scholarly journals The Effect of Agarose on 3D Bioprinting

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 4028
Author(s):  
Chi Gong ◽  
Zhiyuan Kong ◽  
Xiaohong Wang
Keyword(s):  
Mechanical Properties ◽  
Low Temperature ◽  
Internal Structure ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Two Temperatures ◽  
Formed Structure ◽  
Accuracy And Stability ◽  
Physical Crosslinking ◽  
Complex Organ

In three-dimensional (3D) bioprinting, the accuracy, stability, and mechanical properties of the formed structure are very important to the overall composition and internal structure of the complex organ. In traditional 3D bioprinting, low-temperature gelatinization of gelatin is often used to construct complex tissues and organs. However, the hydrosol relies too much on the concentration of gelatin and has limited formation accuracy and stability. In this study, we take advantage of the physical crosslinking of agarose at 35–40 °C to replace the single pregelatinization effect of gelatin in 3D bioprinting, and printing composite gelatin/alginate/agarose hydrogels at two temperatures, i.e., 10 °C and 24 °C, respectively. After in-depth research, we find that the structures manufactured by the pregelatinization method of agarose are significantly more accurate, more stable, and harder than those pregelatined by gelatin. We believe that this research holds the potential to be widely used in the future organ manufacturing fields with high structural accuracy and stability.

Experimental Study on Mechanical Properties of Rubber Used for Damping Bearing

2011 ◽  
Vol 189-193 ◽  
pp. 1132-1136 ◽  
Author(s):  
Yong Xu Zhao ◽  
Wen Jun Hu ◽  
Jun Mei ◽  
Niu Wei ◽  
Jian Jun Xie
Keyword(s):  
Mechanical Properties ◽  
Low Temperature ◽  
Three Dimensional ◽  
Middle Part ◽  
Loading Conditions ◽  
Deflection Curve ◽  
Rubber Bearing ◽  
Components Analysis ◽  
The Impact ◽  
Load Deflection Curve

After testing on T-type rubber bearing under tensile, compression and shear mechanical properties under different temperature in this paper. Obtained load deflection curve and destructive mode under different loading conditions at -40 and normal temperature of rubber components. Analysis the impact of temperature and the loading conditions that effect on load-elongation and destructive mode of T-type damping rubber structure. It showed that T-end rubber bearing has different kinds of deformation under different force-giving methods. Under compression, the stress pattern of the rubber bearing is three-dimensional and middle rubber bear the greatest force. Under tensile loading, the middle part of the rubber contract and the side with smaller lateral section has greater shrinkage; moreover, damage occurred in the area with stress concentration and weak strength. Under shearing action, extrude faces appeared with crinkle and damage occurred in the middle part of extrude faces. At the low temperature-40 , rubber support still has great elastic properties. The low temperature has a big effect on tensile properties and has little effect on damage properties.

scholarly journals Chitosans for Tissue Repair and Organ Three-Dimensional (3D) Bioprinting

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 765 ◽  
Author(s):  
Shenglong Li ◽  
Xiaohong Tian ◽  
Jun Fan ◽  
Hao Tong ◽  
Qiang Ao ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Biomedical Applications ◽  
Large Scale ◽  
Scale Up ◽  
Three Dimensional ◽  
The Body ◽  
3D Bioprinting ◽  
Chemically Modified ◽  
Excellent Candidate ◽  
Complex Organ

Chitosan is a unique natural resourced polysaccharide derived from chitin with special biocompatibility, biodegradability, and antimicrobial activity. During the past three decades, chitosan has gradually become an excellent candidate for various biomedical applications with prominent characteristics. Chitosan molecules can be chemically modified, adapting to all kinds of cells in the body, and endowed with specific biochemical and physiological functions. In this review, the intrinsic/extrinsic properties of chitosan molecules in skin, bone, cartilage, liver tissue repair, and organ three-dimensional (3D) bioprinting have been outlined. Several successful models for large scale-up vascularized and innervated organ 3D bioprinting have been demonstrated. Challenges and perspectives in future complex organ 3D bioprinting areas have been analyzed.

scholarly journals PLGA/COL I Composite Scaffold Printed by a Low-temperature Deposition Manufacturing Technique for Application to Cartilage Tissue Engineering

2020 ◽  
Author(s):  
Liangquan Peng ◽  
Yong He ◽  
Weimin Zhu ◽  
Wei Lu ◽  
Yong Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Low Temperature ◽  
Composite Scaffold ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Composite Scaffolds ◽  
Low Temperature Deposition ◽  
Temperature Deposition

Abstract Background Composite scaffolds of poly(lactic-co-glycolic acid) (PLGA) and PLGA/COL I were developed by a low-temperature deposition manufacturing (LDM) technique using three-dimensional printing technology. Their physical properties were tested, and the scaffolds were then used as cell culture platforms to prepare an ideal scaffold for cartilage tissue engineering. Methods The LDM technique was used to fabricate PLGA and PLGA/COL I composite scaffolds. The macrostructure, micromorphology, porosity, hydrophobicity, mechanical properties, and chemical structure of these scaffolds were examined. Primary chondrocytes were isolated and identified, second-passage cells were seeded onto the two scaffolds, and the adhesion and proliferation of the cells were determined. Results Both the PLGA and PLGA/COL I scaffolds prepared by LDM displayed a regular three-dimensional structure with high porosity. The PLGA scaffold had better mechanical properties than the PLGA/COL I scaffold, while the latter had significantly higher hydrophilicity than the former. The PLGA/COL I scaffold cultured with chondrocytes exhibited a higher adhesion rate and proliferation rate than the PLGA/COL I scaffold. Conclusion The novel PLGA/COL I composite scaffold printed by the LDM technique exhibited favourable biocompatibility and biomechanical characteristics and could be a good candidate for cartilage tissue engineering.

scholarly journals Influence of Crosslinking on the Mechanical Behavior of 3D Printed Alginate Scaffolds: Experimental and Numerical Approaches

2018 ◽  
Author(s):  
Saman Naghieh ◽  
Mohammad Reza Karamooz-Ravari ◽  
Md Sarker ◽  
Eva Karki ◽  
Xiongbiao Chen
Keyword(s):  
Mechanical Properties ◽  
Elastic Modulus ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Decisive Role ◽  
Element Model ◽  
Tissue Scaffolds ◽  
3D Bioprinting ◽  
3D Printed ◽  
Numerical Approaches

Tissue scaffolds fabricated by three-dimensional (3D) bioprinting are attracting considerableattention for tissue engineering applications. Because the mechanical properties of hydrogelscaffolds should match the damaged tissue, changing various parameters during 3D bioprintinghas been studied to manipulate the mechanical behavior of the resulting scaffolds. Crosslinkingscaffolds using a cation solution (such as CaCl2) is also important for regulating the mechanicalproperties, but has not been well documented in the literature. Here, the effect of variedcrosslinking agent volume and crosslinking time on the mechanical behavior of 3D bioplottedalginate scaffolds was evaulated using both experimental and numerical methods. Compressiontests were used to measure the elastic modulus of each scaffold, then a finite element model wasdeveloped and a power model used to predict scaffold mechanical behavior. Results showed thatcrosslinking time and volume of crosslinker both play a decisive role in modulating the mechanicalproperties of 3D bioplotted scaffolds. Because mechanical properties of scaffolds can affect cellresponse, the findings of this study can be implemented to modulate the elastic modulus ofscaffolds according to the intended application.

scholarly journals Cellulose Nanocrystal-Enhanced Thermal-Sensitive Hydrogels of Block Copolymers for 3D Bioprinting

2021 ◽  
Vol 7 (4) ◽  
pp. 397
Author(s):  
Yuecheng Cui ◽  
Ronghua Jin ◽  
Yifan Zhang ◽  
Meirong Yu ◽  
Yang Zhou ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Block Copolymers ◽  
3D Printing ◽  
Three Dimensional ◽  
Enhancement Effect ◽  
3D Bioprinting ◽  
Forming Mechanism ◽  
Molecular Weights ◽  
Good Biocompatibility ◽  
The Stability

 The hydrogel formed by polyethylene glycol-aliphatic polyester block copolymers is an ideal bioink and biomaterial ink for three-dimensional (3D) bioprinting because of its unique temperature sensitivity, mild gelation process, good biocompatibility, and biodegradability. However, the gel forming mechanism based only on hydrophilic-hydrophobic interaction renders the stability and mechanical strength of the formed hydrogels insufficient, and cannot meet the requirements of extrusion 3D printing. In this study, cellulose nanocrystals (CNC), which is a kind of rigid, hydrophilic, and biocompatible nanomaterial, were introduced to enhance the hydrogels so as to meet the requirements of extrusion 3D printing. First, a series of poly(ε-caprolactone/lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone/ lactide) (PCLA-PEG-PCLA) triblock copolymers with different molecular weights were prepared. The thermodynamic and rheological properties of CNC-enhanced hydrogels were investigated. The results showed that the addition of CNC significantly improved the thermal stability and mechanical properties of the hydrogels, and within a certain range, the enhancement effect was directly proportional to the concentration of CNC. More importantly, the PCLA-PEG-PCLA hydrogels enhanced by CNC could be extruded and printed through temperature regulation. The printed objects had high resolution and fidelity with effectively maintained structure. Moreover, the hydrogels have good biocompatibility with a high cell viability. Therefore, this is a simple and effective strategy. The addition of the hydrophilic rigid nanoparticles such as CNC improves the mechanical properties of the soft hydrogels which made it able to meet the requirements of 3D bioprinting.

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Biotemplated facile synthesis of three‐dimensional micro/nanoporous tin oxide: improving the flammable and mechanical properties of flexible PVC

2019 ◽  
Vol 14 (8) ◽  
pp. 828-830 ◽  
Author(s):  
Weihua Meng ◽  
Weihong Wu ◽  
Weiwei Zhang ◽  
Luyao Cheng ◽  
Yunhong Jiao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tin Oxide ◽  
Three Dimensional ◽  
Facile Synthesis

scholarly journals Rapid Growth of TiO2 Nanoflowers via Low-Temperature Solution Process: Photovoltaic and Sensing Applications

Materials ◽  
2019 ◽  
Vol 12 (4) ◽  
pp. 566 ◽  
Author(s):  
M. Akhtar ◽  
Ahmad Umar ◽  
Swati Sood ◽  
InSung Jung ◽  
H. Hegazy ◽  
...  
Keyword(s):  
Low Temperature ◽  
Anatase Phase ◽  
Three Dimensional ◽  
Solution Process ◽  
Dye Sensitized Solar Cell ◽  
Sensor Applications ◽  
Dye Sensitized ◽  
Sensing Applications ◽  
Density Growth ◽  
Temperature Solution

This paper reports the rapid synthesis, characterization, and photovoltaic and sensing applications of TiO2 nanoflowers prepared by a facile low-temperature solution process. The morphological characterizations clearly reveal the high-density growth of a three-dimensional flower-shaped structure composed of small petal-like rods. The detailed properties confirmed that the synthesized nanoflowers exhibited high crystallinity with anatase phase and possessed an energy bandgap of 3.2 eV. The synthesized TiO2 nanoflowers were utilized as photo-anode and electron-mediating materials to fabricate dye-sensitized solar cell (DSSC) and liquid nitroaniline sensor applications. The fabricated DSSC demonstrated a moderate conversion efficiency of ~3.64% with a maximum incident photon to current efficiency (IPCE) of ~41% at 540 nm. The fabricated liquid nitroaniline sensor demonstrated a good sensitivity of ~268.9 μA mM−1 cm−2 with a low detection limit of 1.05 mM in a short response time of 10 s.

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

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