scholarly journals Identification of Bacteria Associated with Post-Operative Wounds of Patients with the Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Approach

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5007
Author(s):  
Małgorzata Szultka-Młyńska ◽  
Daria Janiszewska ◽  
Paweł Pomastowski ◽  
Michał Złoch ◽  
Wojciech Kupczyk ◽  
...  
Keyword(s):  
Brain Heart Infusion ◽  
Culture Conditions ◽  
Clinical Samples ◽  
Bromocresol Purple ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Enterococcus Spp ◽  
Identification Of Bacteria ◽  
Staphylococcus Spp

The bacterial infection of post-operative wounds is a common health problem. Therefore, it is important to investigate fast and accurate methods of identifying bacteria in clinical samples. The aim of the study was to analyse the use of the MALDI-TOF MS technique to identify microorganism wounds that are difficult to heal. The most common bacteria are Escherichia coli, Staphylococcus spp., and Enterococcus spp. We also demonstrate the effect of culture conditions, such as the used growth medium (solid: Brain Heart Infusion Agar, Mueller Hilton Agar, Glucose Bromocresol Purple Agar, and Vancomycin Resistance Enterococci Agar Base and liquid: Tryptic Soy Broth and BACTEC Lytic/10 Anaerobic/F), the incubation time (4, 6, and 24h), and the method of the preparation of bacterial protein extracts (the standard method based on the Bruker guideline, the Sepsityper method) to identify factors and the quality of the obtained mass spectra. By comparing the protein profiles of bacteria from patients not treated with antibiotics to those treated with antibiotics based on the presence/absence of specific signals and using the UniProt platform, it was possible to predict the probable mechanism of the action of the antibiotic used and the mechanism of drug resistance.

scholarly journals Species Distribution and Antimicrobial Profiles of Enterococcus spp. Isolates from Kenyan Small and Medium Enterprise Slaughterhouses

2018 ◽  
Vol 81 (9) ◽  
pp. 1445-1449 ◽  
Author(s):  
JOSEPH WAMBUI ◽  
TAURAI TASARA ◽  
PATRICK MURIGU KAMAU NJAGE ◽  
ROGER STEPHAN
Keyword(s):  
Antibiotic Resistance Genes ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Small And Medium Enterprise ◽  
Beef Carcasses ◽  
Enterococcus Spp ◽  
Enterococcus Casseliflavus ◽  
Disk Diffusion Assay ◽  
Cutting Equipment ◽  
Medium Enterprise

ABSTRACT The present study aimed at identifying and assessing antimicrobial resistance of Enterococcus spp. isolated from small and medium enterprise slaughterhouses in Kenya. In total, 67 isolates were recovered from 48 of 195 samples examined from beef carcasses, personnel, and cutting equipment in five small and medium enterprise slaughterhouses. The isolates were identified by using matrix-assisted laser desorption–ionization time of flight mass spectrometry and screened thereafter for their resistance against 12 antibiotics by using a disk diffusion assay. The isolates (n = 67) included Enterococcus faecalis (41.8%), Enterococcus mundtii (17.9%), Enterococcus thailandicus (13.4%), Enterococcus faecium (9.0%), Enterococcus hirae (7.5%), Enterococcus casseliflavus (6.0%), and Enterococcus devriesei (4.5%). None of the isolates were resistant to ciprofloxacin, penicillin, ampicillin, vancomycin, nitrofurantoin, teicoplanin, linezolid, and levofloxacin. Resistance to rifampin (46.3%), erythromycin (23.9%), tetracycline (20.9%), and chloramphenicol (7.5%) was distributed among six of the seven species. All E. thailandicus were resistant to rifampin, erythromycin, and tetracycline. E. faecalis was resistant to rifampin (60.7%), tetracycline (17.9%), erythromycin (14.3%), and chloramphenicol (10.7%). Resistance to two or three antibiotics was observed in 26.9% of the enterococci isolates. The isolation of enterococci that are resistant to clinically relevant antibiotics, such as erythromycin, is of a serious concern given the role enterococci play in the transfer of antibiotic resistance genes.

scholarly journals Evaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Rapid Identification of Bacteria in Processed Soybean Products

2013 ◽  
Vol 2 (3) ◽  
pp. 104 ◽  
Author(s):  
Yuko Furukawa ◽  
Mitsuru Katase ◽  
Kazunobu Tsumura
Keyword(s):  
Laser Desorption ◽  
Rapid Identification ◽  
Bacterial Cells ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Identification Of Bacteria ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently been demonstrated as a rapid and reliable method for identifying bacteria in colonies grown on culture plates. Rapid identification of food spoilage bacteria is important for ensuring the quality and safety of food. To shorten the time of analysis, several researchers have proposed the direct MALDI-TOF MS tequnics for identification of bacteria in clinical samples such as urine and positive blood cultures. In this study, processed soybean products (total 26 test samples) were initially conducted a culture enrichiment step and bacterial cells were separated from interfering components. Harvested bacterial cells were determined by MALDI-TOF MS and 16S rRNA gene sequencing method. Six processed soybean products (23%) were increased bacterial cells after culture enrichiment step and they were sucessfully obtained the accurate identification results by MALDI-TOF MS-based method without colony formation.

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