scholarly journals Effects of Soy Isoflavones, Resistant Starch and Antibiotics on Polycystic Ovary Syndrome (PCOS)-Like Features in Letrozole-Treated Rats

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3759
Author(s):  
Geethika S. G. Liyanage ◽  
Ryo Inoue ◽  
Mina Fujitani ◽  
Tomoko Ishijima ◽  
Taisei Shibutani ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Butyric Acid ◽  
Resistant Starch ◽  
Polycystic Ovary ◽  
Soy Isoflavones ◽  
Menstrual Irregularity ◽  
Dietary Interventions ◽  
Ovary Syndrome ◽  
Soy Isoflavone

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in reproductive-aged women. Recently, various dietary interventions have been used extensively as a novel therapy against PCOS. In the present study, we show that soy isoflavone metabolites and resistant starch, together with gut microbiota modulations, were successful in decreasing the severity of PCOS-like reproductive features while increasing the expression of gut barrier markers and butyric acid in the gut. In the letrozole-induced PCOS model rats, the intake of both 0.05% soy isoflavones and 11% resistant starch, even with letrozole treatment, reduced the severity of menstrual irregularity and polycystic ovaries with a high concentration of soy isoflavones and equol in plasma. Antibiotic cocktail treatment suppressed soy isoflavone metabolism in the gut and showed no considerable effects on reducing the PCOS-like symptoms. The mRNA expression level of occludin significantly increased with soy isoflavone and resistant starch combined treatment. Bacterial genera such as Blautia, Dorea and Clostridium were positively correlated with menstrual irregularity under resistant starch intake. Moreover, the concentration of butyric acid was elevated by resistant starch intake. In conclusion, we propose that both dietary interventions and gut microbiota modulations could be effectively used in reducing the severity of PCOS reproductive features.

Polycystic ovary syndrome and gut microbiota

2021 ◽  
Vol 3_2021 ◽  
pp. 36-43
Author(s):  
Chernukha G.E. Chernukha ◽  
Miroshina E.D. Miroshina ◽  
Priputnevich T.V. Priputnevich ◽  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Polycystic Ovary ◽  
Ovary Syndrome

scholarly journals Inulin and metformin ameliorate polycystic ovary syndrome via anti-inflammation and modulating gut microbiota in mice

2019 ◽  
Vol 66 (10) ◽  
pp. 859-870 ◽  
Author(s):  
Jing Xue ◽  
Xiaorong Li ◽  
Ping Liu ◽  
Ke Li ◽  
Liping Sha ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Anti Inflammation

Circulating gut microbiota metabolite trimethylamine N‐oxide and oral contraceptive use in polycystic ovary syndrome

2019 ◽  
Vol 91 (6) ◽  
pp. 810-815 ◽  
Author(s):  
Nesrin Damla Eyupoglu ◽  
Ezgi Caliskan Guzelce ◽  
Aylin Acikgoz ◽  
Esra Uyanik ◽  
Bodil Bjørndal ◽  
...  
Keyword(s):  
Oral Contraceptive ◽  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Contraceptive Use ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Oral Contraceptive Use

scholarly journals Soy isoflavones alleviate polycystic ovary syndrome in rats by regulating NF- κB signaling pathway

Bioengineered ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 7215-7223
Author(s):  
Xiaohong Ma ◽  
Xiaorong Li ◽  
LiLi Ma ◽  
Yan Chen ◽  
Shengyu He
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Signaling Pathway ◽  
Polycystic Ovary ◽  
Soy Isoflavones ◽  
Ovary Syndrome

scholarly journals Shaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhuang-peng Chang ◽  
Gui-feng Deng ◽  
Yun-yun Shao ◽  
Ding Xu ◽  
Yi-nan Zhao ◽  
...  
Keyword(s):  
Body Weight ◽  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Signaling Pathway ◽  
Inflammatory Cytokines ◽  
Polycystic Ovary ◽  
Therapeutic Effects ◽  
Ovary Syndrome ◽  
Protein Expressions ◽  
Pro Inflammatory Cytokines

Background: Emerging evidence suggests that gut microbiota plays a vital role in the occurrence of multiple endocrine disorders including polycystic ovary syndrome (PCOS). Shaoyao-Gancao Decoction (SGD), a classical Chinese prescription, has been widely used in the treatment of PCOS for decades. In previous studies, we found that SGD treatment could effectively reduce ovarian inflammation in PCOS rats. However, whether the anti-inflammation effect of SGD involves the regulation of the gut microbiota remains elusive.Methods: Letrozole-induced PCOS rat models were established, and the therapeutic effects of SGD were evaluated. Specifically, body weight, serum hormone concentrations, estrus phase and ovary histopathology were assessed. Then the structure of gut microbiota was determined by 16s rRNA sequencing. Additionally, the serum levels of pro-inflammatory cytokines and LPS were measured by ELISA kits. The key gene and protein expressions of TLR4/NF-κB signaling pathway were detected by quantitative real-time PCR and western blot.Results: SGD could effectively reduce body weight, regulate estrous cycles and ameliorate hyperandrogenism in PCOS rats. In addition, SGD treatment decreased releases of pro-inflammatory cytokines, enhanced the expressions of tight junction (occludin and claudin1), and then prevented a translocation of LPS into bloodstream. SGD could significantly reduce the ratio of Firmicutes to Bacteroidetes, decrease the abundance of LPS-producing pathogens Proteobateria and enrich the abundance of Butyricicoccus, Coprococcus, Akkermansia Blautia and Bacteroides in PCOS rats. Furthermore, SGD blunted the key gene and protein expressions of TLR4/NF-κB signaling pathway both in vivo and in LPS-induced RAW264.7 cells.Conclusion: SGD administration could ameliorate the inflammatory response in PCOS rats by remodeling gut microbiome structure, protecting gut barrier, and suppressing TLR4/NF-κB signaling pathway.

scholarly journals 16S rDNA Full-Length Assembly Sequencing Technology Analysis of Intestinal Microbiome in Polycystic Ovary Syndrome

2021 ◽  
Vol 11 ◽  
Author(s):  
Sitong Dong ◽  
Jiao jiao ◽  
Shuangshuo Jia ◽  
Gaoyu Li ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
16S Rdna ◽  
Polycystic Ovary ◽  
Full Length ◽  
Healthy Controls ◽  
Sequencing Technology ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Pcos Group

ObjectiveTo study the characteristics and relationship of the gut microbiota in patients with polycystic ovary syndrome (PCOS).MethodWe recruited 45 patients with PCOS and 37 healthy women from the Reproductive Department of Shengjing Hospital. We recorded their clinical indexes, and sequenced their fecal samples by 16S rDNA full-length assembly sequencing technology (16S-FAST).ResultWe found decreased α diversity and different abundances of a series of microbial species in patients with PCOS compared to healthy controls. We found LH and AMH were significantly increased in PCOS with Prevotella enterotype when compared to control women with Prevotella enterotype, while glucose and lipid metabolism level remained no significant difference, and situations were opposite in PCOS and control women with Bacteroides enterotype. Ruminococcus gnavus, Prevotella stercorea, Dialister succinatiphilus and Bacteroides fragilis were more abundant while Christensenellaceae spp. were less abundant in the PCOS group. P. stercorea was significantly more prevalent in PCOS-not insulin resistance (NIR) compared to control-NIR and PCOS-not overweight (NOW) patient groups compared to control-NOW groups. Kyoto Encyclopedia Genes and Genomes reflecting pathways related to lipopolysaccharide biosynthesis were more abundant in the PCOS group.ConclusionOur study found gut microbiota that had different abundance in patients with PCOS compared to healthy controls. An intimate relationship was shown between the gut microbiota and pathological changes in PCOS. We suggest the gut microbiota should be taken into consideration in the treatment of symptoms of PCOS via drugs and diet.

scholarly journals Effects of Sleeve Gastrectomy on Fecal Gut Microbiota and Short-Chain Fatty Acid Content in a Rat Model of Polycystic Ovary Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Wei Lin ◽  
Lingying Wen ◽  
Junping Wen ◽  
Guangda Xiang
Keyword(s):  
Sleeve Gastrectomy ◽  
Fatty Acid ◽  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Rat Model ◽  
Short Chain Fatty Acid ◽  
Polycystic Ovary ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Ovary Syndrome

PurposeSleeve gastrectomy (SG) is a surgical intervention for polycystic ovary syndrome (PCOS), especially for patients with obesity. Here, we explored the effects of SG on the gut microbiota of rats with PCOS and investigated the association between the intestinal flora and efficacy of SG in PCOS.MethodsDehydroepiandrosterone (DHEA) injection was administered alone and in combination with a high-fat diet to induce PCOS in rats. SG was performed in rats with PCOS, and the effects of SG on the fecal and gut microbiota and the short-chain fatty acid (SCFA) content were observed. Furthermore, the association among gut microbiota, SCFA content and hyperandrogenism or other hallmarks of PCOS was evaluated.ResultsThe abundance of Firmicutes reduced and that of Bacteroidetes increased in response to SG in the DHEA-induced PCOS rat model. At the genus level, the abundances of Bacteroides and Blautia increased and those of Ruminococcus, Clostridium, and Alistipes reduced distinctly in the PCOS-SG groups. Moreover, the levels of fecal SCFAs, especially butyric acid, reduced after SG. SG significantly ameliorated PCOS-related symptoms such as hyperandrogenism, disrupted ovary function, and impaired glucose tolerance. Bacteroides and Blautia exhibited a negative correlation and Ruminococcus, Clostridium, and Alistipes exhibited a positive correlation with the levels of fecal SCFAs, luteinizing hormone, testosterone, and inflammatory factors.ConclusionsThe amelioration of PCOS-related reproductive and metabolic disorders following SG was associated with the regulation of microbial taxa and SCFA content. Our findings provide a novel perspective on the microbial mechanisms in PCOS after SG.

Gut microbiota dysbiosis in polycystic ovary syndrome: association with obesity — a preliminary report

2020 ◽  
Vol 98 (11) ◽  
pp. 803-809 ◽  
Author(s):  
Yuanjiao Liang ◽  
Qi Ming ◽  
Jinlan Liang ◽  
Yan Zhang ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Gut Microbiota ◽  
Polycystic Ovary ◽  
Alpha Diversity ◽  
Shannon Index ◽  
Normal Weight ◽  
Microbiota Composition ◽  
Ovary Syndrome ◽  
Gut Microbiota Dysbiosis ◽  
Gut Microbiota Composition

The objective was to explore if and how the microbiota changed in polycystic ovary syndrome (PCOS) women compared with healthy women. Eight obese PCOS (PO group), 10 nonobese PCOS (PN group), and nine healthy normal weight women (control) (C group) were enrolled. Insulin (INS), testosterone (T), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2), and dehydroepiandrosterone (DHEA) were detected with radioimmunoassay. Antimullerian hormone (AMH), fasting glucose, and hemoglobin A1c (HbA1c) were determined by a chemiluminescence immunoassay, glucose oxidase method, and HPLC, respectively. Gut microbiota composition was evaluated by PCR. Alpha diversity was assessed using Chao1 and the Shannon index. PCOS women showed significantly higher T, LH, and LH/FSH and lower FSH levels than the C group (p < 0.05). The AMH level was significantly higher in the PO than in the PN group (p < 0.05). The PO group presented a significantly higher fasting INS level and HMOA-IR scores than the other groups, lower observed SVs and alpha diversity than the C group, higher beta diversity than the PN group (p < 0.05), and decreased abundances of genera (mainly butyrate producers). Regression analysis showed that decreased abundances of several genera were correlated with higher circulating T and impaired glucose metabolism. PCOS is associated with changes in the gut microbiota composition. Obesity has a driving role in the development of dysbiotic gut microbiota in PCOS.

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