scholarly journals Treatment with Uncaria tomentosa Promotes Apoptosis in B16-BL6 Mouse Melanoma Cells and Inhibits the Growth of B16-BL6 Tumours

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1066
Author(s):  
Ali Zari ◽  
Hajer Alfarteesh ◽  
Carly Buckner ◽  
Robert Lafrenie
Keyword(s):  
Tumour Size ◽  
Melanoma Cells ◽  
Tunel Staining ◽  
Aqueous Extracts ◽  
Ki 67 ◽  
Uncaria Tomentosa ◽  
Mouse Melanoma ◽  
Anti Cancer

Uncaria tomentosa is a medicinal plant native to Peru that has been traditionally used in the treatment of various inflammatory disorders. In this study, the effectiveness of U. tomentosa as an anti-cancer agent was assessed using the growth and survival of B16-BL6 mouse melanoma cells. B16-BL6 cell cultures treated with both ethanol and phosphate-buffered saline (PBS) extracts of U. tomentosa displayed up to 80% lower levels of growth and increased apoptosis compared to vehicle controls. Treatment with ethanolic extracts of Uncaria tomentosa were much more effective than treatment with aqueous extracts. U. tomentosa was also shown to inhibit B16-BL6 cell growth in C57/bl mice in vivo. Mice injected with both the ethanolic and aqueous extracts of U. tomentosa showed a 59 ± 13% decrease in B16-BL6 tumour weight and a 40 ± 9% decrease in tumour size. Histochemical analysis of the B16-BL6 tumours showed a strong reduction in the Ki-67 cell proliferation marker in U. tomentosa-treated mice and a small, but insignificant increase in terminal transferase dUTP nick labelling (TUNEL) staining. Furthermore, U. tomentosa extracts reduced angiogenic markers and reduced the infiltration of T cells into the tumours. Collectively, the results in this study concluded that U. tomentosa has potent anti-cancer activity that significantly inhibited cancer cells in vitro and in vivo.

scholarly journals Molecular Mechanisms of Anti-Melanogenic Gedunin Derived from Neem Tree (Azadirachta indica) Using B16F10 Mouse Melanoma Cells and Early-Stage Zebrafish

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 330
Author(s):  
Hwang-Ju Jeon ◽  
Kyeongnam Kim ◽  
Chaeeun Kim ◽  
Myoung-Jin Kim ◽  
Tae-Oh Kim ◽  
...  
Keyword(s):  
Skin Color ◽  
Molecular Mechanisms ◽  
Early Stage ◽  
Melanoma Cells ◽  
Ultraviolet Rays ◽  
Melanin Production ◽  
Zebrafish Model ◽  
Mouse Melanoma

Melanogenesis represents a series of processes that produce melanin, a protective skin pigment (against ultraviolet rays), and determines human skin color. Chemicals reducing melanin production have always been in demand in the cosmetic market because of skincare interests, such as whitening. The main mechanism for inhibiting melanin production is the inhibition of tyrosinase (TYR), a key enzyme for melanogenesis. Here, we evaluated gedunin (Ged), a representative limonoid, for its anti-melanogenesis action. Melanin production in vitro was stimulated by alpha-melanocyte stimulating hormone (α-MSH) in B16F10 mouse melanoma cells. Ged reduced α-MSH-stimulated melanin production, inhibiting TYR activity and protein amount. We confirmed this result in vivo in a zebrafish model for melanogenesis. There was no sign of toxicity and malformation of zebrafish embryos during development in all treated concentrations. Ged reduced the number of produced zebrafish embryo pigment dots and melanin contents of embryos. The highly active concentration of Ged (100 µM) was much lower than the positive control, kojic acid (8 mM). Hence, Ged could be a fascinating candidate for anti-melanogenesis reagents.

Solanum nigrum Linn. Water Extract Inhibits Metastasis in Mouse Melanoma Cells in Vitro and in Vivo

2010 ◽  
Vol 58 (22) ◽  
pp. 11913-11923 ◽  
Author(s):  
Hsueh-Chun Wang ◽  
Dun-Hao Wu ◽  
Yun-Ching Chang ◽  
Yi-Ju Li ◽  
Chau-Jong Wang
Keyword(s):  
Water Extract ◽  
Melanoma Cells ◽  
Solanum Nigrum ◽  
Mouse Melanoma

scholarly journals Rho Kinase Inhibitor Fasudil Suppresses the Vasculogenic Mimicry of B16 Mouse Melanoma Cells Both In Vitro and In Vivo

2015 ◽  
Vol 14 (7) ◽  
pp. 1582-1590 ◽  
Author(s):  
Yun Xia ◽  
Xian-Yi Cai ◽  
Ji-Quan Fan ◽  
Li-Ling Zhang ◽  
Jing-Hua Ren ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Vasculogenic Mimicry ◽  
Rho Kinase ◽  
Melanoma Cells ◽  
Mouse Melanoma ◽  
Rho Kinase Inhibitor ◽  
B16 Mouse Melanoma

scholarly journals Cisplatin-Induced Giant Cells Formation Is Involved in Chemoresistance of Melanoma Cells

2020 ◽  
Vol 21 (21) ◽  
pp. 7892
Author(s):  
Chien-Hui Weng ◽  
Chieh-Shan Wu ◽  
Jian-Ching Wu ◽  
Mei-Lang Kung ◽  
Ming-Hsiu Wu ◽  
...  
Keyword(s):  
Multiple Drug Resistance ◽  
Giant Cells ◽  
Melanoma Cells ◽  
Cell Diameter ◽  
Multiple Drug ◽  
Stem Cell Markers ◽  
Ki 67 ◽  
Cancer Stem Cell Markers

Melanoma is notoriously resistant to current cancer therapy. However, the chemoresistance mechanism of melanoma remains unclear. The present study unveiled that chemotherapy drug cisplatin induced the formation of giant cells, which exhibited enlargement in cell diameter and nucleus in mice and human melanoma cells. Giant cells were positive with melanoma maker S100 and cancer stem cell markers including ABCB5 and CD133 in vitro and in vivo. Moreover, giant cells retained the mitotic ability with expression of proliferation marker Ki-67 and exhibited multiple drug resistance to doxorubicin and actinomycin D. The mitochondria genesis/activities and cellular ATP level were significantly elevated in giant cells, implicating the demand for energy supply. Application of metabolic blockers such as sodium azide or 2-deoxy glucose abolished the cisplatin-induced giant cells formation and expression of cancer stemness markers. The present study unveils a novel chemoresistance mechanism of melanoma cells via size alteration and the anti-neoplastic strategy by targeting giant cells.

scholarly journals PRL-3 siRNA Inhibits the Metastasis of B16-BL6 Mouse Melanoma Cells In Vitro and In Vivo

2007 ◽  
Vol 13 (3-4) ◽  
pp. 151-159 ◽  
Author(s):  
Feng Qian ◽  
Yu-Pei Li ◽  
Xia Sheng ◽  
Zi-Chao Zhang ◽  
Ran Song ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Mouse Melanoma

scholarly journals Erratum to: PRL-3 siRNA Inhibits the Metastasis of B16-BL6 Mouse Melanoma Cells In Vitro and In Vivo

2007 ◽  
Vol 13 (5-6) ◽  
pp. 325-325
Author(s):  
Feng Qian ◽  
Yu-Pei Li ◽  
Xia Sheng ◽  
Zi-Chao Zhang ◽  
Ran Song ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Mouse Melanoma

Nanoencapsulation of Andrographolide Rich Extract for the Inhibition of Cervical and Neuroblastoma Cancer Cells

2020 ◽  
Vol 16 (9) ◽  
pp. 1370-1380
Author(s):  
Parisa Sanati ◽  
Lee Suan Chua ◽  
Rozita Nasiri ◽  
Seyedeh-Sara Hashemi
Keyword(s):  
Tumour Size ◽  
Neuroblastoma Cells ◽  
Ki 67 ◽  
Normal Human Skin ◽  
Marker Compound ◽  
Skin Cells ◽  
Anti Cancer ◽  
Normal Human ◽  
Induced Apoptosis

Andrographis paniculata is traditionally used for many diseases and scientifically proven for anti-cancer property. Andrographolide which is the marker compound is believed to be the main contributor to the pharmacological activities. The poor solubility and bioavailability of this diterpenoid lactone could be overcome by nanoencapsulation. Reflux extraction, and followed by successive Soxhlet fractionation were used to obtain andrographolide rich extract from the herb. Spontaneous emulsion solvent diffusion was used to nanoencapsulate andrographolide using poly(lactic-co-glycolic acid) with 1% polyvinyl alcohol as emulsifier. Nanospheres loaded with andrographolide was found to have the particle size, 163 nm; polydispersity index, 0.26 and zeta potential, – 57.85 mV. The encapsulation efficiency and in vitro drug release were 80.0% and 84.2%, respectively. The andrographolide nanoparticles could inhibit the proliferation of cervical and neuroblastoma cells with no adverse effect on normal human skin cells. Andrographolide rich extract loaded nanoparticles could inhibit the proliferation of HeLa and SH-SY5Y cells, mainly through Bax-induced apoptosis. The result was consistent with the low expression of anti-apoptotic genes (Bcl-2 and Bcl-xL) and prognostic factor (Ki-67). The tumour size of HeLa bearing mice was significantly reduced (73%) after treated with andrographolide rich nanoparticles (10 mg/kg body weight) for a month.

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