scholarly journals Chemical Profile, Antimicrobial and Antioxidant Activity Assessment of the Crude Extract and Its Main Flavonoids from Tartary Buckwheat Sprouts

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 374
Author(s):  
Lingyun Zhong ◽  
Yuji Lin ◽  
Can Wang ◽  
Bei Niu ◽  
Ying Xu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Spore Germination ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Pathogenic Fungi ◽  
Tartary Buckwheat ◽  
Strong Activity ◽  
Ic50 Value

The purpose of this study was to investigate the major flavonoids content and bioactivities of Tartary buckwheat sprouts. The crude methanol extract (ME) of Tartary buckwheat sprouts was abundant in flavonoids, and six major flavonoids, including isoorientin, vitexin, isovitexin, rutin, quercetin, and kaemferol were successfully determined from the sprouts by the high-performance liquid chromatography (HPLC) method. Generally, the flavonoid content of buckwheat sprouts was in the order of rutin > quercetin > isovitexin > vitexin> isoorientin > kaemferol. The highest rutin content of the ME and sprout cultures was 89.81 mg/g and 31.50 mg/g, respectively. Antibacterial activity results indicated the ME displayed notable inhibitory activity against the five tested bacteria, and its minimum inhibitory concentration (MIC) values ranged from 0.8 mg/mL to 3.2 mg/mL. Among the six flavonoids, quercetin was the most active compound, which exhibited strong activity against all tested bacteria except for E. coli and S. epidermidis, with its MIC values ranging from 0.2 mg/mL to 0.4 mg/mL. For the antifungal activity assay, the ME of Tartary buckwheat sprouts and four flavonoids could significantly inhibit the spore germination of two pathogenic fungi, and their inhibitory efficiency was concentration dependent. Quercetin was the most active one, which significantly inhibited the spore germination of F. oxysporum f. sp. vasinfectum and F. oxysporum f. sp. cucumerinum, and its median effective inhibitory concentration (IC50) value was 42.36 and 32.85 µg/mL, respectively. The antioxidant activity results showed that quercetin, kaemferol, and rutin displayed excellent antioxidant activity in the DPPH radical scavenging test, and their IC50 value was calculated as 5.60, 16.23, and 27.95 µg/mL, respectively. This is the first report on the antimicrobial activity of the crude extract of Tartary buckwheat sprouts. These results indicated that the methanol extract of Tartary buckwheat sprouts could be used as a potential antimicrobial or antioxidant agent in the future.

Phytochemical Screening, Total Phenolics Contents and in vitro Antioxidant Activity of Salvia officinalis, Satureja calamintha, Mentha pulegium and Marrubium vulgare

2021 ◽  
Author(s):  
N. Belkacem ◽  
R. Azzi ◽  
R. Djaziri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Crude Extract ◽  
Condensed Tannins ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Salvia Officinalis ◽  
Phytochemical Analysis ◽  
Aqueous Methanol ◽  
Mentha Pulegium

This study was designed to evaluate the antioxidant activity of phenolic compounds of for Lamiaceae: Salvia officinalis, Satureja calamintha, Mentha pulegium and Marubium vulgare using ferric reducing power (FRAP) and radical scavenging method (DPPH). Preliminary phytochemical analysis shows that the major constituents of crude extracts (Hydromethanolic, hydroacetonic) are tannins, saponins, quinones, flavonoids, terpenoids and reducing compounds in the four plants. In quantitative analysis, variable polyphenols, flavonoids and condensed tannins content are recorded. The highest polyphenols content is that of the aqueous/methanol crude extract of Salvia officinalis (188.92 ± 1.19 mg GAE/ gE), followed by aqueous/acetone crude extract of Mentha pulegium (176.55 ± 2.49 mg GAE/gE). Flavonoids and condensed tannins content of crude extract showing a high content in aqueous/methanol extract of Mentha pulegium and Satureja calamintha (93.64 ± 0.26mg EQC/gE and 25.44 ± 0.71 mg EQC/gE), respectively. Scavenging activity of the free radical DPPH, revealed that the aqueous/methanol extract of Salvia officinalis shown a relatively high antioxidant activity (IC50 = 1.07 μg/ml) compared with ascorbic acid (IC50 = 0.91 μg/ml). FRAP method revealed that the extracts of Mentha pulegium showed the highest power to reduce iron (EC50 = 0.147 mg/ml), but lower than that of ascorbic acid (EC50 = 0.057 mg/mL).

Antioxidant and Anti-Melanogenic Activities of Pakoba Leaves (Syzygium sp) from North Celebes

2019 ◽  
Vol 2 (2) ◽  
pp. 79-83
Author(s):  
Dela Rosa ◽  
Catherine Roeroe ◽  
Agustina Susanti
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Chloroform Fraction ◽  
Aqueous Fraction ◽  
Ic50 Value ◽  
Dpph Method ◽  
Good Potential ◽  
Crude Methanol Extract

In this research the potential of Pakoba leaf extract to be used as antioxidant and skin whitening (anti-melanogenic agent) is investigated. The antioxidant activity of Pakoba leaves were studied using DPPH method and the result showed that the 80 percent methanol crude extract has strong antioxidant activity with an IC50 value of 22.66 ± 1.02 μg/ml. The aqueous fraction of the sample has an IC50 value of 53.30 ± 1.42 μg/ml, followed by n-butanol fraction (53.63 ± 1.45 μg/ml) and chloroform fraction (511.54 ± 1.59 μg/ml). The anti-melanogenic activity of the crude methanol extract showed IC50 value of 316.56 ± 11.04 μg/ml. Thus, it isconcluded that crude extract of Pakoba leaves shows good potential as the antioxidant source although it does not show good anti- melanogenic activity.

scholarly journals Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

2019 ◽  
Vol 40 ◽  
pp. 30-35
Author(s):  
Ishwor Pathak ◽  
Muna Niraula
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF TROPICAL BROWN ALGAE Padina australis FROM PRAMUKA ISLAND, DISTRICT OF SERIBU ISLAND, INDONESIA

2013 ◽  
Vol 5 (2) ◽  
Author(s):  
Joko Santoso ◽  
Fitriany Podungge ◽  
Heru Sumaryanto
Keyword(s):  
Antioxidant Activity ◽  
Dietary Fiber ◽  
Proximate Composition ◽  
Nutritional Value ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Ic50 Value ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

The proximate composition, dietary fiber, and total phenol contents as well as antioxidant activity of tropical brown alga Padina australis collected from the Pramuka Island, District of Seribu Island, Indonesia during the rainy season of 2011 were determined in order to evaluate their potential nutritional value and activity of natural antioxidant compound. The content of ash, protein, and fat were 22.26, 10.76, and 4.17 g/100 g dry matter, respectively; whereas the amounts of soluble, insoluble, and total dietary fibers were 8.4, 5.4, and 13.8 g/100 g, respectively. Methanol extract of P. australis contained the highest total phenol of 246.1 mg GAE/1000 g dry sample. The extract also had the highest activity on DPPH radical scavenging, measured by IC50 of 267.1 ppm. Both the total phenol and IC50 value extracts decreased in the following order: methanol > ethyl acetate > n-hexane.Keywords: antioxidant, dietary fiber, DPPH-scavenging, Padina australis, proximate composition

scholarly journals Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Fafa Nurdyansyah ◽  
Dyah Ayu Widyastuti
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Water Extract ◽  
Bark Extract ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Rotary Vacuum ◽  
Potential Content

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50

scholarly journals Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

2019 ◽  
Vol 14 (2) ◽  
pp. 73
Author(s):  
Wendy Alexander Tanod ◽  
Didit Kustantio Dewanto ◽  
Samliok Ndobe ◽  
Putut Har Riyadi ◽  
Masteria Yunovilsa Putra
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Soft Corals ◽  
Strong Activity ◽  
E Coli ◽  
Central Sulawesi

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

scholarly journals Aktivitas Antioksidan Ekstrak Daun Mangrove Rhizopora mucronata

2017 ◽  
Vol 6 (2) ◽  
pp. 110 ◽  
Author(s):  
Ali Ridlo ◽  
Rini Pramesti ◽  
Koesoemadji Koesoemadji ◽  
Endang Supriyantini ◽  
Nirwani Soenardjo
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Chlorophyll A ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Ic50 Value

Rhizopora mucronata merupakan salah satu jenis mangrove yang berpotensi  sebagai sumber antioksidan alami. Daun tanaman ini mengandung senyawa metabolit sekunder seperti tanin, fenolat, klorofil, karotenoid dan alkaloid. Penelitian ini bertujuan menentukan aktivitas antioksidan ekstrak daun R. mucronata. Sampel diambil dari kawasan mangrove Tugurejo, Semarang dan diekstraksi  secara bertingkat berturut-turut dengan pelarut n-heksana, etil asetat dan metanol. Aktivitas antioksidan diukur dengan metode DPPH (1,1-diphenyl-2-picrylhidrazyl) dan nilainya ditentukan berdasarkan nilai Inhibitory Concentration (IC50)pada panjang gelombang 516,5 nm. Kadar senyawa fenolat total ditentukan secara spektrofotometri pada panjang gelombang 725 nm dengan metode Folin-Ciocalteu, kadar klorofil a dan b ditentukan dengan metode spektrofotometri pada panjang gelombang 663 nm dan 645 nm dan kadar karotenoid diukur pada 480 nm. Hasil penelitian menunjukkan ekstrak metanol memiliki nilai IC50terkecil (113,41  ppm), diikuti ekstrak n-heksana 151,13 ppm dan ekstrak etil asetat 184,78 ppm. Kandungan total fenolat tertinggi terdapat pada ekstrak metanol yaitu 21,06 mg GAE/g sampel, ekstrak n-heksana 13,27 mg GAE/g sampel dan ekstrak etil asetat 2,08 mg GAE/g sampel. Kandungan klorofil a tertinggi terdapat pada ekstrak metanol yaitu 2,304  mg/g, diikuti ekstrak n-heksana 0,705 mg/g dan ekstrak etil asetat 0,64 mg/g. Kandungan klorofil b tertinggi dicapai ekstrak metanol yaitu 0,97 mg/g, ekstrak n-heksana 0,50 mgg dan ekstrak etil asetat 0,13 mg/g. Kandungan karotenoid tertinggi dicapai pada ekstrak metanol yaitu 6,49 mg GAE/g, diikuti ekstrak etil asetat (0.54 mg GAE/g) dan ekstrak n-heksana (1,37 mg GAE/g). Ekstrak metanol memiliki aktivitas antioksidan tertinggi dan termasuk dalam antioksidan kategori sedang, sedangkan ekstrak etil asetat dan  n-heksana termasuk dalam  antioksidan kategori lemah.   Rhizopora mucronata is one type of mangrove that has the potential as a source of natural antioxidants. The leaves of this plant contain secondary metabolite compounds such as tannins, phenolics, chlorophyll, carotenoids and alkaloids. This study aims to determine the antioxidant activity of R. mucronata leaf extract. Samples were taken from Tugurejo mangrove area, Semarang and extracted successively with n-hexane, ethyl acetate and methanol solvent. Antioxidant activity was measured by DPPH (1,1-diphenyl-2-picrylhidrazyl) method and its value was determined based on Inhibitory Concentration (IC50) value at 516,5 nm wavelength. Total phenolic compound concentration was determined spectrophotometrically at 725 nm wavelength with Folin-Ciocalteu method. The levels of chlorophyll a and b was determined by spectrofotometry method at 663 nm and 645 nm wavelength and carotenoid levels were measured at 480 nm. The results showed that methanol extract had the smallest IC50 value (113,41 ppm), followed by n-hexane extract 151,13 ppm and ethyl acetate extract 184,78 ppm. The highest total phenolic content was found in methanol extract, 21.06 mg GAE / g sample, n-hexane extract 13,27 mg GAE / g sample and ethyl acetate extract 2.08 mg GAE / g sample. The highest content of chlorophyll a contained in methanol extract is 2,304 mg / g, followed by n-hexane extract 0,705 mg / g and ethyl acetate extract 0,64 mg / g. The highest content of chlorophyll b was methanol extract of 0.97 mg / g, n-hexane extract 0,50 mgg and ethyl acetate extract 0,13 mg / g. The highest carotenoid content was achieved in the methanol extract of 6.49 mg GAE / g, followed by ethyl acetate extract (0.54 mg GAE / g) and n-hexane extract (1.37 mg GAE / g). Methanol extract has the highest antioxidant activity and is included in medium category antioxidants, while ethyl acetate and n-hexane extracts are included in weak category antioxidants. 

scholarly journals EFFECT OF INDOLE -3- ACETIC ACID ON CALLUS INDUCTION FROM LEAF EXPLANT OF OCIMUM BASILICUM LINN.

2015 ◽  
Vol 2 (2) ◽  
pp. 146-150
Author(s):  
Shillpa Sree V.N ◽  
Sirangeevi V ◽  
Deeksha S ◽  
Bhuvaneswari G ◽  
Ramya G ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Ocimum Basilicum ◽  
Dpph Radical ◽  
Ic50 Value ◽  
The Family ◽  
Half Strength ◽  
Dpph Radical Scavenging ◽  
Indole 3 Acetic Acid

Ocimum basilicum L. belongs to the family Lamiaceae; contains plenty of phytochemicals with significant nutritional as well as health benefits. Antioxidant activity of methanol extract of Ocimum basilicum callus was evaluated using various antioxidant assays. Proliferated callus was obtained on half-strength MS media supplemented with 1.40µM IAA. The callus extract showed considerable DPPH radical scavenging, hydroxyl radical scavenging and ferrous ion chelation activity with IC50 value of 16.05, 170.7 and 29.16µg/mL respectively.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Daun Karet Kebo (Ficus Elastica) dengan Metode Peredaman Radikal Bebas Dpph (1,1-Diphenyl-2-Picrylhydrazil)

2020 ◽  
Vol 6 (1) ◽  
pp. 141-150
Author(s):  
Selpida Handayani ◽  
Ida Kurniawati ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Water Extract ◽  
Free Radical Scavenging ◽  
Water Extracts ◽  
Activity Test ◽  
Yield Value ◽  
Ic50 Value ◽  
Tlc Plates

Ficus elastica leaf is one of the Ficus species assumed to be rich in polyphenols so that it can act as an antioxidant. The research aimed to determine the antioxidant activity and IC50 value of methanol and water extracts of rubber leaf. In this research, the extraction was conducted by maceration using methanol and obtained the yield value of 7,03%, while the infundation using water obtained the yield value of 2,82%. The antioxidant activity test was conducted by DPPH free radical scavenging (1,1-diphenyl-2-phycrilhydrazyl) using a quercetin comparator. The qualitative tests were conducted by TLC method using dicloromethane:methanol eluent with a ratio of 99:1 for methanol extracts, and a ratio of 95:5 for water extracts, where all extracts of both methanol and water positively container antioxidants characterized by the formation of yellow spots with a purple background on TLC plates. The results of the quantitative test shower that the methanol extract had medium antioxidant activity with an IC50 value of 78,39 µg/mL, and water extract had an inactive antioxidant activity with an IC50 value of 319,11 µg/mL. While quercetin was classified as a very strong antioxidant with an IC50 value of 7,62 µg/mL.  

scholarly journals Phytochemical analysis and antioxidant activity of Haloxylon salicarnicum, Ochradenus arabicus and Tamarix nilotica

2019 ◽  
pp. 47-50
Author(s):  
Mariam M. A. Alshamsi ◽  
Alghazal K. H. Alnuaimi ◽  
A. Senthilkumar ◽  
Reema M. S. Alshamsi ◽  
K. Karthishwaran ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Volatile Oils ◽  
Ic50 Value ◽  
Ic50 Values

In the present investigation, the preliminary phytochemical analysis and antioxidant potential of hexane, chloroform, acetone and methanol extracts of Haloxylon salicarnicum, Ochradenus arabicus and Tamarix nilotica were reported. The presence of flavonoid, alkaloid, phenols, terpenoids, carbohydrate, tannin, protein, steroids, saponin, phlobatannin, cardiacglycoside, anthraquinines and volatile oils were determined and for antioxidant activity, DPPH, ABTS, superoxide anion radical and hydroxyl radical scavenging potential were studied. Strong presence of alkaloid, tannin, steroids in H. salicarnicum, flavonoid and phenols in O. arabicus and saponin T. nilotica were observed. In the present investigation, all the extracts inhibited the free radicals the methanol extract of O. arabicus was identified as potential crude extract compared to all other extracts with the IC50 values of 91.65 (DPPH), 94.62 (ABTS), 95.82 (O2) and 96.02 (OH) µg/mL. Whereas, the IC50 value of the standard, Gallic acid were 125.25 (DPPH), 142.32 (ABTS), 130.78 (O2) and 139.93 µg/mL (OH). Whereas, the IC50 value of the standard, Gallic acid were 125.25 (DPPH), 142.32 (ABTS), 130.78 (O2) and 139.93 µg/mL (OH)The present study suggests that methanol extract of O. arabicus can be used for the isolation potential natural antioxidant.

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