scholarly journals Dual-Channel Probe of Carbon Dots Cooperating with Lanthanide Complex Employed for Simultaneously Distinguishing and Sequentially Detecting Tetracycline and Oxytetracycline

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 128
Author(s):  
Lei Jia ◽  
Zhitao Xu ◽  
Rujie Chen ◽  
Xiangzhen Chen ◽  
Jun Xu
Keyword(s):  
Antimicrobial Agents ◽  
Fluorescence Probe ◽  
Color Change ◽  
Logic Gate ◽  
Lanthanide Complex ◽  
High Sensitivity ◽  
Inner Filter Effect ◽  
Antenna Effect ◽  
Buffer Solution ◽  
Dual Channel

Tetracycline (TC) and oxytetracycline (OTC) are the most widely used broad-spectrum antimicrobial agents in tetracycline drugs, and their structures and properties are very similar, so it is a great challenge to distinguish and detect these two antibiotics with a single probe at the same time. Herein, a dual-channel fluorescence probe (SiCDs@mMIPs-cit-Eu) was developed by integrating two independent reaction sites with SiCDs-doped mesoporous silica molecular imprinting group and europium complex group into a nanomaterial. The synergistic influence of inner filter effect and “antenna effect” can be guaranteed to solve the distinction between TC and OTC. Moreover, this novel strategy can also sequentially detect TC and OTC in buffer solution and real samples with high sensitivity and selectivity. This method revealed good responses to TC and OTC ranging from 0 to 5.5 μM with a detection limit of 5 and 16 nM, respectively. Combined with the smartphone color-scanning application, the portable and cheap paper-based sensor was designed to realize the multi-color visual on-site detection of TC and OTC. In addition, the logic gate device was constructed according to the fluorescence color change of the probe for TC and OTC, which provided the application possibility for the intelligent detection of the probe.

A confined carbon dot-based self-calibrated fluorescence probe for visible and high-sensitivity moisture readouts

2021 ◽  
Author(s):  
Zhong-Zheng Ding ◽  
Guang-Song Zheng ◽  
Qing Lou ◽  
Jiang-Fan Han ◽  
Meng-Yuan Wu ◽  
...  
Keyword(s):  
High Performance ◽  
Potential Role ◽  
Fluorescence Probe ◽  
Color Change ◽  
High Sensitivity ◽  
Confinement Effect ◽  
Detection Range ◽  
Fluorescence Color ◽  
Highly Sensitive ◽  
New Type

Abstract Excellent luminescent materials are essential for high-performance fluorescent nanosensors. Here, a new-type self-calibrated humidity sensor has been established through monitoring the fluorescent color change of carbon dots (CDs) confined in sodium hydroxide (CDs@NaOH). The CDs are prepared by a facile and rapid microwave assisted heating method using citric acid, urea, and NaOH as precursors. The confinement effect from NaOH has reduced the nonradiative transition and suppressed the aggregation-induced quenching of the CDs in solid. Compared with other sensors based on CD fluorescent visualization, the sensor has good linearity and wide humidity detection range from 6.9% to 95.4%. With the increased relative humidity, the fluorescence color of the sensor change from green to blue. The proposed sensing mechanism is due to the breaking and reforming of hydrogen bonds and proton transfer occurring at the CD-NaOH matrix interfaces. This finding suggests a potential role for the spatial confinement effect and may provide an avenue for developing highly sensitive humidity readouts.

scholarly journals A New Coumarin-Acridone Compound as a Fluorescence Probe for Fe3+ and Its Application in Living Cells and Zebrafish

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2115
Author(s):  
Jiayong Huang ◽  
Zhenshuo Yan ◽  
Peiling Qiu ◽  
Yufeng Mo ◽  
Qizhen Cao ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
High Resolution Mass Spectrometry ◽  
High Sensitivity ◽  
Cell Membrane Permeability ◽  
Buffer Solution ◽  
Intracellular Iron ◽  
Testing Metal ◽  
Imaging Results ◽  
Sensitivity And Selectivity ◽  
Low Cytotoxicity

A new coumarin-acridone fluorescent probe S was designed and synthesized, and the structure was confirmed with 1H/13C NMR spectrometry, single-crystal X-ray diffraction, and high-resolution mass spectrometry. This probe has high sensitivity and selectivity for Fe3+ over other testing metal ions at 420 or 436 nm in acetonitrile–MOPS (3-Morpholinopropanesulfonic Acid) buffer solution (20.0 μM, pH = 6.9, 8:2 (v/v)). Under physiological conditions, the probe displayed satisfying time stability with a detection limit of 1.77 µM. In addition, probe S was successfully used to detect intracellular iron changes through a fluorescence-off mode, and the imaging results of cells and zebrafish confirmed their low cytotoxicity and satisfactory cell membrane permeability, as well as their potential biological applications.

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

scholarly journals Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 667
Author(s):  
Pavel Rudenko ◽  
Yuriy Vatnikov ◽  
Nadezhda Sachivkina ◽  
Andrei Rudenko ◽  
Evgeny Kulikov ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
High Efficiency ◽  
Biological Marker ◽  
High Sensitivity ◽  
Antagonistic Activity ◽  
Adhesive Properties ◽  
Surgical Infection ◽  
Surgical Infections ◽  
Causative Agents ◽  
Inflammatory Processes

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum “Victoria” No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum “Victoria” No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum “Victoria” No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

A simple chemosensor for the dual-channel detection of cyanide in water with high selectivity and sensitivity

RSC Advances ◽  
2016 ◽  
Vol 6 (32) ◽  
pp. 27130-27135 ◽  
Author(s):  
Xiao-Bin Cheng ◽  
Hui Li ◽  
Feng Zheng ◽  
Qi Lin ◽  
Hong Yao ◽  
...  
Keyword(s):  
High Selectivity ◽  
Aqueous Media ◽  
High Sensitivity ◽  
Channel Detection ◽  
Dual Channel ◽  
Sensitivity And Selectivity

By a deprotonated mechanism, the simple chemosensorHYshowed high sensitivity and selectivity for cyanide in aqueous media.

A fluorescent colorimetric azo dye based chemosensor for detection of S2− in perfect aqueous solution and its application in real sample analysis and building a molecular logic gate

2018 ◽  
Vol 10 (20) ◽  
pp. 2317-2326 ◽  
Author(s):  
Amit Kumar Manna ◽  
Jahangir Mondal ◽  
Rukmani Chandra ◽  
Kalyani Rout ◽  
Goutam Kumar Patra
Keyword(s):  
Aqueous Solution ◽  
Schiff Base ◽  
Azo Dye ◽  
Color Change ◽  
Logic Gate ◽  
Real Sample ◽  
Sample Analysis ◽  
Real Sample Analysis ◽  
Molecular Logic Gate ◽  
Molecular Logic

A fluorescent-colorimetric azo dye based bis-Schiff base chemosensor, L, has been developed. L can selectively detect S2− through a color change from yellow to orange in perfect aqueous solution by deprotonation mechanism.

A Label-Free Fluorescent AND Logic Gate Aptasensor for Carbohydrate Antigen 15-3 Detection Based on Graphene Oxide

2021 ◽  
Vol 24 ◽  
Author(s):  
Wenxiao Hu ◽  
Yafei Dong ◽  
Luhui Wang ◽  
Yue Wang ◽  
Mengyao Qian ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Logic Gate ◽  
High Sensitivity ◽  
Carbohydrate Antigen ◽  
Fluorescence Signal ◽  
Label Free ◽  
Molecular Logic ◽  
Long Stem ◽  
Middle Ring ◽  
Fluorescent Aptasensor

Background: Molecular logic gate always used fluorescent dyes to realize fluorescence signal. The labeling of the fluorophore is relatively expensive, low yield and singly labeled impuritiesaffects the affinity between the target and the aptamer. Label-free fluorescent aptamer biosensor strategy has attracted widespread interest due to lower cost and simple. Objective: Herein, we have designed a AND logic gate fluorescent aptasensor for detecting carbohydrate antigen 15-3(CA15-3) based on label-free fluorescence signal output. Materials and Methods: A hairpin DNA probe consists of CA15-3 aptamer and partly anti-CA15-3 aptamer sequences as a long stem and G-rich sequences of the middle ring as a quadruplex-forming oligomer. G-rich sequences can fold into a quadruplex by K+, and then G-quadruplex interacts specifically with N-methylmesoporphyrin IX(NMM), leading to a dramatic increase in fluorescence of NMM. With CA15-3 and NMM as the two inputs, the fluorescence intensity of the NMM is the output signal. Lacking of CA15-3 or NMM, there is no significant fluorescence enhancing, and the output of the signal is “0”. The fluorescence signal was dramatically increasing and the output of the signal is “1” only when CA15-3 protein and NMM were added at the same time. Results: This biosensor strategy possessed selectivity, high sensitivity for detecting CA15-3 protein from 10 to 500 U mL-1 and the detection limit was 10 U mL-1, and also showed good reproducibility in spiked human serum. Conclusion: In summary, the proposed AND logic gate fluorescent aptasensor could specifically detect CA15-3.

Easy-to-Fabricate K2Pd(SO3)2-Dyed Polyester Fabric with Highly Selective and Fast Response to Carbon Monoxide

2021 ◽  
Vol 21 (8) ◽  
pp. 4400-4405
Author(s):  
Junyeop Lee ◽  
Nam Gon Do ◽  
Dong Hyuk Jeong ◽  
Sae-Wan Kim ◽  
Maeum Han ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Color Change ◽  
High Sensitivity ◽  
Cost Effective ◽  
Fast Response ◽  
Polyester Fabric ◽  
High Porosity ◽  
Sensing Material ◽  
Sensitivity And Selectivity ◽  
And Performance

Carbon monoxide (CO) is an odorless, colorless, tasteless, extremely flammable, and highly toxic gas. It is produced when there is insufficient oxygen supply during the combustion of carbon to produce carbon dioxide (CO2). CO is produced from operating engines, stoves, or furnaces. CO poisoning occurs when CO accumulates in the bloodstream and can result in severe tissue damage or even death. Many types of CO sensors have been reported, including electrochemical, semiconductor metal-oxide, catalytic combustion, thermal conductivity, and infrared absorption-type for the detection of CO. However, despite their excellent selectivity and sensitivity, issues such as complexity, power consumption, and calibration limit their applications. In this study, a fabricbased colorimetric CO sensor is proposed to address these issues. Potassium disulfitopalladate (II) (K2Pd(SO3)2) is dyed on a polyester fabric as a sensing material for selective CO detection. The sensing characteristics and performance are investigated using optical instruments such as RGB sensor and spectrometer. The sensor shows immediate color change when exposed to CO at a concentration that is even lower than 20 ppm before 2 min. The fast response time of the sensor is attributed to its high porosity to react with CO. This easy-to-fabricate and cost-effective sensor can detect and prevent the leakage of CO simultaneously with high sensitivity and selectivity toward CO.

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