scholarly journals Comparison of Ethanolic and Aqueous Populus balsamifera L. Bud Extracts by Different Extraction Methods: Chemical Composition, Antioxidant and Antibacterial Activities

2021 ◽  
Vol 14 (10) ◽  
pp. 1018
Author(s):  
Monika Stanciauskaite ◽  
Mindaugas Marksa ◽  
Lina Babickaite ◽  
Daiva Majiene ◽  
Kristina Ramanauskiene
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antibacterial Activities ◽  
Extraction Methods ◽  
Raw Material ◽  
Total Phenolic ◽  
Populus Balsamifera ◽  
Antioxidant Power ◽  
Balsam Poplar ◽  
Activity Effect

The balsam poplar (Populus balsamifera L.) buds that grow in Lithuania are a polyphenol-rich plant material with a chemical composition close to that of propolis. In order to potentially adapt the extracts of this plant’s raw material for therapeutic purposes, it is important to carry out detailed studies on the chemical composition and biological activity of balsam poplar buds. An important step is to evaluate the yield of polyphenols by different extraction methods and using different solvents. According to our research, extracts of balsam poplar buds collected in Lithuania are dominated by p-coumaric (496.9–13,291.2 µg/g), cinnamic acid (32.9–11,788.5 µg/g), pinobanksin (34.9–1775.5 µg/g) and salicin (215.3–1190.7 µg/g). The antioxidant activity of poplar buds was evaluated by the ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric-reducing antioxidant power) methods, all extracts showed antioxidant activity and the obtained results correlated with the obtained amounts of total phenolic compounds in the extracts (ABTS r = 0.974; DPPH r = 0.986; FRAP r = 0.955, p < 0.01). Studies of antimicrobial activity have shown that ethanolic extracts have an antimicrobal activity effect against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli. The extracts showed a better antimicrobal activity against gram-positive bacteria.

scholarly journals Extracts of Poplar Buds (Populus balsamifera L., Populus nigra L.) and Lithuanian Propolis: Comparison of Their Composition and Biological Activities

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 828
Author(s):  
Monika Stanciauskaite ◽  
Mindaugas Marksa ◽  
Mindaugas Liaudanskas ◽  
Liudas Ivanauskas ◽  
Marija Ivaskiene ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Populus Nigra ◽  
Raw Material ◽  
Populus Balsamifera ◽  
Plant Materials ◽  
Antioxidant Power ◽  
Balsam Poplar ◽  
Black Poplar

Balsam poplar and black poplar (Populus balsamifera L. and Populus nigra L.) buds that grow in Lithuania are the primary source of propolis, therefore it is proper to evaluate and compare the composition of these raw plant materials and propolis quantitatively and qualitatively. Propolis and balsamic poplar bud extract are dominated by p-coumaric acid and black poplar-caffeic acid. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (ferric-reducing antioxidant power) and CUPRAC (cupric reducing antioxidant capacity) methods and all extracts showed antioxidant activity, and obtained results correlated with the obtained amounts of phenolic compounds and flavonoids in the extracts. Studies of antimicrobial activity have shown that all extracts have a growth inhibitory effect against Staphylococcus aureus and Candida albicans, but the extract of balsam poplar buds showed the most significant effect of such kind. Considering the results of the research, it can be stated that balsam poplar buds cultured in Lithuania are the primary raw material of propolis, which is rich in phenolic compounds with antioxidant properties and is a promising raw material for pharmaceutical purposes.

Essential Oil and Hydrosol Extract Chemical Composition, Phytochemicals of Algerian Rhus pentaphylla Desf. and its Antioxidant Activity

2020 ◽  
Vol 16 (5) ◽  
pp. 627-638
Author(s):  
Nassima Benmansour ◽  
Asma Allal ◽  
Chaouki Selles ◽  
Boufeldja Tabti ◽  
Alain Muselli
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Chemical Compositions ◽  
Antioxidant Power ◽  
Promising Source

Background: Rhus pentaphylla Desf. (Anacardiaceae) widely grown in Algeria, is an underutilized plant and remains poorly studied. To investigate for the first time, the chemical composition profile of essential oil and hydrosol extract; besides Total Phenolic (TPC), Flavonoid (TFC) and Condensed Tannins Contents (CTC) of fruits, leaves and roots, the fatty acid composition was assessed. The radical scavenging activity of R. pentaphylla extracts was then evaluated. Methods: Essential oil and hydrosol extract were isolated respectively by hydrodistillation and liquidliquid- extraction from the aerial parts of R. pentaphylla. The antioxidant activity was evaluated using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical and ferric reducing antioxidant power (FRAP). Chemical compositions of various extracts were investigated using Gas Chromatography (GC) and GC-Mass Spectrometry (GC-MS). Results: The results of GC and GC-MS analysis revealed 83 components in essential oil and 75 components in hydrosol extract representing respectively 96.9% and 92.5% of the total extract composition. The main constituents were hexadecanoic acid (31.5%) followed by spathulenol (14.9%) in the oil while the higher amount present in the hydrosol extract was of spathulenol (14.2%). Otherwise, 13 and 18 fatty acids were identified in roots and fruits respectively. The highest levels of TPC and CTC were found in roots extracts while the highest quantity of TFC has been recorded in the leaves extract. Besides, hydrosol extract was able to scavenge DPPH and FRAP free radicals more efficiently than essential oil. Conclusion: R. pentaphylla contains bioactive substances that could be used as a new promising source of antioxidant compounds in different applications.

scholarly journals Chemical composition and antioxidant activity of geopropolis produced by Melipona fasciculata (Meliponinae) in flooded fields and cerrado areas of Maranhão State, northeastern Brazil

2016 ◽  
Vol 46 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Marisa Cristina Aranha BATISTA ◽  
Bruno Vinicius de Barros ABREU ◽  
Richard Pereira DUTRA ◽  
Mayara Soares CUNHA ◽  
Flavia Maria Mendonça do AMARAL ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Gallic Acid ◽  
Phenolic Acids ◽  
Ellagic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Northeastern Brazil ◽  
Total Phenolic ◽  
Antioxidant Power

ABSTRACT Geopropolis, a mixture of plant resin, wax, soil and salivary secretion, is produced by the stingless bee Melipona fasciculata. This aim of this study was to investigate the chemical composition and antioxidant activity of geopropolis collected from beehives in two phytogeographical regions, flooded fields and cerrado, in the municipalities of Palmeirândia and Fernando Falcão, Maranhão State, northeastern Brazil. The geopropolis compounds were identified by gas chromatography-mass spectrometry (GC/MS). Additionally, total phenolic content was determined with the Folin−Ciocalteu reagent and antioxidant activity was evaluated in vitro by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The four geopropolis samples varied in terms of total phenolic content and antioxidant activity and the highest values were observed for geopropolis from Fernando Falcão. Triterpenes such as cycloartane, ursane and oleanane and phenolic acids (protocatechuic acid and gallic acid) were identified in the geopropolis from Palmeirândia, while the phenolic acids, gallic and ellagic acid were the main compounds in geopropolis from Fernando Falcão. The antioxidant property of geopropolis is due to its high total phenolic content and predominance of gallic acid and ellagic acid. The results showed that the plant resources in two phytogeographical regions of Maranhão used by bees for the production of geopropolis contribute to the chemical composition and bioactivity of this product.

scholarly journals TOTAL PHENOLIC CONTENT AND CHEMICAL COMPOSITION OF LITHUANIAN BALSAM POPLAR BUDS (POPULUS BALSAMIFERA L.) ETHANOLIC AND AQUEOUS EXTRACTS

InterConf ◽  
2021 ◽  
pp. 214-218
Author(s):  
Monika Stanciauskaite ◽  
Daiva Majiene ◽  
Liudas Ivanauskas ◽  
Kristina Ramanauskiene
Keyword(s):  
Chemical Composition ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Populus Balsamifera ◽  
Balsam Poplar ◽  
Ethanolic Extracts

The aim of this study was to prepare liquid aqueous and ethanolic extracts of poplar buds and to investigate their chemical composition.

scholarly journals Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
THEERAPHAN CHUMROENPHAT ◽  
Surapon Saensouk ◽  
PIYAPORN SAENSOUK
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii,  TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

scholarly journals The Effects of Different Extraction Methods on Antioxidant Properties, Chemical Composition, and Thermal Behavior of Black Seed (Nigella sativaL.) Oil

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Nameer Khairullah Mohammed ◽  
Mohd Yazid Abd Manap ◽  
Chin Ping Tan ◽  
Belal J. Muhialdin ◽  
Amaal M. Alhelli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Thermal Behavior ◽  
Nigella Sativa ◽  
Antioxidant Properties ◽  
Natural Antioxidants ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Black Seeds ◽  
High Level

TheNigella sativaL. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine.N.sativaseeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in theNigella sativaoil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.

scholarly journals Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3083 ◽  
Author(s):  
Chawalit Yongram ◽  
Bunleu Sungthong ◽  
Ploenthip Puthongking ◽  
Natthida Weerapreeyakul
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Composition Analysis ◽  
Cytotoxic Activities ◽  
Antioxidant Power ◽  
Dipterocarpus Alatus

Dipterocarpus alatus (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from D. alatus are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography–mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content.

scholarly journals Total Phenolic, Total Flavonoids Content and Antioxidant Activity of Mangifera sp. Leaf Extracts

2020 ◽  
Vol 11 (1S) ◽  
Author(s):  
Athilah Farhanah Ahmad Fuad ◽  
Afnani Alwi@Ali ◽  
Nurul Asma Hasliza Zulkifly ◽  
Noor Asidah Mohamed
Keyword(s):  
Antioxidant Activity ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Mangifera sp. is a versatile plant that was reported to have various bioactivities, however only the fruits have gain popularity due to it sweet flesh and been known worldwide. It has a potential source of flavonoids and carotenoids, which makes them a nutritious functional food to consume.  This study focused on determination of the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity of the Mangifera sp. leaves extract in different water extraction methods. The TPC and TFC was determined by using Folin-Ciocalteu method and aluminium chloride method respectively while antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and ferric reducing antioxidant power (FRAP) assay. Soxhlet extraction (SXE) produced the highest extraction yield compared to microwave extraction (MWE).  MWE at 8 minutes extract showed the highest TPC (262.13±0.05 mg GAE/g). SXE showed the highest TFC (413.46±0.77 mg rutin/g). The highest antioxidant activity was recorded in MWE 8 minutes extract through DPPH and FRAP assays compared to other two MWE extract and SXE extract.

scholarly journals Comparative Study of Chemical Composition and Antioxidant Activity of Essential Oils and Crude Extracts of Four Characteristic Zingiberaceae Herbs

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 501
Author(s):  
Milena Ivanović ◽  
Kaja Makoter ◽  
Maša Islamčević Razboršek
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Curcuma Longa ◽  
Water Extract ◽  
Chemical Components ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Crude Extracts

The ginger family (Zingiberaceae) includes plants that are known worldwide to have a distinctive smell and taste, which are often used as spices in the kitchen, but also in various industries (pharmaceutical, medical, and cosmetic) due to their proven biological activity. The aim of this study was to investigate and compare the chemical composition and antioxidant activity (AA) of essential oils (EOs) of four characteristic ginger species: Elettaria cardamomum L. Maton (cardamom), Curcuma Longa L. (turmeric), Zingiber Officinale Roscoe (ginger), and Alpinia Officinarum Hance (galangal). Furthermore, the total phenolic content (TPC) and AA of crude extracts obtained after using ultrasound-assisted extraction (UAE) and different extraction solvents (80% ethanol, 80% methanol and water) were evaluated. A total of 87 different chemical components were determined by GC-MS/MS in the EOs obtained after hydrodistillation, 14 of which were identified in varying amounts in all EOs. The major compounds found in cardamom, turmeric, ginger, and galangal were α-terpinyl acetate (40.70%), β-turmerone (25.77%), α-zingiberene (22.69%) and 1,8-cineol (42.71%), respectively. In general, 80% ethanol was found to be the most effective extracting solvent for the bioactivities of the investigated species from the Zingiberaceae family. Among the crude extracts, ethanolic extract of galangal showed the highest TPC value (63.01 ± 1.06 mg GA g−1 DW), while the lowest TPC content was found in cardamom water extract (1.04 ± 0.29 mg GA g−1 DW). The AA evaluated by two different assays (ferric-reducing antioxidant power-FRAP and the scavenging activity of the cationic ABTS radical) proved that galangal rhizome is the plant with the highest antioxidant potential. In addition, no statistical difference was found between the AA of turmeric and ginger extracts, while cardamom rhizome was again inferior. In contrast to the crude extracts, the EOs resulted in significantly lower ABTS and FRAP values, with turmeric EO showing the highest AA.

scholarly journals CHEMICAL COMPOSITION, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF TWO MOROCCAN THYMUS ESSENTIAL OILS

2019 ◽  
Vol 12 (1) ◽  
pp. 447 ◽  
Author(s):  
Imane Aouam ◽  
Yassine El Atki ◽  
Amal Taroq ◽  
Fatima El Kamari ◽  
Abdelfattah Abdellaoui
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Bornyl Acetate ◽  
Total Phenolic ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Antioxidant Power

Objective: The aim of the present study is to investigate chemical constituents of Thymus riatarum and Thymus blecherianus essential oils (EOs) as well as to evaluate, for the first time, their antioxidant effect and antibacterial activity against six bacterial strains responsible for nosocomial infections.Methods: The chemical composition of EOs was analyzed using gas chromatography (GC) and GC-mass spectrometry, the antibacterial capacity of the two thymus species was evaluated against six bacteria species: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Acinetobacter baumannii, and Citrobacter sp. using disk diffusion method and microdilution assay. Finally, the antioxidant activity was measured by four different test systems of assay, namely free radical scavenging activities, ferric reducing/antioxidant power assays, total phenolic, and flavonoid contents.Results: A total of 15 compounds representing 99.6% of T. riatarum oil were identified with thymol (28.8%), borneol (20.0%), and α-phellandrene (13.0%) as the main constituents. Eleven components of T. blecherianus were identified representing 98.2% of the total oil composition; the most abundant constituents were as follows: Carvacrol (45.9%), bornyl acetate (20.1%), and borneol (15.7%). Strong antibacterial activity of the two EOs was identified against all bacterial strains tested. Concerning the antioxidant results, T. riatarum EO exhibited higher antioxidant activity than T. blecherianus in the three assays with an IC50 value equal to 5.75±0.06 mg/ml, which was probably due to its high content of polyphenols (28.95±0.13 mg GAE/g DW). Total flavonoid content was found equal for the two EOs.Conclusion: EO of T. riatarum and T. blecherianus from Morocco can be exploited as a natural antibacterial and antioxidant new potential sources.

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