scholarly journals Response Surface Optimization for Antioxidant Extraction and Attributes Liking from Roasted Rice Germ Flavored Herbal Tea

Processes ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 125
Author(s):  
Sriwiang Rittisak ◽  
Ratchanee Charoen ◽  
Natthaya Choosuk ◽  
Wanticha Savedboworn ◽  
Wiboon Riansa-ngawong

The optimal process conditions when examining the antioxidant potential, total polyphenol content, and attribute liking in roasted rice germ flavored herbal tea were investigated using response surface methodology (RSM). The influence upon the extraction process of time and temperature was assessed using a full factorial design on three levels with two variables (32), involving five central point replicates. Extraction temperature (70 °C, 80 °C, and 90 °C) and extraction time (3 min, 4.5 min, and 6 min) served as independent variables, while the dependent variables were allocated to the regression equation to determine antioxidant activity (R2 = 0.941) along with total polyphenol content (R2 = 0.849), flavor liking score (R2 = 0.758), and overall liking score (R2 = 0.816). Following experimentation, it was determined that the optimal time and temperature conditions to maximize total polyphenol content, antioxidant activity, flavor, and overall liking score were in a range of 86 °C to 90 °C for 3.4 min to 5.9 min. When these conditions were imposed, the antioxidant potential, total polyphenol content, flavor, and overall liking score were >70% for DPPH scavenging activity, >75 mgGAE/g, >6.7 (like moderately), and >6.5 (like moderately), respectively.

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 489 ◽  
Author(s):  
Xuan Tien Le ◽  
Vo Luu Lan Vi ◽  
Tran Quoc Toan ◽  
Long Giang Bach ◽  
Tran Thanh Truc ◽  
...  

This research aimed to optimize the total polyphenol content (TPC) extracted from soybean sprout powder under different experimental parameters, including ethanol concentration (60–100% v/v), extraction temperature (40–80 °C), extraction time (15–150 min), material:solvent ratio (1:4–1:10 g/mL), the number extraction cycles (1, 2 and 3 times), the age of sprout (0–7 days), and the used part of the sprout (cotyledon, hypocotyl, or radicle). The obtained results were used in response surface methodology, in combination with a central composite design, to model the total polyphenol content (TPC) with respect to three variables, including ethanol concentration, extraction temperature, and material:solvent ratio. The experimental conditions for optimal recovery of TPC consisted of ethanol concentration of 88% (v/v), extraction temperature of 59 °C, material:solvent ratio of 1:6.5 g/mL, extraction time of 60 min, and 2 cycles of maceration. In addition, for maximal TPC, the sprout should undergo the germination of 5 days and the radicle fraction should be used. Based on the suggested optimum conditions, the obtained and verified TPC was 19.801 mg genistein (GE)/g dry weight (d.w.). The obtained dried extract also exhibited low antioxidant activity.


2020 ◽  
Vol 66 (2) ◽  
Author(s):  
Anna Nowak ◽  
Dominika Szatan ◽  
Joanna Zielonka-Brzezicka ◽  
Katarzyna Florkowska ◽  
Anna Muzykiewicz ◽  
...  

Introduction: Oxidative stress caused by an excess of reactive oxygen species (ROS) is the main reason behind many civilization diseases. The fruits and leaves of Prunus domestica L. contain antioxidants such as vitamins, polyphenols (including flavonoids) and organic acids.The aim of the study was to evaluate the antioxidant activity and total polyphenol content of extracts of leaves and fruits of the plum harvested in 2 ripening phases.Materials and methods: Antioxidative activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) – ABTS methods, and total polyphenol content by Folin–Ciocalteu technique. Extracts in 40%, 70% and 96% (v/v) of ethanol as well as 99.8% (v/v) methanol were used. Ultrasound-assisted extraction was applied for 15, 30 and 60 min.Results: The antioxidant potential of the extracts, measured by DPPH method, was highest for leaf methanolic extracts of plum harvested before ripening (3.83 ±0.01 mg trolox/g of raw material), while for the ABTS method, highest activity was found in the fruit peel extract in methanol (12.89 ±0.04 mg trolox/g raw material). The highest polyphenol levels (5.89 ±0.16, 6.06 ±0.09 and5.76 ±0.13 mg gallic acid/g raw material) were found in the extracts of leaves harvested at fruit ripening stage, prepared in 70% (30 and 60 min) ethanol and 40% ethanol (60 min), respectively.Conclusion: The alcohol based extracts from the leaves and fruit of Prunus domestica L. were characterized by high antioxidant potential, which suggests the possibility of their use as a valuable source of antioxidants. Ethanol seems to be the most appropriate extractant.Keywords: alcohol based extracts; antioxidant activity; Prunus domestica L.; ripening stage; total polyphenol content.


2010 ◽  
Vol 46 (4) ◽  
pp. 651-656 ◽  
Author(s):  
Adriana Bramorski ◽  
Adriana da Rosa Cherem ◽  
Chaiana Paula Marmentini ◽  
Joseane Torresani ◽  
Tatiana Mezadri ◽  
...  

The plant Morinda citrifolia L. (noni) has been the focus of many recent studies due to its potential effects on treatment and prevention of several diseases. However, there are few in vivo and in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine the total polyphenol content (TPC) and antioxidant capacity of a juice commercialized as noni juice, but containing grape, blueberry and noni fruits. Commercial noni juice was compared against its separate constituents of blueberry and grape juice. Folin-Ciocalteu and DPPH• methods were used to determine the concentration of total polyphenol content and antioxidant activity, respectively. Commercial noni juice presented higher values of TPC (91.90 mg of gallic acid/100 mL juice) and antioxidant activity (5.85 mmol/L) compared to its 5% diluted constituents. Concentrated blueberry juice presented higher TPC and antioxidant activity than the other juices analyzed. Considering that the blueberry and grape juices account for only 10% in the composition of commercial noni juice, it can be inferred that these two components contribute significantly to the antioxidant activity. Therefore, additional studies are necessary in order to elucidate the contribution of the noni juice as an antioxidant.


2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Viren Ranawana ◽  
Gordon McDougall ◽  
Nicholas Hayward ◽  
Vassilios Raikos

AbstractCombinable broad beans (Vicia faba) is a major agricultural crop in Europe with annual production around 1.5 million mt. During its milling the seed testa (hull) is removed and is a significant underutilised secondary product. The aim of this study was to examine the nutritional and functional properties of broad bean hull (BBH), specifically for blood glucose control and as a source of fibre for humans.The proximate composition of BBH was assessed using standard AOAC methods and the phytochemical profile using LC-MS(1). BBH extracts were characterised for total polyphenol content, antioxidant potential (FRAP, TEAC)(2), and effects on α-amylase (AA) and α-glucosidase (AG) enzyme activity(3). Bread containing BBH was developed and assessed for glycaemic potential using a standardised in vitro gastro-intestinal digestion model(4). Wheat bran and acarbose were used as comparators.BBH showed a comparable proximate composition to wheat bran but with higher fibre (49 vs 43 g/100g), and lower carbohydrate (19 vs 23 g/100g) and fat contents (1.3 vs 5 g/100g). LC-MS profiling showed that proanthocyanidins were the predominant phytochemicals in the hull with some minor compounds including flavonols. Total polyphenol content and antioxidant potential (FRAP, TEAC) of BBH was significantly higher than in wheat bran (160.1 vs 12.3 μg Gallic acid/mL, 3877.9 vs 75.5 μM Fe Sulphate, and 0.50 vs 0.19 mM Trolox Eq respectively; P < 0.001). Compared to acarbose BBH showed a significantly higher IC50 for α-amylase inhibition (76.6 vs 4.2 μg/mL), and a significantly lower value for α-glucosidase inhibition (2.7 vs 2175.8 μg/mL)(P < 0.001). The addition of BBH significantly reduced starch digestion in bread during the oral, gastric phases and at 20 and 60 min of intestinal digestion (P < 0.005).Broad bean hull is a good source of non-soluble fibre with potential for increasing fibre contents in foods by up to 15g/100 g. Compared to similar material it is a richer source of polyphenols and antioxidants, retards both α-amylase and α-glucosidase activity and shows potential for reducing the glycaemic potential of foods.The work was funded by the Rural and Environment Science and Analytical Services Division of the Scottish government (RESAS).


Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 283 ◽  
Author(s):  
Bogumiła Urbańska ◽  
Jolanta Kowalska

The polyphenol content of cocoa beans and the products derived from them, depend on the regions in which they are grown and the processes to which they are subjected, especially temperature. The aim of the study was to compare the total content of polyphenols and antioxidant activity of chocolates obtained from roasted and unroasted cocoa beans. The chocolates produced from each of the six types of unroasted beans and each of the five types of roasted beans were investigated. The seeds came from Ghana, Venezuela, the Dominican Republic, Colombia and Ecuador. The highest total polyphenol content was determined in cocoa beans originating from Colombia and in the chocolates obtained from them. A higher content of total polyphenols was found in unroasted cocoa beans, which indicates the influence this process had on the studied size. The ability to scavenge free DPPH radicals was at a high level in both the beans and the chocolates produced from them, irrespective of the region where the raw material was grown. A positive correlation between the total polyphenol content and the ability to scavenge free radicals was found.


2014 ◽  
Vol 62 (7) ◽  
pp. 1674-1682 ◽  
Author(s):  
Ante Loncaric ◽  
Krunoslav Dugalic ◽  
Ines Mihaljevic ◽  
Lidija Jakobek ◽  
Vlasta Pilizota

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2557
Author(s):  
Sorin Ciulca ◽  
Gheorghe Roma ◽  
Ersilia Alexa ◽  
Isidora Radulov ◽  
Ileana Cocan ◽  
...  

Fruits of bilberry (Vaccinium myrtillus L.) are valued mainly for their nutraceutical properties, and are among the fruits with the highest antioxidant activity due to their high content of phenolic compounds. The aim of this research was to assess the total polyphenol content and antioxidant activity of fruits in six wild bilberry populations from two regions of Romania over three years. The total polyphenol content was determined according to the Folin–Ciocalteu modified method, while the antioxidant activity was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay. The Padis and Raul Lung populations registered the highest values of polyphenol content and antioxidant activity, as such the fruits of these bilberry populations could be considered potential sources of antioxidants for direct consumption or for use as ingredients for food products or food supplements. Significant variation of total polyphenol content and antioxidant activity was observed both between populations from the same region and from different regions. The low level of broad sense heritability for total polyphenol content and antioxidant activity associated with the high effects of year, and population–year interaction indicates that the accumulation of polyphenols in bilberry fruits is influenced by changes in environmental conditions.


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