the idea.

scholarly journals Inhibition of Neutrophil Secretion Upon Adhesion as a Basis for the Anti-Inflammatory Effect of the Tricyclic Antidepressant Imipramine

2021 ◽  
Vol 12 ◽  
Author(s):  
Svetlana I. Galkina ◽  
Ekaterina A. Golenkina ◽  
Natalia V. Fedorova ◽  
Alexander L. Ksenofontov ◽  
Marina V. Serebryakova ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Neutrophil Infiltration ◽  
Tricyclic Antidepressant ◽  
Basement Membranes ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Lysine Residues ◽  
Antidepressant Imipramine ◽  
Inflammatory Processes ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Recent studies demonstrate the involvement of inflammatory processes in the development of depression and the anti-inflammatory effects of antidepressants. Infiltration and adhesion of neutrophils to nerve tissues and their aggressive secretion are considered as possible causes of inflammatory processes in depression. We studied the effect of the antidepressant imipramine on the adhesion and accompanied secretion of neutrophils under control conditions and in the presence of lipopolysaccharides (LPS). As a model of integrin-dependent neutrophil infiltration into tissues, we used integrin-dependent adhesion of neutrophils to the fibronectin-coated substrate. Imipramine inhibited neutrophil adhesion and concomitant secretion of proteins, including matrix metalloproteinase 9 (MMP-9) and neutrophil gelatinase-associated lipocalin (NGAL), which modify the extracellular matrix and basement membranes required for cell migration. Imipramine also significantly and selectively blocked the release of the free amino acid hydroxylysine, a product of lysyl hydroxylase, an enzyme that affects the organization of the extracellular matrix by modifying collagen lysine residues. In contrast, imipramine enhanced the release of ROS by neutrophils during adhesion to fibronectin and stimulated apoptosis. The anti-inflammatory effect of imipramine may be associated with the suppression of neutrophil infiltration and their adhesion to nerve tissues by inhibiting the secretion of neutrophils, which provides these processes.

scholarly journals High Induction of IL-6 Secretion From hUCMSCs Optimize the Potential of hUCMSCs and TCZ as Therapy for COVID-19-Related ARDS

2021 ◽  
Vol 30 ◽  
pp. 096368972110544
Author(s):  
Huei-Yu Lo ◽  
Shun-Ping Cheng ◽  
Jing-Long Huang ◽  
Kuo-Ting Chang ◽  
Yu-Lung Chang ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Therapeutic Potential ◽  
Neutralizing Antibody ◽  
Inhibitory Effect ◽  
Human Umbilical Cord ◽  
High Concentration ◽  
Peripheral Blood Mononuclear ◽  
Potent Inhibitory Effect ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Biological and cellular interleukin-6 (IL-6)-related therapies have been used to treat severe COVID-19 pneumonia with hyperinflammatory syndrome and acute respiratory failure, which prompted further exploration of the role of IL-6 in human umbilical cord mesenchymal stem cell (hUCMSC) therapy. Peripheral blood mononuclear cells (PBMCs) were responders cocultured with hUCMSCs or exogenous IL-6. A PBMC suppression assay was used to analyze the anti-inflammatory effects via MTT assay. The IL-6 concentration in the supernatant was measured using ELISA. The correlation between the anti-inflammatory effect of hUCMSCs and IL-6 levels and the relevant roles of IL-6 and IL-6 mRNA expression was analyzed using the MetaCore functional network constructed from gene microarray data. The location of IL-6 and IL-6 receptor (IL-6R) expression was further evaluated. We reported that hUCMSCs did not initially exert any inhibitory effect on PHA-stimulated proliferation; however, a potent inhibitory effect on PHA-stimulated proliferation was observed, and the IL-6 concentration reached approximately 1000 ng/mL after 72 hours. Exogenous 1000 ng/mL IL-6 inhibited PHA-stimulated inflammation but less so than hUCMSCs. The inhibitory effects of hUCMSCs on PHA-stimulated PBMCs disappeared after adding an IL-6 neutralizing antibody or pretreatment with tocilizumab (TCZ), an IL-6R antagonist. hUCMSCs exert excellent anti-inflammatory effects by inducing higher IL-6 levels, which is different from TCZ. High concentration of IL-6 cytokine secretion plays an important role in the anti-inflammatory effect of hUCMSC therapy. Initial hUCMSC therapy, followed by TCZ, seems to optimize the therapeutic potential to treat COVID-19-related acute respiratory distress syndrome (ARDS).

scholarly journals Trefoil Factor Family Member 2 (TFF2) as an Inflammatory-Induced and Anti-Inflammatory Tissue Repair Factor

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1646
Author(s):  
Abdelaziz Ghanemi ◽  
Mayumi Yoshioka ◽  
Jonny St-Amand
Keyword(s):  
Family Member ◽  
Tissue Repair ◽  
Trefoil Factor ◽  
Mucosal Repair ◽  
Inflammatory Processes ◽  
Trefoil Factor Family ◽  
Repair Factor ◽  
Anti Inflammatory ◽  
Inflammatory Effect ◽  
Inflammatory Tissue

Trefoil factor family member 2 (TFF2) is known for its involvement in mucosal repair. Whereas it is overexpressed during inflammatory processes, adding TFF2 leads to an anti-inflammatory effect that would contribute to create the microenvironment required for tissue repair. These properties present TFF2 with a homeostatic pattern during inflammatory processes as illustrated by selected examples.

Influence of polymer-organic solvent ratio on the physical and technological characteristics of gimantan nanoparticles

2020 ◽  
pp. 25-28
Author(s):  
V. Markeev ◽  
E. Blynskaya ◽  
A. Marakhova ◽  
K. Alekseev
Keyword(s):  
Organic Solvent ◽  
Targeted Delivery ◽  
Polymer Nanoparticles ◽  
Solvent Ratio ◽  
Pharmaceutical Substance ◽  
Technological Characteristics ◽  
Inflammatory Processes ◽  
The Creation ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Ensuring targeted delivery of drugs is the most promising and studied direction in pharmacy. This task can be solved by creating nanostructures that can accumulate in the focus of inflammation in case of treatment of inflammatory processes. The results of studies on the creation of polymer nanoparticles with an encapsulated pharmaceutical substance of gimantan, which has a local anti-inflammatory effect, are described.

Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

2019 ◽  
pp. 1-9
Author(s):  
Samira Salem ◽  
Essaid Leghouchi ◽  
Rachid Soulimani ◽  
Jaouad Bouayed
Keyword(s):  
Time Course ◽  
Acute Inflammation ◽  
Lichen Species ◽  
Paw Edema ◽  
Sod Activity ◽  
Edema Formation ◽  
Lobaria Pulmonaria ◽  
Anti Inflammatory ◽  
Endogenous Antioxidant ◽  
Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

Anti-inflammatory effect of Curcuma longa (turmeric) rhizome when administered topically in gel form

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
AH Mollik ◽  
W Mozammel Haq ◽  
S Chandra Bachar ◽  
R Jahan ◽  
M Rahmatullah
Keyword(s):  
Curcuma Longa ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect

Anti-inflammatory effect of Uncaria tomentosa in an experimental model of cyclophosphamide-induced hemorrhagic cystitis

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
IST Figueiredo ◽  
FT Benevides ◽  
NMS Queiroz ◽  
LM Marques ◽  
TFG Souza ◽  
...  
Keyword(s):  
Experimental Model ◽  
Hemorrhagic Cystitis ◽  
Uncaria Tomentosa ◽  
Anti Inflammatory ◽  
Inflammatory Effect
Sign in / Sign up

Export Citation Format

Share Document