scholarly journals High Retention and Purification of Bromelain Enzyme (Ananas comosus L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 264
Author(s):  
Felix M. Carbajal Gamarra ◽  
José C. C. Santana ◽  
Segundo A. V. Llanos ◽  
Jorge A. Heredia Pérez ◽  
Fábio Richard Flausino ◽  
...  
Keyword(s):  
Human Health ◽  
High Efficiency ◽  
Membrane Separation ◽  
Separation Process ◽  
Polymeric Membranes ◽  
Operating Conditions ◽  
Ananas Comosus ◽  
Pineapple Juice ◽  
The World ◽  
Pineapple Fruit

The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltration from pineapple juice (Ananas comusus L. Merrill). All assays were carried out at pH 7.0 and 7.5, and at 0.05 and 0.40 bar of transmembrane pressures. Results have shown that at the best operating conditions, between 85 and 87% of bromelain activity was recovered using the plain membrane separation process at 0.05 bar. The ultrafiltration has shown the capacity to retain 100% of proteolytic activity of the bromelain extracted. The samples have kept the same physics properties after ultrafiltration, and the result was verified via electrophoresis. The bromelain enzyme obtained was characterized, and pH 7 and between 30 and 40 °C were the best conditions. Therefore, this work shows that the use of both polymeric membranes has shown high efficiency, and can be used in the purification of bromelain enzymes.

scholarly journals Influence of Casting Solvents on CO2/CH4 Separation Using Polysulfone Membranes

Membranes ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 286
Author(s):  
Roba M. Almuhtaseb ◽  
Ahmed Awadallah-F ◽  
Shaheen A. Al-Muhtaseb ◽  
Majeda Khraisheh
Keyword(s):  
High Efficiency ◽  
In Situ Synthesis ◽  
Separation Process ◽  
Polymeric Membranes ◽  
Gas Mixture ◽  
Manufacturing Cost ◽  
Binary Gas Mixture ◽  
Maximum Permeability ◽  
Polysulfone Membranes

Polysulfone membranes exhibit resistance to high temperature with low manufacturing cost and high efficiency in the separation process. The composition of gases is an important step that estimates the efficiency of separation in membranes. As membrane types are currently becoming in demand for CO2/CH4 segregation, polysulfone will be an advantageous alternative to have in further studies. Therefore, research is undertaken in this study to evaluate two solvents: chloroform (CF) and tetrahydrofuran (THF). These solvents are tested for casting polymeric membranes from polysulfone (PSF) to separate every single component from a binary gas mixture of CO2/CH4. In addition, the effect of gas pressure was conducted from 1 to 10 bar on the behavior of the permeability and selectivity. The results refer to the fact that the maximum permeability of CO2 and CH4 for THF is 62.32 and 2.06 barrer at 1 and 2 bars, respectively. Further, the maximum permeability of CF is 57.59 and 2.12 barrer at 1 and 2 bars, respectively. The outcome selectivity values are 48 and 36 for THF and CF at 1 bar, accordingly. Furthermore, the study declares that with the increase in pressure, the permeability and selectivity values drop for CF and THF. The performance for polysulfone (PSF) membrane that is manufactured with THF is superior to that of CF relative to the Robeson upper bound. Therefore, through the results, it can be deduced that the solvent during in-situ synthesis has a significant influence on the gas separation of a binary mixture of CO2/CH4.

Membrane-based separation technologies: from polymeric materials to novel process: an outlook from China

2019 ◽  
Vol 36 (1) ◽  
pp. 67-105 ◽  
Author(s):  
Zhongyi Jiang ◽  
Liangyin Chu ◽  
Xuemei Wu ◽  
Zhi Wang ◽  
Xiaobin Jiang ◽  
...  
Keyword(s):  
Gas Separation ◽  
Process Design ◽  
Chemical Engineering ◽  
High Efficiency ◽  
Process Development ◽  
Membrane Separation ◽  
Process Integration ◽  
Polymeric Materials ◽  
Separation Process ◽  
Future Research

Abstract During the past two decades, research on membrane and membrane-based separation process has developed rapidly in water treatment, gas separation, biomedicine, biotechnology, chemical manufacturing and separation process integration. In China, remarkable progresses on membrane preparation, process development and industrial application have been made with the burgeoning of the domestic economy. This review highlights the recent development of advanced membranes in China, such as smart membranes for molecular-recognizable separation, ion exchange membrane for chemical productions, antifouling membrane for liquid separation, high-performance gas separation membranes and the high-efficiency hybrid membrane separation process design, etc. Additionally, the applications of advanced membranes, relevant devices and process design strategy in chemical engineering related fields are discussed in detail. Finally, perspectives on the future research directions, key challenges and issues in membrane separation are concluded.

scholarly journals Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp

2009 ◽  
Vol 52 (2) ◽  
pp. 457-464 ◽  
Author(s):  
Francisco Luiz Gumes Lopes ◽  
João Baptista Severo Júnior ◽  
Roberto Rodrigues de Souza ◽  
Daniela Diniz Ehrhardt ◽  
José Carlos Curvelo Santana ◽  
...  
Keyword(s):  
Proteolytic Activity ◽  
Membrane Separation ◽  
Ananas Comosus ◽  
Transmembrane Pressure ◽  
Operation Condition ◽  
Separation Processes ◽  
Pineapple Juice ◽  
Experimental Planning ◽  
Ultra Filtration ◽  
Pineapple Pulp

The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and concentrated in 10 fold the bromelain extract. SDS-PAGE electrophoresis showed that the ultra-filtrated had high purity and the bromelain from A. comosus pulp had a molecular weight of 24.5 kDa.

scholarly journals Physiochemical and Organoleptic Evaluation of Drink Produced from Pineapple (Ananas comosus) and Tigernut (Cyperus esculentus)

2020 ◽  
pp. 1-8
Author(s):  
Adelakun, Oluyemisi Elizabeth ◽  
Taiwo, Omotayo Tijesuni
Keyword(s):  
Sensory Evaluation ◽  
Ananas Comosus ◽  
Local Market ◽  
Cyperus Esculentus ◽  
Organoleptic Evaluation ◽  
Physiochemical Properties ◽  
Overall Acceptability ◽  
Pineapple Juice ◽  
Pineapple Fruit ◽  
Therapeutic Properties

Pineapple, one of the most popular tropical fruits is largely desired for its attractive flavor and can be processed into juice. Fruit juice blends can be produced from various fruits or food materials such as tigernut an underutilized tuber, in order to give better quality drink nutritionally and organoleptically. This work therefore evaluated some quality attributes of the beverage produced from tigernut and pineapple. Tigernut and Pineapple fruit were purchased from local market at Ogbomoso. Single strength juice was produced from pineapple fruit while the tigernut milky extract was produced. The pineapple juice was blended with tigernut milky extract in different proportions (95:5, 90:10, 85:15, 80:20 and 75:25%) while 100% pineapple juice served as the control and were analyzed for physiochemical composition and sensory evaluation (Colour, flavor, taste and overall acceptability). The data obtained were subjected to statistical analysis. The results showed that the physiochemical properties of juice compared relatively well with the control and sensory evaluation showed that all the juice samples were well acceptable in terms of colour, flavour, taste and overall acceptability. The juice blend could be recommended to every household for its nutritional and therapeutic properties.

scholarly journals UJI DAYA HAMBAT ANTIBAKTERI AIR PERASAN DAGING BUAH NANAS (ANANAS COMOSUS (L) MERR VAR. QUEEN) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES

2020 ◽  
Vol 20 (1) ◽  
pp. 87
Author(s):  
Sarwendah Sarwendah ◽  
Yusliana Yusliana ◽  
Heronimus Candra G Laia ◽  
Pieter Julius Daely ◽  
Linda Chiuman
Keyword(s):  
Propionibacterium Acnes ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Ananas Comosus ◽  
Pineapple Juice ◽  
Pineapple Fruit ◽  
Strong Antibacterial Effect ◽  
Paper Disc

Abstrak: Buah nanas (Ananas comosus (L) Merr) merupakan salah satu jenis buah-buahan tropis yang banyak diminati masyarakat. Buah nanas biasa dingunakan masyarakat sebagai antibakteri, antiinflamasi, antikoagulan, dan antikanker. Tujuan dari penelitian ini untuk mengetahui uji daya hambat anti bakteri air perasan daging buah nanas (Ananas comosus (L) Merr Var.  Queen) terhadap bakteri Propionibacterium acnes. Penelitian ini merupakan penelitian eksperimental laboratorium dengan menggunakan metode difusi kertas cakram. Hasil dari penelitian menunjukkan bahwa air perasan daging buah nanas (Ananas comosus (L) Merr Var. Queen) pada konsentrasi 25%, 50%, 75%, 100% memiliki efektivitas sedang sebagai antibakteri dalam menghambat pertumbuhan bakteri Propionibacterium acnes, pada kontrol positif yang menggunakan klindamisin dengan rata-rata diameter zona hambat sebesar 25,8 mm memiliki pengaruh antibakteri yang sangat kuat dan untuk kontrol negatif dengan menggunakan aquades tidak menunjukkan zona hambat 0 mm pada bakteri Propionibacterium acnes.Kata kunci: Buah nanas, propionibacterium acnes, daya hambat antibakteri.Abstract: Pineapple (Ananas comosus (L) Merr) is one of the most popular fruit among people, Pineapple is used as antibacterial, anti-inlfammatory, anticoagulant and anticancer. The purpose of this study is to determine the antibacterial inhibitory test of pineapple juice (Ananas comosus (L) Merr Var. Queen) against Propionibacterium acnes. This research is a laboratory experimental using paper disc diffisuion method. The results of this study indicate that pineapple juice at concentration of 25%, 50%, 75%, 100% it has a moderately antibacterial effectiveness in inhibit the growth of Propionibacterium acnes, positive control using clindamycin with an average inhibition zone diameter of 25.8 mm has a very strong antibacterial effect and for negative control using aquades does not show 0 mm inhibition zone in Propionibacterium acnes bacteria.Keywords: Pineapple fruit, propionibacterium acnes, antibacterial inhibition.

scholarly journals Physical and Organoleptic Qualities of Milk-Based Caramel with Variations in Addition of Pineapple Fruit (Ananas comosus (L) Merr.)

2021 ◽  
Vol 16 (4) ◽  
pp. 354-361
Author(s):  
D. L. Rukmi ◽  
M. N. A. Muslim ◽  
E. C. Wulandari ◽  
S. Mulyani ◽  
A. M. Legowo
Keyword(s):  
Water Content ◽  
Analysis Of Variance ◽  
Fiber Content ◽  
Crude Fiber ◽  
Ananas Comosus ◽  
Physical Quality ◽  
Pineapple Juice ◽  
Crude Fiber Content ◽  
Randomized Design ◽  
Pineapple Fruit

The purpose of this study was to determine the physical and organoleptic qualities of milk-based caramel with variations in the use of pineapple (Ananas comosus (L) Merr.). The design of this study used a completely randomized design (CRD) with five treatments and four replications. The tested treatments include milk-based caramel, which was processed with pineapple juice at T0=0%; T1=5%; T2=10%; T3=15%; T4=20%. The research variables included physical quality testing consisting of water content and crude fiber content, further testing of texture, color, and panelists' preference as organoleptic parameters tested using sensory tests. The observations obtained will be processed using analysis of variance (ANOVA) with a level of 5% and will be further tested using Duncan's Multiple Range Test (DMRT) if it shows a significant effect. Based on the results of analysis of variance (ANOVA), the variation in various treatments with the addition of pineapple fruit in the milk-based caramel processing showed a significant effect (P<0.05) on the physical quality of caramel (crude fiber content and water content) and organoleptic quality (texture, color), and preference) on milk-based caramel products. The percentage of crude fiber content is 0.35%-4.50%; moisture content 10.15%-15.63%; texture score is hard to not hard; the color score is light brown to brown and liking score is slightly like to like. The conclusion of this research was the addition of pineapple affects crude fiber content, milk caramel water content, texture, color, and milk caramel preference.

scholarly journals Uji Daya Hambat Anti Bakteri Air Perasan Daging Buah Nanas (Ananas Comosus (L) Merr Var. Queen) terhadap Bakteri Eschericia Coli

2019 ◽  
Vol 19 (2) ◽  
pp. 239
Author(s):  
Pieter Julius Daely ◽  
Sarwendah Sarwendah ◽  
Heronimus C.G Laia ◽  
Suhartina Damardi
Keyword(s):  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Laboratory Method ◽  
Ananas Comosus ◽  
Tropical Fruit ◽  
Pineapple Juice ◽  
Anti Cancer ◽  
Experimental Laboratory ◽  
Pineapple Fruit

Pineapple fruit (Ananas comosus (L) Merr) is one type of tropical fruit that is consumed by many people. Pineapple is also commonly used by the community as an antibacterial, anti-inflammatory, anti-coagulant, and anti-cancer. The purpose of this study was to determine the effectiveness of the antibacterial inhibitory effect of pineapple juice (Ananas comosus (L) Merr Var. Queen) on Eschericia coli bacteria. This study used an experimental laboratory method with disc diffusion and using ciprofloxacin disc as a positive control and distilled water as a negative control. Based on the results of research that has been done, it can be concluded that pineapple juice (Ananas comosus (L) Merr Var. Queen) at a concentration of 25%, 50%, 75%, and 100% has the effectiveness as an antibacterial in inhibiting the growth of Eschericia coli bacteria.

scholarly journals EFFECT OF CONCENTRATION OF UREA (AMMONIUM SULFATE) FERTILIZER ON MAKING NATA DE PINA FROM PINEAPPLE JUICE (ANANAS COMOSUS L. MERR)

2019 ◽  
Vol 7 (2) ◽  
pp. 89-96
Author(s):  
Mulawi ◽  
Ayu Kristina
Keyword(s):  
Ammonium Sulfate ◽  
Fermentation Process ◽  
Glacial Acetic Acid ◽  
Acetobacter Xylinum ◽  
Ananas Comosus ◽  
Urea Fertilizer ◽  
Pineapple Juice ◽  
Cooking Process ◽  
Pineapple Fruit ◽  
Harvesting Process

This study aims to explain the effect of the concentration of urea fertilizer (Ammonium Sulfate) on the manufacturing of Nata De Pina. This trial aims to compare different concentrations of urea (Ammonium Sulfate) fertilizer. The parameter observed was the weight of Nata De Pina. This research was conducted in six stages, namely: (1) preparing pineapple, (2) fineness, (3) cooking, (4) cooling, (5) addition of acetobacter xylinum and fermentation, (6) harvesting and measurement of Nata De Pina . Based on the research, it was produced that the way to make Nata De Pina can be done by preparing ripe pineapple fruit and cleaning it and then cutting it into small pieces and mashed using a blender to extract the pineapple juice. When blended, the ratio between pineapple and water is 1 kg of pineapple with 1 liter of water. Then, filtered to take water or pineapple juice. In addition, other ingredients are also used in the cooking process such as urea fertilizer (Ammonium Sulfate) with different concentrations, sugar, glacial acetic acid, and acetobacter xylinum bacteria. After all the ingredients have been prepared, then do the cooking with a small flame. If it boils, add all the other ingredients except the acetobacter xylinum bacterium and leave it for 2-3 minutes after which the baking sheet is prepared to cool down, when the Nata De Pina solution has cooled down, put in 10 ml of acetobacter xylinum bacteria. After that, the surface of the baking sheet is tightly closed using newspaper and allowed to stand for 14 days for the fermentation process to become a Nata De Pina product. Then the harvesting process of Nata De Pina is carried out and the weight is measured. Nata De Pina with greater weight parameters and good quality is shown in Nata De Pina with the highest concentration of urea (ammonium sulphate) fertilizer, which is 2.5 grams. So in this case, the concentration of urea (Ammonium Sulfate) fertilizer suitable for making Nata De Pina is 2.5 grams.

scholarly journals The Use of Pineapple Fruit in Batting Process on the Chemical Properties of Sheepskin Using Shorfloat Tanning

2021 ◽  
Vol 16 (3) ◽  
pp. 166-173
Author(s):  
Roselini Agustia Andini ◽  
Mustakim Mustakim ◽  
Imam Thohari
Keyword(s):  
Protein Content ◽  
Chemical Properties ◽  
Enzyme Concentration ◽  
Ananas Comosus ◽  
Shrinkage Temperature ◽  
Pineapple Juice ◽  
Randomized Design ◽  
Tanning Process ◽  
Pineapple Fruit

Batting is the process of removing globular and non-collagen proteins. Bromelain can be found in all parts of the pineapple plant (Ananas comosus L.) with different amounts and properties. Bromelain can be used as a batting agent in the tanning process. The purpose of this study was to determine the effect of the bromelain enzyme concentration used during the protein removal process on the quality of sheepskin. This research used 30 sheets of sheepskin obtained from abattoir in Yogyakarta City and pineapple fruit’s flesh. The experimental design used was a completely randomized design. The research uses different concentrations of pineapples fruit’s flesh (T1 0,5%, T2 1%, T3 1,5% and T4 2% w/w) with feliderm (T0). Statistical analysis using one-way analysis of variance and in accordance with the statistical model. The results of this study showed in dissolved protein content was T0 0.83%, T1 0.77%, T2 0.68%, T3 0.82% and T4 0.85%, protein content of batting skin was T0 19.17%, T1 17.90%, T2 18.37%, T3 21.35% and T4 21.50%, protein content of leather was T0 59.69%, T1 59.27%, T2 60.34%, T3 61.59% and T4 63.25%, fat content was T0 15.31%, T1 14.61%, T2 15.19%, T3 15.36% and T4 16.25%, chromium content was T0 2.60%, T1 2.67%, T2 2.78%, T3 2.82% and T4 3.00% and shrinkage temperature was T0 92.330C, T1 95.670C, T2 93.670C, T3 96.330C and T4 97.330C. The results for pineapple juice showed potency to be used as batting agent for sheepskin. The minimum amount of pineapple juice added for batting the sheepskin is 1% (w/w).

"Recent Patents on the Triboelectrostatic Separation of Fly Ash"

2019 ◽  
Vol 13 ◽  
Author(s):  
Haisheng Li ◽  
Wenping Wang ◽  
Yinghua Chen ◽  
Xinxi Zhang ◽  
Chaoyong Li
Keyword(s):  
Fly Ash ◽  
Power Plants ◽  
High Efficiency ◽  
Separation Efficiency ◽  
High Reliability ◽  
Operating Conditions ◽  
Security Requirements ◽  
Unburned Carbon ◽  
Efficient Operation ◽  
Triboelectrostatic Separation

Background: The fly ash produced by coal-fired power plants is an industrial waste. The environmental pollution problems caused by fly ash have been widely of public environmental concern. As a waste of recoverable resources, it can be used in the field of building materials, agricultural fertilizers, environmental materials, new materials, etc. Unburned carbon content in fly ash has an influence on the performance of resource reuse products. Therefore, it is the key to remove unburned carbon from fly ash. As a physical method, triboelectrostatic separation technology has been widely used because of obvious advantages, such as high-efficiency, simple process, high reliability, without water resources consumption and secondary pollution. Objective: The related patents of fly ash triboelectrostatic separation had been reviewed. The structural characteristics and working principle of these patents are analyzed in detail. The results can provide some meaningful references for the improvement of separation efficiency and optimal design. Methods: Based on the comparative analysis for the latest patents related to fly ash triboelectrostatic separation, the future development is presented. Results: The patents focused on the charging efficiency and separation efficiency. Studies show that remarkable improvements have been achieved for the fly ash triboelectrostatic separation. Some patents have been used in industrial production. Conclusion: According to the current technology status, the researches related to process optimization and anti-interference ability will be beneficial to overcome the influence of operating conditions and complex environment, and meet system security requirements. The intelligent control can not only ensure the process continuity and stability, but also realize the efficient operation and management automatically. Meanwhile, the researchers should pay more attention to the resource utilization of fly ash processed by triboelectrostatic separation.

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