Angiopoietin-Like Protein 4 and Insulin-Like Growth Factor-1 Expression in Invasive Breast Carcinoma in Young Women

Biomarker identification is imperative for invasive breast carcinoma, which is more aggressive and associated with higher mortality and worse prognosis in younger patients (<45 years) than in older patients (>50 years). The current study aimed to investigate angiopoietin-like protein 4 (ANGPTL4) and insulin-like growth factor-1 (IGF-1) protein expression in breast tissue from young patients with breast carcinoma. Immunohistochemical staining was applied in formalin-fixed, paraffin-embedded samples of breast carcinoma tissue from young patients aged <45 years at the time of diagnosis. Both proteins were expressed in the majority of cases. The highest frequency of positive ANGPTL4 and IGF-1 expression was observed in the luminal A subtype, whereas the HER2-overexpression subtype exhibited the lowest expression frequency for both proteins. There was no significant association between ANGPTL4 (p = 0.897) and IGF-1 (p = 0.091) expression and molecular subtypes of breast carcinoma. The histological grade was a significant predictor of ANGPTL4 expression (grade 1 vs. grade 3, adjusted odds ratio = 12.39, p = 0.040). Therefore, ANGPTL-4 and IGF-1 expressions are common in young breast carcinoma tissue. There is a potential use of them as biomarkers in breast carcinoma.

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Clinicopathological significance of WT1 expression in invasive breast carcinoma with >90% mucinous component

Journal of Clinical Pathology ◽

10.1136/jclinpath-2021-207464 ◽

2021 ◽

pp. jclinpath-2021-207464

Author(s):

Xiaoli Xu ◽

Rui Bi ◽

Ruohong Shui ◽

Baohua Yu ◽

Yufan Cheng ◽

...

Keyword(s):

Breast Carcinoma ◽

Growth Pattern ◽

Histological Grade ◽

Invasive Breast Carcinoma ◽

Nuclear Grade ◽

Proliferation Index ◽

Her2 Overexpression ◽

Ki 67 ◽

Mucinous Component ◽

Clinicopathological Significance

AimsThis study was aimed to investigate the clinicopathological significance of immunohistochemical (IHC) Wilm’s tumour 1 (WT1) expression in invasive breast carcinoma with >90% mucinous components.MethodsOne hundred specimens of invasive breast carcinoma with >90% mucinous component were collected. All H&E-stained slides were reviewed, and the clinicopathological data, including sex, age, tumour size, nuclear grade, histological grade, growth pattern and lymph node (LN) status, were collected. IHC staining of WT1, oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 was performed. Fluorescence in situ hybridisation was used to verify the amplification of the HER2 gene. The relationship between WT1 expression and clinicopathological features was analysed statistically.ResultsWT1 expression was detected in 67% (67/100) of invasive breast carcinoma with >90% mucinous components. WT1 expression was significantly associated with low-to-intermediate nuclear grade/histological grade, ER and PR positivity, HER2 negativity, Ki-67 proliferation index <30% and noLN metastasis (all p<0.001). Micropapillary architecture was observed in 80% of cases. WT1 expression was not significantly correlated with different percentage of micropapillary components (p=0.422). None of the histological grade 3 tumours, tumours with HER2 overexpression/amplification and triple-negative specimens showed WT1 expression.ConclusionsWT1 expression was significantly related with low-intermediate nuclear/histological grade, ER positivity, HER2 negativity, a lower Ki-67 proliferation index and no LN metastasis in invasive breast carcinoma with >90% mucinous component. The micropapillary growth pattern in this type of tumour did not show a specific relationship with WT1 expression.

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The insulin-like growth factor receptor I status in luminal A breast carcinoma.

10.1158/0008-5472.sabcs-4034 ◽

2009 ◽

Author(s):

A Ben Hamida ◽

F Sircoulomb ◽

I Beckhouche ◽

M Chaffanet ◽

F Bertucci ◽

...

Keyword(s):

Growth Factor ◽

Breast Carcinoma ◽

Growth Factor Receptor ◽

Insulin Like Growth Factor ◽

Luminal A

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Insulin-like growth factor binding protein-3 increases intracellular calcium concentrations in MCF-7 breast carcinoma cells

FEBS Letters ◽

10.1016/s0014-5793(02)03250-7 ◽

2002 ◽

Vol 527 (1-3) ◽

pp. 293-297 ◽

Cited By ~ 17

Author(s):

Jean-Marc Ricort ◽

Alain Lombet ◽

Claudine Lassarre ◽

Michel Binoux

Keyword(s):

Growth Factor ◽

Breast Carcinoma ◽

Intracellular Calcium ◽

Binding Protein ◽

Carcinoma Cells ◽

Insulin Like Growth Factor ◽

Factor Binding ◽

Breast Carcinoma Cells ◽

Mcf 7

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Insulin-like growth factor binding protein-3 stimulates phosphatidylinositol 3-kinase in MCF-7 breast carcinoma cells

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2004.01.001 ◽

2004 ◽

Vol 314 (4) ◽

pp. 1044-1049 ◽

Cited By ~ 10

Author(s):

Jean-Marc Ricort ◽

Michel Binoux

Keyword(s):

Growth Factor ◽

Breast Carcinoma ◽

Binding Protein ◽

Carcinoma Cells ◽

Insulin Like Growth Factor ◽

Phosphatidylinositol 3 Kinase ◽

Factor Binding ◽

Breast Carcinoma Cells ◽

Mcf 7 ◽

Phosphatidylinositol 3

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CLINICAL AND HISTOPATHOLOGICAL REVIEW OF INVASIVE BREAST CARCINOMA IN A TERITARY CARE CENTRE.

10.36106/5735570 ◽

2021 ◽

pp. 18-21

Author(s):

A. Gomathy ◽

Muruganantham Arunagirinathan ◽

I. Nithya

Keyword(s):

Breast Cancer ◽

Breast Carcinoma ◽

Cancer Survival ◽

Histological Grade ◽

Lymph Node Involvement ◽

Left Breast ◽

Invasive Breast Carcinoma ◽

Indian Women ◽

Node Involvement ◽

Grade Ii

BACKGROUND: Breast cancer accounts for 14% of all cancers in Indian women, that can occur at any age. Cancer survival becomes more difcult in higher stages of tumour, hence in order to improve the survival of affected persons, early diagnosis of breast cancer is critical. METHODS: Retrospective study of 48 mastectomy specimens with relevant clinical details and respective H&E stained slides were reviewed. CONCLUSION: This review showed that occurrence of Invasive Breast Carcinoma(IBC) peaks in the age group of 41-50years (35.4% ) with right and left breast being affected equally in the ratio of R:L – 1 : 1. Most of the IBC (91.6%) were of No Special Type (NST), with 75% of tumours were of Histological Grade II. 58.3% of tumours were of tumour stage T along with lymph node involvement in equal number of cases.

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Immunohistochemical and Clinicopathologic Characteristics of Invasive Ductal Carcinoma of Breast With Micropapillary Carcinoma Component

Archives of Pathology & Laboratory Medicine ◽

10.5858/2005-129-1277-iaccoi ◽

2005 ◽

Vol 129 (10) ◽

pp. 1277-1282 ◽

Cited By ~ 4

Author(s):

Mi-Jung Kim ◽

Gyungyub Gong ◽

Hee Jae Joo ◽

Se-Hyun Ahn ◽

Jae Y. Ro

Keyword(s):

Estrogen Receptor ◽

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Breast Carcinoma ◽

Epidermal Growth Factor ◽

Growth Factor Receptor ◽

Invasive Breast Carcinoma ◽

Nodal Metastases ◽

Micropapillary Carcinoma ◽

Epidermal Growth

Abstract Context.—A micropapillary carcinoma (MC) component is generally considered to behave aggressively. Although several reports have described the prognostic significance of MC in breast carcinomas, immunohistochemical findings of MC, especially as compared to non-MC, are rarely described. Objective.—We compared clinicopathologic and immunohistochemical findings between 38 cases of invasive breast carcinoma with an MC component (IMC) and 217 cases of invasive breast carcinoma without an MC component (NIMC). Design.—We constructed a tissue microarray from 38 cases of IMC and performed immunohistochemical stainings for cytokeratin (CK) 7, CK20, estrogen receptor, progesterone receptor, p53, c-Erb-B2, CD34, CK5, epidermal growth factor receptor, and c-Kit in both MC and non-MC components. Results.—Cases with IMC were associated with greater tumor size, more frequent lymphovascular invasion, nodal metastases, greater mean numbers of positive lymph nodes, and higher stage than those with NIMC, but were not associated with poorer survival rates. On immunohistochemistry, only p53 reactivity was statistically different between MC and non-MC components in IMC cases. Estrogen receptor positivity tended to be lower in MC than non-MC, but the difference was not significant. Most of the MCs and non-MCs in IMC cases were positive for CK7, but none of them were positive for CK20, CK5, epidermal growth factor receptor, or c-Kit. Conclusions.—Based on the frequent nodal metastases and association with higher stage found in IMC as compared with NIMC cases, as well as higher p53 positivity and lower frequency of estrogen receptor expression, MC could be considered an aggressive histologic type of breast carcinoma. In both MC and non-MC components in IMC cases, no basallike immunostaining pattern was detected.

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