scholarly journals Antifungal Activity of Isolated Compounds from the Leaves of Combretum erythrophyllum (Burch.) Sond. and Withania somnifera (L.) Dunal against Fusarium Pathogens

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4732
Author(s):  
Hlabana Alfred Seepe ◽  
Tselane Geneva Ramakadi ◽  
Charity Mekgwa Lebepe ◽  
Stephen O. Amoo ◽  
Winston Nxumalo
Keyword(s):  
Antifungal Activity ◽  
Seed Germination ◽  
Withania Somnifera ◽  
Raw 264.7 ◽  
Antifungal Compounds ◽  
Maize Seed ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells ◽  
Fusarium Pathogens

Crop diseases caused by Fusarium pathogens, among other microorganisms, threaten crop production in both commercial and smallholder farming. There are increasing concerns about the use of conventional synthetic fungicides due to fungal resistance and the associated negative effects of these chemicals on human health, livestock and the environment. This leads to the search for alternative fungicides from nature, especially from plants. The objectives of this study were to characterize isolated compounds from Combretum erythrophyllum (Burch.) Sond. and Withania somnifera (L.) Dunal leaf extracts, evaluate their antifungal activity against Fusarium pathogens, their phytotoxicity on maize seed germination and their cytotoxicity effect on Raw 264.7 macrophage cells. The investigation led to the isolation of antifungal compounds characterized as 5-hydroxy-7,4′-dimethoxyflavone, maslinic acid (21-hydroxy-3-oxo-olean-12-en-28-oic acid) and withaferin A (4β,27-dihydroxy-1-oxo-5β,6β-epoxywitha-2-24-dienolide). The structural elucidation of the isolated compounds was established using nuclear magnetic resonance (NMR) spectroscopy, mass spectroscopy (MS) and, in comparison, with the available published data. These compounds showed good antifungal activity with minimum inhibitory concentrations (MIC) less than 1.0 mg/mL against one or more of the tested Fusarium pathogens (F. oxysporum, F. verticilloides, F. subglutinans, F. proliferatum, F. solani, F. graminearum, F. chlamydosporum and F. semitectum). The findings from this study indicate that medicinal plants are a good source of natural antifungals. Furthermore, the isolated antifungal compounds did not show any phytotoxic effects on maize seed germination. The toxicity of the compounds A (5-hydroxy-7,4′-dimethoxyflavone) and AI (4β,27-dihydroxy-1-oxo-5β,6β-epoxywitha-2-24-dienolide) was dose-dependent, while compound B (21-hydroxy-3-oxo-olean-12-en-28-oic acid) showed no toxicity effect against Raw 264.7 macrophage cells.

Anti-inflammatory Effects of Madecassic Acid via the Suppression of NF-κB Pathway in LPS-Induced RAW 264.7 Macrophage Cells

Planta Medica ◽  
2009 ◽  
Vol 76 (03) ◽  
pp. 251-257 ◽  
Author(s):  
Jong-Heon Won ◽  
Ji-Sun Shin ◽  
Hee-Juhn Park ◽  
Hyun-Ju Jung ◽  
Duck-Jae Koh ◽  
...  
Keyword(s):  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells ◽  
Anti Inflammatory

scholarly journals Indirect detection of superoxide in RAW 264.7 macrophage cells using microchip electrophoresis coupled to laser-induced fluorescence

2015 ◽  
Vol 407 (23) ◽  
pp. 7003-7012 ◽  
Author(s):  
Richard P. S. de Campos ◽  
Joseph M. Siegel ◽  
Claudia G. Fresta ◽  
Giuseppe Caruso ◽  
José A. F. da Silva ◽  
...  
Keyword(s):  
Microchip Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Indirect Detection ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

Haloperidol and Risperidone at high concentrations activate an in vitro inflammatory response of RAW 264.7 macrophage cells by induction of apoptosis and modification of cytokine levels

2015 ◽  
Vol 233 (9) ◽  
pp. 1715-1723 ◽  
Author(s):  
Ivo Emílio da Cruz Jung ◽  
Alencar Kolinski Machado ◽  
Ivana Beatrice Mânica da Cruz ◽  
Fernanda Barbisan ◽  
Verônica Farina Azzolin ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Cytokine Levels ◽  
Raw 264.7 Macrophage Cells ◽  
Induction Of Apoptosis ◽  
High Concentrations

Surfactants and fatty alcohol based novel nanovesicles for resveratrol: Process optimization, characterization and evaluation of functional properties in RAW 264.7 macrophage cells

2018 ◽  
Vol 261 ◽  
pp. 387-396 ◽  
Author(s):  
Jaya Sravani Vankayala ◽  
Surendra Nath Battula ◽  
Ruckmani Kandasamy ◽  
Gover Antoniraj Mariya ◽  
Magdaline Eljeeva Emerald Franklin ◽  
...  
Keyword(s):  
Process Optimization ◽  
Functional Properties ◽  
Fatty Alcohol ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

Use of rhodamine-allyl Schiff base in chemodosimetric processes for total palladium estimation and application in live cell imaging

2018 ◽  
Vol 42 (21) ◽  
pp. 17351-17358 ◽  
Author(s):  
Anup Kumar Bhanja ◽  
Snehasis Mishra ◽  
Ketaki Kar ◽  
Kaushik Naskar ◽  
Suvendu Maity ◽  
...  
Keyword(s):  
Schiff Base ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Living Cells ◽  
Live Cell ◽  
Raw 264.7 ◽  
Macrophage Cells ◽  
Raw 264.7 Macrophage ◽  
Raw 264.7 Macrophage Cells

An allyl-rhodamine Schiff base shows excellent palladium sensitivity (LOD, 95 nM) irrespective of Pd(0,ii,iv) and practical applicability is judged in living cells of RAW 264.7 (macrophage) cells.

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