Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic Zingiber officinale Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak

In the present study, Zingiber officinale is used for the synthesis of Zingiber officinale capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs, lyophilized miswak, and chlorhexidine diacetate (CHX) against oral microbes. Five groups of the disc-shaped GIC specimens were prepared. Group A: lyophilized miswak and GIC combination, Group B: ZOE-AgNPs and GIC combinations, Group C: CHX and GIC combination, Group D: ZOE-AgNPs + CHX + GIC; Group E: Conventional GIC. Results confirmed the successful formation of ZOE-AgNPs that was monitored by UV-Vis sharp absorption spectra at 415 nm. The X-ray diffractometer (XRD) and transmission electron microscope (TEM) results revealed the formation of ZOE-AgNPs with a mean size 10.5–14.12 nm. The peaks of the Fourier transform infrared spectroscopy (FTIR) were appearing the involvement of ZOE components onto the surface of ZOE-AgNPs which played as bioreducing, and stabilizing agents. At a 24-h, one-week and three-week intervals, Group D showed the significantly highest mean inhibitory zones compared to Group A, Group B, and Group C. At microbe-level comparison, Streptococcus mutans and Staphylococcus aureus were inhibited significantly by all the specimens tested except group E when compared to Candida albicans. Group D specimens showed slightly higher (45.8 ± 5.4) mean compressive strength in comparison with other groups. The combination of GIC with ZOE-AgNPs and chlorhexidine together enhanced its antimicrobial efficacy and compressive strength compared to GIC with ZOE-AgNPs or lyophilized miswak or chlorhexidine combination alone. The present study revealed that The combination of GIC with active components of ZOE-AgNPs and chlorhexidine paves the way to lead its effective nano-dental materials applications.

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Base Materials’ Influence on Fracture Resistance of Molars with MOD Cavities

Materials ◽

10.3390/ma14185242 ◽

2021 ◽

Vol 14 (18) ◽

pp. 5242

Author(s):

Gabriela Ciavoi ◽

Ruxandra Mărgărit ◽

Liana Todor ◽

Dana Bodnar ◽

Magdalena Natalia Dina ◽

...

Keyword(s):

Fracture Resistance ◽

Glass Ionomer Cement ◽

Base Material ◽

Glass Ionomer ◽

Base Materials ◽

Significant Difference ◽

Group A ◽

Group B ◽

Group D ◽

Ionomer Cement

The aim of this study was to compare fracture resistance of teeth presenting medium-sized mesial-occlusal-distal (MOD) cavities using different base materials. Thirty-six extracted molars were immersed for 48 h in saline solution (0.1% thymol at 4 °C) and divided into six groups. In group A, the molars were untouched, and in group B, cavities were prepared, but not filled. In group C, we used zinc polycarboxylate cement, in group D—conventional glass ionomer cement, in group E—resin modified glass ionomer cement, and in group F—flow composite. Fracture resistance was tested using a universal loading machine (Lloyd Instruments) with a maximum force of 5 kN and a crosshead speed of 1.0 mm/min; we used NEXYGEN Data Analysis Software and ANOVA Method (p < 0.05). The smallest load that determined the sample failure was 2780 N for Group A, 865 N for Group B, 1210 N for Group C, 1340 N for Group D, 1630 N for Group E and 1742 N for Group F. The highest loads were 3050 N (A), 1040 N (B), 1430 N (C), 1500 N (D), 1790 N (E), and 3320 N (F), the mean values being 2902 ± 114 N (A), 972 ± 65 N (B), 1339 ± 84 N (C), 1415 ± 67 N (D), 1712 ± 62 N (E), and 2334 ± 662 N (F). A p = 0.000195 shows a statistically significant difference between groups C, D, E and F. For medium sized mesial-occlusal-distal (MOD) cavities, the best base material regarding fracture resistance was flow composite, followed by glass ionomer modified with resin, conventional glass ionomer cement and zinc polycarboxylate cement. It can be concluded that light-cured base materials are a better option for the analyzed use case, one of the possible reasons being their compatibility with the final restoration material, also light-cured.

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Assessment of the Antibacterial Property of Mineral Trioxide Aggregate and Glass Ionomer Cement Mixture

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i2730856 ◽

2020 ◽

pp. 58-64

Author(s):

Ankita Komal Labh ◽

Anjaneyulu K. ◽

Muralidharan N. P.

Keyword(s):

Antibacterial Property ◽

Glass Ionomer Cement ◽

Mineral Trioxide Aggregate ◽

Diffusion Method ◽

Filling Material ◽

Glass Ionomer ◽

Group A ◽

Group B ◽

Group D ◽

Ionomer Cement

Mineral trioxide aggregate (MTA) is an experimental material used for the capping of pulps as well as a root end filling material. Glass ionomer cement (GIC) is a widely used dental restorative due to its high mechanical strength and antibacterial function. The aim of this study is to assess the antibacterial property of MTA when mixed with GIC. The study comprised four groups with 5 samples each: Group A (MTA), group B (GIC), groups C (MTA+GIC+MTA Liquid) and group D (MTA+GIC+GIC Liquid). All samples were tested against Enterococcus faecalis using Agar well diffusion method in Tryptone soya agar. The One-way ANOVA test was employed using the Statistical Package for Social Sciences (SPSS) for MacOS (Version 19, 2010) with the level of significance set at P<0.05. The average zone of inhibition of group A was 18.6 ± 1.714 mm, group B was 0.4 ± 0.548 mm, group C was 18.6 ± 2.608 mm and group D was 12.8 ± 2.168 mm. Data was statistically significant, F(3,16)=11.631, P<0.001. The antibacterial activity of MTA and MTA when mixed with GIC and MTA liquid is similar but better than other study groups.

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SEALING ABILITY OF MINERAL TRIOXIDE AGGREGATE, CALCIUM PHOSPHATE CEMENT, AND GLASS IONOMER CEMENT IN THE REPAIR OF FURCATION PERFORATIONS

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2014.16 ◽

2013 ◽

Vol 56 (3) ◽

pp. 97-103 ◽

Cited By ~ 3

Author(s):

Prabath Singh ◽

Joseph Paul ◽

Abdul Aziz Al-Khuraif ◽

Sajith Vellappally ◽

Hassan Suliman Halawany ◽

...

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphate Cement ◽

Glass Ionomer Cement ◽

Mineral Trioxide Aggregate ◽

Glass Ionomer ◽

Dye Penetration ◽

Sealing Ability ◽

Group A ◽

Group B ◽

Ionomer Cement

Objectives: The purpose of this study was to evaluate the in vitro sealing ability of three repair materials. Mineral trioxide aggregate (MTA; Group A), calcium phosphate cement (CPC; Group B), and light cured glass ionomer cement (GIC; Group C) when used to repair the perforation created in the pulpal floor of fifty extracted human permanent molars. Materials and methods: Preparation of access openings and furcation perforations were done, and the teeth divided into five experimental groups (A, B, C) including two controls (D, E) with ten samples in each group randomly. Following the repair procedure, the pulp chambers and access openings were filled with composite resin and immersed in 2% methylene blue solution for 48 hours. The teeth were sectioned longitudinally and the linear dye penetration measured under a stereomicroscope. Results: The comparison of the linear length of micro-leakage (mm) among the experimental groups revealed no significant difference (p = 0.332). On calculating the percentage of depth of leakage to the total length of the perforation, it was observed that the mean leakage was 35.5% in Group A, 53.6% in Group B and the highest, 87.5% in Group C. The mean of leakage percentage was statistically significant by Kruskal-Wallis test (p = 0.003). The results indicated that the dye penetration used as furcation perforation repair material was least with mineral trioxide aggregate. Comparing the depth of penetration of dye, 50% of the Group A samples showed less than 25% of depth penetration. While 40% of Group B cases had more than 50% dye penetration. In our study, all Group C teeth had ≥ 50% dye penetration. Conclusions: The present study indicated that GIC had the greatest dye penetration followed by CPC and MTA. Mineral trioxide aggregate and calcium phosphate cement had comparatively better sealing ability than glass ionomer cement.

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Biosynthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against studied microorganisms and its effect on compressive strength

10.21203/rs.3.rs-35758/v2 ◽

2020 ◽

Author(s):

Enas Tawfik Enan ◽

Amal A. Ashour ◽

Sakeenabi Basha ◽

Nayef H. Felemban ◽

sanaa M. F. gad El-Rab

Keyword(s):

Antimicrobial Activity ◽

Compressive Strength ◽

Antimicrobial Agents ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Visible Spectrum ◽

Test System ◽

Glass Ionomer ◽

Group D ◽

Ionomer Cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.

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Antimicrobial Efficacy of Cinnamon Bark Oil on Lactobacillus acidophilus and its Effect on Compressive Strength of Glass Ionomer Cement

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1432 ◽

2017 ◽

Vol 8 (3) ◽

pp. 164-170

Author(s):

Sheetal Bhanushali

Keyword(s):

Compressive Strength ◽

Minimum Inhibitory Concentration ◽

Lactobacillus Acidophilus ◽

Inhibitory Concentration ◽

Glass Ionomer Cement ◽

Antimicrobial Efficacy ◽

Glass Ionomer ◽

Liquid Component ◽

Cinnamon Bark ◽

Ionomer Cement

ABSTRACT Aim The aim of this study is to evaluate the minimum inhibitory concentration (MIC) of cinnamon bark oil against Lactobacillus acidophilus and incorporate it in the liquid component of glass ionomer cement (GIC) followed by determination of its effect on the compressive strength. Materials and methods Antibacterial effect of various concentrations of cinnamon bark oil was evaluated using broth microdilution method with 96-well tissue culture plate. Minimum inhibitory concentration of the cinnamon bark oil against L. acidophilus was determined and that concentration was then incorporated into the liquid component of the GIC, and its compressive strength was evaluated. For compressive strength testing, teflon mold of 4 mm diameter and 6 mm height was used for the preparation of samples. The prepared specimens were stored in distilled water at 37°C for 24 hours in an incubator. After 24 hours, the samples were subjected to the universal testing machine at a speed of 1 mm/minute for compressive strength evaluation. The maximum load required to fracture the specimen was recorded. Results A volume of 20 ìL/mL was found to be the MIC of the cinnamon bark oil against L. acidophilus. The mean compressive strengths of conventional and cinnamon bark oil-incorporated GIC revealed no significant difference. Conclusion Cinnamon bark oil has antibacterial property against L. acidophilus. Incorporation of 2% v/v cinnamon bark oil did not adversely affect the compressive strength of GIC. Clinical significance By incorporating this bacteriostatic agent to GIC, the progress of caries and failure of restorations can be prevented by inhibiting the growth of L. acidophilus. Clinically, it can be used in cases of deep dentinal caries, early childhood caries, rampant caries, and patients with high caries index. How to cite this article Bhanushali S, Srilatha KT, Girish MS. Antimicrobial Efficacy of Cinnamon Bark Oil on Lactobacillus acidophilus and its Effect on Compressive Strength of Glass Ionomer Cement. World J Dent 2017;8(3):164-170.

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Evaluating the Mechanical Properties, and Calcium and Fluoride Release of Glass-Ionomer Cement Modified with Chicken Eggshell Powder

Dentistry Journal ◽

10.3390/dj6030040 ◽

2018 ◽

Vol 6 (3) ◽

pp. 40

Author(s):

Gehan Allam ◽

Ola Abd El-Geleel

Keyword(s):

Mechanical Properties ◽

Calcium Release ◽

Glass Ionomer Cement ◽

Fluoride Release ◽

Glass Ionomer ◽

Ion Release ◽

Chicken Eggshell ◽

Group A ◽

Group B ◽

Ionomer Cement

The aim of this study was to test the effect of adding chicken eggshell powder (CESP) to conventional glass-ionomer cement (GIC) on its mechanical properties, and fluoride and calcium release. CESP was added with proportions of 3% and 5% by weight to the powder component of conventional glass-ionomer cement. The specimens were categorized into group A: GIC without CESP; group B: GIC with 3% wt. CESP; and group C: GIC with 5% wt. CESP; there were 12 specimens in each group. Groups B and C showed higher compressive strength values compared to group A. However, microhardness scores were higher in group C compared to groups A and B. As for ion-release results, group B displayed the highest values of fluoride release followed by group C at both 7 and 30 days. Group C showed the highest amount of calcium release followed by both groups B and C at 7 days, while at 30 days, groups A and B showed higher calcium release compared to group C. The mechanical properties of conventional glass-ionomer restorative material were enhanced by the addition of CESP. Moreover, fluoride and calcium release were not compromised by adding CESP.

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Green synthesis and application of silver nano-biotics for enhancing the antimicrobial activity of glass-ionomer cement against dental biofilm and its effect on compressive strength

10.21203/rs.3.rs-35758/v1 ◽

2020 ◽

Author(s):

sanaa M. F. gad El-Rab ◽

Enas Tawfik Enan ◽

Amal A. Ashour ◽

Sakeenabi Basha ◽

Nayef H. Felemban

Keyword(s):

Antimicrobial Activity ◽

Compressive Strength ◽

Antimicrobial Agents ◽

Glass Ionomer Cement ◽

Antimicrobial Properties ◽

Test System ◽

Glass Ionomer ◽

Dental Biofilm ◽

Group D ◽

Ionomer Cement

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of their failure. The urge for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents. Objectives Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength of GIC was also evaluated. Material and methods Biosynthesis of AgNPs was done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity. The distribution of biofilm was examined via scanning electron microscope. Compressive strength (CS) of the tested material was measured using a Material Test System (MTS). Results UV-Visible spectrum showed a peak of 429 nm.TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5–25.8 nm. The maximum inhibitory zone was recorded for group D against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus) bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused insignificant effect on compressive strength of GIC. Conclusion Cupressus macrocarpa extract biosynthesized AgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against dental biofilm. The agents can be safely added without impairing mechanical properties of the set cement.

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