scholarly journals Potential Use of Colored LED Lights to Increase the Production of Bioactive Metabolites Hedyotis corymbosa (L.) Lam

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 225
Author(s):  
Anh Tuan Le ◽  
Ju-Kyung Yu ◽  
Gyung-Deok Han ◽  
Thuong Kiet Do ◽  
Yong-Suk Chung

Hedyotis corymbosa (L.) Lam is a wild herb that is used in traditional Indian, Chinese, and African medicine. Light-emitting diode (LED) technology is paving the way to enhance crop production and inducing targeted photomorphogenic, biochemical, or physiological responses in plants. This study examines the efficiency of H. corymbosa (L.) Lam production under blue 450 nm and red 660 nm LED lights for overall plant growth, photosynthetic characteristics, and the contents of metabolite compounds. Our research showed that blue LED lights provided a positive effect on enhancing plant growth and overall biomass. In addition, blue LED lights are more effective in controlling the production of sucrose, starch, total phenolic compounds, and total flavonoid compared to red LED lights. However, blue and red LED lights played essential but different roles in photosynthetic characteristics. Our results showed the potential of colored LED light applications in improving farming methods and increasing metabolite production in herbs. LED lights are safer alternatives than genetically modified organisms or genome editing.

2016 ◽  
Vol 8 (2) ◽  
pp. 156-160 ◽  
Author(s):  
Syeda Jabun NAHAR ◽  
Syed M. HAQUE ◽  
Shimasaki KAZUHIKO

The aim of this study was to present chondroitin sulfate as a plant growth regulator and to give an overview about light effects on PLBs (protocorm like bodies) culture of Cymbidium dayanum and Cymbidium finlaysonianum cultured in vitro. Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars N-acetylgalactosamine and glucuronic acid. It is widely used as a material for food ingredients, cosmetics and medicine. PLBs were cultured on modified MS medium containing different concentration of chondroitin sulfate (0, 0.1, 1 and 10 mg/l), under four sources of lights: conventional white fluorescent tube, red LED, green LED and blue LED. In C. dayanum, 100% PLBs formation rate was observed at 0.1 mg/l chondroitin sulfate with modified MS medium under green LED and 1 mg/l chondroitin sulfate under blue LED; the maximum shoots and roots formation were observed under green LEDs (93% and 80% respectively) when media contained 0.1 mg/l chondroitin sulfate. In C. finlaysonianum, every concentrations of chondroitin sulfate enhanced the growth rate of PLBs when compared to control treatment, under all four sources of lights. The highest values were recorded with 0.1 mg/l chondroitin sulfate which induced 100% PLBs formation under blue LED, while 10 mg/l chondroitin sulfate had induced 100% PLBs formation under green LED. The highest percentage of shoots (73%) was initiated in the medium containing 10 mg/l chondroitin sulfate under green LED. Plant development was strongly influenced by the light quality and plant growth regulator functions as chemical messengers for intercellular communication of plant. The results demonstrated that low concentrations of chondroitin sulfate could promote PLBs, shoots and roots formation of Cymbidium spp. under green and blue LED.


Author(s):  
Doan Phan Phuong Thao ◽  
Tuan Anh Le ◽  
Phan Ngo Hoang

Polygonum cuspidatum Sieb. et Zucc, a medicinal plant, contains many phenolic compounds such as resveratrol. It has antioxidant, antibacterial, anti-cancer, HIV, and neuron protective properties. In this study, stem segments having an auxiliary bud were cultured in vitro on MS medium supplemented with TDZ at 0.1 mg/L for obtaining the highest number of shoots (8–9 shoots/cluster after eight weeks). The bud shoots were originated from the differentiation in the periphery of the lateral meristem. After eight weeks, the shoots cultured under the monochromatic light showed that the plant height, fresh and dry weight was higher than those grown under the fluorescent light at the same intensity of 50 µmol photon/m2/sec. Blue LED reduced the number of shoots/cluster, and respiration intensity of the inoculum compared to the red LED or fluorescent light conditions. However, after eight weeks, the total sugar and phenolic content in leaves and stems, as well as the resveratrol content of shoots under blue light, were always higher than under red LED or fluorescent light. Moreover, when samples were exposed under the one-week blue light condition at a double intensity (100 µmol photon/m2/sec), the shoot clusters growing under blue LED also had a remarkably high total phenolic content and significantly high resveratrol levels compared to the two other treatments. The roles of endogenous growth regulators in shoot clusters under different lighting conditions were also analyzed and discussed.


2019 ◽  
Vol 12 (7) ◽  
pp. 1052-1059 ◽  
Author(s):  
Essam S. Soliman ◽  
Rania A. Hassan

Background and Aim: Manipulating lighting colors and regimens is considered an effective mean for improving broiler productivity. The influence of red, blue, and white light-emitting diode (LED) was investigated using three different regimens of lighting and darkness; continuous 23 h light (L):1 h dark (D), continuous 18 h L:6 h D, and intermittent 16 h L:8 h D hours on the performance, carcass weight (CW), feed and water intake (WI), serum glucose (GLUCO), triglycerides (TG), and cholesterol (TC), intestinal bacterial load, growth and metabolic hormones, and efficiency of Newcastle disease (ND) vaccine. Materials and Methods: A total of 252 1-day-old Ross broilers on deep litter were divided into nine groups. The 1st, 4th, and 7th groups were exposed to continuous 23L:1D, the 2nd, 5th, and 8th groups were exposed to continuous 18L:6D, and the 3rd, 6th, and 9th groups were exposed to intermittent 16L:8D (4L:2D, 4 times) lighting regimen using red, blue, and white LED lights, respectively. A total of 1350 samples (225 sera, 225 swabs, and 900 organ samples) were collected. Results: Blue LED group revealed a highly significant increase (p<0.01) in live body weight, body weight gain, performance index, CW, spleen, heart, and liver weights, and anti-ND antibody titer, as well as a highly significant decline (p<0.01) of feed intake, WI, GLUCO, TG, TC, growth hormone, insulin, tri-iodothyronine (T3), tetra-iodothyronine (T4), total bacterial count (TBC), and total Enterobacteriaceae count compared to red and white LED lights in all tested lighting regimens. Continuous 23L:1D and 18L:6D regimens were significantly (p<0.01) superior to intermittent 16L:8D in their influence on the performance, CW, biochemistry, hormonal profile, and bacterial load. Conclusion: The blue LED light associated with continuous 18L:6D or 23L:1D h regimen is highly recommended in broiler houses for their enhancing the productive performance, growth, and immunity.


Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 359 ◽  
Author(s):  
Gludia M. Maroga ◽  
Puffy Soundy ◽  
Dharini Sivakumar

The influence of emitting diode (LED) treatments for 8 h per day on functional quality of three types of fresh-cut sweet peppers (yellow, red, and green) were investigated after 3, 7, 11, and 14 days postharvest storage on the market shelf at 7 °C. Red LED light (660 nm, 150 μmol m−2 s−1) reduced weight loss to commercially acceptable level levels (≤2.0%) in fresh-cuts of yellow and green sweet peppers at 7 and 11 d, respectively. Blue LED light (450 nm, 100 μmol m−2 s−1) maintained weight loss acceptable for marketing in red fresh-cut sweet peppers up to 11 d. Highest marketability with minimum changes in color difference (∆E) and functional compounds (total phenols, ascorbic acid content, and antioxidant activity) were obtained in yellow and green sweet pepper fresh-cuts exposed to red LED light up to 7 and 11 d, respectively, and for red sweet pepper fresh-cuts exposed to blue LED light for 11 d. Red LED light maintained the highest concentrations of β carotene, chlorophyll, and lycopene in yellow, green, and red sweet pepper fresh-cuts up to 7 d. Similarly, blue LED light showed the highest increase in lycopene concentrations for red sweet pepper fresh-cuts up to 7 d. Red LED (yellow and green sweet peppers) and blue LED (red sweet pepper) lights maintained phenolic compounds by increasing phenylalanine ammonia lyase activity. Thus, the results indicate a new approach to improve functional compounds of different types of fresh-cut sweet pepper.


2020 ◽  
Vol 47 (9) ◽  
pp. 853 ◽  
Author(s):  
Zahra Mirzahosseini ◽  
Leila Shabani ◽  
Mohammad R. Sabzalian

A comparison among four light emitting diode (LED) lights including red LED (R), blue LED (B), red (70%) + blue (30%) LED (RB) and white LED (W) as well as fluorescent (F) light was made on antioxidative capacity of Arabidopsis thaliana (L.) Heynh. in response to wounding. Under wound-stress condition, LED-exposed plants, especially RB-irradiated plants, maintained significantly higher shoot dry weight and antioxidant enzymes activities compared with those irradiated with fluorescent lights. The highest amounts of both chlorophyll a and b were observed in the leaves treated with B light. Also, the concentration of H2O2 was higher under the condition of RB and B lights compared with the other light environments. The highest amount of malondialdehyde was measured in plants exposed to F and B lights. Similarly, wounded leaves under F and B light conditions showed the maximum lipoxygenase activity, whereas R-exposed leaves had the lowest lipoxygenase activity. In contrast, the highest level of phenolic compounds was found in R and RB exposed leaves in response to wounding. Among the five light treatments, RB and B lights were more effective in stimulating anthocyanin synthesis; however, RB-exposed plants were more efficient in the late-induction of the PAL gene (phenylalanine ammonia lyase catalyses the first step of the general phenylpropanoid pathway). Collectively, we reasoned that RB light condition gives a superior capacity to Arabidopsis thaliana to tolerate wound-stress. Also, we propose the probable signalling role of ROS in light-stimulated wound responses in Arabidopsis.


2022 ◽  
Vol 21 (1) ◽  
pp. 11-21
Author(s):  
Bambang Kusmayadi Gunawan ◽  
Kukuh Nirmala ◽  
Dinar Tri Soelistyowati ◽  
Daniel Djokosetiyanto ◽  
Wildan Nurussalam

This study aimed to evaluate the effect of light spectrum on growth and color performance of giant giant gourami Padang strain. The experiment used a completely randomized design (RAL) with four light emitting diode (LED) treatments in  different emission spectra (white, red, green, and blue) at 550 Lux intensity, compared to the control treatment (light room with white tubular lamp at 50 Lux intensity). The irradiation was carried out for 12 hours of photoperiod. The fish used had the total length of 82.90±4.2 mm and body weight of 9.87 ± 0.99 g. The highest growth performance was found in blue LED treatment with the specific growth rate of  2.73 ± 0.2% and feed efficiency of 86.26 ± 2.71%. The best color performance was found in red LED treatment with the RGB ratio of 44.57 ± 0.62% in dorsal fin, 38.41 ± 1.36% in pectoral fins, and 45.33 ± 2.25% in anal fin with the chromatophore cell concentration at 1.973±58 cells/mm2.   Keywords : Osphronemus gouramy, blue LED, spectrum, chromatophore, light   ABSTRAK   Penelitian ini bertujuan untuk mengevaluasi pengaruh spektrum cahaya terhadap kinerja pertumbuhan dan warna ikan gurami strain Padang. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan cahaya lampu light emitting diodes (LED)  yang memiliki spektrum panjang gelombang berbeda (putih, merah, hijau dan biru) intensitas 550 Lux dan kontrol (cahaya ruang berasal dari lampu tubular putih intensitas 50 Lux). Penyinaran dilakukan selama 12 jam mengikuti fotoperiod. Ikan uji yang digunakan memiliki panjang total 82,90 ± 4,2 mm, dengan bobot 9,87 ± 0,99 g. Kinerja pertumbuhan terbaik terdapat pada perlakuan LED biru dengan laju pertumbuhan spesifik sebesar 2,73 ± 0,2% dan efisiensi pakan sebesar 86,26 ± 2,71%. Performa warna terbaik terdapat pada perlakuan LED merah dengan rasio warna merah pada RGB bagian dorsal sebesar 44,57 ± 0,62%, sirip pektoral sebesar 38,41 ± 1,36%, dan sirip anal sebesar 45,33 ±  2,25% dengan jumlah sel kromatofor sebanyak 1973 sel/mm2.   Kata kunci : Osphronemus gouramy, LED biru, spektrum, kromatofor, cahaya        


2018 ◽  
Vol 2 (1) ◽  
pp. 79 ◽  
Author(s):  
Ardiansyah Azhary Suhandoko ◽  
Sumarsono Sumarsono ◽  
Endang Dwi Purbajanti

This research has an aim to observe the production of lettuce (Lactuca sativa L.) on hydroponic technology of floating system with red and blue LED (Light Emitting Diode) at night. This research was conducted inside the Screenhouse in which located on the top of Griya Kads House building, Bulusan Village, Tembalang Sub-district from May to June 2017. The research was conducted by 3x3 factorial experiments in Nested Design with 3 replications. The first factor was 0, 1.5 and 3 hours irradiation of red and blue LED lights at night. The second factor was 3, 5 and 7 ml/liter of AB mix nutrient concentration which was nested in the first factor. The data collected were fresh weight, dry weight and root canopy ratio. The results of the study showed that the treatment of AB mix nutrient concentration had no significant effect on all parameters. The production of lettuce with level of 5 ml concentration of AB mix was quite good, yet 3 ml and 7 ml levels didn’t improve lettuce production. The duration of red and blue LED irradiation only resulted in an increase in the production of canopy fresh weight. This research can be concluded that 3 hours was sufficient to give the good results to improve the results of lettuce plants production. Keywords : Lettuce Production, AB Mix Nutrition, Red and Blue LED.


HortScience ◽  
2015 ◽  
Vol 50 (3) ◽  
pp. 351-357 ◽  
Author(s):  
Ricardo Hernández ◽  
Chieri Kubota

To increase the available photosynthetic photon flux (PPF) for plant growth, greenhouse growers sometimes use electric lighting to supplement solar light. The conventional lighting technology used to increase PPF in the greenhouse is high-pressure sodium lamps (HPS). A potential alternative to HPS is high-intensity light-emitting diodes (LEDs). The objective of this study is to compare supplemental LED lighting with supplemental HPS lighting in terms of plant growth and morphology as well as discuss the energy use efficiencies of the fixtures. There were three light treatments: 1) blue LED (peak wavelength 443 nm); 2) red LED (peak wavelength 633 nm); and 3) HPS, to provide 3.7 ± 0.2 mol·m−2·d−1 (background solar radiation of 6.3 ± 0.9 mol·m−2·d−1). Cucumber (Cucumis sativus) plants at the transplanting stage (26 to 37 days) under HPS had 28% greater dry mass than did plants under the LED treatments. This can be attributed to the higher leaf temperature under the HPS treatment. No differences were observed in growth parameters (dry mass, fresh weight, or number of leaves) between the blue and red LED treatments. Plants under the blue LED treatment had greater net photosynthetic rate and stomatal conductance (gS) than those under the red LED and HPS treatments. Plants under the blue LED and HPS treatments had 46% and 61% greater hypocotyl length than those under the red LED, respectively. The fixture PPF efficiencies used in the experiment were 1.9, 1.7, and 1.64 μmol·J−1 for the blue LED, red LED, and HPS treatments, respectively; however, the fixture growing efficiency (g·kWh−1) of HPS was 6% and 17% greater than the blue LED and red LED treatment, respectively. In summary, supplemental red LED produced desirable plant compactness and HPS had greater fixture growing efficiency than LEDs.


2021 ◽  
Vol 8 (1) ◽  
pp. 86-99
Author(s):  
Jun Jiang ◽  
◽  
Mehrdad Moallem ◽  
Youbin Zhengi ◽  
◽  
...  

In this paper, an intelligent lighting instrumentation and automation system is presented with the objective of achieving high energy-efficiency in greenhouse supplemental lighting based on the Internet of Things (IoT) technology. The system runs on a Raspbian operating system which interacts with wireless-enabled light emitting diode (LED) fixtures for plant growth, an online data server, and different light sensors including RGB and quantum sensors. The communication is achieved through RestFul API, UART, and I2C. The system is utilized to implement a feedback controller that automatically adjusts the light dimming levels and, in particular, the ratio of red and blue light intensities based on the plants’ needs. A series of experiments involving plant growth were conducted which indicate that the proposed system can achieve energy-savings up to 34%, when compared to a conventional time scheduling scheme. Additionally, the experiments demonstrate that the system can achieve a highly uniform light distribution under unpredictable natural lighting conditions while saving energy due to supplemental lighting.


2019 ◽  
Vol 6 (1) ◽  
pp. 16
Author(s):  
Musdarina Musdarina ◽  
Hernawati Hernawati ◽  
Fitriyanti Fitriyanti

Research has been conducted which aims to determine the effect of various colors of LED light and sound effects with frequency of 280Hz-16KHz. This research was conducted by seeding mustard greens for 2 weeks then moving mustard greens to polybags by giving two treatments. First treatment of mustard greens illuminated by LED lights with the color red, yellow, green and the fourth polybag left without the addition of LED light. The second treatment is every 1 polybag of green mustard illuminated by LED lights with red, yellow, green lights at night for 4 hours then exposed to sound in the morning for 4 hours and the fourth polybag is exposed to sound without the addition of lights. The results of this study indicate the effect of the addition of red LED light color on the plant is clearly seen in the width of green mustard leaves that have wider leaves between the addition of other LED light colors and the number of leaves produced more than the addition of yellow, green and without additional light. The effect of exposure to sound on the plant has a fairly good influence on the height of the stem, stem width and number of leaves compared to plants that are not given sound exposure and in plants that are given additional LED light then the sound produces better plant growth compared to plants that only given additional lights or just given a sound.


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