Effect of Neuropeptide S Administration on Ultrasonic Vocalizations and Behaviour in Rats with Low vs. High Exploratory Activity

Neuropeptide S (NPS) is a peptide neurotransmitter that in animal studies promotes wakefulness and arousal with simultaneous anxiety reduction, in some inconsistency with results in humans. We examined the effect of NPS on rat ultrasonic vocalizations (USV) as an index of affective state and on behaviour in novel environments in rats with persistent inter-individual differences in exploratory activity. Adult male Wistar rats were categorised as of high (HE) or low (LE) exploratory activity and NPS was administered intracerebroventricularly (i.c.v.) at a dose of 1.0 nmol/5 µL, after which USVs were recorded in the home-cage and a novel standard housing cage, and behaviour evaluated in exploration/anxiety tests. NPS induced a massive production of long and short 22 kHz USVs in the home cage that continued later in the novel environment; no effect on 50 kHz USVs were found. In LE-rats, the long 22 kHz calls were emitted at lower frequencies and were louder. The effects of NPS on behaviour appeared novelty- and test-dependent. NPS had an anxiolytic-like effect in LE-rats only in the elevated zero-maze, whereas in HE-rats, locomotor activity in the zero-maze and in a novel standard cage was increased. Thus NPS appears as a psychostimulant peptide but with a complex effect on dimensions of affect.

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Effect of tianeptine on neuroendocrine, enzyme and behavioral responses to restraint stress in male rats

European Psychiatry ◽

10.1017/s0924933800005411 ◽

1993 ◽

Vol 8 (S2) ◽

pp. 67s-73s ◽

Cited By ~ 1

Author(s):

R Fontanges ◽

J Mimouni ◽

X de Grieve ◽

J Picard ◽

M Pugeat ◽

...

Keyword(s):

Open Field ◽

Restraint Stress ◽

Wistar Rats ◽

Chronic Treatment ◽

Single Injection ◽

Behavioral Responses ◽

Chronic Administration ◽

Exploratory Activity ◽

Male Rats ◽

The Novel

SummaryThe effects of the novel antidepressant tianeptine, after acute or chronic administration, were compared in normal and restraint-stressed (30 min or 2 h) Wistar rats. Tianeptine, at the dose of 10 mg/kg, did not exert any effect in non-stressed rats. However, in animals restrained for 30 min, tianeptine reduced the increase of circulating ACTH and β-endorphin levels without modification of corticosterone. Moreover, it antagonized the deficit of vertical exploratory activity in an open field. In rats restrained for 2 hours, a single injection of tianeptine suppressed the stress-induced increase of TAT hepatic activity and moderately attenuated the deficit of activity in the open field. This effect was less marked and not statistically significant after chronic treatment.

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The Effect of Swimming on Anxiety-Like Behaviors and Corticosterone in Stressed and Unstressed Rats

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17186675 ◽

2020 ◽

Vol 17 (18) ◽

pp. 6675

Author(s):

Mohammad Amin Safari ◽

Maryam Koushkie Jahromi ◽

Rasoul Rezaei ◽

Hadi Aligholi ◽

Serge Brand

Keyword(s):

Wistar Rats ◽

Open Field Test ◽

Recovery Period ◽

Chronic Mild Stress ◽

Anxiety Reduction ◽

Future Research ◽

Mild Stress ◽

Swimming Training ◽

Male Wistar Rats ◽

Recovery Group

This study assessed the effect of swimming training on anxiety-like behaviors and corticosterone. Thirty adult male Wistar rats were randomly assigned to five study conditions: swimming training (ST); exposure to chronic mild stress (CS); exposure to chronic mild stress followed by swimming training (CS + ST); exposure to chronic mild stress followed by a recovery period (CS + recovery); control. The exercise training consisted of 60 min of swimming exercise per day, for five days a week, and four consecutive weeks. A chronic mild stress program (CMS) was applied for a period of four weeks. Anxiety-like behaviors were measured by open field test (OFT). The number of excrements and blood corticosterone were used as physiological parameters of anxiety. To assess corticosterone, blood samples were taken 48 h after the last session of experiments. Compared to other study conditions, the lowest anxiety-like behaviors and corticosterone concentrations were observed in the ST condition in unstressed rats. In stressed rats, as in the ST + CS group, swimming training probably reduced some anxiety behaviors, but the results showed increased corticosterone compared to control and CS + Recovery. Anxiety parameters and corticosterone concentrations were greatest in the CS condition. In the ST group, anxiety parameters were less than for the ST + CS group. In the CS + Recovery group, anxiety parameters were less than for the CS group. In summary, self-paced swimming training could attenuate some anxiety parameters in both stressed and non-stressed rats. The effect of swimming training in unstressed rats was more prominent than in stressed rats. In stressed rats, a period of recovery was more effective than swimming training in reducing corticosterone. Mechanisms of anxiety reduction other than cortisol should be investigated in future research.

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Response to novelty in rats tested in isolation and in pairs: focus on exploration and play

Polish Psychological Bulletin ◽

10.2478/v10059-008-0012-9 ◽

2008 ◽

Vol 39 (2) ◽

pp. 81-88 ◽

Cited By ~ 2

Author(s):

Łukasz Tanaś ◽

Rafał Stryjek

Keyword(s):

Wistar Rats ◽

Social Play ◽

Experimental Manipulation ◽

Exploratory Activity ◽

Experimental Chamber ◽

The Novel ◽

Novel Object ◽

Social Exploration ◽

Familiar Environment ◽

Play Interaction

Response to novelty in rats tested in isolation and in pairs: focus on exploration and play The main goal of the study was to compare investigatory responses towards novelty in 20 Wistar rats divided into two experimental groups (solitary exploration vs. exploration in pairs). Additionally, relationship between novelty and social play/interaction was analyzed in the dyad group. Procedure involved placing animals in an experimental chamber during fifteen, six minute trials on successive days of the study. On the eleventh session a new object was introduced. The results are summarized within several behavioral categories. Investigatory responses of rats in dyad to novel object in familiar environment were not quantitatively different, than those of isolated animals. The animals from both groups responded to the novel object by focusing their exploratory activity on the source of new stimulation. Amount of social play and social exploration was influenced by the experimental manipulation with important sex differences present.

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Screen for genes in periaqueductal grey of male Wistar rats related to reduced exploratory activity in the elevated plus-maze

Behavioural Brain Research ◽

10.1016/j.bbr.2007.05.017 ◽

2007 ◽

Vol 183 (1) ◽

pp. 8-17 ◽

Cited By ~ 2

Author(s):

A NELOVKOV ◽

S SUTT ◽

S RAUD ◽

E VASAR ◽

S KOKS

Keyword(s):

Wistar Rats ◽

Elevated Plus Maze ◽

Exploratory Activity ◽

Periaqueductal Grey ◽

Plus Maze ◽

Male Wistar Rats

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Female and Male Wistar Rats (Rattus norvegicus) Discriminate Diets According to Energetic Quantity

International Journal of Comparative Psychology ◽

10.46867/ijcp.2020.33.00.04 ◽

2020 ◽

Vol 33 ◽

Author(s):

Vinícius Warisaia ◽

Vítor Pansarim ◽

Davi C. Aragon ◽

Fernando S. Zucoloto ◽

Andréia Schmidt

Keyword(s):

Rattus Norvegicus ◽

Food Choice ◽

Wistar Rats ◽

Home Cage ◽

Response Cost ◽

Significant Preference ◽

Male Wistar Rats ◽

Flavor Test

The food choice of animals is influenced by several factors including the quantity and nutrients available. It is not known, however, whether faced with alternatives that present the same amount of food, with similar flavor and obtained with the same response cost, rats would discriminate between diets with different energetic quantities. The aim was to verify whether female and male Wistar rats (Rattus norvegicus) discriminate between three types of food that differ in their energetic content (whether or not they prefer one) and whether the flavor could affect the choice between two diets with equal energetic quantities. Twelve Wistar rats (six of each sex) underwent tests of choice between pairs of diets of different energetic values. After the tests, the animals had at their disposal, in the home cage, two diets with the same energetic content, which differed in flavor (one contained sucrose) - Flavor test. The consumption of each diet was measured for five consecutive days. All the subjects demonstrated a preference for the more energetic alternative, regardless of the combination of diets presented. In the Flavor test the animals did not show significant preference for any diet, i.e., the consumption of both the S and N diets were statistically equal for all subjects. It was concluded that the animals, regardless of sex, discriminated between the diets with different energetic values and that the flavor did not seem to be a determinant variable in the food choice.

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P.0238 Effect of neuropeptide s on ultrasonic vocalizations in rats: individual differences by trait-wise low vs. high exploratory activity

European Neuropsychopharmacology ◽

10.1016/j.euroneuro.2021.10.228 ◽

2021 ◽

Vol 53 ◽

pp. S172-S173

Author(s):

J. Harro ◽

K. Kõiv ◽

T. Uusen ◽

D. Matrov

Keyword(s):

Individual Differences ◽

Exploratory Activity ◽

Ultrasonic Vocalizations ◽

Neuropeptide S

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Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014333x ◽

1986 ◽

Vol 44 ◽

pp. 342-343

Author(s):

I. Stachura ◽

M. Pardo ◽

J. Costello ◽

D.M. Landwehr

Keyword(s):

Wistar Rats ◽

Renal Damage ◽

Standard Diet ◽

Experimental Conditions ◽

Phosphate Intake ◽

Dietary Restrictions ◽

Severe Reduction ◽

Male Wistar Rats ◽

Unrestricted Diet ◽

Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

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Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000654 ◽

2020 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Masoud Nasiri ◽

Saja Ahmadizad ◽

Mehdi Hedayati ◽

Tayebe Zarekar ◽

Mehdi Seydyousefi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Wistar Rats ◽

Enzymatic Antioxidants ◽

Acute Response ◽

Total Antioxidant ◽

Male Wistar Rats ◽

Exercise Induced ◽

And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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