scholarly journals Fast-Tracking Isolation, Identification and Characterization of New Microalgae for Nutraceutical and Feed Applications

Phycology ◽  
2022 ◽  
Vol 2 (1) ◽  
pp. 86-108
Author(s):  
Boer Bao ◽  
Skye R. Thomas-Hall ◽  
Peer M. Schenk

Microalgae contain high-value biochemical compounds including fatty acids (FA), protein and carotenoids, and are promising bioresources to enhance nutrition of food and animal feed. Important requirements for commercial strains are rapid growth and high productivities of desirable compounds. As these traits are believed to be found in aquatic environments with fluctuating conditions, we collected microalgae from marine and freshwater environments that are subjected to eutrophication and/or tidal fluctuations. Using this directed approach, 40 monoalgal cultures were isolated and 25 identified through 18S rDNA sequencing and morphological characterization. Based on their high growth rates (0.28–0.60 day−1) and biomass productivities (0.25–0.44 g L−1day−1) in commercial fertilizer under standardized conditions, six new strains were selected. Scenedesmus sp. GW63 produced quality FA-rich biomass with high omega-3 polyunsaturated FA (28.5% of total FA (TFA)) contents, especially α-linolenic acid (ALA; 20.0% of TFA) with a very low n-6/n-3 ratio (0.4), and high FA productivity (32.6 mg L−1 day−1). A high protein productivity (34.5 mg L−1 day−1) made Desmodesmus sp. UQL1_26 (33.4% of dry weight (DW)) attractive as potential protein-rich feed and nutrition supplement. Monoraphidium convolutum GW5 displayed valuable carotenoid production (0.8% DW) with high carotenoid accumulation capability (0.8 mg L−1 day−1). This research provides a pathway for fast-tracking the selection of high-performing local microalgae from different environments for nutraceuticals, functional foods and animal feed applications.

2019 ◽  
Vol 5 (2) ◽  
pp. 77-88 ◽  
Author(s):  
E.C. Fitches ◽  
M. Dickinson ◽  
D. De Marzo ◽  
M.E. Wakefield ◽  
A.C. Charlton ◽  
...  

The productivity of Musca domestica larvae reared on poultry litter and the nutritional parameters of insect meal as an animal feed ingredient have been evaluated. In laboratory bioassays, a seeding density of 0.32 g eggs/kg wet weight litter produced maximal yields of >50 g of naturally egressing larvae/kg substrate. Substrate composition and moisture content were critical determinants for insect development and generally litter wetted to water holding capacity (approx. 23% dry weight) was optimal for larval production. Four different processing methods were evaluated, but had no significant effects upon the nutritional quality of insect meals and all were effective in reducing microbial (Enterobacteriacae) populations to levels considered acceptable by feed regulations. Processed samples contained an average of 59.9% protein and 24.2% fat. Differences in levels of fatty acids, particularly palmitic, linoleic and α-linolenic were observed in larvae reared on different substrates. By contrast, highly similar amino acid profiles were derived from larvae reared on different substrates. With well-balanced amino acid profiles, superior to soymeal and more comparable to fishmeal, M. domestica larval meals have considerable potential for use as a protein-rich animal feed.


Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 310
Author(s):  
Fabian Sandgruber ◽  
Annekathrin Gielsdorf ◽  
Anja C. Baur ◽  
Benjamin Schenz ◽  
Sandra Marie Müller ◽  
...  

The nutrient composition of 15 commercially available microalgae powders of Arthrospira platensis, Chlorella pyrenoidosa and vulgaris, Dunaliella salina, Haematococcus pluvialis, Tetraselmis chuii, and Aphanizomenon flos-aquae was analyzed. The Dunaliella salina powders were characterized by a high content of carbohydrates, saturated fatty acids (SFAs), omega-6-polyunsaturated fatty acids (n6-PUFAs), heavy metals, and α-tocopherol, whereas the protein amounts, essential amino acids (EAAs), omega-3-PUFAs (n3-PUFAs), vitamins, and minerals were low. In the powder of Haematococcus pluvialis, ten times higher amounts of carotenoids compared to all other analyzed powders were determined, yet it was low in vitamins D and E, protein, and EAAs, and the n6/n3-PUFAs ratio was comparably high. Vitamin B12, quantified as cobalamin, was below 0.02 mg/100 g dry weight (d.w.) in all studied powders. Based on our analysis, microalgae such as Aphanizomenon and Chlorella may contribute to an adequate intake of critical nutrients such as protein with a high content of EAAs, dietary fibers, n3-PUFAs, Ca, Fe, Mg, and Zn, as well as vitamin D and E. Yet, the nutritional value of Aphanizomenon flos-aquae was slightly decreased by high contents of SFAs. The present data show that microalgae are rich in valuable nutrients, but the macro- and micronutrient profiles differ strongly between and within species.


2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.


Author(s):  
Kulyash Meiramkulova ◽  
Gulmira Adilbektegi ◽  
Galym Baituk ◽  
Aigul Kurmanbayeva ◽  
Anuarbek Kakabayev ◽  
...  

Waste recovery is an important aspect towards human and environmental health protection. Unfortunately, proper food waste management is among the serious challenges in the field of solid waste management worldwide. Therefore, it is of great importance to conduct studies towards achieving efficient and cost-effective approaches for food waste management. This study investigated the potential of recovering food waste through maggots’ production as animal feed. The influence of fly attractant application on maggot production was also investigated. The study also investigated the potential of maggot production for waste recovery and reduction. Four different types of food waste (starch food leftovers, rotten bananas and peels, rotten pineapple and peels, and rotten oranges) were used in the investigation process. From the results, it was observed that the application of fly attractants had a significant effect on the production of maggots as determined by the weights after harvesting. Average weight of 94 g/kg of maggot was achieved from banana materials with an application of fly attractant during the 8th day of the cultivation; which is equivalent to a 32.4% increase from the same day when the material was cultured without applying fly attractant. Also, from the starch materials, about 77 g/kg of maggot weight was achieved; which is a 54.6% increase from the same day and the same material but without application of fly attractant. Moreover, the relative dry weight reduction in the trials varied from 52.5% to 82.4%.


Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 310 ◽  
Author(s):  
Shakir Ullah ◽  
Jafar Khan ◽  
Khizar Hayat ◽  
Ahmed Abdelfattah Elateeq ◽  
Uzma Salam ◽  
...  

Trace metals (TM) contamination is a severe problem in the environment and produced an adverse effect on the productivity of crops. Cadmium (Cd) is a TM ranked seven among the top 20 pollutants due to its high toxicity and solubility in water, taken up by the plants and affects their growth and metabolism. In this study, we evaluated the growth, Cd accumulation and tolerance capacities of three chickpea (Cicer arietinum L.) cultivars (NC234 (NC2), ICCV89310 (IC8) and ICCV89323-B (IC8-B)), subjected to two Cd concentrations (25 and 50 µM) in hydroponic culture. The toxicity of Cd reduced the plant height and fresh and dry biomass in all cultivars. The maximum reduction was observed at 50 µM of Cd. Compared with IC8-B, cultivars IC8 and NC2 exhibited better performance with high growth, biomass, root to shoot (R/S) ratio and water content under high Cd stress. To measure the accumulation of Cd in root and shoot, an inductively coupled plasma optical emission spectrometer (ICP-OES) was used. IC8 and NC2 had comparatively high Cd tolerance and accumulation ability (> 100 µg g−1 dry weight), with IC8 being more tolerant and accumulated higher Cd in shoot than NC2, while cultivar IC8-B was sensitive. Root accumulated more Cd than shoot in a dose-dependent manner. The bioconcentration factors (BCF) and bioaccumulation coefficients (BAC) were far higher than one (> 1) and increased with an increase in Cd concentrations, while the translocation factor (TF) was less than one (< 1), suggesting that all the three cultivars were unable to transfer Cd from the root to the shoot efficiently. Our results indicated that IC8 and NC2 proved to be resistant, while IC8-B showed sensitivity when exposed to high Cd stress (50 µM).


Poljoprivreda ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 48-55
Author(s):  
Tatjana Krička ◽  
Nikola Bilandžija ◽  
Zlatko Janječić ◽  
Mateja Grubor ◽  
Dalibor Bedeković ◽  
...  

The most important aspect of broiler production is feeding, whose costs represent about 70% of total costs. Thus, the growth of poultry production has been based on a strong consumer demand for products that are cheap, safe, and healthy. That kind of product is old bread, whose return from the store represent a problem for its remediation. For the use of the old bread as a feed, the European Union has promulgated a series of regulations and directives, which order a ban on its utilization without prior treatment. The most common treatments are pelleting and extrusion. In poultry, the upper limit for the use of the old bread in feedstuff is often prescribed and amounts to 15%. The aim of this paper was to determine a possibility to use 5 and 10% old brown pelleted bread in feed mixtures for broilers fattening. The study has shown that the addition of old bread significantly improves broiler production indicators. The best results and a more favorable ratio of omega 6 and omega 3 fatty acids in the chicken muscles were obtained in the group fed with a 10‐percent share of old brown bread (p&lt;0.05). Such a processed old bread can be used as a new ingredient in animal feed production as a partial replacement for maize component.


2017 ◽  
Vol 45 (2) ◽  
pp. 323-331 ◽  
Author(s):  
Dimitrios BILALIS ◽  
Ioannis ROUSSIS ◽  
Francisco FUENTES ◽  
Ioanna KAKABOUKI ◽  
Ilias TRAVLOS

Climate change is the greatest environmental threat facing humanity worldwide. Areas of South-East Europe and Mediterranean basin are expected to be among the most vulnerable countries to climate change. As a result of climate change, new species and crops have been introduced and may be introduced in the coming years. In addition, FAO considers that Organic Agriculture is an effective mitigation strategy to climate change and can build robust soils that adapt better to weather extremes associated with climate change. This review provides an overview of the growth performance of new innovative crops, including chia, camelina, quinoa, teff and nigella and retrovative crops such as flax and emmer wheat, based on experimental investigations conducted under Mediterranean conditions and organic cropping system. Several studies, performed under organic system, have proved that innovative crops can also be grown for alternative uses. Quinoa and chia could be successfully used in animal feed. Moreover, quinoa could be exploited as a medicinal plant due to saponins extracted from seed coats. Nigella and camelina seeds contain oils which can have several uses in pharmaceutical and food industries. Flax seed oil is rich in omega-3 fatty acids and can be accepted in the diets designed for specific health benefits. According to the literature, it is observed that innovative crops cultivated under organic system present better quality and similar yields as with those cultivated under conventional system, and in some cases, even higher. Taking all these into account, organic agriculture could also be characterized as innovative and not only as traditional.


2013 ◽  
Vol 76 (6) ◽  
pp. 1021-1026 ◽  
Author(s):  
MAYUMI HACHINOHE ◽  
KEITAROU KIMURA ◽  
YUJI KUBO ◽  
KATSUO TANJI ◽  
SHIOKA HAMAMATSU ◽  
...  

We investigated the fate of radioactive cesium (134Cs plus 137Cs) during the production of tofu, natto, and nimame (boiled soybean) from a contaminated Japanese soybean cultivar harvested in FY2011. Tofu, natto, and nimame were made from soybean grains containing radioactive cesium (240 to 340 Bq/kg [dry weight]), and the radioactive cesium in the processed soybean foods and in by-product fractions such as okara, broth, and waste water was measured with a germanium semiconductor detector. The processing factor is the ratio of radioactive cesium concentration of a product before and after processing. For tofu, natto, nimame, and for the by-product okara, processing factors were 0.12, 0.40, 0.20, and 0.18, respectively; this suggested that these three soybean foods and okara, used mainly as an animal feed, can be considered safe for human and animal consumption according to the standard limit for radioactive cesium of soybean grains. Furthermore, the ratio of radioactive cesium concentrations in the cotyledon, hypocotyl, and seed coat portions of the soybean grain was found to be approximately 1:1:0.4.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 66-66

Abstract Growing concerns about antibiotic resistance coupled with recent legislation limiting in-feed antibiotic use in livestock feed has led to a resurgence of interest in plant-based extracts, prebiotics/probiotics, and other nutraceutical compounds with immune-modulating properties. Consumer preference for functional ingredients in their own food as well as feed for their pets or livestock has created a niche for development of feed additives with bioactive properties with the potential to improve health through their ability to regulate inflammation and modulate microbial communities. For example, dietary omega-3 fatty acids have been documented to reduce inflammation through altering the fatty acid composition of immune cells, shifting immune cell function, and reducing inflammatory lipid mediator and cytokine production. Dietary probiotic supplementation allows an intimate interaction between the gut mucosa and the largest concentrated population of the host’s immune cells, the mucosal immune system. Probiotics have been shown to modulate the inflammatory response through interaction with intestinal epithelial cells, M-cells in Peyer’s patches, innate immune cells such as dendritic and other antigen-presenting cells, and increasing barrier function through increased mucus and antibody production. Other feedstuffs such as green tea components (epigallocatechin-3-gallate, EGCG) interact with neutrophils and macrophages to limit cellular migration and inflammatory molecule production to reduce inflammation. A wealth of information detailing mechanistic outcomes exists in mouse literature and human cell lines, while livestock and companion animal literature is an untapped area for future work. An emphasis on research aimed at a more molecular, mechanistic understanding of currently available feed additives will lead to the improvement of existing additives and development of new additives for use in livestock and companion animal feed.


2020 ◽  
Vol 6 (2) ◽  
pp. 95-109 ◽  
Author(s):  
G. Bosch ◽  
D.G.A.B. Oonincx ◽  
H.R. Jordan ◽  
J. Zhang ◽  
J.J.A. van Loon ◽  
...  

Using larvae of the black soldier fly (Hermetia illucens; BSF) to convert low-value residual organic resources into high-value products like protein-rich animal feed ingredients and biofuel while managing organic waste has developed into a global industry. Considering the associated exponential increase in publications dealing with diet conversion efficiency by BSF larvae, it is timely to suggest procedures to arrive at an improved harmonization and reproducibility among studies. This means establishing protocols for describing the basic experiment design, fly colony origin, rearing procedures, reference and experimental feeding substrates, and sampling preparations including microbiota and chemical analyses. Such standardised protocols are instrumental to allow conversion efficiencies to be calculated. Some of these parameters are relatively easy to describe such as giving the origin and rearing conditions, while others are more challenging (e.g. description of microbe community). In this article we discuss and propose such procedures with the aim to arrive at standardisation of how future resource conversion studies with BSF larvae are conducted and how results are communicated.


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