Characterization of the Composition and Biological Activity of the Venom from Vespa bicolor Fabricius, a Wasp from South China

We analyzed, for the first time, the major components and biological properties of the venom of Vespa bicolor, a wasp from South China. Using HPLC and SDS-PAGE, combined with LC–MS/MS, MALDI-TOF-MS, and NMR data to analyze V. bicolor venom (VBV), we found that VBV contains three proteins (hyaluronidase A, phospholipase A1 (two isoforms), and antigen 5 protein) with allergenic activity, two unreported proteins (proteins 5 and 6), and two active substances with large quantities (mastoparan-like peptide 12a (Vb-MLP 12a), and 5-hydroxytryptamine (5-HT)). In addition, the antimicrobial activity of VBV was determined, and results showed that it had a significant effect against anaerobic bacteria. The minimum inhibitory concentration and minimum bactericidal concentration for Propionibacterium acnes were 12.5 µg/mL. Unsurprisingly, VBV had strong antioxidant activity because of the abundance of 5-HT. Contrary to other Vespa venom, VBV showed significant anti-inflammatory activity, even at low concentrations (1 µg/mL), and we found that Vb-MLP 12a showed pro-inflammatory activity by promoting the proliferation of RAW 264.7 cells. Cytotoxicity studies showed that VBV had similar antiproliferative effects against all tested tumor cell lines (HepG2, Hela, MCF-7, A549, and SASJ-1), with HepG2 being the most susceptible. Overall, this study on VBV has high clinical importance and promotes the development of Vespa bicolor resources.

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Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro

Steroids ◽

10.1016/j.steroids.2021.108830 ◽

2021 ◽

pp. 108830

Author(s):

Xiaorui Cai ◽

Fei Sha ◽

Chuanyi Zhao ◽

Zhiwei Zheng ◽

Shulin Zhao ◽

...

Keyword(s):

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Anti Inflammatory

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Comparison of the Influence of 45S5 and Cu-Containing 45S5 Bioactive Glass (BG) on the Biological Properties of Novel Polyhydroxyalkanoate (PHA)/BG Composites

Materials ◽

10.3390/ma13112607 ◽

2020 ◽

Vol 13 (11) ◽

pp. 2607 ◽

Cited By ~ 1

Author(s):

Katharina Schuhladen ◽

Barbara Lukasiewicz ◽

Pooja Basnett ◽

Ipsita Roy ◽

Aldo R. Boccaccini

Keyword(s):

Tissue Engineering ◽

Cell Biology ◽

Inorganic Compounds ◽

Release Kinetics ◽

Biological Properties ◽

Long Term Effects ◽

Salt Leaching ◽

Low Concentrations ◽

A Minor ◽

Hard Tissue Engineering

Polyhydroxyalkanoates (PHAs), due to their biodegradable and biocompatible nature and their ability to be formed in complex structures, are excellent candidates for fabricating scaffolds used in tissue engineering. By introducing inorganic compounds, such as bioactive glasses (BGs), the bioactive properties of PHAs can be further improved. In addition to their outstanding bioactivity, BGs can be additionally doped with biological ions, which in turn extend the functionality of the BG-PHA composite. Here, different PHAs were combined with 45S5 BG, which was additionally doped with copper in order to introduce antibacterial and angiogenic properties. The resulting composite was used to produce scaffolds by the salt leaching technique. By performing indirect cell biology tests using stromal cells, a dose-depending effect of the dissolution products released from the BG-PHA scaffolds could be found. In low concentrations, no toxic effect was found. Moreover, in higher concentrations, a minor reduction of cell viability combined with a major increase in VEGF release was measured. This result indicates that the fabricated composite scaffolds are suitable candidates for applications in soft and hard tissue engineering. However, more in-depth studies are necessary to fully understand the release kinetics and the resulting long-term effects of the BG-PHA composites.

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Anti-inflammatory Activity of the Synthetic Chalcone Derivatives: Inhibition of Inducible Nitric Oxide Synthase-Catalyzed Nitric Oxide Production from Lipopolysaccharide-Treated RAW 264.7 Cells

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.30.1450 ◽

2007 ◽

Vol 30 (8) ◽

pp. 1450-1455 ◽

Cited By ~ 43

Author(s):

Young Hoon Kim ◽

Jeongsoo Kim ◽

Haeil Park ◽

Hyun Pyo Kim

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Inducible Nitric Oxide Synthase ◽

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Nitric Oxide Production ◽

Oxide Production ◽

Oxide Synthase ◽

Inducible Nitric Oxide

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RETRACTED: Anti-inflammatory Activity of 4-Arylcoumarins from EndophyticStreptomyces aureofaciensCMUAc130 in Murine Macrophage RAW 264.7 Cells

Immunological Investigations ◽

10.1080/08820130600992115 ◽

2007 ◽

Vol 36 (2) ◽

pp. 203-211 ◽

Cited By ~ 10

Author(s):

Thongchai Taechowisan ◽

Pittaya Tuntiwachwuttikul ◽

Chunhua Lu ◽

Yuemao Shen ◽

Saisamorn Lumyong ◽

...

Keyword(s):

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Murine Macrophage ◽

Anti Inflammatory

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The upper limit of normal for thyrotropin is 3 or 4 milli-int. units/L.

Clinical Chemistry ◽

10.1093/clinchem/31.2.296 ◽

1985 ◽

Vol 31 (2) ◽

pp. 296-298 ◽

Cited By ~ 2

Author(s):

A P Durham

Keyword(s):

Clinical Importance ◽

Upper Limit ◽

Low Concentrations

Abstract Recently Musto et al. (Clin Chem 30: 329-330, 1984) noted that, despite the clinical importance of accurate measurement of thyrotropin at low concentrations, the upper limit of normal is well below the stated upper limit of normal of most commercial tests. Here I amplify their statement.

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Screening for inhibitory activity of volatile oils from Piper spp. on acetylcholinesterase and α-glucosidase

Rodriguésia ◽

10.1590/2175-7860202172079 ◽

2021 ◽

Vol 72 ◽

Author(s):

Luiz Everson da Silva ◽

Camila Confortin ◽

Wanderlei do Amaral ◽

Michele Debiasi Alberton ◽

Milena Paim ◽

...

Keyword(s):

Atlantic Forest ◽

Native Plants ◽

Coastal Region ◽

Biological Properties ◽

Aromatic Plants ◽

Volatile Oils ◽

Renewable Source ◽

Active Constituents ◽

Low Concentrations ◽

Inhibitory Activities

Abstract The Atlantic Forest is regarded a rich source of aromatic plants with a broad spectrum of biological properties. The leaves of seven Piper species were collected from the Atlantic Forest domain in the coastal region of Paraná state in winter (W) and spring (S). The volatile oils were extracted from the leaves through hydrodistillation method. The volatile oils were characterized by means of GC-FID and GC-MS. Sesquiterpenes were among the major compounds in all species studied, with many dozens of representatives. However, very low concentrations of monoterpenes were observed; α-pinene in Piper mosenii and P. cernuum, δ-3-carene in P. rivinoides, camphene and β-pinene in P. cernuum are exceptions. The arylpropanoides myristicine and elemicin were found in considerable amount in P. diospyrifolium (26.2%/S) and P. mosenii (16.4%/W), respectively. These oils were also screened for inhibitory activities against acetylcholinesterase (AChE) and α-glucosidase (AG). For AChE the most active samples were P. diospyrifolium, P. aduncum and P. cernuum, with inhibiting level > 93%. For the less sensitive AG, the most promising candidates were P. diospyrifolium and P. mosenii, with inhibiting level > 65%. These results highlight the importance of native plants as renewable source of new inhibitors for AChE and AG. Further investigation is required to identify the most active constituents or fractions from the selected volatile oils.

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