Bioactive Compounds in Sarcocornia and Arthrocnemum, Two Wild Halophilic Genera from the Iberian Peninsula

(1) Background: this study describes bioactive compounds in the following halophytes: Sarcocornia (S. alpini, S. pruinosa, and S. perennis) and Arthrocnemum (A. macrostachyum). The material comes from: coastal marshes in Tinto River, Guadiana River, and some interior provinces from the Iberian Peninsula. (2) Methods: the techniques used were Folin–Ciocalteu, GC-MS, and ESI-MS/MS. (3) Results: Five phenolic acids were found in Sarcocornia: trans-cinnamic, salicylic, veratric, coumaric, and caffeic acids. In addition, in Arthronemum, ferulic acid was also detected. The obtained flavonoids were cyanidin-3-O-arabinoside, luteolin-7-glucoside, dihydroquercetin, and p-coumaroyl-glucoside. They also presented fatty acids, such as palmitic, linoleic, and oleic acids in Sarcocornia, while palmitic, linolenic, and stearic acids were the main fatty acids in A. macrostachyum. (4) Conclusions: the high diversity of the compounds identified confirms the relation between nutritional interest and salt tolerance in halophytes.

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Bioactive Compounds in Salicornia patula Duval-Jouve: A Mediterranean Edible Euhalophyte

Foods ◽

10.3390/foods10020410 ◽

2021 ◽

Vol 10 (2) ◽

pp. 410

Author(s):

Irene Sánchez-Gavilán ◽

Esteban Ramírez ◽

Vicenta de la Fuente

Keyword(s):

Fatty Acids ◽

Bioactive Compounds ◽

Mediterranean Area ◽

Biological Properties ◽

Total Phenolic ◽

Tinto River ◽

New Findings ◽

High Concentrations ◽

Salicornia Patula ◽

First Time

Many halophytes have great nutritional and functional potential, providing chemical compounds with biological properties. Salicornia patula Duval-Jouve is a common euhalophyte from saline Mediterranean territories (Spain, Portugal, France, and Italy). In the present work we quantified for the first time the bioactive compounds in S. patula (total phenolic compounds and fatty acids), from Iberian Peninsula localities: littoral-coastal Tinto River basin areas (southwest Spain, the Huelva province), and mainland continental territories (northwest and central Spain, the Valladolid and Madrid provinces). Five phenolic acids including caffeic, coumaric, veratric, salicylic, and transcinnamic have been found with differences between mainland and coastal saltmarshes. S. patula contain four flavonoids: quercetin-3-O-rutinoside, kaempferol/luteolin, apigenin 7-glucoside, and pelargonidin-3-O-rutinoside. These last two glycosylated compounds are described for the first time in this genus of Chenopodiaceae. The fatty acid profile described in S. patula stems contains palmitic, oleic, and linoleic acids in high concentrations, while stearic and long-chain fatty acids were detected in low amounts. These new findings confirm that S. patula is a valuable source of bioactive compounds from Mediterranean area.

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Cold pressed versus refined winterized corn oils: quality, composition and aroma

Grasas y Aceites ◽

10.3989/gya.1168162 ◽

2017 ◽

Vol 68 (2) ◽

pp. 194 ◽

Cited By ~ 2

Author(s):

B. Aydeniz Güneşer ◽

E. Yılmaz ◽

S. Ok

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Phenolic Acids ◽

Sensory Attributes ◽

Saponification Number ◽

Total Carotenoid ◽

Quality Improvements ◽

Phenolic Contents ◽

Cold Pressed

The aims of this study were to characterize and compare cold pressed and fully refined winterized corn oils. Free fatty acidity (FFA), peroxide (PV) and p-anisidin (p-AV) values, saponification number, total carotenoid and phenolic contents of cold pressed corn oils were higher than that of the refined winterized corn oils. Linoleic and oleic acids (approximately 53-54% and 30-31%, respectively) were detected as the major fatty acids in both oil samples. Fifteen different sterols with a majority of β-sitosterol, campesterol, and stigmasterol were quantified in both oil samples. Although phenolic compounds were not quantified in the refined winterized oil samples, some flavonoids (hesperidin, rutin) and phenolic acids (gallic, syringic, rosmaniric and trans-ferulic) were detected in the cold pressed oil samples. This study concludes that cold pressed corn oils could be superior in terms of bioactive compounds but still need some quality improvements for sensory attributes.

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Analysis of Distribution of Selected Bioactive Compounds in Camelina sativa from Seeds to Pomace and Oil

Agronomy ◽

10.3390/agronomy9040168 ◽

2019 ◽

Vol 9 (4) ◽

pp. 168 ◽

Cited By ~ 7

Author(s):

Danuta Kurasiak-Popowska ◽

Bernadetta Ryńska ◽

Kinga Stuper-Szablewska

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Bioactive Compounds ◽

Phenolic Acids ◽

Food Industry ◽

Seed Oil ◽

Unsaturated Fatty Acids ◽

Camelina Sativa ◽

The Other ◽

Camelina Oil

Camelina sativa is an oilseed plant that produces seed oil rich in vitamins, UFA (unsaturated fatty acids), phytosterols, and polyphenols. Most, but not all, bioactive compounds are soluble in oil. So far, studies have been based analyzing the profile of bioactive compounds only in oil. As part of this work, it was decided to examine the seeds, oil, and pomace of four genotypes of Camelina sativa (three spring genotypes and one winter cultivar). The transmission of bioactive compounds to oil and pomace was compared to their content in seeds. The quantitative profile of selected bioactive compounds was analyzed: eight flavonoid aglycons, 11 phenolic acids, three carotenoids, and 19 fatty acids. As a result of pressing more than 80% of flavonoids entered oil, whereas 20% remained in the pomace. When the content of phenolic acids in seeds and in oil was compared, it turned out that on average 50% of these compounds entered oil. Among all the phenolic acids under analysis the highest contents of chlorogenic, caffeic, and sinapic acids were found both in camelina seeds and oil. Similarly to the other bioactive compounds, more than 70% of fat-soluble carotenoids were extracted from camelina seeds with oil. Most of the genotypes had the fatty acid composition and the ω-6/ω-3 ratio characteristic of camelina oil. Camelina oil is characterized by properties that enable its extensive use in the food industry.

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Cold-pressed pumpkin seed (Cucurbita pepo L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds

Grasas y Aceites ◽

10.3989/gya.0668171 ◽

2018 ◽

Vol 69 (1) ◽

pp. 232 ◽

Cited By ~ 7

Author(s):

G. Akin ◽

F. N. Arslan ◽

S. N. Karuk Elmasa ◽

I. Yilmaz

Keyword(s):

Fatty Acids ◽

Bioactive Compounds ◽

Phenolic Acids ◽

Cucurbita Pepo ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Central Anatolia ◽

Seed Oils ◽

Pumpkin Seed ◽

Cold Pressed

There is a growing interest in cold-pressed oils because they present high contents of bioactive compounds. These oils have the characteristic properties of seeds and are specific products of their regions. The aim of this study was to determine the compositions and contents of fatty acids, phytosterols, squalene, tocols, phenolic acids, carotenoids and phenolic bioactives, and the free radical scavenging as well as antioxidant activities of cold-pressed pumpkin (Cucurbita pepo L.) seed oils. Oil samples from raw pumpkin seeds cultivated in four different central Anatolia regions of Turkey were prepared using a laboratory screw-pressing machine. The results indicate that cold-pressed pumpkin seed oils (PSO) have excellent quality with high contents of polyunsaturated fatty acids (%ΣPUFAs) (53.60 ±0.06-53.73 ±0.05), total phytosterols (782.1±9.7–805.2 ±11.3 mg/100 g oil), squalene (591.3±10.6–632.5±11.4 mg/100 g oil), tocols (97.79 ±0.76?94.29 ±0.34 mg/100 g oil), phenolic acids (22.73 ±0.41–23.98 ±0.46 mg/100 g oil), carotenoids (6.95 ±0.03–7.60 ±0.03 mg/100 g oil), total phenolics (3.96 ±0.13 –5.82±0.15 mg GAE/100 g), free radical-scavenging activity (5.70 ±0.13?7.35 ±0.15 mg GAE/100 g) and total antioxidant activity (26.67±0.97-38.89±1.41 mg GAE/100 g) values. Thus, this study demonstrates that the cold- pressed PSOs from the central Anatolia regions of Turkey are an excellent source of natural bioactive compounds, free of chemical contaminants and nutritious.

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PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTI-CYTOTOXIC AND ANTICANCER EFFECTS OF GALINSOGA PARVIFLORA AND VERNONIA POLYANTHES (ASTERACEAE) EXTRACTS

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v8.i10.2020.1782 ◽

2020 ◽

Vol 8 (10) ◽

pp. 84-98

Author(s):

Sula M. V. Feleti ◽

Renê L. Aleluia ◽

Suiany V. Gervásio ◽

Jean Carlos V. Dutra ◽

Jessica R. P. Oliveira ◽

...

Keyword(s):

Fatty Acids ◽

Phenolic Acids ◽

Unsaturated Fatty Acids ◽

Biological Effects ◽

Human Lymphocytes ◽

Chemical Characterization ◽

Total Content ◽

Ags Cells ◽

Ft Icr Ms ◽

Ft Icr

The study was designed to investigate the chemical composition and the biological effects of G. parviflora and V. polyanthes ethanolic extracts in vitro. Total content of phenols, flavonoids and tannins was quantified by spectrophotometry; chemical characterization was permed by mass spectrometry (ESI (-) FT-ICR MS and APCI (+) FT-ICR MS analysis). Antioxidant activities were determined by FRAP and Fe2+ chelating methods. Extracts cytotoxicity was evaluated in human lymphocytes, sarcoma-180 (S-180) and human gastric adenocarcinoma (AGS) cells, by MTT assay. V. polyanthes presented higher total content of tannins and G. parviflora presented higher amount of phenols and flavonoids. Chemical characterization showed the presence of flavonoids, phenolic acids and sesquiterpene lactones in V. polyanthes extract, and steroids, phenolic acids and fatty acids (Poly Unsaturated Fatty Acids - PUFA) in G. parviflora extract. V. polyanthes extract stood out in the Fe2+ chelation test. G. parviflora extract did not present outstanding antioxidant results in the tested protocols. Both species showed a tendency to promote cytotoxicity in human lymphocyte cells. Regarding the antiproliferative effect, both species were able to reduce S-180 cell viability and G. parviflora extract showed high antiproliferative potential in the assay with AGS cells. These findings reinforce the medicinal use of these plants, as well as suggest their potential use for the development of new drugs and for the treatment of cancers.

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Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

Current Analytical Chemistry ◽

10.2174/1573411016999200729111951 ◽

2020 ◽

Vol 16 ◽

Author(s):

Natasa P. Kalogiouri ◽

Natalia Manousi ◽

Erwin Rosenberg ◽

George A. Zachariadis ◽

Victoria F. Samanidou

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

High Performance ◽

Unsaturated Fatty Acids ◽

Nutritional Deficiencies ◽

Analytical Technique ◽

High Volatility ◽

Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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Phytochemicals and Biological Activity of Desert Date (Balanites aegyptiaca (L.) Delile)

Plants ◽

10.3390/plants10010032 ◽

2020 ◽

Vol 10 (1) ◽

pp. 32

Author(s):

Hosakatte Niranjana Murthy ◽

Guggalada Govardhana Yadav ◽

Yaser Hassan Dewir ◽

Abdullah Ibrahim

Keyword(s):

Biological Activity ◽

Middle East ◽

Bioactive Compounds ◽

Phenolic Acids ◽

Tree Species ◽

Indian Subcontinent ◽

Therapeutic Agents ◽

Root Bark ◽

Balanites Aegyptiaca ◽

Anti Inflammatory

Many underutilized tree species are good sources of food, fodder and possible therapeutic agents. Balanites aegyptiaca (L.) Delile belongs to the Zygophyllaceae family and is popularly known as “desert date”, reflecting its edible fruits. This tree grows naturally in Africa, the Middle East and the Indian subcontinent. Local inhabitants use fruits, leaves, roots, stem and root bark of the species for the treatment of various ailments. Several research studies demonstrate that extracts and phytochemicals isolated from desert date display antioxidant, anticancer, antidiabetic, anti-inflammatory, antimicrobial, hepatoprotective and molluscicidal activities. Mesocarp of fruits, seeds, leaves, stem and root bark are rich sources of saponins. These tissues are also rich in phenolic acids, flavonoids, coumarins, alkaloids and polysterols. Some constituents show antioxidant, anticancer and antidiabetic properties. The objective of this review is to summarize studies on diverse bioactive compounds and the beneficial properties of B. aegyptiaca.

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Bee collected pollen as a value-added product rich in bioactive compounds and unsaturated fatty acids: A comparative study from Turkey and Romania

LWT ◽

10.1016/j.lwt.2021.111925 ◽

2021 ◽

pp. 111925

Author(s):

Rodica Mărgăoan ◽

Aslı Özkök ◽

Şaban Keskin ◽

Nazlı Mayda ◽

Adriana Cristina Urcan ◽

...

Keyword(s):

Fatty Acids ◽

Comparative Study ◽

Bioactive Compounds ◽

Unsaturated Fatty Acids ◽

Value Added ◽

A Value

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Development and Validation of RP-HPLC Method for Vicenin-2, Orientin, Cynaroside, Betulinic Acid, Genistein, and Major 8 Bioactive Constituents with LC-ESI-MS/MS profiling in Ocimum genus

Journal of AOAC International ◽

10.1093/jaoacint/qsab067 ◽

2021 ◽

Author(s):

Aboli Girme ◽

Prajkta Bhoj ◽

Ganesh Saste ◽

Sandeep Pawar ◽

Amit Mirgal ◽

...

Keyword(s):

Bioactive Compounds ◽

Betulinic Acid ◽

Gradient Elution ◽

Carnosic Acid ◽

Bioactive Constituents ◽

Validation Data ◽

Method Performance ◽

Esi Ms ◽

Stress Relieving ◽

Quality Control Tool

Abstract Background Ocimum genus known as Tulsi or Basil is a prominent botanical class in Asian culture, especially in India. The leaves have an immunomodulatory, antioxidant, stress-relieving, and adaptogenic role in traditional and modern medicine, with prominent usage in herbal teas and nutraceuticals. Objective The HPLC-PDA method was developed and validated for vicenin-2, orientin, cynaroside, betulinic acid, genistein, with syringic acid, rosmarinic acid, eugenol, carnosic acid, oleanolic acid, ursolic acid, luteolin, apigenin for quantification and confirmed using a novel ESI -MS/MS method in the Ocimum samples. Method The methodology parameters were developed on the RP-C18 column with a gradient elution of 1 mL/min flow rate for 0.1% o-phosphoric acid and acetonitrile at 210 and 340 nm wavelengths. Result The validation data for 13 bioactive compounds showed good linearity (r2> 0.99) with sensitive LOD (0.034-0.684 µg/mL) and LOQ (0.100-2.068 µg/mL) with recoveries (83.66-101.53%).The results were found precise (RSD,<5.0%) and accurate (RE, -0.60-1.06) for the quantification. The method performance was verified and found robust by analyzing ten samples of O. tenuiflorum from the ten geographical states of India (RSD, < 5.0%). Conclusion The validated HPLC-PDA method was found selective and suitable for analyzing thirteen compounds in O. tenuiflorum and twelve cultivars from the Ocimum genus as a quality control tool. This method can be used in routine analysis as an inexpensive alternative to advanced techniques. Highlights This work is the first to report for vicenin-2, orientin, cynaroside, betulinic acid, and genistein, with simultaneous analysis of eight bioactive compounds in the Ocimum genus.

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