Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture

The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire® 50/13 and Precirol® ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of hsp70 and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire® 50/13/Precirol® ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells.

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Synthesis and Characterization of Valacyclovir HCl Hybrid Solid Lipid Nanoparticles by Using Natural Oils

Recent Patents on Drug Delivery & Formulation ◽

10.2174/1872211313666190304142129 ◽

2019 ◽

Vol 13 (1) ◽

pp. 46-61

Author(s):

Archana Chacko ◽

Amaldoss M.J. Newton

Keyword(s):

Zeta Potential ◽

Solid Lipid Nanoparticles ◽

Grape Seed ◽

Lipid Nanoparticles ◽

Correlation Spectroscopy ◽

Good Stability ◽

Hybrid Nanoparticles ◽

Solid Lipid ◽

Natural Oils ◽

Grape Seed Oil

Background: The Jojoba Simmondsia Chinensis oil is used as one of the main ingredients which has an antioxidant, moisturizing and stabilizing activity. Likewise, grape seed (Vitis vinifera) oil is also used in this preparation which also has some remarkable medicinal properties such as antioxidant, astringent and is also used as a moisturizer. The Valacyclovir Solid Lipid Nanoparticles (SLN) are prepared in combination. Objective: The prime objective of the study was to prepare a nanodispersion with good stability indicating zeta potential. The formulations were prepared by varying concentrations of jojoba oil and grape seed oil which form the hybrid nanoparticles with the drug. Methods: The high-pressure hot-homogenization technique was used to prepare the nanoparticles. The prepared nanoparticles were subjected to characterization analysis such as Mean particle size, Zaverage, and Zeta potential by using Dynamic Light Scattering (DLS) and Photon Correlation Spectroscopy (PCS). The best formulation was subjected to Transmission Electron Microscopy (TEM) technique for surface morphology and other characterizations. The crystalline pattern of the drug alone, drug-loaded nanoparticles and nanoparticles without the drug was studied by XRD. The drug excipients compatibility studies were performed by using Fourier-Transform Infrared Spectroscopy (FTIR) Differential Scanning Calorimetry and (DSC). The other factors such as in vitro drug release, and % drug entrapment efficiency were studied by using suitable methods. Results: The results demonstrated that the particles are in nano range with good stability with appreciable Zeta potential (-48.2±mV). The selected formulations were analyzed for MPS which demonstrated the value of 306.7±183.4 and 416.5±289.3. The best formulation VNP5 demonstrated the Bellshaped curve and confirmed the uniform distribution. Conclusion: Based on the patents, it was demonstrated that valacyclovir is widely used in the treatment and prophylaxis of viral infections in human, particularly infections caused by the herpes group of viruses. Valacyclovir is an effective drug for the treatment of cold sores.

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Glutathione loaded solid lipid nanoparticles: Preparation and in vitro evaluation as delivery systems of the antioxidant peptide to immunocompetent fish cells

Journal of Cellular Biotechnology ◽

10.3233/jcb-15022 ◽

2016 ◽

Vol 2 (1) ◽

pp. 1-14 ◽

Cited By ~ 2

Author(s):

Adriana Trapani ◽

Giuseppe Tripodo ◽

Delia Mandracchia ◽

Nicola Cioffi ◽

Nicoletta Ditaranto ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

Delivery Systems ◽

Antioxidant Peptide ◽

In Vitro Evaluation ◽

Solid Lipid ◽

Fish Cells

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Glutathione-loaded solid lipid nanoparticles based on Gelucire® 50/13: Spectroscopic characterization and interactions with fish cells

Journal of Drug Delivery Science and Technology ◽

10.1016/j.jddst.2018.08.013 ◽

2018 ◽

Vol 47 ◽

pp. 359-366 ◽

Cited By ~ 4

Author(s):

Adriana Trapani ◽

Giuseppe Tripodo ◽

Delia Mandracchia ◽

Nicola Cioffi ◽

Nicoletta Ditaranto ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Spectroscopic Characterization ◽

Lipid Nanoparticles ◽

Solid Lipid ◽

Fish Cells

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Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line

Sustainability ◽

10.3390/su12187697 ◽

2020 ◽

Vol 12 (18) ◽

pp. 7697 ◽

Cited By ~ 1

Author(s):

Eliana B. Souto ◽

Patricia Severino ◽

Conrado Marques ◽

Luciana N. Andrade ◽

Alessandra Durazzo ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Cell Line ◽

Solid Lipid Nanoparticles ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Lipid Nanoparticles ◽

Human Neuroblastoma ◽

Solid Lipid ◽

Cetyl Palmitate

The essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus (CA) Kunth essential oil (CAEO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CAEO (CAEO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CAEO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN (CAEO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil (fEO) and loaded essential oil (CAEO-SLN) were demonstrated to inhibit the Fenton reaction. CAEO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity.

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Cyto/Biocompatibility of Dopamine Combined with the Antioxidant Grape Seed-Derived Polyphenol Compounds in Solid Lipid Nanoparticles

Molecules ◽

10.3390/molecules26040916 ◽

2021 ◽

Vol 26 (4) ◽

pp. 916

Author(s):

Adriana Trapani ◽

Lorenzo Guerra ◽

Filomena Corbo ◽

Stefano Castellani ◽

Enrico Sanna ◽

...

Keyword(s):

Solid Lipid Nanoparticles ◽

Chemical Characterization ◽

Grape Seed ◽

Lipid Nanoparticles ◽

Non Invasive ◽

Solid Lipid ◽

Physico Chemical ◽

Release Studies ◽

Nigrostriatal Neurons ◽

6 Hydroxydopamine

Background: The loss of nigrostriatal neurons containing dopamine (DA) together with the “mitochondrial dysfunction” in midbrain represent the two main causes related to the symptoms of Parkinson’s disease (PD). Hence, the aim of this investigation is to co-administer the missing DA and the antioxidant grape seed-derived proanthocyanidins (grape seed extract, GSE) in order to increase the levels of the neurotransmitter (which is unable to cross the Blood Brain Barrier) and reducing the oxidative stress (OS) related to PD, respectively. Methods: For this purpose, we chose Solid Lipid Nanoparticles (SLN), because they have been already proven to increase DA uptake in the brain. DA-SLN adsorbing GSE (GSE/DA-SLN) were formulated and subjected to physico-chemical characterization, and their cytocompatibility and protection against OS were examined. Results: GSE was found on SLN surface and release studies evidenced the efficiency of GSE in preventing DA autoxidation. Furthermore, SLN showed high mucoadhesive strength and were found not cytotoxic to both primary Olfactory Ensheathing and neuroblastoma SH-SY5Y cells by MTT test. Co-administration of GSE/DA-SLN and the OS-inducing neurotoxin 6-hydroxydopamine (100 μM) resulted in an increase of SH-SY5Y cell viability. Conclusions: Hence, SLN formulations containing DA and GSE may constitute interesting candidates for non-invasive nose-to-brain delivery.

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