scholarly journals Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1481
Author(s):  
Florian Hahn ◽  
Laura Sanjurjo Sanjurjo Loures ◽  
Caroline A. Sparks ◽  
Kostya Kanyuka ◽  
Vladimir Nekrasov
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Genome Editing ◽  
Plant Viruses ◽  
Targeted Mutagenesis ◽  
Experimental Methodology ◽  
Yellow Mosaic Virus ◽  
Wheat Varieties ◽  
Mutant Lines ◽  
Winter Wheat Varieties

CRISPR/Cas technology has recently become the molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. Primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. While BAK1 is an important regulator of plant immunity and development, Ta-eIF4E and Ta-eIF(iso)4E act as susceptibility (S) factors required for plant viruses from the Potyviridae family to complete their life cycle. We anticipate the resultant homozygous tabak1-2 mutant lines will facilitate studies on the involvement of BAK1 in immune responses in wheat, while ta-eif4e and ta-eif(iso)4e mutant lines have the potential to become a source of resistance to wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV), both of which are important pathogens of wheat. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

scholarly journals Efficient CRISPR/Cas-mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

2021 ◽  
Author(s):  
Florian Hahn ◽  
Laura Sanjurjo Loures ◽  
Caroline A. Sparks ◽  
Kostya Kanyuka ◽  
Vladimir Nekrasov
Keyword(s):  
Winter Wheat ◽  
Genome Editing ◽  
Crop Improvement ◽  
Research Community ◽  
Targeted Mutagenesis ◽  
Experimental Methodology ◽  
Wheat Varieties ◽  
Transgenic Lines ◽  
Staple Crop ◽  
Winter Wheat Varieties

The CRISPR/Cas technology has recently become a molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. The primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

scholarly journals Ribotypes of Polymyxa graminis in Wheat Samples Infected with Soilborne Wheat Viruses in China

Plant Disease ◽  
2018 ◽  
Vol 102 (5) ◽  
pp. 948-954 ◽  
Author(s):  
Yu Xu ◽  
Lifeng Hu ◽  
Linying Li ◽  
Yan Zhang ◽  
Bingjian Sun ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Mosaic Virus ◽  
Wheat Root ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Wheat Yellow Mosaic Virus ◽  
Polymyxa Graminis ◽  
Obvious Association ◽  
Soilborne Viruses ◽  
Chinese Wheat

Polymyxa graminis is an obligate parasite and important vector of more than 14 soilborne plant viruses that pose a significant threat to cereal crops in Europe, North America, and Asia. Different ribotypes or formae speciales of P. graminis have been recognized and these may be associated with different cereal hosts or with transmission of different viruses. Two soilborne viruses infecting winter wheat in China have been reported and well studied (Wheat yellow mosaic virus [WYMV, genus Bymovirus] and Chinese wheat mosaic virus [CWMV, genus Furovirus]) but there has been no reported characterization of P. graminis isolates associated with them. In this study, the ribosomal DNA internal transcribed spacer (ITS) regions of P. graminis were examined from 63 wheat samples with apparent virus symptoms obtained from 16 sites within six Chinese provinces. Their associations with soilborne viruses were investigated. Ribotype I (P. graminis f. sp. temperata) and ribotype II (P. graminis f. sp. tepida) were confirmed in winter wheat regions of China for the first time. All 63 wheat root samples were infected with ribotype I of P. graminis and 11 were also infected with ribotype II. There was no obvious association between the ribotypes and infection by either WYMV or CWMV (or double infection). Phylogenetic analysis of the P. graminis ITS1-5.8S-ITS2 sequences revealed that ribotype I in China belongs to previously reported subgroup Ib, whereas ribotype II belongs to IIa. There was considerable sequence variation (pairwise distances from 0.0219 to 0.0319) between Chinese ribotype I isolates of different regions and previously reported ribotype I isolate Ken5 (accession number HE860055.1).

QUALITATIVE STABILITY OF WINTER WHEAT VARIETIES AT DIFFERENT LOCATIONS

2000 ◽  
Vol 48 (2) ◽  
pp. 191-195
Author(s):  
C. Kondora ◽  
M. Szabó ◽  
A. Máté ◽  
G. Szabó
Keyword(s):  
Quality Control ◽  
Winter Wheat ◽  
Evaluation Method ◽  
Wheat Varieties ◽  
Local Conditions ◽  
Grain Yields ◽  
Baking Quality ◽  
Small Plot ◽  
High Yielding Varieties ◽  
Winter Wheat Varieties

Owing to the significant differences in the adaptability of state-registered varieties, those which can adapt well to the local conditions should be given preference. There are several high-yielding varieties available in Hungary with excellent agronomic properties, good adaptability and satisfactory baking quality. This study was conducted to analyse the adaptability of 34 state-registered winter wheat varieties tested in the small plot trials of the National Institute for Agricultural Quality Control (NIAQC) at 5–9 locations between 1994 and 1997 based on their gluten quantity and farinographic index. For the comparison of the varieties the evaluation method of Eberhart and Russell (1966) was applied as modified by Bedő and Balla (1977). The qualitative stability and adaptability values of the varieties differ from the adaptability and stability values calculated from the grain yields. Some winter wheat varieties have good qualitative adaptability and stability, while others have special adaptability and poor qualitative stability, but the majority of the varieties do not belong to these groups.

Impact of the estimated doses of mineral fertilizers on the infestation mildew, pyrenophora and yield of winter wheat varieties

2020 ◽  
Author(s):  
A.Y. Ozheredova ◽  
◽  
A. N. Esaulko ◽  
L.A. Mikhno ◽  
A.P. Shutko ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Winter Wheat ◽  
Mineral Fertilizers ◽  
Wheat Varieties ◽  
Leached Chernozem ◽  
Winter Wheat Varieties

The article presents materials on the influence of calculated doses of mineral fertilizers on the incidence of powdery mildew, pyrenophorosis and yield of winter wheat varieties cultivated on leached Chernozem for 2016-2018.

Varietal potential of winter wheat in the Kursk region

2020 ◽  
Author(s):  
Pigorev I. Ya. Pigorev I. Ya. ◽  
◽  
V.A. Kudinov V.A. ◽  
I.V. Ichkov I.V.
Keyword(s):  
Small Business ◽  
Winter Wheat ◽  
Industrial Complex ◽  
Wheat Varieties ◽  
Business Entities ◽  
Kursk Region ◽  
Winter Wheat Varieties ◽  
Large Enterprises

the range of winter wheat varieties in the conditions of sown areas of the Kursk region is considered. The analysis of the varieties used with the priorities and volumes of crops in large enterprises and small business entities of the agro-industrial complex is given.

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