scholarly journals New Helvolic Acid Derivatives with Antibacterial Activities from Sarocladium oryzae DX-THL3, an Endophytic Fungus from Dongxiang Wild Rice (Oryza rufipogon Griff.)

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1828
Author(s):  
Zhi-Bin Zhang ◽  
Si-Yao Du ◽  
Bo Ji ◽  
Chang-Jiu Ji ◽  
Yi-Wen Xiao ◽  
...  
Keyword(s):  
Endophytic Fungus ◽  
Wild Rice ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Oryza Rufipogon ◽  
Oryza Rufipogon Griff ◽  
Helvolic Acid ◽  
Dongxiang Wild Rice ◽  
Reported Data ◽  
New Compounds

Three new helvolic acid derivatives (named sarocladilactone A (1), sarocladilactone B (2) and sarocladic acid A (3a)), together with five known compounds (6,16-diacetoxy-25-hy- droxy-3,7-dioxy-29-nordammara-1,17(20)-dien-21-oic acid (3b), helvolic acid (4), helvolinic acid (5), 6-desacetoxy-helvolic acid (6) and 1,2-dihydrohelvolic acid (7)), were isolated from the endophytic fungus DX-THL3, obtained from the leaf of Dongxiang wild rice (Oryza rufipogon Griff.). The structures of the new compounds were elucidated via HR-MS, extensive 1D and 2D NMR analysis and comparison with reported data. Compounds 1, 2, 4, 5, 6 and 7 exhibited potent antibacterial activities. In particular, sarocladilactone B (2), helvolinic acid (5) and 6-desacetoxy-helvolic acid (6) exhibited strongly Staphylococcus aureus inhibitory activity with minimum inhibitory concentration (MIC) values of 4, 1 and 4 μg/mL, respectively. The structure–activity relationship (SAR) of these compounds was primarily summarized.

scholarly journals Identification, Analysis, and Confirmation of Seed Storability-Related Loci in Dongxiang Wild Rice (Oryza rufipogon Griff.)

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1831
Author(s):  
Minmin Zhao ◽  
Biaolin Hu ◽  
Yuanwei Fan ◽  
Gumu Ding ◽  
Wanling Yang ◽  
...  
Keyword(s):  
Wild Rice ◽  
Bulked Segregant Analysis ◽  
Oryza Rufipogon ◽  
Chromosome 9 ◽  
Rice Seed ◽  
Rice Varieties ◽  
Oryza Rufipogon Griff ◽  
Dongxiang Wild Rice ◽  
Candidate Loci ◽  
Seed Storability

Dongxiang wild rice (Oryza rufipogon Griff.) (DXWR) has strong seed storability and identifying its elite gene resources may facilitate genetic improvements in rice seed storability. In this study, we developed two backcross inbred lines (BILs) populations, with DXWR as a common donor parent and two rice varieties (F6 and R974) as recipient parents. Bulked segregant analysis via whole genome sequencing (BSA-seq) was used to identify seed storability-related loci in the DXWR and F6 population. Two main genomic regions containing 18,550,000–20,870,000 bp on chromosome 4 and 7,860,000–9,780,000 bp on chromosome 9 were identified as candidate loci of DXWR seed storability; these overlapped partially with seed storability-related quantitative trait loci (QTLs) discovered in previous studies, suggesting that these loci may provide important regions for isolating the responsible genes. In total, 448 annotated genes were predicted within the identified regions, of which 274 and 82 had nonsynonymous and frameshift mutations, respectively. We detected extensive metabolic activities and cellular processes during seed storability and confirmed the effects of the seed storability-related candidate loci using four BILs from DXWR and R974. These results may facilitate the cloning of DXWR seed storability-related genes, thereby elucidating rice seed storability and its improvement potential.

scholarly journals Molecular identification of the cytoplasmic male sterile source from Dongxiang wild rice (Oryza rufipogon Griff.)

2017 ◽  
Vol 16 (8) ◽  
pp. 1669-1675 ◽  
Author(s):  
Song YAN ◽  
Shan ZHU ◽  
Ling-hua MAO ◽  
Ren-liang HUANG ◽  
Hong-liang XIONG ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Oryza Rufipogon Griff ◽  
Dongxiang Wild Rice

scholarly journals Transcriptome Analysis of Salt Stress Responsiveness in the Seedlings of Dongxiang Wild Rice (Oryza rufipogon Griff.)

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146242 ◽  
Author(s):  
Yi Zhou ◽  
Ping Yang ◽  
Fenglei Cui ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transcriptome Analysis ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Oryza Rufipogon Griff ◽  
Stress Responsiveness ◽  
Dongxiang Wild Rice

scholarly journals Development and verification of SSR markers from drought stress-responsive miRNAs in common wild rice

2021 ◽  
Author(s):  
Yong Chen ◽  
Yuanwei Fan ◽  
Wanling Yang ◽  
Gumu Ding ◽  
Minmin Zhao ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ssr Markers ◽  
Wild Rice ◽  
Oryza Rufipogon ◽  
Rice Varieties ◽  
Common Wild Rice ◽  
Oryza Rufipogon Griff ◽  
Rice Chromosomes ◽  
Ssr Loci ◽  
Dongxiang Wild Rice

PREMISE: Dongxiang wild rice (Oryza rufipogon Griff., DXWR) is the northernmost common wild rice found in the world, which possesses abundant elite genetic resources. We developed a set of drought stress-responsive microRNA (miRNA)-based single sequence repeat (SSR) markers for DXWR, which will help breed drought stress-resistant rice varieties. METHODS AND RESULTS: Ninety-nine SSR markers were developed from the drought stress-responsive miRNAs of DXWR. The SSR loci were distributed in all 12 rice chromosomes and most were in chromosomes 2 and 6, with di- and trinucleotides being the most abundant repeat motifs. Nine out of ten synthesized SSR markers were displayed high levels of genetic diversity in the genomes of DXWR and 41 modern rice varieties worldwide. The number of alleles per locus ranged from 2 to 6, and the observed and expected heterozygosity ranged from 0.000 to 0.024 and 0.461 to 0.738, respectively. CONCLUSIONS: These SSR markers developed from drought stress-responsive miRNAs in DXWR could be additional tools for elite genes mapping and useful for drought stress-resistant rice breeding.

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