scholarly journals Highly Specific Loop-Mediated Isothermal Amplification Using Graphene Oxide–Gold Nanoparticles Nanocomposite for Foot-and-Mouth Disease Virus Detection

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 264
Author(s):  
Jong-Won Kim ◽  
Kyoung-Woo Park ◽  
Myeongkun Kim ◽  
Kyung Kwan Lee ◽  
Chang-Soo Lee

Loop-mediated isothermal amplification (LAMP) is a molecular diagnosis technology with the advantages of rapid results, isothermal reaction conditions, and high sensitivity. However, this diagnostic system often produces false positive results due to a high rate of non-specific reactions caused by formation of hairpin structures, self-dimers, and mismatched hybridization. The non-specific signals can be due to primers used in the methods because the utilization of multiple LAMP primers increases the possibility of self-annealing of primers or mismatches between primers and templates. In this study, we report a nanomaterial-assisted LAMP method that uses a graphene oxide–gold nanoparticles (AuNPs@GO) nanocomposite to enable the detection of foot-and-mouth disease virus (FMDV) with high sensitivity and specificity. Foot-and-mouth disease (FMD) is a highly contagious and deadly disease in cloven-hoofed animals; hence, a rapid, sensitive, and specific detection method is necessary. The proposed approach exhibited high sensitivity and successful reduction of non-specific signals compared to the traditionally established LAMP assays. Additionally, a mechanism study revealed that these results arose from the adsorption of single-stranded DNA on AuNPs@GO nanocomposite. Thus, AuNPs@GO nanocomposite is demonstrated to be a promising additive in the LAMP system to achieve highly sensitive and specific detection of diverse diseases, including FMD.

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1921
Author(s):  
Jong-Won Kim ◽  
Myeongkun Kim ◽  
Kyung Kwan Lee ◽  
Kwang Hyo Chung ◽  
Chang-Soo Lee

The polymerase chain reaction (PCR) has become a powerful molecular diagnostic technique over the past few decades, but remains somewhat impaired due to low specificity, poor sensitivity, and false positive results. Metal and carbon nanomaterials, quantum dots, and metal oxides, can improve the quality and productivity of PCR assays. Here, we describe the ability of PCR assisted with nanomaterials (nano-PCR) comprising a nanocomposite of graphene oxide (GO) and gold nanoparticles (AuNPs) for sensitive detection of the foot-and-mouth disease virus (FMDV). Graphene oxide and AuNPs have been widely applied as biomedical materials for diagnosis, therapy, and drug delivery due to their unique chemical and physical properties. Foot-and-mouth disease (FMD) is highly contagious and fatal for cloven-hoofed animals including pigs, and it can thus seriously damage the swine industry. Therefore, a highly sensitive, specific, and practical method is needed to detect FMDV. The detection limit of real-time PCR improved by ~1000 fold when assisted by GO-AuNPs. We also designed a system of detecting serotypes in a single assay based on melting temperatures. Our sensitive and specific nano-PCR system can be applied to diagnose early FMDV infection, and thus may prove to be useful for clinical and biomedical applications.


Gold Bulletin ◽  
2015 ◽  
Vol 48 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Lev A. Dykman ◽  
Sergey A. Staroverov ◽  
Pavel V. Mezhenny ◽  
Alexander S. Fomin ◽  
Sergey V. Kozlov ◽  
...  

2016 ◽  
Vol 15 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Solmaz Rafiei ◽  
Seyedeh Elham Rezatofighi ◽  
Mohammad Roayaei Ardakani ◽  
Saadat Rastegarzadeh

VirusDisease ◽  
2018 ◽  
Vol 29 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Beenu Jain ◽  
Upendra Lambe ◽  
Anuj Tewari ◽  
Surender Kumar Kadian ◽  
Minakshi Prasad

1979 ◽  
Vol 83 (1) ◽  
pp. 127-134 ◽  
Author(s):  
E. M. E. Abu Elzein ◽  
J. R. Crowther

SUMMARYA solid-phase micro-enzyme-labelled immunosorbent assay (ELISA) using guinea pig antiserum against purified (140S) inactivated foot-and-mouth disease (FMD) virus has been usedin a sandwich technique to specifically measure 140S virus in the presence of 12S material.


2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Mervat E. Hamdy ◽  
Michele Del Carlo ◽  
Hussein A. Hussein ◽  
Taher A. Salah ◽  
Ayman H. El-Deeb ◽  
...  

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