scholarly journals Application of TaqMan Real-Time PCR for Detecting ‘Candidatus Arsenophonus Phytopathogenicus’ Infection in Sugar Beet

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1466
Author(s):  
Christina Zübert ◽  
Michael Kube
Keyword(s):  
Heat Shock ◽  
Sugar Beet ◽  
Heat Shock Protein ◽  
Real Time Pcr ◽  
Biomass Yield ◽  
Sugar Content ◽  
End Point ◽  
Genes Encoding ◽  
Synthetic Oligonucleotides ◽  
Taqman Qpcr

The γ-proteobacterium ‘Candidatus Arsenophonus phytopathogenicus’ is assigned as the major pathogen of “Syndrome des basses richesses”, a sugar beet disease characterised by a reduction in the sugar content of taproots and biomass yield. Despite the economic impact of this bacteriosis, diagnostics for this important pathogen currently rely on end-point PCR detection. Herein, we introduce a TaqMan qPCR for diagnostics of the agent targeting genes encoding a heat shock protein of the Hsp20 family and mannose-6-phosphate isomerase. Quantitation with synthetic oligonucleotides as standard showed that the developed TaqMan qPCR assays enable the detection of up to 100 target copies. A comparison between the TaqMan qPCR and end-point PCR for ‘Ca. A. phytopathogenicus’ detection was carried out on 78 sugar beet samples from different locations in southern Germany. The newly developed assays enable the fast, reliable and sensitive detection of ‘Ca. A. phytopathogenicus’ in sugar beet.

scholarly journals Sequence and organization of genes encoding the human 27 kDa heat shock protein

1986 ◽  
Vol 14 (20) ◽  
pp. 8229-8229 ◽  
Author(s):  
Eileen Hickey ◽  
Susan E. Brandon ◽  
Robert Potter ◽  
Gary Stein ◽  
Janet Stein ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Genes Encoding

Real-Time PCR Detection of Staphylococcus aureus in Milk and Meat Using New Primers Designed from the Heat Shock Protein Gene htrA Sequence

2007 ◽  
Vol 70 (12) ◽  
pp. 2855-2859 ◽  
Author(s):  
YU-CHENG CHIANG ◽  
CHIH-MING FAN ◽  
WAN-WEN LIAO ◽  
CHIEN-KU LIN ◽  
HAU-YANG TSEN
Keyword(s):  
Staphylococcus Aureus ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Real Time ◽  
Real Time Pcr ◽  
Protein Gene ◽  
Pcr Detection ◽  
Bacterial Strains ◽  
Heat Shock Protein Gene ◽  
Positive Results

Staphylococcus aureus may cause foodborne disease outbreaks and staphylococcal infections and is one of the major causes of mastitis. Rapid and reliable methods for detection of this microorganism in milk and other foods are needed. In this study, we designed a primer set from the sequence of the heat shock protein gene htrA, a gene coding for high-temperature-requirement A (HtrA) protein, and used it for real-time PCR detection of S. aureus isolates: 16 reference strains and 40 strains isolated from food-poisoning cases. All strains tested generated positive results. Bacterial strains other than S. aureus, including strains of other Staphylococcus species, did not produce positive results. When this primer set was used for the real-time PCR detection of S. aureus in milk and meat samples without the preenrichment step, samples with target cell numbers greater than 103 CFU/ml or CFU/g could be detected, indicating the potential quantitative ability of this real-time PCR assay. With a 10-h preenrichment step, however, a detection limit of 1 CFU/ml or CFU/g could be obtained.

scholarly journals Sequence and organization of genes encoding the human 27 kDa heat shock protein

1986 ◽  
Vol 14 (10) ◽  
pp. 4127-4145 ◽  
Author(s):  
Eileen Hickey ◽  
Susan E. Brandon ◽  
Robert potter ◽  
Gary Stein ◽  
Janet Stein ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Genes Encoding
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