Natural Deep Eutectic Solvents (NaDESs) as Alternative Green Extraction Media for Ginger (Zingiber officinale Roscoe)

2021 ◽  
Vol 2 (4) ◽  
pp. 576-599
Author(s):  
Andromachi Tzani ◽  
Styliani Kalafateli ◽  
Grigorios Tatsis ◽  
Maria Bairaktari ◽  
Ioanna Kostopoulou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Zingiber Officinale ◽  
Deep Eutectic Solvents ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimum Conditions ◽  
Natural Deep Eutectic Solvents ◽  
Zingiber Officinale Roscoe

The extraction of valuable phytochemicals from natural sources is an important and constantly evolving research area. Zingiber officinale Roscoe (ginger) contains high amounts of bioactive phytochemicals, which are desirable due to their significant properties. In this work, the ability of different natural deep eutectic solvents (NaDESs) to serve as green solvents for the preparation of high added value extracts from ginger is explored, in combination with ultrasound assisted extraction. The method was optimized by applying a response surface methodology using the NaDES Bet/La/W (1:2:2.5). Three independent variables, namely the extraction time, ultrasound power and NaDES-to-dry-ginger ratio, were investigated by employing a 17-run three-level Box–Behnken Design (BBD) in order to study the correlation between the extraction conditions and the quality of the obtained extracts. The optimum conditions (in order to achieve simultaneously maximum total phenolic content and antioxidant activity), were found to be 23.8 min extraction time, 60 Watt and NaDES/ginger 25:1 w/w. In the optimum conditions the DPPH radical scavenging ability of the extracts was found to reach IC50 = 18.16 mg/mL after 120 min, whereas the TPC was 20.10 ± 0.26 mg GAE/g of dry ginger. The green methodology was also compared with the extraction using conventional solvents. All the obtained extracts were evaluated for their antioxidant activity and their total phenolic content, while the extract derived by the optimum extraction conditions was further investigated for its ability to bind to calf thymus DNA (ctDNA).

Antioxidant and acetylcholinesterase inhibitory activities of ginger root (Zingiber officinale Roscoe) extract

2017 ◽  
Vol 14 (4) ◽  
Author(s):  
Bui Thanh Tung ◽  
Dang Kim Thu ◽  
Nguyen Thi Kim Thu ◽  
Nguyen Thanh Hai
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Zingiber Officinale Roscoe ◽  
Etoac Fraction ◽  
Inhibitory Activities ◽  
Acetylcholinesterase Enzyme

AbstractBackgroundRoscoe has been used in traditional medicine for the treatment of neurological disorder. This study aimed to investigate the phenolic contents, antioxidant, acetylcholinesterase enzyme (AChE) inhibitory activities of different fraction ofMethodsThe roots ofResultsOur data showed that the total phenolic content of EtOAc fraction was highest equivalents to 35.2±1.4 mg quercetin/g of fraction. Our data also demonstrated that EtOAc fraction had the strongest antioxidant activity with ICConclusionsOur results suggest that the EtOAc fraction of

scholarly journals Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

2020 ◽  
Vol 67 (4) ◽  
pp. 1250-1261
Author(s):  
Saniye Akyıl ◽  
Işıl İlter ◽  
Mehmet Koç ◽  
Zeliha Demirel ◽  
Ayşegül Erdoğan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phaeodactylum Tricornutum ◽  
Phenolic Content ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Extraction

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

Modeling of Continuous Ultrasonication to Improve Total Phenolic Content and Antioxidant Activity in Sorghum Flour: A Comparison between Response Surface Methodology and Artificial Neural Network

2017 ◽  
Vol 13 (4) ◽  
Author(s):  
Umesh C. Lohani ◽  
K. Muthukumarappan
Keyword(s):  
Neural Network ◽  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Ann Model ◽  
Optimum Conditions ◽  
Fermentation Time ◽  
Sorghum Flour ◽  
Bound Phenolics

Abstract Fermentation followed by continuous ultrasonication was applied to release the bound phenolics in sorghum flour (SF). Total phenolic content (TPC) and antioxidant activity (AA) increased with decrease in fermentation time (FT), flour to water ratio (FWR), flow rate (FR) and ultrasonication intensity (UI). The influence of process variables was investigated by Box–Behnken design and multi-layer perceptron neural network. The optimum conditions for maximum TPC and AA were obtained as 12 h FT, 10 % (w/v) FWR, 20 W/cm2 UI, 4 ml/s FR and 120 s UT. The values observed for TPC and AA at optimum conditions were 90.1 mg GAE/100 g dm and 190.1 µmol TE/100 g dm, respectively, while these values for control SF were observed as 63.9 mg GAE/100 g dm and 133.5 µmol TE/100 g dm. Ultrasonication improved the free phenolic acid content by releasing bound phenolics in SF. The ANN model prediction was more precise compared to the RSM model.

Analysis of Antioxidant Activity and Total Phenolic Content in Garcinia mangostana Using Response Surface Methodology

2015 ◽  
Vol 1113 ◽  
pp. 217-222
Author(s):  
Ummi Kalthum Ibrahim ◽  
Amira Sofea Mahamad Husin ◽  
Ruzitah Mohd Salleh
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Garcinia Mangostana ◽  
Different Parts

This study investigates the antioxidant activity and total phenolic content on different parts of Garcinia mangostana which are pericarp, leaf, fruit, and seed. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to determine the antioxidant activity and the total phenolic content was determined by Folin-Ciocalteu method. Response surface method (RSM) was used to determine the effect of the two extraction variables: extraction time (30-120 minutes) and extraction temperature (30-50°C) on yield of total phenolic content and antioxidant activity of different parts of Garcinia mangostana. The significant factors on each experimental design response were identified from the analysis of variance (ANOVA). The best condition for total phenolic content of different parts of Garcinia mangostana was chosen based on the desirability obtained by using extraction temperature of 30°C and extraction time of 60.09 minutes, which resulted in 0.0274 mg GAE/g of total phenolic content in pericarp, 0.2501 mg GAE/g of total phenolic content in leaf, 0.0202 mg GAE/g of total phenolic content in fruit, and 0.0198 mg GAE/g of total phenolic content in seed of Garcinia mangostana. Under this conditions, it was found that the antioxidant activity of pericarp, leaf, fruit, and seed of Garcinia mangostana were 89.45%, 86.58%, 93.33%, and 78.80% of radical scavenging activity, respectively.

scholarly journals Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 69 ◽  
Author(s):  
Valentina Pavić ◽  
Dora Flačer ◽  
Martina Jakovljević ◽  
Maja Molnar ◽  
Stela Jokić
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antibacterial Property ◽  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Total Phenolic ◽  
Ruta Graveolens ◽  
Gram Negative ◽  
Natural Deep Eutectic Solvents

Rue (R. graveolens) has been an extensively studied medicinal plant due to its rich phytochemicals content, such as furanocoumarins and flavonoids. The aim of this study was to determine the effects of varying extraction conditions on the total phenolic content, the antioxidant and antibacterial property of rue leaves crude extracts using deep eutectic solvents with different water content. These extraction conditions include the temperature and the extraction time. The extract obtained at 30 °C, with 20% water and at 90 min, with 13.3 µg mL−1 concentration, was found to possess the highest total phenolic content (38.24 ± 0.11 mg of GAE g−1 of DM) and the highest antioxidant activity (72.53 ± 0.31%). In this study, the same extract showed the best antibacterial efficiency against all the tested strains, especially gram-negative P. aeruginosa.

Total Phenolic Content and Antioxidant Activity of Local Fruit Wastes in Malaysia

2015 ◽  
Vol 1113 ◽  
pp. 471-476
Author(s):  
Ummi Kalthum Ibrahim ◽  
Muhammad Ihsan Sobri Yusof ◽  
Khairul Anuar Ahmad Zamil ◽  
Nadia Kamarrudin ◽  
Siti Noor Suzila Maqsood-Ul-Haque ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Fruit Wastes

The purpose of this study is to investigate the antioxidant properties and total phenolic content in local fruit wastes. Watermelon rinds, mango and banana peels were selected in the experiment. Two parameters involved were the extraction time and temperature. All samples had undergone drying process in an oven at 40°C for 24 hours before being extracted with 80%, v/v methanol on a hot plate together with magnetic stirrer. The extraction time was ranged from 20 to 40 minutes and the extraction temperature was from 30 to 60°C. Ferric Reducing Antioxidant Power (FRAP) assay and Folin-Ciocalteu reagent were used for antioxidant analysis and phenolic content determination, respectively. UV-Vis spectrophotometer was used to detect the antioxidant activity at 593 nm and 765 nm for phenolic content. Response Surface Method was used to find the best extraction condition. The best extraction conditions were found at extraction temperature and time of 58.93°C and 37.86 minutes, respectively.

scholarly journals Optimization of Extraction Parameters for Antioxidant and Total Phenolic Content of Ficus carica L. Latex from White Genoa Cultivar

2019 ◽  
Vol 31 (8) ◽  
pp. 1859-1865 ◽  
Author(s):  
M. Shahinuzzaman ◽  
Zahira Yaakob ◽  
Norrakiah Abdullah Sani ◽  
Parul Akhtar ◽  
Md. Zahidul Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Signal To Noise Ratio ◽  
Extraction Time ◽  
Ficus Carica ◽  
Effect Of Temperature ◽  
Total Phenolic ◽  
Solvent Concentration ◽  
Extraction Parameters

In this study, the extraction parameters for antioxidant activity and total phenolic content of Ficus carica L. latex from White Genoa cultivar were optimized using response surface methodology (RSM). Effect of temperature, extraction time and solvent concentration for maceration extraction were investigated. The antioxidant activity was determined with DPPH (1,1-diphenyl-2-picrylhydrazyl) assay and expressed as percent of inhibition. Total phenolic content (TPC) was determined with Folin-Ciocalteu assay and expressed as μg GAE/mL. The optimum points that maximize the DPPH inhibition are 32 °C temperature, 31.50 min extraction time and 65 % ethanol. The optimum points for TPC are 32 °C temperature, 37.50 min extraction time and 70 % ethanol. The predicted DPPH inhibition and TPC at these predicted points are 67.08 % and 313.811 μg GAE/mL respectively. In this model, the F value (26.03 and 20.96), R-Squared value (0.9591 and 0.9497) and signal to noise ratio (13.431 and 12.799) are reasonable to fit the model.

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